• Title/Summary/Keyword: germination

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Effect of Temperature on Seed Germination of Korean Native Viola Species

  • Lee, Cheol-Hee;Hwang, Ju-Kwang
    • Korean Journal of Plant Resources
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    • v.19 no.6
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    • pp.700-705
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    • 2006
  • Present studies were performed to determine the physiology of seed germination in Viola species native to Korea. Twelve species, 1 variety and 1 form were collected, classified and used as materials: V. collina, V. blandaefomis, V. rosii, V. chaerophylloides, V. phalacrocarpa, V. patrinii, V. mandshurica, V. mandshurica for. albescence, V. seoulensis, V. yedoensis, V. keiskei, V. variegata, V. variegata var. chinensis, and V. verecunda. V. tricolor 'Helen Mount' was also used to compare wild with cultivated species. In order to investigate the effect of temperature on seed germination, seeds stored at $4{\pm}2^{\circ}C$ for 10 months or 4 years were incubated at 10, 15, 20, $25^{\circ}C$ under 16h illumination with 4 replicates per treatment. Seeds which had not germinated at $10^{\circ}C$ were transferred to $30^{\circ}C$ to assess the effect of temperature change in germination. Germination percent and the days of first, 40% and 80% germination were assessed. Capability of seed germination varied with taxon; Species belonging to subsection Patellares had high ability of germination, compared to species in the other subsections, and series Chinensis was the best among subsection Patellares. Species capable of high germination germinated in all temperatures with reasonably high germination rate, but the other species responded sensitively to temperature with different germination patterns. Higher the temperature, shorter the incubation time required for first, 40% and 80% of germination. Therefore, high temperature was effective in almost all species, not only for inducing high rate of germination but also the uniformity of germination. Temperature change from $10^{\circ}C\;to\;30^{\circ}C$ had a positive effect on seed germination.

Effect of NaCl on Germination of Artiplex gmelini and Pharagmites communis (갈대 및 가는갯능쟁이의 발아에 미치는 NaCl의 효과)

  • 김관수
    • Journal of Plant Biology
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    • v.28 no.3
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    • pp.253-259
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    • 1985
  • The results of the conducted experiment obtained basic data on seed germination for Phragmites communis and Atriplex gmelini were; Seed germination was not influenced till 0.5% NaCl, but at over 1.0% NaCl it dropped remarkably. The germination limit fro degree of NaCl was 2.0% in Phragmites communis. Atriplex gemelini was 2.5% and in accordance with the increase of the degree of NaCl. Germination speed showed a negative correlation being highly significant and the germination period lengthened. Compared with growing top plants, growth of roots was largely influenced by a high degree of NaCl. In accordance with the rise of temperature, the germination rate, and speed of both plants remarkably increased and the germination period was shortened. Ubride of Atriplex gmelini was germinated at the early days of picking but was not as the passing of the period. The seeds also did not germinated likewise Ubride. By a seedcoat breaking germination became 81%. During 20 min soaking treatment in conc H2SO4 seed germination possibility of 63% was known to be hard. Adequate soaking time in conc H2SO4 was 17.5 min.

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Effect of Air-Phase Germination with Anion Radiation and Water-Spraying on Germination Ratio, Sprout Growth, and GABA Contents of Germinated Brown Rice

  • Lim, K.T.;Chung, J.H.;Hong, J.H.;Kim, J.H.;Lee, E.T.;Im, A.L.
    • Agricultural and Biosystems Engineering
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    • v.7 no.1
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    • pp.42-47
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    • 2006
  • The objective of this study was to investigate the effects of air-phase germination with water-spraying and anion stimuli on germination ratio, sprout growth and ${\gamma}$-aminobutyric acid (GABA) of brown rice. Air-phase germination method with intermittent spraying water improved germination ratio and sprout growth by about 100% compared with the conventional water-soaking method. Anion radiation was applied during the germination process and improved the germination ratio, sprout growth and color quality of the germinated brown rice. Germination ratio and sprout growth were improved up to 9% with anion radiation, and its brightness was higher than brown rice germinated with no anion radiation. The air-phase germination with water-spraying improved the GABA content of germinated brown rice by about 8-9 times compared with that of brown rice.

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The Effects of Drying Conditions on the Germination Properties of Rapeseed (유채종자의 건조조건에 따른 발아특성)

  • Duc, Le Anh;Han, Jae-Woong
    • Journal of Biosystems Engineering
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    • v.34 no.1
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    • pp.30-36
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    • 2009
  • This study was performed to determine the effect of drying conditions on the germination properties of rapeseed after seeds were dried under different drying conditions: $40^{\circ}C$, $50^{\circ}C$, or $60^{\circ}C$ in combination with 30%, 45%, or 60% relative humidity. As analytic results, drying conditions had significant effects (P-value < 0.001) and drying temperature was considered as the main factor on the germination properties of rapeseed. When drying temperature increased or relative humidity decreased, the vigor rate and germination rate decreased, the median germination time increased. The maximum values of vigor rate and germination rate were 90% and 95.44%, and their minimum values were 60.17 and 75%, respectively. To ensure the standard germination rate of 85%. The appropriate drying zone was determined and the drying temperature should be less than $51.0^{\circ}C$, $54.5^{\circ}C$ and $58.7^{\circ}C$ at 30%, 45% and 60% RH, respectively. The values for median germination time varied from 2 to 4 days. The predicted models of germination rate, vigor rate, and median germination time were determined.

