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Secondary Structure and Phylogenetic Implications of ITS2 in the Genus Tricholoma

  • Suh, Seok-Jong;Kim, Jong-Guk
    • Journal of Microbiology and Biotechnology
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    • v.12 no.1
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    • pp.130-136
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    • 2002
  • The internal transcribed spacer (ITS) region in the genus Tricholoma was analyzed, including for its primary nucleotide sequence and secondary structural characterization. The secondary structures of the ITS2 region in the genus Tricholoma were identified for use in bioinformatic processes to study molecular evolution and compare secondary structures. Ten newly sequenced ITS regions were added to the analysis and submitted to the GenBank database. The resulting structure from a minimum energy algorithm indicated the four-domain model, as previously suggested by others. The conserved secondary structure of the ITS2 sequences of the genus Tricholoma exhibited certain unique features, including pyrimidine tracts in the loops of domain A and a complete structure containing four domains, with motifs identified in other ITS2 secondary structures. A phylogenetic tree was derived from sequence alignment based on the secondary structures. From the resulting maximum parsimonious tree, it was found that the species in the genus Tricholoma had evolved monophyletically and were composed of four groups, as supported by the bootstrapping values and pileus color.

A Brief Chronicle of the Genus Cordyceps Fr., the Oldest Valid Genus in Cordycipitaceae (Hypocreales, Ascomycota)

  • Shrestha, Bhushan;Tanaka, Eiji;Han, Jae-Gu;Oh, Junsang;Han, Sang-Kuk;Lee, Kang-Hyo;Sung, Gi-Ho
    • Mycobiology
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    • v.42 no.2
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    • pp.93-99
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    • 2014
  • The earliest pre-Linnaean fungal genera are briefly discussed here with special emphasis on the nomenclatural connection with the genus Cordyceps Fr. Since its valid publication under the basidiomycetous genus Clavaria Vaill. ex L. (Clavaria militaris L. Sp. Pl. 2:1182, 1753), the genus Cordyceps has undergone nomenclatural changes in the post-Linnaean era, but has stood firmly for approximately 200 years. Synonyms of Cordyceps were collected from different literature sources and analyzed based on the species they represent. True synonyms of Cordyceps Fr. were defined as genera that represented species of Cordyceps Fr. emend. G. H. Sung, J. M. Sung, Hywel-Jones & Spatafora. The most common synonyms of Cordyceps observed were Clavaria and Sphaeria Hall, reported in the 18th and in the first half of the 19th century, respectively. Cordyceps, the oldest genus in the Cordyceps s. s. clade of Cordycipitaceae, is the most preferred name under the "One Fungus = One Name" principle on priority bases.

ISOMORPHISM CLASSES OF HYPERELLIPTIC CURVES OF GENUS 2 OVER $F_{2_}{N}$ FOR EVEN n

  • Park, Chun-Soo;Rhee, Min-Surp
    • Journal of applied mathematics & informatics
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    • v.13 no.1_2
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    • pp.413-424
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    • 2003
  • L. H Encinas, A. J. Menezes and J. M. Masque in [3] proposed a classification of isomorphism classes of hyperelliptic curve of genus 2 over finite fields with characteristic different from 2 and 5. Y. Choie and D. Yun in [2] obtained the number of isomorphic classes of hyperelliptic curves of genus 2 over $F_{2-}$ using direct counting method. We have obtained isomorphism classes of hyperelliptic curves of genus 2 over $F_{2n}$ for odd n, represented by an equation of the form $y^2$ + $a_{5}$ y = $x^{5}$ + $a_{8}$ x + $a_{10}$ ( $a_{5}$ $\neq$0) [1]. In this paper we characterize hyperelliptic curves of genus 2 over $F_{2n}$ for even n, represented by an equation of the form $y^2$ + $a_{5}$ y = $x^{5}$ + $a_{5}$ x + $a_{10}$ ( $a_{5}$ $\neq$0).>0).

Survey of Garlic Virus Disease and phylogenetic Characterization of Garlic Viruses of the Genus Allexivirus Isolated in Korea

