• 한국작물학회지
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• 제45권2호
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• pp.108-111
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• 2000

Osmoconditioning and hardening effects for the seed germination of normal and naturally aged rice seeds were studied through analyzing the total sugars and $\alpha$-amylase activity. The normal seeds which used to have high germination rate accelerated germination with the osmoconditioning at the suboptimal temperature of 17$^{\circ}C$. On the other hand, the aged seeds did not affect germination rate at $25^{\circ}C$, while increased germination rate and accelerated germination a little at 17$^{\circ}C$, Hardening of aged seeds increased germination rate by 10-15% compared with control seeds at both 17 and $25^{\circ}C$ and accelerated germination. Total sugar content and $\alpha$-amylase activity of normal seeds were higher than did aged seeds. The aged seeds with treatment of osmoconditioning and hardening increased total sugar content and $\alpha$--amylase activity, but hardening was more effective than osmoconditioning. The $\alpha$--amylase activity was positively correlated with the total sugar content and germination rate.

• Journal of Ecology and Environment
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• 제33권3호
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• pp.187-193
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• 2010

Seed germination is a biological process that is affected by a variety of genetic and environmental factors. The cardinal temperature and thermal time are required for germination. The principal objective of this study was to identify and characterize variations in the base, optimum, and maximum germination temperatures of Haloxylon aphyllum L. from two seed sources, in order to establish models for use in predicting seeding dates. Mature H. aphyllum seeds were germinated at temperatures between 5 and $35^{\circ}C$. The germination behavior of H. aphyllum seeds to different temperature regimens in light was evaluated over a temperature range of $5-35^{\circ}C$ at intervals of $5^{\circ}C$. The rate of germination increased between base and optimum thermal conditions, and decreased between optimum and maximum thermal conditions; the germination rate varied in a linear fashion at both sub-optimal and supra-optimal temperatures. The linear regression fit the range of germination rates at $5^{\circ}C$ to $25^{\circ}C$ and $25^{\circ}C$ to $30^{\circ}C$, and thus the base temperature, optimum temperature, and maximum temperature for the germination of H. aphyllum were measured to be $0.6^{\circ}C$, $25.69^{\circ}C$, $37.90^{\circ}C$, and $1.76^{\circ}C$, $21.56^{\circ}C$, $37.90^{\circ}C$ for Qom and the Fars dune desert respectively.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.212-212
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• 2017

This experiments were conducted to evaluate the influence of Chlorella culture solution on seed germination of perennial ryegrass seeds. Chlorella are known to contain different bioactive compounds. In present research work, Chlorella culture solution using liquid manure as medium have been used to study their effects on germination and root length. The study conducted a germination experiment in petri-dishes. Four treatments were compared: non-treated control treated with distilled water, Chlorella culture solution and Chlorella culture filtrate, and liquid manure. The germination percentage of perennial ryegrass seeds was highest in the Chlorella culture solution treatment. Days required for 50, 70% seed germination was the fast in Chlorella culture solution and the Chlorella culture filtrate treatment. Root length of perennial ryegrass seeds was long by 1~2cm in the Chlorella culture solution compared with no treated control. The germination index of perennial ryegrass seeds was high by 180~202% in the Chlorella culture solution treatment compared to no treatment. Chlorella culture solution and the Chlorella culture filtrate have shown stimulatory effects in germination and development of root. Use of Chlorella culture solution and the Chlorella culture filtrate can be recommended to farmers as a ecofriendly practice for better germination and growth. Present research work reveals that Chlorella contain certain growth promoting substances which enhances seed germination.

• 한국작물학회지
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• 제45권1호
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• pp.55-58
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• 2000

Germination of sweet and super sweet corn is lower than normal corn due to the higher sugar and lower starch contents of kernels. Sweet corn seeds are easily deteriorated in the field under the unfavorable condition, therefore it is important to identify the optimal harvesting time for seed production. This trial was conducted to investigate the responses of germination percentage of shrunken-2(sh2), brittle(bt), sugary(su), and sugary enhancer(se) hybrids in relation to harvesting dates. Eight hybrids of four different gene sweet corns were harvested at 15, 20, 25, 30, 35, 40, 45, and 50 days after silking(DAS). Germination test was performed using paper towel method. Mean germination percentages across eight hybrids showed the highest value at 45 DAS. There were significant differences among genes and within gene for germination. Shrunken-2 hybrid Mecca was higher than su hybrids for germination, indicating that sh2 would not be poorer than su Late harvesting beyond the optimal harvesting date might not be desirable because of more lodging and ear rots. Theoretical optimal harvesting date estimated from the regression equation was 40.9 DAS, however, practical date for harvesting would be a few days later than the estimated date if seedling vigor might be considered. Kernel dry weight per ear showed similar response to germination. Regression equation showed the highest kernel dry weight at 40.7 DAS. Significant correlations between kernel dry weight and germination were observed, impling that kernel dry matter accumulation would be an important factor for germination.

