• Environmental Engineering Research
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• v.24 no.1
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• pp.17-23
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• 2019

This study monitored changes in the level of heterotrophic bacteria in the filtrate and investigated the effect of stagnant water on it, using a batch-operated, gravity-driven membrane system for household water treatment. The filtration test was carried out in the presence and absence of stagnant water in the filtrate line. The results showed that stagnant water accelerated the heterotrophic bacteria levels, measured by heterotrophic plate count, even though the heterotrophic plate count of the filtrate finally increased up to $10^5CFU/mL$ regardless of the presence of stagnant water. When the change in heterotrophic plate count of a batch was monitored over filtration time, heterotrophic plate count of the filtrate rapidly decreased within 5 min for each batch filtration. Biofilm formation on the filtrate line was observed in the presence of stagnant water. The biofilm fully covered the filtrate line and contained numerous microorganisms. During storage after filtration, heterotrophic plate count increased exponentially. To improve the filtrate quality of a filtration-based household water treatment system, therefore, the stagnant water in the filtrate line should be minimized, the filtrate produced at the first 5 min is recommended not to be used as potable water, and the storage of filtrate should be avoided.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2004.04a
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• pp.451-454
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• 2004

The study aims to assess the quality of bank filtrate in relation to streamflow and physico-chemical properties of the stream. Turbidity, pH, temperature and dissolved oxygen (DO) of Nakdong River and riverbank filtrate were statistically analyzed. The physico-chemical properties of riverbank filtrate were measured from irregularly different seven pumping wells every day. Autocorrelation analyses were conducted to the qualities of stream water and bank filtrated water. Temperature, pH and DO of streamflow shows strong linearity and long memory effect, indicating the effect of seasonal air temperature and rainy season. Temperature of riverbank filtrate shows weak linearity and weak memory, indicating differently from the trend of stream temperature. Turbidity of steramflow shows strong linearity and long memory effect, while turbidity of riverbank filtrate indicates weak linearity and weak memory. Cross-correlation analysis shows low relation between turbidity, pH, temperature and DO of riverbank filtrate and those of streamflow. Turbidity of streamflow was largely affected by the streamflow rate, showing a similar trend with autocorrelation function of streamflow rate. The turbidity of riverbank filtrate has a lag time of 25 hours. This indicates that turbidity of streamflow in a dry season has very low effect on the turbidity of riverbank filtrate, and a high turbidity of the stream in a rainy season has a fairly low effect on the turbidity of riverbank filtrate.

• Journal of Korean Society of Water and Wastewater
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• v.17 no.4
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• pp.487-494
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• 2003

In this research, water qualities of river water and bank-filtrate were compared for six months including winter season. The location studied was Galjon area, the downstream of the Nakdong river. The well for bank-filtrate was installed 40 m apart from riverside. Main analytic results of bank-filtrate and river water were summarized as followings; the average concentrations in bank-filtrate were turbidity 0.8NTU, TN 0.4mg/l, $BOD_5$, 0.1mg/l, $KMnO_4$ consumption 1.6mg/l, heterotrophic bacteria 350cfu/ml, Fe 0.5mg/l, Mn 0.99mg/l while the average concentrations in river water were turbidity 6.1NTU, TN 3.9mg/l, $BOD_5$, 3.6mg/l, $KMnO_4$ consumption 11mg/l, heterotrophic bacteria 1,640cfu/ml, Fe 0.28mg/l, Mn 0.04mg/l. Water quality of bank-filtrate was mostly shown a good results than it of river water excepting Fe and Mn. In even basic constituents such as water temperature and pH, bank-filtrate was very settled while river water was extraordinary changable and high. In case of nitrogen, especially, total nitrogen of river water was 3.9mg/l while it of bank-filtrate was 0.4mg/l and its reduction was very high. The reason is that $NH^+_4-N$ among total nitrogen in the river water is nitrified and then denitrified in soil layer when it is pumped up as bank-filtrate. But Fe and Mn caused by the characteristics of soil was very high in bank-filtrate while Mn in river water was particularly very low and settled. As the distance between riverside and well was longer, concentration of Fe and Mn may be went up while its bacteria may be reduced.

• The Plant Pathology Journal
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• v.25 no.4
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• pp.369-375
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• 2009

The potential antiviral effects of the culture filtrates (CF) from Serratia marcescens strain Gsm01 against yellow strain of Cucumber mosaic virus (CMV-Y) were investigated. The culture filtrate of S. marcescens strain Gsm01 applied on Chenopodium amaranticolor showed high inhibitory activity, likewise no necrosis appeared when applied on the tobacco plants 2 days before CMV-Y inoculation. When plants were challenge inoculated with CMV-Y for eighteen days, the disease incidence in plants with culture filtrate of S. marcescens Gsm01 did not exceed 59%, whereas 100% of control plants were severely infected. The results of double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA), reverse transcriptase polymerase chain reaction (RT-PCR), dot blotting, and western blotting showed that culture filtrate treatment highly affected the accumulation of CMV-Y or its CP protein gene in the treated plant leaves. It was also observed that the culture filtrate had no RNase activity on genomic RNAs of CMV-Y, suggesting that culture filtrate may not contain ribosome inactivating proteins (RIPs) or proteins with RNase activity. These data shows that culture filtrate of S. marcescens strain Gsm01 seems to be a promising source of antiviral substance for the practical use.

