• Journal of the East Asian Society of Dietary Life
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• v.15 no.2
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• pp.207-212
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• 2005

To determine changes of chlorophyll and potato glycoalkaloid(PGA) contents in potato tubers after lightening, Dejima potatoes cultivated in Korea were used and PGA was analyzed by HPLC. Potatoes were stored under fluorescent and UV light exposure or darkness for 7 days at $5^{\circ}C$. The contents of chlorophyll and glycoalkaloid in the peel of potatoes exposed to fluorescent light were increased to $84\%,\;69.5\%$ respectively compared to potatoes stored without lightening. While a marked effect of lightening on the peel of potatoes exposed to fluorescent light, UV light lead to no change of chlorophyll and less increase in glycoalkaloid than fluorescent did. The increase of chlorophyll was provoked by only fluorescent light. Cortex part did not accumulate any chlorophyll or glycoalkaloid.

• Proceedings of the Korean Society of Food and Cookery Science Conference
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• 2003.05a
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• pp.102-102
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• 2003

감자에는 적은 양이지만 자연독의 종류인 glycoalltaloid가 함유되어 있다. glycoalkaloid의 대표적인 성분은 a-solanine과 B-chaconine으로 이들을 20rng 이상 섭취할 때에는 건강에 유해하고 그 함량이 많을 시에는 쓴맛의 원인이 된다. 지금까지의 생감자에 함유된 glycoalkaloid에 관한 연구는 몇편 보고된 바 있으나, 실제 소비자가 구매하여 섭취하는 감자 가공제품에 관한 연구는 찾아보기드문 실정이다. 따라서 본 연구는 시판 감자 가공품을 시료로 하여 그에 함유되어 있는 glycoalkaloid의 함량을 조사히여 시판 감자 가공품에 대한 몇 가지의 정보를 제공하고자 한다. (중략)

• Journal of the East Asian Society of Dietary Life
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• v.16 no.5
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• pp.600-606
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• 2006

This survey was conducted to investigate the usage of potato and recognition of glycoalkaloid among residents aged from their teens to over fifties in Daegu city and Gyeongbuk Province. The preferred purchase places for the respondents were traditional markets (41.8%), big discount markets (23.8%), and supermarkets (14.9%), in order. Freshness (52.1%) was the most important criterion followed by size (12.5%), sprout (10.1%) and producing district (6.6%), in order. Most (77.6%) respondents preferred small amount below $2{\sim}3kg$ per purchase. Potato recognition revealed that respondents knew relatively well that potato sprouts contain toxins (M=4.30), that the major potato toxin is solanine (M=3.86) and that potato contains toxins when its color turns to green (M=3.70). However, respondents did not recognized well that the potato peel contains toxins (M=3.00), or that this toxin is chaconine (M=2.48).

• Korean Journal of Food Science and Technology
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• v.16 no.4
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• pp.388-391
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• 1984

The purpose of this report was to investigate change in glycoalkaloid content (${\alpha}-chaconine$, ${\alpha}-solanine$) which contained in the cortex and medulla part during storage of two varieties of potato, Irish Cobbler and May Queen at $1^{\circ}C$ and $20^{\circ}C$. The tubers of both varities stored at $20^{\circ}C$ showed sprouting and ${\alpha}-chaconine$ and ${\alpha}-solanine$ contained in cortex part increased, particularly the extent of increase was more noticeable in May Queen than in Irish Cobbler. The content of glycoalkaloid in tubers stored at $1^{\circ}C$ also increased continuously. A small amount of ${\alpha}-chaconine$ was detected in the medulla part of both varieties, but ${\alpha}-solanine$ was not detected at all.

• Korean Journal of Food Science and Technology
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• v.16 no.4
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• pp.383-387
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• 1984

The difference of glycoalkaloids content on various parts of May Queen and Irish Cobbler potatoes was determined. ${\alpha}-Chaconine$ and ${\alpha}-Solanine$ were isolated from the glycoalkaloids by use of high performance liquid chromatography. It was found that the 99% of the total glycoalkaloids was existent in cortex part of all varieties. Glycoalkaloids content was higher in apical or basal part than the middle part. ${\alpha}-Chaconine$ content of the cortex showed no differences among parts of the potato in both varieties. ${\alpha}-Solanine$ was not detected in medulla part. The potatoes were classified into 4 groups depending on the weight and the glycoalkaloids content of the middle part. As the weight of the potato decreased the glycoalkaloids content of cortex part increased. Glycoalkaloids content was lower in medulla part and no constant tendency was observed.

