• Title, Summary, Keyword: gamma irradiation

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Immunostimulating Effects of Angelica Gigas by Radio-Protective (당귀의 방사선 방호에 의한 생체면역 증진 효과)

  • Kim Kyung-Yoon;Jeong Hyun-Woo;Kim Gye-Yeop
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.5
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    • pp.1256-1260
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    • 2005
  • The purpose of this study was to investigate the effects of Angelica gigas on jejunal survival, endogenous spleen colony formation and jejunal crypt cells of mice irradiated with Gamma-ray irradiation. The subject of this study includes 42 mice which were divided into each 7 groups. Angelica gigas experiment groups were Angelica gigas + Gamma-ray(10Gy), Angelica gigas + Gamma-ray(3Gy), Angelica gigas. Gamma-ray(1 Gy), Gamma-ray control (10Gy), Gamma-ray control(3Gy), Gamma-ray control(1Gy), Normal groups. In the present study to evaluate the effect of Angelica gigas on jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells of mice Gamma-ray with each dose of Gamma-ray irradiation. The results of this study were as follows: In low-dose(1Gy) Gamma-ray radiation were treatment of Angelica gigas showed significantly increased(p<0.05) on the cell death apoptosis in crypt, intestine crypts survival of intestine after gamma-ray irradiation. High-dose(10Gy) Gamma-ray, treatment of Angelica gigas showed significantly increased(p<0.05) on the leukocyte. The above results suggest that Angelica gigas were immunostimulating effectively reduced Gamma-ray irradiation.

Quantitative Evaluation of Infectivity Change of Cryptosporidium parvum after Gamma Irradiation

  • Lee, Soo-Ung;Joung, Mi-Kyo;Nam, Tae-Kyoung;Park, Woo-Yoon;Yu, Jae-Ran
    • The Korean Journal of Parasitology
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    • v.47 no.1
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    • pp.7-11
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    • 2009
  • Cryptosporidium parvum is a well-known waterborne and opportunistic intracellular protozoan parasite that causes diarrheal illness. In this study, we quantitatively investigated reduction of the infectivity of C. parvum after gamma irradiation and repair of the infectivity during incubation time after irradiation. C. parvum oocysts were subjected to gamma irradiation at various doses (1, 5, 10, and 25 kGy), and the in vitro infectivity was measured by real-time PCR every day up to 7 days after irradiation. The in vitro infectivity of C. parvum on human ileocecal adenocarcinoma cells (HCT-8) was effectively reduced (> $2\;{\log}_{10}$) by irradiation at 10kGy or more. However, in the experiment to find out repair of the infectivity, recovery was not noted until day 7 post-incubation.

Effect of γ-Irradiation on the Physicochemical Properties of Zein Films

  • Lee, Sehee;Lee, Myoungsuk;Song, Kyung-Bin
    • Preventive Nutrition and Food Science
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    • v.8 no.4
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    • pp.343-348
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    • 2003
  • To elucidate the effect of gamma-irradiation on the physicochemical properties of zein films, the molecular and mechanical properties of the films were examined after irradiation at various irradiation doses. Gamma-irradiation of zein solutions caused the disruption of the ordered structure of the zein molecules, as well as degradation, cross-linking, and aggregation of the polypeptide chains based on an SDS-PAGE study. Gamma-irradiation increased the solubility of zein and decreased the viscosity due to cleavage of the polypeptide chains. Protein solubility of the zein films in urea/2-mercaptoethanol also increased with increasing irradiation doses. Alterations of the zein molecules by irradiation decreased water vapor per-meability by 12% and increased the elongation of zein films. However, mean tensile strength of the zein films was decreased by gamma-irradiation treatment. Measurement of Hunter color values indicated that irradiation caused a destructive effect on yellow pigments, resulting in a significant decrease in Hunter b values. The microstructure as observed by scanning electron microscopy showed that irradiated zein film had a smoother and glossier surface than the non-irradiated films.

Effect of Low Dose γ-Irradiation on the Fate and Cell Envelope of Bacillus cereus, Escherichia coli, and Salmonella Typhimurium

  • Mtenga, Adelard B.;Kassim, Neema;Lee, Won-Gyeong;Heo, Rok-Won;Shim, Won-Bo;Yoon, Yohan;Chung, Duck-Hwa
    • Food Science of Animal Resources
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    • v.31 no.6
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    • pp.843-850
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    • 2011
  • This study investigated the effect of low dose ${\gamma}$-irradiation on the damage of the cell envelopes and antibiotic sensitivity profiles of Bacillus cereus, Escherichia coli, and Salmonella Typhimurium. The bacteria suspension in tryptic soy broth was exposed to the ${\gamma}$-irradiation doses of 0, 1, 1.5, 3, and 5 kGy, and then stored at $0^{\circ}C$ for 24 h. A viability test, an antimicrobial sensitivity profile, and an electron microscopy were performed to observe the effects due to ${\gamma}$-irradiation treatment. B. cereus could survive the ${\gamma}$-irradiation up to 5 kGy while E. coli and S. Typhimurium were all deactivated at 1.5 kGy and 5 kGy, respectively. At 5 kGy, the cell count of B. cereus was significantly reduced, and the survived bacteria cells retained their important features. There were no significant changes observed in the antimicrobial sensitivity profile (p>0.05) for the recovered bacteria after irradiation treatment. Low dose ${\gamma}$-irradiation below 3 kGy was found to be insufficient to achieve decontamination of B. cereus and S. Typhimurium. Cell envelope damage and deactivation of different bacteria did not occur in the same manner; thus, deferent doses of ${\gamma}$-irradiation may be required for deactivation of different bacteria.

