• Title, Summary, Keyword: gamma-irradiation

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Genetic Variation Analysis of Arabidopsis (Arabidopsis thaliana L.) Plants Induced by Acute and Chronic Gamma Irradiation (감마선 완·급조사에 따른 애기장대의 유전적 유연관계 분석)

  • Goh, Eun Jeong;Kim, Jin-Baek;Ha, Bo-Keun;Kim, Sang Hoon;Kang, Si-Yong;Kim, Dong Sub
    • Journal of Radiation Industry
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    • v.5 no.4
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    • pp.347-352
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    • 2011
  • In order to identify the genetic relationship analysis by acute and chronic gamma irradiation, Arabidopsis (Arabidopsis thaliana L.) were irradiated with 200 Gy of gamma-rays using gamma-irradiator (3,000 Ci; Nordion, Canada) and gamma-phytotron (400 Ci; Nordion, Canada) for acute and chronic irradiation, respectively. Genetic relationship among two acute gamma-irradiated plants (A1 and A24) and three chronic gamma-irradiated plants (C1W, C2W, C3W) were analyzed using the amplified fragment length polymorphism (AFLP) technique compared with each non-irradiated plant. A total of 28 EcoRI and MseI primer combinations were used to screen 8 treatments by the ABI3130 capillary electrophoresis system. Amplified products by 28 primer sets showed 1,679 bands with an average of 51 bands per primer combination. Out of the total bands scored, 1,164 fragments were polymorphic bands, with different alleles existing among the treatments. The cluster analysis was performed using the UPGMA (Unweighted Pair Group Method using Arithmetic) in the computer program NTSYS-pc. In clustery analysis, acute gamma-irradiation showed higher genetic variation compared with chronic gamma-irradiation.

Development of a Stereotactic Device for Gamma Knife Irradiation of Small Animals

  • Chung, Hyun-Tai;Chung, Young-Seob;Kim, Dong-Gyu;Paek, Sun-Ha;Cho, Keun-Tae
    • Journal of Korean Neurosurgical Society
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    • v.43 no.1
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    • pp.26-30
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    • 2008
  • Objective : The authors developed a stereotactic device for irradiation of small animals with Leksell Gamma Knife Model C. Development and verification procedures were described in this article. Methods : The device was designed to satisfy three requirements. The mechanical accuracy in positioning was to be managed within 0.5 mm. The strength of the device and structure were to be compromised to provide enough strength to hold a small animal during irradiation and to interfere the gamma ray beam as little as possible. The device was to be used in combination with the Leksell G-$frame^{(R)}$ and $KOPF^{(R)}$ rat adaptor. The irradiation point was determined by separate imaging sequences such as plain X-ray images. Results : The absolute dose rate with the device in a Leksell Gamma Knife was 3.7% less than the value calculated from Leksell Gamma $Plan^{(R)}$. The dose distributions measured with $GAFCHROMIC^{(R)}$ MD-55 film corresponded to those of Leksell Gamma $Plan^{(R)}$ within acceptable range. The device was used in a series of rat experiments with a 4 mm helmet of Leksell Gamma Knife. Conclusion : A stereotactic device for irradiation of small animals with Leksell Gamma Knife Model C has been developed so that it fulfilled above requirements. Absorbed dose and dose distribution at the center of a Gamma Knife helmet are in acceptable ranges. The device provides enough accuracy for stereotactic irradiation with acceptable practicality.

Effects of Combined Treatment of Gamma Irradiation and Addition of Rosemary Extract Powder on Ready-to-Eat Hamburger Steaks: II. Improvement in Quality (감마선 조사와 로즈마리 추출분말 병용처리가 즉석 햄버거 스테이크에 미치는 영향. II. 품질 개선 효과)

  • 박경숙;김종군;이주운;오상희;이유석;김장호;김재훈;김왕근;변명우
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.4
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    • pp.694-699
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    • 2004
  • To evaluate combined effect of gamma irradiation and rosemary extract powder, hamburger steak was treated with 200 or 500 ppm of rosemary extract powder, or 200 ppm of butylated hydroxyanisole (BHA), gamma irradiation at the absorbed doses of 5, 10 and 20 k㏉, and stored at 5$^{\circ}C$ for 3 months. The content of TBARS of irradiated ready-to-eat hamburger steak after gamma irradiation was in order of : Control>Rosemary 200>BHA>Rosemary 500. Gamma irradiation accelerated lipid oxidation and decreased sensory qualify. However, the combined treatment of the addition of rosemary extract powder and irradiation was helpful for retarding the oxidation process and improving tie sensory characteristics during storage. Gamma irradiation decreased hardness and increased redness (a value) of ready-to-eat hamburger steak.