  • Koo, Bong-Jin;Kang, Sang-Cu;Chang, Moo-Ung
    • The Plant Pathology Journal
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    • v.18 no.5
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    • pp.237-243
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    • 2002
  • A survey of virus infection in garlic plants cultivated in Korea was conducted for three years. Most virus-infected garlic plants (Allium sativum) showed typical symptoms on the leaves such as yellow mosaic, stripes, and distortion. Through immunosorbent electron micro-scopy and RT-PCR analysis, the complex mixtures of viruses including garlic viruses of the genus Allerivirus, gaylic strain of Leek yellow stripe virus of the genus Potyvirus, and Garlic latent virus of the genus Carlavirus were identified in the virus-infected garlic plants. Among these viruses, Allexivirus was the most frequently detect-ed in the regions surveyed. Using sets of differential primers for Allexivirus genomes, two members of the genus were amplified and sequenced from the purified viruses. The deduced amino acid sequences for the coat proteins and the nucleic acid binding proteins of two viruses showed high homologies to Garlic virus A (CarV-A) and Garlic virus D (GarV-D) of Allekivirus. This is the first report of GarV-A and GarV-D in Korea. This suggests that Allexivirus in gavlic plants in Korea was mixed and varied. Phylogenetic analyses showed that the genus Allexivirus was diversi(ied by the processes of accumulation and evolution of viruses in garlic plants due to the long period of repeated vegetative propagation.

Diversity of Actinomycetes - Species, morphology and life cycle - (방선균의 다양성 -종류, 형태 및 생활환-)

  • 아끼라시마즈;김창진;유익동
    • Microbiology and Biotechnology Letters
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    • v.21 no.1
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    • pp.88-94
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    • 1993
  • Actinomycetes are the most diverse group of bacteria. in variety. morphology. and life cycle. Especially. the variety of genus and species of Actinomycetes is so diverse among other microorganisms that about 50 genus of Actinomycetes have been reported until now. Among them. Streptomyces genus is the most plentiful and over 220 species have been reported. The number of the genus of Actinomycetes will be expected to be fixed to about 55 genus and 250 Streptomyces species in the year 2000 thereabouts. Morphological and physiological characters of Actinomycetes varies with culture condition. and therefore diverse life cycles are also possible. In this paper. life cycles of the representative Actinomycetes, Nocardia, Streptomyces. Kitasatosporia. GeodermatoPhi/us. and Sporichthya. according to the condition of nutrient in the media were reviewed.

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Redescription of three trapanian nudibranchs (Nudibranchia, Goniodorididae) from Korea with a key to the species

  • Jung, Dae-Wui;Kil, Hyun Jong;Nam, Eunjung;Kim, Hyeonggeun;Kim, Chang-Bae
    • Journal of Species Research
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    • v.11 no.3
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    • pp.169-173
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    • 2022
  • Three species belonging to the genus Trapania Pruvot-Fol, 1931 are redescribed from Korea in this paper: Trapania euryeia Gosliner & Fahey, 2008, T. japonica (Baba, 1935), and T. toddi Rudman, 1987. Among these species, T. japonica is newly added to Korean fauna. The genus Trapania is characterized by a pair of extra-rhinophoral appendages on each side of the head, tentacular foot corners, a pair of extra-branchial appendages present around the gill, radula formula N×1.0.1. and consists of denticulated teeth, triaulic reproductive system, and minute spines on the armed penis. Herein, synonyms of the genus Trapania are summarized through a detailed literature review and the diagnostic characters of the genus Trapania are provided. Three species of the genus Trapania from Korea are distinguished from each other based on the color of extra-rhinoporal appendages and extra-branchial appendages, ground color, and distribution range of the brown markings on the dorsal surface. A taxonomic key to the genus Trapania in Korea is provided. In addition, the morphological characteristics of three trapanian nudibranchs in Korea are described and detailed photos of living animal are provided.

A Study on the Herbal Name and Four Qi(四性) of Genus Curcuma in the Korea Pharmacopoeia Ninth Edition ($\ll$대한약전$\gg$ 제9개정에서 Curcuma속(屬)의 한약명과 약성에 관한 연구)

  • Kim, In-Rak
    • The Korea Journal of Herbology
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    • v.24 no.3
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    • pp.21-28
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    • 2009
  • Objectives : The purpose of this study is to determine the herbal name and four Qi(四性) of genus curcuma in the Korea Pharmacopoeia ninth Edition. Methods : Compare with the name and four qi of genus curcuma from the Korea Pharmacopoeia ninth Edition and the other nation's Pharmacopoeia and main herbal medicine books. Results : 1. The Rhizoma of Curcuma longa is called Kanghwang in KP 9, but is called Ulgeum in the main herbal medicine book. 2. The Rhizoma of genus curcuma except Curcuma longa is called Achul in KP 9, but is called Kanghwang in the main herbal medicine book. 3. The root of genus curcuma is called Ulgeum in KP 9, but is called Achul in the main herbal medicine book. 4. The four qi of the Rhizoma and Radix of genus curcuma is recorded worm and cold in the Ch. p 2005, but is recorded cold and worm in the main herbal medicine book. Conclusions : Kanghwang, Achul, Ulgeum in the KP 9 is equivalent Ulgeum, Kanghwang, Achul in the main herbal medicine book. The four qi of Rhizoma is cold, Radix is warm.