• Environmental Analysis Health and Toxicology
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• 제17권1호
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• pp.29-35
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• 2002

The test of germination has been used as a good indicator to assess the toxicity of chemicals to plant. This study was carried out to investigate the effect of PAHs, anthracene and phenanthrene, on germination ratio, germination index and germination time of barley, tall fescue and alfafa. Anthracene and phenathrene induced to decrease the seedling length and increase the germination time of the experimental plants. But they did not affect the maximum germination ratio. While it was not correlated between seedling length and final germination ratio, it was shown a close relationship between seedling length and mean germination time of the plants. These results suggested that the mean of germination time and seedling growth could be useful to exmine the phyto -toxicological effect of PAHs. The mixture of anthracene and phenanthrene was examined to investigate the combined effect on seedling length; the mixture induced the reduction of seedling. This was meant that the mixture toxicity of PAHs might be not shown in the sum of each chemical toxicity.

• 원예과학기술지
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• 제34권3호
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• pp.363-371
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• 2016

Tabulis [Astilboides tabularis (Hemsl.) Engl.] has the potential for becoming a useful horticultural crop. This study was conducted to classify the seed dormancy types and to identify the germination conditions that improve the horticultural usefulness of this plant. We found that A. tabularis (Hemsl.) Engl. produced undifferentiated embryos and had a low germination rate of under 50%. In addition, water submersion led to moisture absorption. Therefore, A. tabularis seeds were designated as morphologically dormant. The germination rate was highest (86.3%) at $30^{\circ}C$ under light conditions. Higher temperatures generally led to an acceleration of the germination process, regardless of light condition. To improve the germination rate, seeds were submerged in various concentrations of growth regulators, such as $GA_3$ and kinetin, as well as minerals such as $KNO_3$ and KCl. An analysis of germination characteristics at $30^{\circ}C$ under light conditions revealed that treatment with 200 and $500mg{\cdot}L^{-1}GA_3$ led to an excellent germination rate of 97.0%. Treatment with $100mg{\cdot}L^{-1}GA_3$ led to a better germination rate than the control as well. However, treatment with kinetin, $KNO_3$, and KCl had no effect on germination, regardless of concentration.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.170-170
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• 2017

The highly valued ornamental plant, Platycodon grandiflorum for. duplex was generated by petaloidy of a calyx of Platycodon grandiflorum. The present investigation was executed to explore the several factors having effects on the germination of pollens with a view to acquire the underlying data for the artificial crossing to cultivate the species of Platycodon grandiflorum for. duplex. Both low and high temperature impaired the germination of Platycodon grandiflorum for. duplex pollens. The good germination rate was observed at the temperature of $25^{\circ}C$. The types and concentrations of carbon sources induced the differences in germination rate. The germination rate increased with the increasing concentration of sucrose and glucose except for fructose. Sucrose and glucose showed the highest results at the concentration of 20%. While fructose demonstrated the similar tendency to sucrose and glucose, it reduced the germination rate at the concentration of 20%. The highest germination rate was observed at the concentration of 15%. The appropriate carbon course for germination of pollens of Platycodon grandiflorum for. duplex was glucose of which germination rate was twice as high as that of sucrose and fructose. The germination rate was reduced substantially when the pH was close to alkali, and the potential germination rate was obtained at pH 6. Boric acid enhanced the germination rate at a lower concentration than the higher concentration.

• 한국산림과학회지
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• 제102권1호
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• pp.136-142
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• 2013