Effects of Gamma Irradiation on the Physicochemical Properties of Rice Flour Porridge (방사선 조사한 쌀가루로 만든 죽의 이화학적 특성)

  • Yang, Yun-Hyoung;Kim, Min-Hee;Kwon, Oh-Yoon;Lee, Kun-Jong;Park, Soo-Cheon;Lee, Ju-Woon;Byun, Myung-Woo;Kim, Mee-Ree
    • Korean journal of food and cookery science
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    • v.23 no.6
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    • pp.961-967
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    • 2007
  • The aim of this present study was to evaluate the effects of gamma-irradiation on the microbial and physicochemical characteristics of the rice flour porridge. The viscosity of the gamma-irradiated rice flour porridge was decreased as compared to that of the control. The soluble solid and reducing sugar content of the rice flour porridge was increased according to the gamma irradiation dose, while the blue value was decreased. From the results of a DSC curve, it was suggested that gamma irradiation delayed the retrogradation of the cooked rice flour porridge. The sensory score for overall acceptance and the chewing times for swallowing of the porridge, decreased with gamma irradiation doses above 3 kGy. Based on these results gamma irradiation may significantly enhance the swallowability of rice porridge, especially for elderly or infant subjects who have decreased mastication. However, more research is needed to improve the sensory qualities for the industrial application.

Effect of Rutin on Adhesion Molecules Expression and NO Production Induced by $\gamma$-irradiation in Human Endothelial cells

  • Son, Eun-Wha;Lee, Kang-Ro;Rhee, Dong-Kwon;Pyo, Suh-Kneung
    • Biomolecules & Therapeutics
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    • v.9 no.3
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    • pp.156-161
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    • 2001
  • Inflammation is a frequent radiation-induced following therapeutic irradiation. Treatment of human umbilical endothelial cells (HUVEC) with ${\gamma}$-irradiation (${\gamma}$IR) induces the expression of adhesion proteins such as intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin. Since the upregulation of these proteins on endothelial cell surface has been known to be associated with inflammation, interfering with the expression of adhesion molecules is an important therapeutic target. In the present study, we demonstrate that bioflavonoid rutin inhibits ${\gamma}$IR induced expression of ICAM-1, VCAM-1, and E-selectin on HUVEC in a dose- and time dependent manner. Rutin also inhibited ${\gamma}$IR induced production of NO. These data suggest that rutin has therapeutic potential for the treatment of various inflammatory disorder associated with an increase of endothelial leukocyte adhesion molecules.

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Effects of Cobalt-60 γ Irradiation on the Growth of Rabbits (Cobalt-60 gamma 선(線) 조사(照射)가 가토(家兎)의 성장(成長)에 미치는 영향(影響))

  • Sung, Jai Ki
    • Korean Journal of Veterinary Research
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    • v.12 no.1
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    • pp.1-5
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    • 1972
  • In oder to investigate the effects of cobalt-60 gamma ray irradiation on the body gains of rabbits aging approximately 50-day-old rabbits were subjected to single whole-body gamma irradiation externally. The results obtained were as follows: 1. There were significant differences of the body gains between control and treated groups except the 100 rads gamma-rays exposure group. 2. There were no differences in body gains between male and female rabbits. 3. Generally, the relation between gamma irradiated doses and the growth rates of rabbits were inversly proportional. 4. The $LD_{50}$ of the rabbits was 600 rads in case of single external whole-body gamma irradiation.

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Allicin Reduces Adhesion Molecules and NO Production Induced by γ-irradiation in Human Endothelial Cells

  • Son, Eun-Wha;Cho, Chul-Koo;Pyo, Suhkneung
    • IMMUNE NETWORK
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    • v.2 no.1
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    • pp.6-11
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    • 2002
  • Background: Inflammation is a frequent reaction following therapeutic irradiation. Since the upregulation of adhesion molecules on endothelial cell surface is known to be associated with inflammation, the expression of adhesion molecules is an important therapeutic target. Methods: Treatment of human umbilical endothelial cells (HUVECs) with ${\gamma}$-irradiation (${\gamma}IR$) induces the expression of adhesion proteins such as intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin. Changes in the expression of these proteins on ${\gamma}$-irradiated HUVECs which had been treated previously with allicin were measured by ELISA. Results: In the present study, we demonstrate that allicin inhibits the ${\gamma}IR$ induced expression of ICAM-1, VCAM-1, and E-selectin on HUVEC in a dose-dependent manner. Allicin was also found to inhibit the ${\gamma}IR$ induced production of nitric oxide (NO). Conclusion: These data suggest that allicin has a therapeutic potential for the treatment of various inflammatory disorders associated with increase numbers of endothelial leukocyte adhesion molecules.