Studies on the Stability of Catapol Components, and Genotoxic Safety of ${\gamma}-Irradiated$ Rehmanniae Radix crude (감마선 조사(照射) 생지황(生地黃)의 Catapol 성분에 대한 안전성 및 유전독성학적 연구)

  • Choi, Ho-Young;Cho, Jung-Hee;Ahn, Duk-Kyun;Yook, Chang-Soo;Byun, Myung-Woo;Lee, Ju-Woon;Im, Moo-Hyeog;Kim, Do-Hoon;Kim, Jong-Wook
    • Korean Journal of Pharmacognosy
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    • v.36 no.2
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    • pp.75-80
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    • 2005
  • This study is concerning to identify the hygienic problems occurring in processing, storage, and distribution of Rehmanniae radix crude, and to investigate the possibility of application of safe and hygienic ${\gamma}-irradiation$ techniques. The results are as follows. To compare the contents of catapol, index compound of Rehmanniae Radix crude, between before and after ${\gamma}-irradiated$,0,2.5,5,7.5,10,12.5,15,20, and 30 kGy of ${\gamma}-irradiation$ was applied to standard catapol and Rehmanniae radix crude. The contents of catapol in standard material and Rehmanniae radix crude were decreased with the increase of ${\gamma}-irradiation$ level. However, active components of Rehmanniae Radix crude were not changed with ${\gamma}-irradiation$. These results indicate that active components of Rehmanniae Radix crude were preserved after ${\gamma}-irradiation$ and those of Rehmanniae Radix crude did not showed significant change after irradiation of ${\gamma}-ray$ up to 20 kGy.

Immunostimulating Effects of Acanthopanax in mice following Gamma-ray irradiation (방사선 조사 마우스에서 오가피의 면역활성 효과)

  • Kim, Gye-Yeop;Jeong, Hyun-Woo;Kim, Kyung-Yoon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.20 no.3
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    • pp.670-674
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    • 2006
  • This experimental study was carried out to investigate the immunostimulating effect of Acanthopanax, as Oriental rhizomata herbs, on jejunal survival, endogenous spleen colony formation, apoptosis in jejunal crypt cells and lipid peroxidation in the liver of mice following Gamma-ray irradiation. The subject of this study includes 72 mice which were divided into each 7 groups. Acanthopanax experiment groups were Acanthopanax. Gamma-ray(lOGy), Acanthopanax. Gamma-ray(3Gy), Acanthopnax. Gamma-ray(1Gy), Gamma-ray control(1OGy), Gamma-ray control(3Gy), Gamma-ray control(1Gy), Normal groups. The results of this study were as follows : Treatment with Acanthopanax showed significantly increased(p<0.05) on the cell death apoptosis in crypt, intestine crypts survival of intestine in mice following low-dose(1Gy) Gamma-ray radiation. And that significantly increased(p<0.05) on jejunal crypt survival and reduced(p<0.05) on lipid peroxidation in mice following high-dose(1OGy) Gamma-ray radiation. The above results suggest that Acathopanax were immunostimulating effectively reduced Gamma-ray irradiation.

