The regulation of flowering time has crucial implications for plant fitness. MicroRNA156 (miR156) represses the floral transition in Arabidopsis thaliana, but the mechanisms regulating its transcription remain unclear. Here, we show that two AGAMOUS-like proteins, AGL15 and AGL18, act as positive regulators of the expression of MIR156. Small RNA northern blot analysis revealed a significant decrease in the levels of mature miR156 in agl15 agl18 double mutants, but not in the single mutants, suggesting that AGL15 and AGL18 co-regulate miR156 expression. Histochemical analysis further indicated that the double mutants showed a reduction in MIR156 promoter strength. The double mutants also showed reduced abundance of pri-miR156a and pri-miR156c, two of the primary transcripts from MIR156 genes. Electrophoretic mobility shift assays demonstrated that AGL15 directly associated with the CArG motifs in the MIR156a/c promoters. AGL18 did not show binding affinity to the CArG motifs, but pull-down and yeast two-hybrid assays showed that AGL18 forms a heterodimer with AGL15. GFP reporter assays and bimolecular fluorescence complementation (BiFC) showed that AGL15 and AGL18 co-localize in the nucleus and confirmed their in vivo interaction. Overexpression of miR156 did not affect the levels of AGL15 and AGL18 transcripts. Taking these data together, we present a model for the transcriptional regulation of MIR156. In this model, AGL15 and AGL18 may form a complex along with other proteins, and bind to the CArG motifs of the promoters of MIR156 to activate the MIR156 expression.
Park, Kyeong-Soo;Seo, Yong-Gil;Nam, Hae-Sung;Sohn, Seok-Joon;Rhee, Jung-Ae
Journal of Preventive Medicine and Public Health
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v.31
no.2
s.61
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pp.293-309
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1998
The purpose of this study is to compare the level of health-related quality of life and relating factor between institutional elderly and community living elderly. The subjects were 390 from Sanatorium or Nursing home and 467 from the community in Kwangju. The results are followed : 1) A comparison of ADL between two groups, institutional elderly and community living elderly, resulted in that community elderly were more significantly independent in the areas of bathing and transfer than institutional elderly. 2) A comparison of IADL between two groups resulted in that : Community elderly were more independent in the areas of using telephone and transportation, food preparation, house keeping, and doing laundry. Institutional elderly were more independent in the area of handling finances. 3) In the case of poor health-related quality of life, institutional elderly showed 2.4 times in the dimension of physical fitness, 1.8 times in daily activity, 2 times in social activity, 2 times in pain, 26.7 times in social support, and 0.4 times in subjective quality of life higher than community elderly There was no significant differences in the rest of dimensions. 4) In institutional elderly, the analysis of variables related to the health-related quality of life resulted in that; The relating factors were sex, education, and chronic illness in the dimension of physical function. Direct contact with family or significant others in the dimension of social activity. Chronic illness in the dimension of pain and perceived health status. Direct or indirect contact with family or significant others over the phone or through letters in the dimension of social support. 5) The analysis of variables related to the health-related quality of life showed that community elderly has more relating variables in each area than institutional elderly. The relating factors were age, sex, and chronic illness in the dimension of physical function. Education and chronic illness in the dimension of emotional status. Age and chronic illness in the dimension of daily activity and social activity Education and chronic illness in the dimension of pain and perceived health status. Sex, education, family size in the dimension of social support. Education and chronic illness in the dimension of subjective quality-of-life. Throughout general daily activity, community elderly showed more satisfactory results than institutional elderly, but in the subjective area of health-related quality of life, such as subjective quality of life, institutional elderly group showed more positive results. And community elderly had more relating factors than institutional elderly. For the health care of the elderly that focused on quality of life, new approaches considering the characteristics of both group, institutional and community living elderly, are needed.