Effects of the Proliferation of Beneficial and Harmful Enteric Bacteria after Intake of Soybean Fermentation (Zen) Produced by a Mixture of Lactobacilli and Saccharomyces (Lactobacilli와 Saccharomyces 혼합균주의 대두발효액(Zen) 섭취 후 장내 유익세균과 유해세균의 증식에 미친 영향)

  • Won, Ryu Seo;Lee, Hyung H.
    • Journal of Naturopathy
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    • v.8 no.1
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    • pp.1-10
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    • 2019
  • Purpose: The purpose of this study was to investigate the increase or decrease of important intestinal beneficial bacteria and inhibitory bacteria in 30 stools of clinical subjects after ingesting Zen fermentation broth as a mixed microbial fermentation solution for eight weeks. Methods: Intestinal bacteria were identified by PCR amplification using specific primers. Results: Bifidobacterium genus gi% of test group ingested Zen-fermented broth was 55.15% before and 70.1% after ingestion, so it was a significant difference (p<.009). Lactobacillus genus of the test group was 46.87% before and 60.91% after ingestion, it was a significant difference (p<.01). Clostridium genus of the test group was 85.64% before and 65.99% after ingestion. There was a significant difference (p<.017) as the pre-post-difference decreased to -19.65%. Bacteroides genus of the test group was 17.11% before and 20.22% after ingestion. There was a significant difference (p<.048) as the pre-post-difference increased to 3.11%. Prevotella genus of the test group was 14.01% before and 16.79% after ingestion, so it was not a significant difference. Conclusions: Intestinal bacteria increased the proliferation of beneficial bacteria and suppressed harmful bacteria in the intestines after ingesting the Zen-fermented broth of the mixed microorganism. The Zen fermentation broth evaluated as a beneficial drink for intestinal health.

2D GENUS TOPOLOGY OF 21-CM DIFFERENTIAL BRIGHTNESS TEMPERATURE DURING COSMIC REIONIZATION

  • Hong, Sungwook E.;Ahn, Kyungjin;Park, Changbom;Kim, Juhan;Iliev, Ilian T.;Mellema, Garrelt
    • Journal of The Korean Astronomical Society
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    • v.47 no.2
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    • pp.49-67
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    • 2014
  • A novel method to characterize the topology of the early-universe intergalactic medium during the epoch of cosmic reionization is presented. The 21-cm radiation background from high redshift is analyzed through calculation of the 2-dimensional (2D) genus. The radiative transfer of hydrogen- ionizing photons and ionization-rate equations are calculated in a suite of numerical simulations under various input parameters. The 2D genus is calculated from the mock 21-cm images of high-redshift universe. We construct the 2D genus curve by varying the threshold differential brightness temperature, and compare this to the 2D genus curve of the underlying density field. We find that (1) the 2D genus curve reflects the evolutionary track of cosmic reionization and (2) the 2D genus curve can discriminate between certain reionization scenarios and thus indirectly probe the properties of radiation-sources. Choosing the right beam shape of a radio antenna is found crucial for this analysis. Square Kilometre Array (SKA) is found to be a suitable apparatus for this analysis in terms of sensitivity, even though some deterioration of the data for this purpose is unavoidable under the planned size of the antenna core.

Separation of Wild Yeast from Intestine of Othius punctulatus and Microbiological Characteristics of Unrecorded Wild Yeast Strains in Korea (반날개과 곤충 장으로부터 야생 효모의 분리 및 국내 미기록 효모들의 균학적 특성)

  • Lee, Sang Eun;Han, Joo Hyun;Kim, Myung Kyum
    • The Korean Journal of Mycology
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    • v.48 no.3
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    • pp.229-235
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    • 2020
  • The goal of this study was to isolate and identify the wild yeast strains in intestine of Othius punctulatus collected in Cheongju-si, Chungcheongbuk-do, Korea and characterize their unrecored yeast strains. We isolated 16 wild yeast strains from 5 intestine samples from Othius punctulatus. The 16 wild yeast strains were included three strains in the genus Aureobasidium, four strains in the genus Cystofilobasidium, one strain in the genus Mrakia, three strains in the genus Naganishia, two strains in the genus Saitozyma, two strains in the genus Sampaiozyma and one strain in the genus Scheffersomyces. Among them Cystofilobasidium capitatum YP53, Cystofilobasidium capitatum YP71, Cystofilobasidium macerans YS620, Cystofilobasidium macerans YS622, Mrakia aquatica YP158 and Scheffersomyces stipitis YI5 were recorded for the first time in Korea. The microbiological characteristics and carbon assimilation of these previously unrecorded yeasts were investigated. Almost all of them were oval-shaped. And, glycerol, L-arabinose, xylitol, and inositol are not utilized.