The Effect of Light, Temperature and GA Soaking Treatment on Seed Germination of Cool Season Turfgrasses (광, 온도 및 GA 침지처리가 한지형 잔디의 종자발아에 미치는 영향)

  • Hyeon, Sang-Min;Kang, Hoon;So, In-Sup;Kim, Dong-Il
    • Asian Journal of Turfgrass Science
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    • v.9 no.3
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    • pp.253-260
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    • 1995
  • This study is aimed to understand the effects of temperature, light and GA soaking treatment on the seed germination of perennial ryegrass 'Dandy', creeping hentgrass 'Penlinks', tall fescue 'Arid' and Kentucky hluegrass 'Nasaw'. These experiments are carried in vitro, and their results obtained are summarized as the followings ; The optimum temperature for seed germination of perennial ryegrass 'Dandy' was found to he ranged at 20~30$^{\circ}C$, and the optimum temperature for germination of creeping bentgrass 'Penlinks', tall fescue 'Arid' and kentucky bluegrass 'Nasaw' seeds was found to be ranged at 20~25$^{\circ}C$, whereas the germination percentage under the below or above temperature of this range decreased. The germination of perennial ryegrass 'Dandy' and tall fescue 'Arid' seeds showed no effect on light, and while the germination percentage of creeping hentgrass 'Penlinks' increased more highly under the dark condition than under the light condition. The germination percentage decreased according to the higher temperature. The germination of Kentucky bluegrass 'Nasaw' seeds increased more highly under the dark condition than under the light condition at 20~25$^{\circ}C$, whereas the germination percentage under the dark condition at 15$^{\circ}C$ and 30$^{\circ}C$ decreased. In case of GA soaking treatment of seeds, all the cool season turfgrasses tested showed their own promoting effects on seed germination. The early germination of tall fescue 'Arid' was good without the soaking times. Creeping bentgrass 'Penlinks' and perennial ryegrass 'Dandy' had re-spectively the highest germination percentage at 10min and 1hr soaking treatment, and the longer the soaking time, the lesser the germination percentage. Kentucky bluegrass 'Nasaw' obtained the best results at 24has soaking treatment, and the longer the soaking time, the better the ger-mination percentage.

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Comparison of Seed Germinating Vigor, Germination Speed and Germination Peak in Kentucky Bluegrass Cultivars under Different Germination Conditions (발아환경에 따른 켄터키 블루그래스의 종자 발아력, 발아세 및 발아피크 비교)

  • Kim, Kyoung-Nam
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.18 no.3
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    • pp.23-38
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    • 2015
  • The study was initiated with Kentucky bluegrass (KB, Poa pratensis L.) to investigate germination and early establishment characteristics of new cultivars for a practical application to turfgrass establishment such as parks, athletic field and golf course etc. Fifteen cultivars were evaluated in different experiments. An alternative condition for a KB germination test required by International Seed Testing Association (ISTA) was applied in Experiment I, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA conditions). Experiment II was conducted under a room temperature condition of 5 to $25^{\circ}C$ (natural conditions). Seed germinating vigor, germination speed and germination peak time were measured in both experiments. Significant differences were observed in seed germinating vigor, germination speed and germination peak time. Seed germinating vigor was variable with different environments and cultivars. It was 75.25 to 89.50% under ISTA conditions and 75.75 to 90.25% under natural conditions. There were considerable variations in early germination characteristics among KB cultivars according to different environments. Early germination characteristics showed that all cultivars were 3 to 5 days faster in germination under ISTA conditions, when compared with natural conditions. The germination speed, measured as days to seed germination of 50% to 80%, was much faster with 'Midnight II', 'Excursion' and 'Midnight' under ISTA conditions. But it was faster with 'Midnight II', 'Excursion' and 'Odyssey' under natural conditions. Differences was also observed in germination peak time with cultivars and growing conditions. It ranged 5.94 to 14.88 days under ISTA conditions and 4.71 to 13.06 days under natural conditions. Regardless of the environment conditions, the shortest cultivars were 'Odyssey' and 'Midnight II'. The longest ones were 'Nuglade' under ISTA condition and 'Moonlight' under natural conditions. Considering germinating vigor, germination speed and germination peak time, 'Midnight II', 'Excursion', 'Midnight', and 'Odyssey' were regarded as excellent cultivars under ISTA conditions in terms of early establishment characteristics, while 'Midnight II', 'Excursion', 'Odyssey', and 'Courtyard' under natural conditions. These results suggest that an intensive germination test be needed prior to planting, for investigating the early germinating vigor, germination speed and germination peak time. Also, a proper cultivar selection and comprehensive site analysis for the growing environmental conditions should be done before turfgrass establishment such as golf course construction.