The purpose of this study was to compare gifted and non-gifted high school students on their perceptions of success, grit, aspirations, and achievement goal orientation. Of the 550 high school students surveyed from 4 high schools (i.e., two schools for gifted, two school for non-gifted) in three metropolitan cities, Korea, 496 (i.e., 153 gifted and 343 non-gifted) completed and returned the questionnaire yielding a total response rate of 90.18%. Measures of students' grit (i.e., consistency of interest, and perseverance of effort), aspirations (i.e., intrinsic aspirations - physical fitness, self-growth, affiliation, community contribution, and extrinsic aspirations - financial success, image, fame), achievement goal orientation (i.e., mastery, performance approach and performance avoidance), and perceptions of success (i.e., Success in life is pretty much determined by forces outside our control.) were administered. It was found that the majority of gifted students and non-gifted students perceived that "success is outside our control.", and chose 'hard-working' as the most influential factor on their success. 60.4% of non-gifted students chose 'wealthy family background' as the other most influential factor, whereas only 48.2% of gifted did. Gifted students had higher 'consistency of interest' and 'mastery goals' than non-gifted students. Hierarchical multiple regression analyses showed that grit (i.e., perseverance of effort) was the crucial contributors for enhancing both gifted and non-gifted students' mastery goals and performance approach goals. Results are discussed in relation to theoretical implications and school settings.
The purpose of this study is to analyze the effect of the Naeil Chaeum deduction system on job satisfaction. The results of the study are summarized as follows. A total of 159 small to medium-sized enterprise workers participated in the deduction for Naeil Chaeum. The data were analyzed using SPSS Statistics 20, which included frequency analysis, factor analysis, reliability analysis, correlation analysis, regression analysis, and post analysis. First, the suitability of the method of the Naeil Chaeum deduction system has the highest positive effect on the job satisfaction. Second, the correlation of job satisfaction after filling in Naeil Chaeum deductions showed that fitness (p <.01, ${\beta}=.307$) and efficiency (p <.01, ${\beta}=.296$) had a significant effect on job satisfaction. Third, the analysis of the difference of job satisfaction according to the academic ability of the workers who were enrolled in Naeil Chaeum deduction system was analyzed that professional college graduates (2.08) were higher than job college graduates (1.99).The purpose of this study is to analyze the impact of the Fill - Down System on the job satisfaction by analyzing the importance of quality of life, job satisfaction, and muscle strength, analyzing the difference according to the demographic characteristics of the workers, And helping to stabilize the work life, and to verify the effectiveness of the study.
This study analyzed the effect of pension insurance on the human resources management of SME workers. As a result of the study, a total of 128 workers were enrolled in 60 SMEs in the Chungnam area who were enrolled in pension insurance. The analysis data were analyzed using frequency analysis, factor analysis, reliability analysis, correlation analysis, regression analysis And post - analysis. First, pension insurance had a significant effect on human resource management, and the correlation between independent variables (clarity, suitability, and satisfaction) and dependent variables (job efficiency) was high....Second, the correlation between pension insurance and job performance is more important than clarity and job performance (.339), fitness and job performance (.541), satisfaction and job performance (.531), job performance and job efficiency, And the turnover intention (.549). Third, there is no statistically significant difference in the analysis of the difference in turnover intention according to the number of years of service of the employees who are enrolled in pension insurance. The purpose of this study is to analyze the impact of pension insurance on human resources management to achieve successful policy objectives by promoting the long - term employment of the core manpower of SMEs and cultivating manpower.
Journal of the Korea Society of Computer and Information
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v.16
no.12
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pp.23-31
/
2011
The simple bacteria cooperative optimization (sBCO) algorithm that we developed as one of optimization algorithms has shown relatively good performances, but their performances were limited by step-by-step movement of individuals at a time. In order to solve this problem, we proposed a new method that assigned a speed to each individual according to its rank and it was confirmed that it improved the performances of sBCO in some degree. In addition to the assigning of speed to the individuals, we employed a new mutation operation that most existing evolutionary algorithms used in order to enhance the performances of sBCO in this paper. A specific percent of bad individuals are mutated within an area that is proportion to the rank of the individual in the mutation operation. That is, Gaussian noise of large standard deviation is added as the fitness of individuals is low. From this, the probability that the individuals with lower ranks can be located far from its parent will be increased. This causes that the probability of falling into local optimum areas is decreased and the probability of fast escaping the local optimum areas is increased. From experimental results with four function optimization problems, we showed that the performances of sBCO with mutation operation and individual speed were increased. If the optimization function is quite complex, however, the performances are not always better. We should devise a new method for solving this problem as a further work.
