• Title, Summary, Keyword: gamma irradiation

Search Result 1,571, Processing Time 0.038 seconds

Effect of Gamma Irradiation on Botrytis cinerea Causing Gray Mold and Cut Chrysanthemum Flowers

  • Chu, Eun-Hee;Shin, Eun-Jung;Park, Hae-Jun;Jeong, Rae-Dong
    • Research in Plant Disease
    • /
    • v.21 no.3
    • /
    • pp.193-200
    • /
    • 2015
  • Gray mold caused by Botrytis cinerea is one of the most important postharvest fungal pathogens of cut flowers. Here, gamma irradiation, an alternative for phytosanitary purposes, and sodium dichloroisocyanurate (NaDCC) were used to control B. cinerea in a cut chrysanthemum (Chrysanthemum morifolium Ramat.) cultivar, 'Baekma', one of the cultivars susceptible to B. cinerea. Spore germination and mycelium growth of B. cinerea were inhibited by gamma irradiation in an inversely dose-dependent manner. A dose of 4 kGy completely inhibited the mycelium growth of B. cinerea. A significant change in flower quality (physical properties) on chrysanthemum was shown from gamma irradiation at over 0.2 kGy (p<0.05). Therefore, in this study, the integration of gamma ray (below 0.2 kGy) and NaDCC, an eco-friendly form of chlorine, was investigated to control the disease with low dose of gamma irradiation dose. Interestingly, the gamma irradiated flowers showed more disease severity than the non-irradiated flowers. The combined treatment of gamma irradiation and NaDCC does not affect the severity of the fungal disease, whereas only 70 ppm of NaDCC treatment showed a significantly reduced severity. These results suggest that only chlorination treatment can be applied to control B. cinerea in cut chrysanthemum flowers.

Effects of pH and Gamma Irradiation on the Physicochemical Properties of Corn Starch

  • Kang, Il-Jun;Chung, Cha-Kwon;Sohn, Jeong-In
    • Preventive Nutrition and Food Science
    • /
    • v.4 no.3
    • /
    • pp.175-179
    • /
    • 1999
  • To develop a production method for modified starches with less pollution, pH adjustment and gamma irradiation were applied to commerical corn starch. Blue values were significantly decreased , while alkali number, optical transmittance and solubility markedly increased when gamma irradiation was applied to pH 2 adjusted corn starch. Water binding capacity and swelling power at pH 5 were the highest among the samples. Gelatinization viscosity was considerably affected by gamma irradiation and pH of the starch. Gamma irradiation of pH 2 adjusted starch showed the lowest peak viscosity and the best cooling stability among the tested samples . Therefore, the production of modified starch with low viscosity as well as with sufficient viscosity stability seems feasible by controlling the pH of the starch and gamma irrdiation.

  • PDF

Effects of External Whole-Body gamma-Irradiation on Embryos in Mice (Cobalt-60 gamma 선(線) 외부전신조사(外部全身照射)가 생쥐의 태아(胎兒)에 미치는 영향(影響))

  • Sung, Jai Ki
    • Korean Journal of Veterinary Research
    • /
    • v.15 no.1
    • /
    • pp.9-13
    • /
    • 1975
  • In oder to investigate the effects of cobalt-60 gamma irradiation on the postnatal body gains and the other influece of the pregnant mice were subjected to single whole-body gamma irradiation externally. The results obtained were as follows: 1. The all mice of 400, 600, and 800 Rads gamma-irradiated groups showed abortion after prenatal gamma irradiation within 4 to 9 days. 2. There were significant differences of the postnatal body gains of mice between the control and treated groups (100 Rads and 200 Rads gamma irradiation). 3. The relation between gamma-irradiated doses and the postnatal growth rates of mice were inversly proportional.

  • PDF

Effects of Gamma Irradiation on Nutrient Composition, Anti-nutritional Factors, In vitro Digestibility and Ruminal Degradation of Whole Cotton Seed