Comparison of Skin Injury Induced by β- and γ-irradiation in the Minipig Model

  • Kim, Joong-Sun;Jang, Hyosun;Bae, Min-Ji;Shim, Sehwan;Jang, Won-Seok;Lee, Sun-Joo;Park, Sunhoo;Lee, Seung-Sook
    • Journal of Radiation Protection and Research
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    • v.42 no.4
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    • pp.189-196
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    • 2017
  • Background: The effects of radiation on tissues vary depending on the radiation type. In this study, a minipig model was used to compare the effects of ${\beta}$-rays from $^{166}Ho$ and ${\gamma}$-rays from $^{60}Co$ on the skin. Materials and Methods: In this study, the detrimental effects of ${\beta}$- and ${\gamma}$-irradiation on the skin were assessed in minipigs. The histopathological changes in the skin from 1 to 12 weeks after exposure to 50 Gy of either ${\beta}$- (using $^{166}Ho$ patches) or ${\gamma}$- (using $^{60}Co$) irradiation were assessed. Results and Discussion: The skin irradiated by ${\beta}$-rays was shown to exhibit more severe skin injury than that irradiated by ${\gamma}$-rays at 1-3 weeks post-exposure; however, while the skin lesions caused by ${\beta}$-rays recovered after 8 weeks, the ${\gamma}$-irradiated skin lesions were not repaired after this time. The observed histopathological changes corresponded with gross appearance scores. Seven days post-irradiation, apoptotic cells in the basal layer were detected more frequently in ${\beta}$-irradiated skin than in ${\gamma}$-irradiated skin. The basal cell density and skin thickness gradually decreased until 4 weeks after ${\gamma}$- and ${\beta}$- irradiation. In ${\beta}$-irradiated skin lesions, and the density and thickness increased sharply back to control levels by 6-9 weeks. However, this was not the case in ${\gamma}$-irradiated skin lesions. In ${\gamma}$-irradiated skin, cyclooxygenase-2 (COX-2) was shown to be expressed in the epidermis, endothelial cells of vessels, and fibroblasts, while ${\beta}$-irradiated lesions exhibited COX-2 expression that was mostly limited to the epidermis. Conclusion: In this study, ${\beta}$-rays were shown to induce more severe skin injury than ${\gamma}$-rays; however, the ${\beta}$-rays-induced injury was largely repaired over time, while the ${\gamma}$-rays-induced injury was not repaired and instead progressed to necrosis. These findings reveal the differential effects of ${\gamma}$- and ${\beta}$-irradiation on skin and demonstrate the use of minipigs as a beneficial experimental model for studying irradiation-induced skin damage.

Gamma-Irradiation Provides Microbiological Protection While Maintaining Sensory Quality Change of Fresh Kale Juice During Storage

  • Kim, Jee-youn;Song, Kyung-Bin
    • Preventive Nutrition and Food Science
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    • v.8 no.1
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    • pp.89-92
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    • 2003
  • The effect of gamma-irradiation on microbiological growth in kale juice during storage was studied. Fresh kale juice was prepared and irradiated at 0, 1, 3, 5, 7, 10, and 15 kGy. D values for total bacteria, yeast and mold, Salmonella, E. coli, and Pseudomonas were 3.6, 4.0, 3.2, 1.4, and 1.6 kGy, respectively. E. coli and Pseudomonas were eliminated completely at 5 and 7 kGy, respectively. Gamma-irradiation also reduced total viable bacteria during storage. Therefore, these results indicate gamma-irradiation can prevent microbial spoilage of fresh kale juice by inactivating pathogenic microorganisms.

Studies on the Storage Characteristics of Angelica dahuricae Radix, Glehnia littoralis Radix Treated with Gamma-irradiation (감마선 처리에 의한 방풍, 백지의 저장성 연구)

  • Kim, Soo-Min;Kim, Eun-Ju
    • The Korea Journal of Herbology
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    • v.24 no.1
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    • pp.1-7
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    • 2009
  • Objectives : The purpose of this study is to investigate on storage characteristics of Herbal materials treated with Gamma-irradiation(10 KGy). Methods : This experiments were carried out by field survey and storage characteristics were carried out by physicochemical determination. Results : Antimicrobial activity of oriental medicine materials(Angelica dahurica Radix, Glehnia littoralis Radix) were examined, together with investigation of effects on gamma-irradiation. Oriental medicine materials contaminated in microbial cell were tend to be pinhole in packaging materials(polypropylene) by bug and Larva. At the same time, PVDC (polyvinylldichloride) package also was founded in bug and Larva by microbial contamination during storage in room temperature. Conclusions : In conclusion, it is very desirable to dose gamma-irradiation(10 KGy) in these oriental medicine materials in order to prevent microbial activity.

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Estimation of nuclear heating by delayed gamma rays from radioactive structural materials of HANARO

  • Noh, Tae-yang;Park, Byung-Gun;Kim, Myong-Seop
    • Nuclear Engineering and Technology
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    • v.50 no.3
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    • pp.446-452
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    • 2018
  • To improve the accuracy and safety of irradiation tests in High flux Advanced Neutron Application ReactOr (HANARO), the nuclear energy deposition rate, which is called nuclear heating, was estimated for an irradiation capsule with an iridium sample in the irradiation hole in order. The gamma rays emitted from the radioisotopes (RIs) of the structural materials such as flow tubes of fuel assemblies and heavy water reflector tank were considered as radiation source. Using the ORIGEN2.1 code, emission rates of delayed gamma rays were calculated in consideration of the activation procedure for 8 years and 2 months of HANARO operation. Calculated emission rates were used as a source term of delayed gamma rays in the MCNP6 code. By using the MCNP code, the nuclear heating rates of the irradiation capsules in the inner core, outer core, and heavy water reflector tank were estimated. Calculated nuclear heating in the inner core, outer core, and heavy water reflector tank were 200-260 mW, 80-100 mW, and 10 mW, respectively.