• Archives of Pharmacal Research
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• 제8권4호
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• pp.197-203
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• 1985

It was attempted to observe the effect of garlic on the hepatic glutathione s-transferase and glutathione peroxidase activity in this study. Glutathione s-transferase (EC 2.5.1.18) are thought to play a physiological role in initiating the detoxication of potential alkylating agents, inclnding pharmacologically active compounds. Glutathione peroxidase (EC 1. 11. 1. 9) might play an important role in the protection of cellular structures against oxidative challenge. The activities of glutathione s-transferase and glutathione peroxidase in rat liver were increased by the treatment of garlic juice. Allicin fraction, heat-treated allicin fraction and garlic butanol fraction markedly inhibited glutathione s-transferase activity in vitro, whereas glutathione peroxidase activity was significantly increased in heat-treated allicin fraction and garlic butanol fraction.

• 대한약리학회지
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• 제22권2호
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• pp.144-150
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• 1986

마늘성분중의 하나인 diallyl disulfide가 간 glutathione peroxidase 활성에 어떠한 영향을 주는가를 관찰하여 다음과 같은 성적을 얻었다. 여러 농도의 diallyl disulfide를 흰쥐에 투여 하였을때 혈청 transaminase의 활성은 변동이 없었으며 또한 간 glutathione의 함량도 변화되지 않았다. 그러나 간 가용성분획의 glutathion peroxidase 활성은 현저히 증가되었다. 시험관 내에서 간 가용성분획의 glutathione peroxidase의 활성은 $15{\mu}g/m{\ell}$ 이상의 diallyl disulfide를 첨가 하였을때 현저히 증가되었으며, 동력학적인 측면에서 관찰하였을때 diallyl disulfide$(20{\mu}g/m{\ell})$에 의해 Km치는 별 변화가 없었으나 Vmax치는 현저히 증가되었다. 이상의 실험결과에서 마늘 성분 중에 glutathione peroxidase 활성을 증가시키는 성분은 allicin의 분해산물인 diallyl disulfide에 기인된 것으로 사료되며 또한 이 성분은 효소의 기질결합 부위가 아닌 다른 부위에서 효소의 활성을 조절해 줄 것으로 생각되어진다.

• BMB Reports
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• 제43권3호
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• pp.170-175
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• 2010

Chaperone;Glutathione peroxidase;Peroxiredoxin;Schizosaccharomyces pombe;Thioredoxin peroxidase;To investigate the differences in the functional roles of peroxiredoxins (Prxs) and glutathione peroxidase (GPx) of Schizosaccharomyces pombe, we examined the peroxidase and molecular chaperone properties of the recombinant proteins. TPx (thioredoxin peroxidase) exhibited a capacity for peroxide reduction with the thioredoxin system. GPx also showed thioreoxin-dependent peroxidase activity rather than GPx activity. The peroxidase activity of BCP (bacterioferritin comigratory protein) was similar to that of TPx. However, peroxidase activity was not observed for PMP20 (peroxisomal membrane protein 20). TPx, PMP20, and GPx inhibited thermal aggregation of citrate synthase at 43$^{\circ}C$, but BCP failed to inhibit the aggregation. The chaperone activities of PMP20 and GPx were weaker than that of TPx. The peroxidase and chaperone properties of TPx, BCP, and GPx of the fission yeast are similar to those of Saccharomyces cerevisiae. The fission yeast PMP20 without thioredoxin-dependent peroxidase activity may act as a molecular chaperone.

• Parasites, Hosts and Diseases
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• 제30권4호
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• pp.355-358
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• 1992

Oxygen radical은 생물체의 산소대사 과정의 부산물로 생성되어 세포내 여러 성분을 불환성화시키거나 미생물에 대한 방어기전으로 작용한다. 기생충에 존재하는 antioxidant enzyme은 숙주의 방어기전에서 유리하는 oxygen radical의 독성을 제거하므로 이 효소의 활성도는 기생충의 생존에 영향을 미친다고 생각하고 있다. 폐흡충은 피낭유충이 종숙주에 침입한 다음 숙주내 조직이행 시기를 거쳐서 폐에 도달하여 성충이 되므로 이 과정에서 각 시기별 산소독성과 이에 대항하는 효소의 환성이 다를 것으로 추정하였다. 폐흡충의 피낭유충과 감염후 4주, 8주, 12주에 얻은 충체의 추출물을 조효소(조효소)로 하여 SOD, catalase, peroxidase, glutathione peroxidase의 활성도를 측정하였다. 각 효소의 비환성도(specific activity) 중 catalase는 피낭유충에서 최고치였으며, SOD 와 peroxidase는 4주 충체에서 가장 높았고, glutathione peroxidase는 8주 충체에서 높았다. 이들 4가지 antioxidant효소의 비환성도는 감염 12주인 성충에서 모두 낮게 측정되어 조직 이행시기의 충체에서 더 높은 효 소 활성도를 지니고 있음을 알 수 있었다.

