• BMB Reports
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• 제46권1호
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• pp.53-58
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• 2013

We constructed deletion mutants and seven point mutants by polymerase chain reaction to investigate the specificity of feline foamy virus integrase functional domains. Complementation reactions were performed for three enzymatic activities such as 3'-end processing, strand transfer, and disintegration. The complementation reactions with deletion mutants showed several activities for 3'-end processing and strand transfer. The conserved central domain and the combination of the N-terminal or C-terminal domains increased disintegration activity significantly. In the complementation reactions between deletion and point mutants, the combination between D107V and deletion mutants revealed 3'-end processing activities, but the combination with others did not have any activity, including strand transfer activities. Disintegration activity increased evenly, except the combination with glutamic acid 200. These results suggest that an intact central domain mediates enzymatic activities but fails to show these activities in the absence of the N-terminal or C-terminal domains.

• Journal of Microbiology and Biotechnology
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• 제14권5호
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• pp.1063-1066
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• 2004

The rpoS gene product is a global transcriptional factor, which is involved in bacterial survival under various stress conditions. An rpoS-homologous gene was cloned from a septicemia-causing pathogenic Vibrio vulnificus. Introduction of this gene as a multicopy plasmid into various E. coli strains displayed functional complementation, for examples, increased survivability of an rpoS-defective E. coli cell and induction of known $\delta^S$-dependent, stress-responding promoters of E. coli genes.

• International Journal of Advanced Culture Technology
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• 제9권3호
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• pp.112-116
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• 2021

The aim of this study is to analyze the complementizer that-deletion in embedded complement clauses in English. This paper is concerned with the alternation between the overt that-complementizer and the zero complementizer by the complementizer deletion (C-deletion or that-deletion) in constructions with a nominal complement that-clause, i.e. [VP Verb [CP that-TP]]. In this paper, we compare that-complementation and zero-complementation in a diachronic grammaticalization and corpus, and show that the complementizer that has its origin in pronouns diachronically and finally becomes to form a C-head of the functional category CP. We provide the syntactic and semantic explanation on the optionality of that-deletion while answering the question why and how that-deletion is getting increasing in use especially with the verb, think, in the informal contexts. With the major causes for the currently increasing use of that-deletion, we are concerned with the contexts in which the overt complementizers or the covert complementizers are preferred.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
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• pp.218.1-218
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• 2005

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.278.1-278
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• 1999

No Abstract, See Full Text

• Journal of Microbiology and Biotechnology
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• 제21권1호
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• pp.14-19
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• 2011

A cyclic undecapeptide-family natural product, cyclosporin A (CyA), which is one of the most valuable immunosuppressive drugs, is produced nonribosomally by a multifunctional cyclosporin synthetase enzyme complex in a filamentous fungal strain named Tolypocladium niveum. Previously, structural modifications of cyclosporins such as a regionspecific hydroxylation at the $4^{th}$ N-methyl leucine in a rare actinomycetes called Sebekia benihana were reported to lead to dramatic changes in their bioactive spectra. However, the reason behind this change could not be determined since a system to genetically manipulate S. benihana has not yet been developed. To address this limitation, in this study, we utilized the most commonly practiced gene manipulation techniques including conjugation-based foreign gene transfer-and-expression as well as targeted gene disruption to genetically manipulate S. benihana. Using these optimized genetic manipulation systems, a putative cytochrome P450 hydroxylase (CYP) gene named CYP506, which is involved in CyA hydroxylation in S. benihana, was specifically disrupted and genetically complemented. The S. benihana${\Delta}$CYP506 exhibited a significantly reduced CyA hydroxylation yield as well as considerable yield restoration by functional complementation of the S. benihana CYP506 gene, suggesting that the genetically manipulated S. benihana CYP mutant strains may serve as a more efficient bioconversion host for various valuable metabolites including CyA.

• 생명과학회지
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• 제23권1호
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• pp.1-7
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• 2013

작물의 생산성 증가를 위해 염해 저항성 메커니즘을 이해하는 것이 중요하다. 식물의 염해 저항성에 관련된 유전자를 확보하기 위한 여러 가지의 선별방법이 개발되었다. 본 논문에서는 애기장대의 cDNA 라이브리를 염해감수성 효모인 cnb 돌연변이체에 삽입하여 염해 감수성 표현형을 회복하는 콜로니를 선발하였다. 이 선별방법을 통하여 34종의 cnb 돌연변이체의 염해 감수성을 회복하는 콜로니를 선별하였으며, 염기서열분석을 통하여 CaS와 AtSUMO1, AtHB-12 등 9종의 유전자임을 확인하였다. 이들 유전자 중 CaS의 발현이 염해 저항성을 증가시키는 것과 염해 처리에 의해 CaS의 유전자의 발현이 증가되는 것을 확인하였다. CaS 발현억제 형질전환체는 100 mM 염처리에 의하여 뿌리생장이 저해되었다. 또한 150 mM 염처리에 의하여 CaS 발현억제 형질전환체의 잎에서 백화현상을 나타내었다. 이러한 결과를 통하여 CaS 유전자가 효모와 식물에서 염해 저항성에 중요한 유전자임을 증명하였다.

• Molecules and Cells
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• 제23권3호
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• pp.405-409
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• 2007

A putative type-I chalcone isomerase (CHI) cDNA clone EuNOD-CHI was previously isolated from the root nodule of Elaeagnus umbellata [Kim et al. (2003)]. To see if it encodes a functional CHI, we ectopically overexpressed it in the Arabidopsis (Arabidopsis thaliana) transparent testa 5 (tt5) mutant, which is defective in naringenin production and has yellow seeds due to proanthocyanidin deficiency. Ectopic overexpression of EuNOD-CHI resulted in recovery of normal seed coat color. Naringenin produced by CHI from naringenin chalcone was detected in the transgenic lines like in the wild-type, whereas it was absent from the tt5 mutant. We conclude that EuNOD-CHI encodes a functional type-I CHI. In situ hybridization revealed that EuNOD-CHI expression is localized to the infected cells of the fixation zone in root nodules.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.413-413
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• 2005

• Asian Pacific Journal of Cancer Prevention
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• 제14권3호
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• pp.1847-1850
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• 2013

We conducted a hospital case-control study by genotyping four potential functional single nucleotide polymorphisms (SNPs) to assess the association of Xeroderma pigmentosum complementation group F (XPF) with gastric cancer susceptibility, and role of XPF polymorphisms in combination with H.pylori infection in risk definition. A total of 331 patients with gastric cancer and 355 controls were collected. Four SNPs of XPF, rs180067, rs1799801, rs2276466 and rs744154, were genotyped by Taqman real-time PCR method with a 7900 HT sequence detector system. The gastric cancer patients were more likely to have smoking habit, a family history of cancer and H.pylori infection. We did not find any significant difference in the genotype distributions of XPF rs180067, rs1799801, rs2276466 and rs744154 between cases and controls. However, multivariate logistic analysis showed a non-significant decreased risk in patients carrying rs180067 G allele, rs1799801 T allele or rs2276466 T allele genotypes. A non-significant increased risk of gastric cancer was found in individuals carrying the rs744154 GG genotype. Stratification by H.pylori infection and smoking was not significantly different in polymorphisms of XPF rs180067, rs1799801, rs2276466 and rs744154. The four XPF SNPs did not show significant interaction with H.pylori infection and smoking status (P for interaction was 0.35 and 0.18, respectively). Our study indicated that polymorphisms in rs180067, rs1799801, rs2276466 and rs744154 may affect the risk of gastric cancer but further large sample size studies are needed to validate any association.