  • Hahm, Sahng-Wook;Son, Heyin;Kim, Wook;Oh, Young-Kyoon;Son, Yong-Suk
    • Journal of Animal Science and Technology
    • /
    • v.55 no.2
    • /
    • pp.123-130
    • /
    • 2013
  • Whole cotton seed (WCS) has become one of the major feed ingredients in TMR for dairy cattle in Korea, and WCS for feed use is mostly imported from abroad. Since this genetically modified oil seed is usually fed to the animal in raw state, its germination ability, if last long, often causes concerns about ecological disturbances. In the process of looking for effective conditions to remove germination ability of WCS this study had the objectives to evaluate the nutritional effects of gamma irradiation at doses of 8, 10 and 12 kGy on changes in nutrient contents, anti-nutritional factors, in vitro digestibility and ruminal degradability. No significant differences were found in proximate analysis of nutrients between raw WCS and gamma irradiated one. Glycine and threonine contents significantly increased when the WCS was exposed to gamma ray as compared to untreated WCS (p<0.05). As for fatty acid composition, no significant differences were observed with the irradiation treatment. Free gossypol in WCS was decreased (p<0.05) by gamma irradiation treatment. Of the 3 different levels of gamma irradiation, a dose of 12 kGy was found to be the most effective in reducing free gossypol concentration. Results obtained from in situ experiment indicated that gamma irradiation at a dose of 10 kGy significantly (p<0.05) lowered rumen degradability of both dry matter and crude protein as compared with raw WCS. However, there were no significant differences in rapidly degradable and potentially degradable fractions of crude protein due to 10 kGy gamma irradiation. Overall, this study show that gamma irradiation at a dose of 10 kGy is the optimum condition for removing germination ability of WCS, and could improve nutritive value for the ruminant with respect to the decrease in both ruminal protein degradability and gossypol content of WCS.

Effects of Gamma Irradiation on Microbial Decontamination, Extraction Yields and Physiological Effectiveness of Korean Medicinal Plants (한방약재의 오염 미생물 살균, 추출율 및 생리효능에 대한 감마선 조사의 영향)

  • Yook, Hong-Sun;Cha, Bo-Sook;Jo, Sung-Kee;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
    • /
    • v.30 no.3
    • /
    • pp.581-589
    • /
    • 1998
  • Effects of gamma irradiation on hygienic quality, extraction yields and physiological effectiveness in twenty-one kinds of Korean medicinal plants were investigated. Gamma irradiation at $5{\sim}10\;kGy$ eliminated the microorganisms contaminated in Korean medicinal plants. The total extraction yield in fifteen kinds of Korean medicinal plants increased by $5{\sim}25%$ at 10 kGy gamma irradiation. The physiological effectiveness such as antioxidation, anticomplement functions, nitrite scavenging and electron donating abilities of medicinal plants exposed to 10 kGy gamma irradiation were not different from that of the nonirradiated control.

  • PDF

Effects of Ozone Treatment and Gamma Irradiation on the Microbial Decontamination and Physicochemical Properties of Red Pepper Powder (고춧가루의 오염미생물 제거 및 이화확적 특성에 관한 오존처리와 감마선 조사의 영향)

  • 이성희;이현자;변명우
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.26 no.3
    • /
    • pp.465-467
    • /
    • 1997
  • The comparative effects of ozone treatment and gamma irradiation on the sterilization, physicochemical properties and sensory quality of red pepper powder were investigated. As for the sterilization of microorganisms, 7.5~10 KGy of gamma irradiation completely eliminated the coliforms, yeast and molds, and total aerobic bacteria. On the other hand, ozone treatment failed to eliminate the highly contaminated microbial load, especially total aerobic bacteria. The physicochemical properties including capsaicin, capsanthin, browning, fatty acid compositions and sensory quality were not significantly changed by gamma irradiation up to 10 kGy, whereas ozone treatment caused significant changes in fatty acid compositions and destruction of natural pigments (p<0.05). The above results led us to conclude that gamma irradiation was more effective than ozone treatment for the sterilization and maintenance of physicochemical and sensory qualities of red pepper powders.

  • PDF

Improved Detection of ${\gamma}-Irradiated$ Vibrio vulnificus after Heat and Cold Shock Treatment by Using Ethidium Monoazide Real-time PCR

  • Lee, Jung-Lim;Levin, Robert E.
    • Food Science and Biotechnology
    • /
    • v.18 no.3
    • /
    • pp.788-792
    • /
    • 2009
  • Gamma $({\gamma})-irradiation$ can be used to control pathogens such as Vibrio vulnificus in seafood. The effects of irradiation on microbial cell populations (%) have been studied in order to develop detection methods for irradiated foods. The method used in this study was ethidium bromide monoazide (EMA) real-time polymerase chain reaction (PCR), using V. vulnificus specific primer, EMA, and $SYBR^{(R)}$ Green to discriminate between ${\gamma}-irradiated$ and non-irradiated cells. Confocal microscope examination showed that ${\gamma}-irradiation$ damaged portions of the cell membrane, allowing EMA to penetrate cells of irradidated V. vulnificus. ${\gamma}-Irradiation$ at 1.08 KGy resulted in log reduction ($-1.15{\pm}0.13$ log reduction) in genomic targets derived from EMA real-time PCR. The combination cold/heat shock resulted in the highest ($-1.74{\pm}0.1$ log reduction) discrimination of dead irradiated V. vulnificus by EMA real-time PCR.