• 대한수의학회지
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• 제30권3호
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• pp.271-275
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• 1990

The tests related to red cell fragility were performed. Samples of blood anticoagulated with heparin were obtained from Korean native cattle in Chonbuk region abattoir, and classified by the district(Kun) with reference to breeding location. Hemolysis test for red cell fragility was performed with whole blood and glutathione peroxidase activity was measured spectrophotometrically. Blood concentration of selenium, inorganic component of glutathione peroxidase, was also determined fluorophotometrically. The results obtained were summerized as follows; 1. Percent hemolysis of erythrocytes ranged from 13.53 to 20.74%, and its mean Palue was low as $17.11{\pm}9.91%$. Means in all were not district(Kun) in Chonbuk region significantly different. 2. Glutathione peroxidase activity ranged from 2,881 to 4,000mU/ml, and high mean values, $3,352{\pm}1,872mU/ml$, reflected low percent hemolysis. 3. There was a highly negative correlation between the red cell fragility(Y) and blood glutathione peroxidase activity(X). The linear regression equation for these data was: Y=29.86-3.75X with a correlation coefficient of r=-.6886 (p<0.01) 4. Blood selenium concentration ranged from 0.16 to $0.24{\mu}g/ml$, and mean values was normal level as $0.2{\pm}0.11{\mu}g/ml$. 5. There was a highly positive correlation between blood selenium concentration(X), and blood glutathione peroxidase activity(Y). The linear regression for these data was: Y=230+15,790X, with a correlation coefficient officient of r=0. 8635.

• Preventive Nutrition and Food Science
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• 제8권1호
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• pp.36-39
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• 2003

Many defined cell culture media were formulated over 3() years ago and may be deficient in certain micronutrients whose essentiality has only subsequently been recognised. The objective of this study was to evaluate whether alpha-minimal essential medium (MEM) supplemented with 10% foetal bovine serum contained sufficient selenium for optimal activity of the selenium containing enzymes cytosolic glutathione peroxidase (cGPx) and phospholipid hydroperoxide glutathione peroxidase (PHGPx) in cultured Chinese hamster ovary (CHO) cells. Additionally, the effect of zinc deficiency and metallothionein (MT) overexpression on cGPx and PHGPx activity was studied. The addition of 100 nM of selenous acid to the culture medium increased cGPx expression by 10-fold and PHGPx by about 2-fold in both wild-type CHO-K1 cells and CHO-K1 cells overexpressing mouse MT-1. Zinc deficiency had no significant effect on enzyme activity, but cells overexpressing mouse MT-1 had higher levels of cGPx activity. Zinc deficiency decreased cell survival but overexpression of MT-1 was partially protective, probably because its presence in quantity favoured the uptake, sequestration and cellular retention of any remaining zinc. This study demonstrates that selenium in complete alpha-MEM is insufficient for optimal cGPx and PHGPx activity and may compromise the cellular response to oxidative stress.

• 대한한방내과학회지
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• 제21권1호
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• pp.108-115
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• 2000

Objective : To investigate the hepatoprotective effects of Gami-oryungsan on the liver damage induced by bromobenzene. Method : The development of fibrosis and acute liver injury was examined by the chemical analysis of AST, AL T, ${\gamma}$-GTP . and epoxide hydrolase glutathione S-transferase glutathione peroxidase enzyme activity, lipidoperoxide levels, glutathione levels were measured and oberved. Results : The increasing levels of lipidoperoxide was decreased proportionally according to dose of extract GO. Epoxide hydrolase glutathioneS-transferase glutathione peroxidase enzyme activity highly increased in GO pre-acupunctured group compared with the group treated with only bromobenzene. The increase of serum AST, AL T, ${\gamma}$-GTP enzyme activity of mice by bromobenzene was inhibited by the administration of GO. Lipidoperoxide levels in rat's liver decreased compared to the case of bromobenzene-treated group. The levels of Glutathione decreased by bromo benzene were increased highly in GO pre-acupunctured group. Conclusion : These results suggest that GO extract recovers the damage of liver due to bromobenzene intoxication by decreasing the lipid peroxidation AST AL T ${\gamma}$-GTP enzyme activity and increasing epoxide hydrolase glutathioneS-transferase glutathione peroxidase enzyme activity, glutathione levels.