The purpose of this study is to examine the effect of exchange rate volatility on Trading Volume of Container of Korea, and to induce policy implication in the contex of GARCH and regression model. In order to test whether time series data is stationary and the model is fitness or not, we put in operation unit root test, cointegration test. And we apply impulse response functions and variance decomposition to the structural model to estimate dynamic short run behavior of variables. The major empirical results of the study show that the increase in exchange rate volatility exerts a significant negative effect on Trading Volume of Container in long run. The results Granger causality based on an error correction model indicate that uni-directional causality between trading volume of container and exchange rate volatility is detected. This study applies impulse response function and variance decompositions to get additional information regarding the Trading Volume of Container to shocks in exchange rate volatility. The results indicate that the impact of exchange rate volatility on Trading Volume of Container is negative and converges on a stable negative equilibrium in short-run. Th exchange rate volatility have a large impact on variance of Trading Volume of Container, the effect of exchange rate volatility is small in very short run but become larger with time. We can infer policy suggestion as follows; we must make a stable policy of exchange rate to get more Trading Volume of Container
It is well documented that physiological and nutritional condition of wintering birds is strongly related to migration success to breeding sites, and also breeding success. However, how abiotic factors during winter affect the migration and breeding successes still remains unclear. Thus, this study developed a dynamic-state-dependent model for wintering life-history to identify the potential impact on the life-history, success to breeding site and breeding success of wintering birds, which are related to temperature fluctuation, interspecific competition and human disturbance at the wintering sites. To find the best-fit-model, we referred to the existing research data on wintering ecology of Red-crowned cranes (Grus japonensis) in Cheolwon, Korea, which is well documented as a long-term wintering study. Our model predicted that the higher temperature fluctuation and a higher rate of human disturbance are negatively related to migration success to breeding sites and their fitness, ultimately breeding success via changing of proportion in resource allocation (for e. g., lower energy compensation or higher level of stress accumulation). Particularly, the rate of body mass compensation after arrival at wintering sites may be accelerated when there are less temperature fluctuations and a lower rate of human disturbance. In addition, the rate of interspecific competition sharing the wintering foraging sites is negatively related to the rate of body mass compensation. Consequently, we discussed the conservation strategies of wintering birds based on the outcomes of the model.
Life history of Ivela auripes (Lepidoptera: Lymantriidae), a pest of Cornus controversa, was investigated both in laboratory and field condition. At $24{\pm}1^{\circ}C$, developmental period from larvae to adult and adult life span was $26.3{\pm}0.3$ and $4.4{\pm}0.2$ days, respectively. Developmental period of pupae reared in the laboratory was not significantly different from those collected in the field. Female moths reared in the laboratory laid significantly less eggs than those emerged from the pupae collected in the field. Light trap catches was less effective than direct monitoring in the field. The two monitoring results showed that adult moth emerged from early June to late June, and the time of 50% cumulative emergence was 17 June.
Journal of rehabilitation welfare engineering & assistive technology
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v.12
no.1
/
pp.53-62
/
2018
In this study, we developed a smart 1RM system for national health management and physical fitness, which enables quantitative 1RM measurement in various types of exercise using digital pulley technology, and to test the effect on training by using it. We developed the smart 1RM system, which is composed of portable muscle strength measuring device, Bluetooth communication based mobile phone data transmission and circuit diagram, and height adjustable system body. We recruited the 30 participants with 20th aged and divided into training and non-performing groups with 15 participants randomly. The participants performed 5 sets of elbow, lumbar, knee extension / flexion 10 times using smart 1RM system and the experimental period was 3 days a week for a total of 8 weeks. The experimental results showed that the maximum strength of the elbow, lumbar, and knee joints was significantly improved before and after maximal muscle strength training in the training group. Oxygen intakes during 1RM exercise mode showed 10.91% than endurance. To verify the validity of the smart 1RM maximal strength data, the reliability was 0.895 (* p <0.00). This study can be applied to the early rehabilitation treatment of the elderly and rehabilitation patients more quantitatively using the national health care.
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