Comparison of Effects of Ultraviolet and $^{60}$ Co Gamma Ray Irradiation on Nylon 6 Mono-filaments

  • Ohtsuka, Mika;Suzuki, Yoshino;Sakai, Tetsuya;Netravali, Anil N.
    • Fibers and Polymers
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    • v.5 no.3
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    • pp.225-229
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    • 2004
  • The effect of UV and $^60{Co}$ gamma radiations on the physical and mechanical properties of nylon 6 mono-filaments with different draw ratios has been studied. Specimens were exposed to either up to 25 Mrad of gamma or up to 168 hrs of intense UV irradiation. The results show that nylon mono-filaments exposed to gamma rays, with much higher quantum energy than UV, undergo a larger extent of molecular chain scission. Higher irradiation dose also results in the production of insoluble, macroscopic three-dimensional cross-linked network structure. The amorphous regions with a lower density of cohesive energy (lower molecular orientation) show a higher extent of cross linking reaction whereas amorphous regions with a higher density of cohesive energy (higher orientation) show higher extent of chain scission reaction, irrespective of UV ray or gamma ray irradiation.

Morphological Study on the Effects of $^{60}Co$ ${\gamma}-irradiation$ on the Testis in the Chicken ($^{60}Co$ 감마선조사가 닭의 정소에 미치는 영향에 관한 연구)

  • Lee, Dong-Myoung
    • Journal of radiological science and technology
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    • v.13 no.2
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    • pp.51-51
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    • 1990
  • This study was undertaken to observe the effects of $^{60}Co\;{\gamma}-irradiation$ on the cell of spermatogenic epithelium in the testis of the chicken. 16-week-old chicken were provided as an experimental group and compared with control group. The experimental group was divided into a single irradiation (800, 1000, 1200 rads) and into three partial irradiation group (800/3, 1000/3, 1200/3 rads). The morphological changes of epithelial cell of the testis were observed by means of hematoxyline and eosin stain. Microstructure of spermatocyte and sperm was observed by means of semithin section of electron microscopic specimen. The results obstained are summerized as follows. 1. Spermatogonia and sertoli cells were found to be isolated from the basal membrane of seminiferous tubules as dose of $^{60}Co\;{\gamma}-irradiation$ was increased. 2. Spermatocytes of pachytene stage were seperated from the cytotplasmic process of sertoil cell in case of 1000 rads of $^{60}Co\;{\gamma}-irradiation$. 3. Normal arrangement of the cell of spermatogenic epithelium was found in control group and only the partial irradiation group of 800 rads. Vaculation in the seminiferous was pronounced in case of a single irradiation group of 800 rads, but the irradiation group of 1000 rads and 1200 rads were found to be damaged severely in both a single and a partial dose.

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Effect of Gamma Irradiation on the Improvement of Beef Tenderness (감마선 조사가 쇠고기의 연도개선에 미치는 효과)

  • Yook, Hong-Sun;Lee, Ju-Woon;Lee, Kyong-Haeng;Kim, Duk-Jin;Shin, Heuyn-Kil;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
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    • v.31 no.4
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    • pp.1005-1010
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    • 1999
  • To study the shortening of rigor mortis and changes in the morphological properties of gamma-irradiated pre-rigor bovine muscle (M). Sternomandibularis during post mortem, this experiment was performed with a test of shear force and the observation of the ultrastucture of raw muscle. The time elapsed until maximum shear force values was shortened by gamma irradiation, depending upon the dose. The release of rigor mortis started earlier than control (non-irradiated muscle). A shortening of the length of the Z-line and the maintenance of the sarcomere length by gamma irradiation was observed. The breakdown of the perimysium of muscle bundles was observed more in irradiated samples than in the control. In conclusion, it is considered that gamma irradiation on pre-rigor beef shortens aging-period, improves tenderness and enhances the beef quality.

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