• Journal of Nutrition and Health
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• 제30권9호
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• pp.1061-1066
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• 1997

This study was undertaken to evaluate the effect of 4 weeks of $\alpha$-tocopherol(800 I.U./d) supplementation on oxidant stress of eleven female aerobic -majoring students during rest and exercise. Changes in the activity of the antioxidant enzyme glutathione peroxidase were also studied. Serum $\alpha$-tocopherol concentration was significantly increased with vitamin E supplementation(710.1$\pm$113.8$\mu\textrm{g}$/dl vs. 1,485,8$\pm$105.2$\mu\textrm{g}$/dl). In addition, serum MDA concentration, an index of lipid peroxidation, significantly decreased after vitamin E supplementation. However, MDA values after exercise increased to pre-supplementation levels. Serum glutathione peroxidase activity significantly increased with vitamin E supplementation. The enzyme activity showed a trend toward decrease after exercise. Serum cholesterol values were not significantly affected by vitamin E supplementation. However, serum triglycerides significantly increased after supplementation against oxidative stress during resting periods. These supplements appraently work by decreasing lipid peroxidation and increasing glutathione peroxidase activity. However, vitamin E supplementation did not prevent exercise-induced increases in lipid peroxidation.

• 대한수의학회지
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• 제31권1호
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• pp.41-47
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• 1991

The protective of influences of sodium selenitc ($Na_2SeO_3$) against the methemoglobinemia with sodium nitrite were investigated on hemoglobin, methemoglobin, glutathione peroxidase and NADH-methemoglobin reductase activity in rabbits which were given 0,1,3 and 9ppm sodium selenite of drinking water for a week. Dietary selenium did not alter total hemoglobin in the blood of rabbits. Selenium was found to decrease nitrite-induced methemoglobin in a dose-dependent manner. The glutathione peroxidase activity was also increased by selenium in all the experimental groups. However, the NADH-methemoglobin reductase activity by selenite did not show significant differences as concerns the methemoglobinemia. These results showed that selenium could inhibit nitrite-induced methemoglobinemia. Its influence of inhibition is suggested that the effect of the reduction of methemoglobin was greatly stimulated by glutathione peroxidase activity.

• Applied Microscopy
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• 제19권2호
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• pp.99-118
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• 1989

The cardiotoxic effects of acute and chronic administration of adriamycin (ADR) were evaluated in A/J Swiss albino mice. In acute studies, male mice received intravenous ADR, 5mg or 15mg/kg per day for 3 or 1day and were sacrifice 12 hours later. Because the glutathione-glutathione peroxidase system is major pathway for free radical detoxication, glutathione levels and glutathione peroxidase activity was measured. In acute studies, ADR-treated mice exhibited significantly decreased levels(p<0.05) of total glutathione and unchanged levels of oxidized glutathione and percentage of oxidized glutathione. The earliest myocardial fine structural alterations included swelling and degeneration of mitochondria and dilatation of sarcoplasmic reticulum at all dosage of acute models. In chronic studies, mice received 5mg/kg ADR once a week for up to 16 weeks. Levels of total and reduced glutathione were decreased significantly(p<0.01) and oxidized glutathione and percentage of oxidized glutathione were increased significantly (p<0.05). Chronic myocardial lesions included perinuclear vacuolization, seperation of myofibrils and the fasciae adherens of intercalated disc and hypercontraction band within myocyte. Glutathione peroxidase activity reduced significantly (p<0.01) in any group of acute and chronic ADR-treated animals. Test for lipid peroxidation(malondialdehyde) was increased significantly(P<0.01). Thus, we conclude 1) ADR significantly lowers glutathione levels in heart tissue, and 2) cellular damage progress produced by alteration of this system in mouse models of ADR cardiotoxicity. These results suggest that the glutathione-glutathione peroxidase system may be involved in the modulation of ADR-induced cardiotoxicity.