산개나리(Forsythia saxatilis Nakai)의 개화와 결실 특성을 구명하기 위하여, 개화 생리의 기초 연구로서 산개나리 화분의 적정 발아 조건을 제시하고자 본 연구를 실시하였다. 산개나리의 화분 시료는 3월말부터 4월 초 개화시기에 맞춰 채취하였다. 산개나리 화분의 적정 발아 조건을 결정하기 위하여, 최적 배양 온도, 배지 설탕 농도 및 배양 시간을 먼저 선정한 후, 가장 적당한 배지 pH를 결정하였다. 화분 발아율과 화분관 신장은 발아 온도, 배지 설탕 농도 및 발아 시간에 따라 뚜렷한 차이가 있었으며, 발아 온도와 발아 시간, 발아 온도와 배지 설탕 농도 및 발아 시간과 배지 설탕 농도 간에 상호 효과가 나타났다. 산개나리의 화분 발아율은 $10^{\circ}C$에서 가장 높았으며, 배지 설탕 농도가 높을수록 발아율은 증가하였으나, 발아 시간과는 상관이 없었다. 또한, 15% 이상의 설탕 농도에서는 화분 발아율과 화분관 신장이 큰 차이를 나타내지 않았고, 화분 살포 후 24시간 이상이 경과한 후에도 화분 발아율이 큰 차이를 보이지 않았다. 화분 발아율은 pH 5에서 20.8%로 가장 높았으며, pH 6에서 3.8%로 가장 낮았다. 따라서 산개나리 화분의 적정 발아 조건은 발아 온도 $10^{\circ}C$, 배지 설탕 농도 15% 및 배지 pH 5인 것으로 나타났다. 또한, 화분 발아 조사는 화분 살포 24시간 후에 실시하는 것이 가장 바람직한 것으로 판단된다.

• 아시안잔디학회지
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• 제23권1호
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• pp.23-34
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• 2009

톨 훼스큐 종자의 발아율, 발아패턴 및 발아특성은 생육환경과 품종에 따라 차이가 크게 나타났다. 자연 실온 환경에서 발아력은 ISTA 변온과 약간 다르게 나타났고 최종 발아율은 $85.25{\sim}97.00%$ 사이로 다양하였다. 톨 훼스큐의 발아패턴은 최초 발아의 경우 ISTA 변온에 비해 $2{\sim}3$일 정도 늦은 치상 후 $7{\sim}9$일 사이에 시작되었다. 최초 발아 시기는 공시 품종 간 1일 정도 차이가 있었으며, Arid III 및 Olympic Gold 품종이 $7{\sim}8$일 사이로 다소 빨랐다. 발아율 50%에 도달한 기간은 최초 발아 후 $3{\sim}4$일 경과 후인 치상 후 $11{\sim}12$일 사이로 나타났으며, 이것은 ISTA 변온에 비해 $3{\sim}4$일 정도 늦은 것이었다. 또한 실무적으로 요구되는 발아율 76% 도달기간은 ISTA 변온에 비해 품종에 따라 $1.55{\sim}4.77$일 정도 늦은 치상 후 $12.38{\sim}14.29$일 사이로 나타났다. 공시 품종 중 75% 발아율 도달 기간이 가장 빠른 품종은 Olympic Gold 품종이었고, 반대로 가장 느린 종류는 Tar Heel II 품종이었다. 톨 훼스큐 품종 간 발아특성 및 발아패턴의 결과를 종합적으로 고려할 때 Olympic Gold 품종이 가장 우수한 것으로 사료되었다. 본 실험을 통해 밝혀진 품종 간 생리 생태적 특성과 ISTA 변은 및 자연 실온 조건 간 비교 자료는 골프장 등 잔디밭 조성 시 실무적인 기초 자료로 유용하게 활용될 수 있을 것이다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.172-172
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• 2017

Pollen germination and its' viability in bellflower hybrid system are of great importance. The present study was conducted to investigate the several factors underlying the pollen germination of Platycodon grandiflorum and obtain the basic data for effective artificial pollination for the production of sound specifies. The pollens of Platycodon grandiflorum started germination from one hour after planting, and the germination was actively progressed as time dependent manner. For lighting conditions, the germination of pollens under the light was faster by more than twice than that without the light. Furthermore, the germination was better in the high temperature rather than in the low temperature. The germination rate was higher in the $30^{\circ}C$. For the carbon source, the germination rate was better at the concentration of 15% regardless of the kinds. In particular, the highest value was observed with glucose. The germination rate was decreased substantially as the increasing with the higher pH. The dynamic germination of pollens was observed at the pH 5. With respect to the growth regulator, the higher concentration of NAA induced the higher the germination rate. $GA_3$ showed a good germination rate in $0.05mg{\cdot}L^{-1}$. Meanwhile, for kinetin, lower concentration increased the germination rate, unlike NAA. The higher concentrations of boric acid degraded the germination rate, and the addition of boric acid of $10mg{\cdot}L^{-1}$ demonstrated higher germination rate than the addition of other growth regulators. Notably, the addition of asparaginic acid exhibited the similar results in all test sectors regardless of concentration, whereas a little higher result was observed in the high concentration sector. Taken together, the results concluded that the boric acid was considered as one of the essential minerals that played an important role on the germination of pollens.