Environmental factors influencing on tuber germination in scirpus maritimus l. (매자기 槐莖의 發芽에 미치는 環境 要因들)

  • Yang, Hae-Kyeng;Kim, Ok-Kyung
    • The Korean Journal of Ecology
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    • v.15 no.2
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    • pp.127-135
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    • 1992
  • The effects of nacl concentration, temperatura, light and growth regulator(GA3, kinetin) on the tuber germination of s. maritimus were investigated. The germination percentage increased with decreasing nacl, showing salt tolerance through time, and with increasing temperature untile 20~30oC light / dark (12/12 hr) of optimal temperature. The multiplication of nacl and temperature on germination percentage and velocity, increased significantly in higher temperature than lower temperature in saline. The germination percentage showed high value in dark condition than in light condition which is the charracteristics of underground organ. and ga3 act as germination stimulator to overcome the inhibitory effect of nacl. The effect of ga3 showed significant differances on tubers of s. maritimus of germination but that of kinetin had a litter sffects on germination. Factors of nacl and temperature interacted significantly and the effects of nacl on germination percentage and velocity depended on temperature condition.

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Studies on Establishment of Oversown Pasture Seed I. Effects of coating materials and minerals on germination (겉뿌린 목초종자의 정착에 관한 연구 I. 각종 증량재 및 미량광물질의 종자피복이 발아에 미치는 영향)

  • 이효원;정병용;김희경
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.7 no.2
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    • pp.113-119
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    • 1987
  • This experiment was carried out in laboratory to find out the effect of coating materials, minerals and insecticides on germination of pasture seed. Seed coating was made by specially made machine and seed germination in petridishes was also determined from Sep. 1986 to May 1987. The results were summarized as follows; 1. Coating seed gave bad germination compared with the control. There were significant difference between treatment, coating materials and adhesives in white and red clover while Phleum pratense germination was improved by coating materials. 2. In mineral coating treatments, on Mg alone or Mg mixed treatment, germination was deepressed. Generally germination was lowed by mineral treatment, but the difference was small. 3. Uncoating treatment with insecticide was superior to coating treatment in terms of germination. Among the insecticide Ballen gave more serious effect to seed germination.

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Environmental Factors Affecting Conidial Germination of Persimmon Leaf Blight on Sweet Persimmon Tree (단감나무 둥근갈색무늬병균(Pestalotiopsis theae)의 분생포자 발아에 미치는 환경요인)

  • 장태현;임태헌;정봉구
    • Korean Journal Plant Pathology
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    • v.14 no.2
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    • pp.120-124
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    • 1998
  • The conidial germination of Pestaotiopsis theae was occurred in cells attched with pedicels, the inferior cell of conidium. The cells were swollen like a ball, and then germinated in 4 hours under favorable conditions. Generally, it was considered that fifty percent of whole conidia was germinated in the range 6~12 hours. The optimum temperature and pH for conidial germination of P. theae (SP-3) causing leaf blight on sweet persimmon was $25^{\circ}C$ and pH 5, respectively. Conidial germination rate was higher than 90% at 100% relative humidity, but never germinated at a relative humidity lower than 88.5%. Conidial germination was highest at the concentration of 1~4$\times$104 conidia/ml. In case of cultural media, the conidial germination was higher than 90% on PDA, PSA, OME and Leonian agar. There was no relationship between light and conidial germination. It was concluded that the key environmental factors affecting conidial germination of the fungus (SP-3) were temperature and moisture.

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Removal Effect of Biostone and Green Tea on the Heavy Metal Toxicity during Seed Germination of Arabidopsis thaliana (애기장대의 종자 발아에 미치는 맥반석과 녹차의 중금속 제거 효과)

  • 박종범
    • Journal of Environmental Science International
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    • v.12 no.12
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    • pp.1303-1308
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    • 2003
  • This experiment was carried out to investigate the effects of heavy metals (cadmium, chromium, copper and lead) on the seed germination of Arabidopsis thaliana, and examinated the removal effects of biostone and green tea on the heavy metal toxicity. Cadmium and chromium among the four heavy metals had no effect on the seed germination even in the concentration fifty times higher than in the official standard concentration of pollutant exhaust notified by the Ministry of Environment. However, seeds were not germinated in the concentration of copper ten times higher and in the concentration of lead fifty times higher than the official standard concentration. When seeds were sown in the solutions of lead (15, 20, 25 and 30 mg/L) and copper(15 and 20 mg/L), the seed germination rates were 0% and less than 10%, respectively. However, when biostone(3 g/30 $m\ell$) was added, the seed germination rate was 100% in all the concentrations. The germination rate was 100% in distilled water and copper solution (5 mg/L). However, green tea (0.2 g/30 $m\ell$) was added, the seed germination rate was 0% in both. The results show that cadmiun and chromium had no effect on the seed germination, but lead and copper decreased the rate of seed germination of Arabidopsis thaliana, Biostone removed heavy metal toxicity, but green tea did not removed heavy metal toxicity during germination.