• Journal of Applied Biological Chemistry
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• v.42 no.1
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• pp.34-39
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• 1999

In search of a method to achieve sustainable agricultural practices, a trickling filter was employed for the piggery wastewater treatment, where rice straw was the support medium in place of more commonly used materials. The filtrate from the trickling filter were applied to a soil, on which orchard grass (Dactylic glomerata L.) was grown followed by amendment of the treated straw medium. Orchard grass was cut twice, and growth parameters and yields were measured. Soil chemical properties before and after harvesting orchard grass were analyzed. Development of the forage crop was greatly enhanced by the application of the filtrate in terms of fresh weight, dry weight, and the absorption of nutrients. Better growth and higher nutrient uptake were found in the second cutting of the orchard grass. Organic matter content, ionic intensity, exchangeable cations, and phosphate of the soil increased with the application of the filtrate. The relative high concentrations of salts in the piggery trickling filtrate, expressed in electrical conductivity and content of sodium, exerted no detrimental effect on the crop and soil.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2006.04a
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• pp.307-311
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• 2006

Chemical analyses were conducted for Nakdong River water and riverbank filtrate in Daesan-Myeon area, Changwon City in 2005. Chemical components show different trends in wet season (June, July, August and September) and dry season (the other months). The patterns of chemical variation are classified into four types. Chemical components belonging Type I, as Na and $HCO_3$, show decrease in concentration during wet season and increase in concentration during dry season for both Nakdong River water and the riverbank filtrate. Chemical components belonging Type II, as Mg and $SO_4$, show decrease in concentration during wet season and increase in concentration during dry season for Nakdong River water while show the opposite trend for the riverbank filtrate. Chemical components belonging Type III, as Cl and $NO_3$, show increase in concentration from the start of wet season and high concentration during dry season for both Nakdong River water and the riverbank filtrate. Chemical components belonging Type IV, as Fe, show increase in concentration during wet season and decrease in concentration during dry season for both Nakdong River water and the riverbank filtrate.

• Journal of Applied Biological Chemistry
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• v.42 no.3
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• pp.140-145
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• 1999

Livestock manure is generally beneficial to soil and crop production when appropriate amount is applied, but excessive application may be detrimental to soil and water environments. A proper protocol of livestock waste treatment is required to manage the quality of soil and water. A trickling filter system using rice straw media was employed to treat piggery wastewater from small-scaled livestock farms as an alternative to the currently available methods. Batches of piggery wastewater were treated with this system, and the byproducts of rice straw media and trickling filtrate were applied to the soil with cultivating rye (Secale cereale L.). Objective of this research was to characterize these byproducts for the sustainable soil amendments and rye production. Both the treated straw medium and filtrate were proven to be effective organic fertilizers for rye plant development, with the enhanced but balanced absorption of nutrients. The synergistic effects of filtrate in addition to straw application did not show, but the filtrate appeared to lead to a higher water content of the plant. No specific nutrient deficiency or toxicity symptom was shown due to the salts derived from the byproducts applied. Chemical parameters of the soil quality were significantly improved with the application of straw medium either with or without the filtrate. Judging from parameters relating to the salt accumulations, such as sodium adsorption ratio (SAR), electrical conductivity (EC), exchangeable sodium percentage (ESP), potassium adsorption ratio (KAR), and residual P concentrations, the byproducts from piggery wastewater exhibited no detrimental effects on soil quality within the ranges of treatments used. In addition to the effectiveness of the rice straw trickling filter system for the small-scaled swine farms, both rice straw medium and filtrate could be recycled for the sustainable soil amendment and plant nutrition.

• Journal of the Korean Society of Tobacco Science
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• v.20 no.2
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• pp.172-177
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• 1998

The culture filtrate of Streptomyces sp. B25, which was identified in this experiment, was tested for the control of tobacco mosaic virus (TMV) with the susceptible tobacco cultivar, NC 82, under the field conditions following the preliminary examination of its characters for TMV control. Control efficacy of the culture filtrate against TMV infection continued over 50% up to 6 days after treatment, and its systemic effect was about 30% of the direct effect. In field conditions control efficacy of the culture filtrate against TMV infection was 95.3 % at 2 weeks after TMV inoculation, and decreased to 58.3 % at 3 weeks after inoculation. Five fold-dilution of the culture filtrate showed about half of the control efficacy by the stock culture filtrate. Analysis of the antibiotic material responsible for the inhibition of TMV infection through nuclear magnetic resonance experiments revealed that the antibiotic is antimycin $A_1$, which is firstly reported as an anti-phytoviral antibiotic in this experiment.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.129-130
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• 2002

Monascus pigment was produced by Monascus species. During Monascus fermentation, citrinin, the mycotoxin was produced with pigments. Citrinin can become a problem for use of monascus pigment as a food colorant. We found adding of S. cerevisiae filtrate during Monascus cultivation could enhance production of red pigment whereas it could reduce citrinin level. When we added the filtrate at 24 hand 48 h, respectively, pigment production increased about 400% and citrinin concentration decreased to 30%. In a glucose medium, there was no special effect by addition of filtrate. On the other hand, the effect was striking in a sucrose medium.

• Journal of the Korean Society of Tobacco Science
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• v.7 no.1
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• pp.3-6
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• 1985

The typical dark brown stripe symptom of bacterial wilt disease was observed in the cuttings of tobacco stem treated with the culture filtrate of virulent Pseudomonas solanaceamm. And the tobacco callus create.4 with that culture filtrate showed deterioration of the callus 2 days after the treatment. On the contrary, the cuttings and the callus treated with the culture filtrate of the avirulent bacteria expressed no typical symptom and vigorous growth respectively. Therefore it was suggested that certain toxin which might be produced by the virulent bacteria could break down tobacco cells.