Effect of the Gamma-Ray Irradiation on the Electric and Optical Properties of SrTiO3 Single Crystals

  • Lee, Y.S.;Lim, Junhwi;Kim, E.Y.;Bu, Sang Don
    • Journal of the Korean Physical Society
    • /
    • v.73 no.10
    • /
    • pp.1566-1570
    • /
    • 2018
  • We investigated the visible emission property of $SrTiO_3$ (STO) single crystals irradiated with gammy-ray (${\gamma}$-ray) at various total doses up to 900 kGy. The electric and optical absorption properties of the irradiated STO samples were hardly changed with the ${\gamma}$-ray irradiation, compared with those of un-irradiated STO. In contrast, the visible emission near 550 nm increased with the ${\gamma}$-ray dose increasing. While the development of the visible emission was indicative of the increase of oxygen vacancies inside STO by the ${\gamma}$-ray irradiation, the newly generated oxygen vacancies were not significantly harmful to the electric and optical properties of STO. We concluded that the STO single crystal should have a good tolerance against the damage by the ${\gamma}$-ray irradiation.

Assays of Residual Antibiotics after Treatment of γ-ray and UV Irradiation (감마선과 UV 조사에 의한 항생제 분해)

  • Shin, Ji-Hye;Nam, Ji-Hyun;Yu, Seungho;Lee, Myunjoo;Lee, Dong-Hun
    • Journal of Radiation Industry
    • /
    • v.4 no.1
    • /
    • pp.39-45
    • /
    • 2010
  • The pollution of antibiotics is a major cause of spreading antibiotics resistant bacteria in the environment. Applications of ozonation, UV, and ${\gamma}-ray$ irradiations have been introduced to remove antibiotics in the effluents from wastewater treatment system. In this study, we compared the chemical (HPLC) and biological (antimicrobial susceptibility test, AMS) assays in measuring of the concentrations of residual antibiotics after ${\gamma}-ray$ and UV irradiation. Most samples were degraded by ${\gamma}-ray$ irradiation (1~2 kGy). However, lincomycin and tetracycline were not degraded by UV irradiation. The concentration of residual antibiotics, that was treated with ${\gamma}-ray$ and UV irradiation, measuring by bioassay was similar to HPLC. The concentrations of ${\gamma}-ray$ irradiated cephradine measured by AMS test were 2 times higher than that of HPLC assay, indicating AMS test is more sensitive than HPLC assay. These results indicate that ${\gamma}-ray$ irradiation technique is more useful than UV irradiation, and biological assay is more useful to detect the antibiotics and toxic intermediates in antibiotics degradation.

The Combined Effect of Gamma Knife Irradiation and p53 Gene Transfection in Human Malignant Glioma Cell Lines

  • Kim, Jeong-Eun;Paek, Sun-Ha;Kim, Dong-Gyu;Chung, Hyun-Tai;Kim, Young-Yim;Jung, Hee-Won
    • Journal of Korean Neurosurgical Society
    • /
    • v.37 no.1
    • /
    • pp.48-53
    • /
    • 2005
  • Objective: The purpose of this study is to elucidate in vitro responses to combined gamma knife irradiation and p53 gene transfection on human malignant glioma cell lines. Methods: Two malignant human glioma cell lines, U87MG (p53-wild type) and U373MG (p53-mutant) were transfected with an adenoviral vector containing p53 (MOI of 50) before and after applying 20Gy of gamma irradiation. Various assessments were performed, including, cell viability by MTT assay; apoptosis by annexin assay; and cell cycle by flow cytometry, for the seven groups: mock, p53 only, gamma knife (GK) only, GK after LacZ, LacZ after GK, GK after p53, p53 after GK. Results: Cell survival decreased especially, in the subgroup transfected with p53 after gamma irradiation. Apoptosis tended to increase in p53 transfected U373 MG after gamma irradiation (apoptotic rate, 38.9%). The G2-M phase cell cycle arrest markedly increased by transfecting with p53, 48 hours after gamma knife irradiation in U373 MG (G2-M phase, 90.8%). Conclusion: These results suggest that the in vitro effects of combined gamma knife irradiation and p53 gene transfection is an augmentation of apoptosis and G2-M phase cell cycle arrest, which are more exaggerated in U373 MG with p53 transfection after gamma knife irradiation.