• Culinary science and hospitality research
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• v.12 no.4 s.31
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• pp.247-258
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• 2006

This study was carried out to identify the change of glycoalkaloid contents and ascorbic acid in eggplant by parts and cooking methods. Content of glycoalkaloid by part of eggplant was the most in step part(A) as $31.1{\mu}g$ but it was shown that (A) and (C) contained more solamargine($6.9{\mu}g$) than solasonine, while (B) contained more solasonine($18.4{\mu}g$) than solamargine($6.9{\mu}g$). By cooking method, content of solamargine in eggplant varied; steaming ($6.2{\mu}g$), boiling ($7.0{\mu}g$), sauteing ($2.5{\mu}g$), microwave ($2.0{\mu}g$). Control group had $11.4{\mu}g$ solamargine and it contained solamargine most among them when boiled. Content of solasonine was in order of control ($4.88{\mu}g$)>steaming($3.40{\mu}g$)>microwave($1.2{\mu}g$)>boiling($0.6{\mu}g$)>sauteing(n.d.), and steaming showed the highest figure. As for content of ascorbic acid by part of eggplant, stem part had the most. That is, content of ascorbic acid decreased as from stem part under. Content of ascorbic acid of eggplant decreased greatly by cooking manipulation like other vegetables did, and the remaining amount showed control> sauteing> microwave> boiling> steaming in order.

• Journal of Plant Biotechnology
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• v.37 no.1
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• pp.96-101
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• 2010

Leaf explants of the Solanum hainanense plant, grown in vitro, were cultured in basal Murashige and Skoog (MS) media supplemented with 0.5 mg/L kinetin and 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) for callus initiation. For maintenance and proliferation, the callus was cultured on MS medium supplemented with 1 mg/L 6-benzylaminopurine (BAP) and 0.5 mg/L 2,4-D. The glycoalkaloid content in the callus was at its maximum after ten weeks of culture (188.65 mg/g), whereas that of the one-year-old control was 22.22 mg/g in the root and 5.99 mg/g in the stem. The glycoalkaloid extracted from the callus inhibited the activity of collagenase on collagen gel. High performance liquid chromatography (HPLC) analysis showed that biotransformation occurred when a callus was grown on medium supplemented with various carbon sources. These results suggest that callus of S. hainanense is a good material for production of glycoalkaloid.

• Journal of the Korean Home Economics Association
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• v.42 no.4
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• pp.187-194
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• 2004

The concentration of potato(Solanum tuberosum L.) glycoalkaloids(PCA) (i.e., ${\alpha}$-chaconine and ${\alpha}$-solanine) in Korean cultivated potato plant, and in the different varieties, parts and weights of the potato tubers, were determined by high-performance liquid chromatography(HPLC). The highest concentrations of PGA in potato plants were found in the roots, followed by the stems and leaves. A large quantity of PGA existed in the periderm of 'Atlantic' potatoes, whereas 'Irish Cobbler' contained the lowest amount in five cultivated potato tubers.'Irish Cobbler' potatoes were divided into three parts and the PGA content in each part was determined. It was found that both of the end parts of the potatoes contained higher PGA than the middle part. The PGA contents in four different sizes of potatoes increased toward the smaller size. Thesignificantly high level of PGA was contained in the smallest size potato tubers, which have been used widely for Korean cooking.

• Korean journal of food and cookery science
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• v.23 no.2 s.98
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• pp.245-251
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• 2007

The purpose of this study was to examine how potato glycoalkaloid(PGA) contents changed as storing temperatures and periods changed for potatoes(Superior) cultivated in Korea. Some potatoes were stored at $5^{\circ}$C for 80 days, and others were stored at $20^{\circ}$C for 40 days. PGA contents were measured every 20 days for the potatoes stored at $5^{\circ}$C and 10 days for those stored at $20^{\circ}$C. The results can be summarized as follows. The amounts of a-chaconine and a-solanine measured on the first day of storage were 54.22 mg/100 g and 26.57 mg/100 g, respectively. Thus, the a-chaconine content was almost twice as much as the a-solanine content. The sprouts of potatoes stored at $5^{\circ}$C grew by 0.4 cm in 20 days, 1 cm in 40 days, 1.8 cm in 60 days, and 5.2 cm in 80 days. Furthermore, the PGA content increased by 3.5% in 20 days, 11.6% in 40 days, 23.4% in 60 days, and 41.4% in 80 days, compared to the PGA content on the first day. The sprouts of potatoes stored at 20 grew by 0.5 cm in 10 days, 2.3 cm in 20 days, and 7.4 cm in 40 days. Furthermore, PGA content increased by 12.5% in 10 days, 36.6% in 20 days, 44.8% in 30 days and 48.4% in 40 days, compared to the PGA content on the first day. Thus, we determined that potatoes stored at $20^{\circ}$C showed faster sprout growths and faster increases in PGA content than those stored at $5^{\circ}$C.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1995.06b
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• pp.55-75
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• 1995

Induction of HMG-Co A reductase (HMGR) is essential for the biosynthesis of sesquiterpenoid phytoalexins and steroid derivatives in Solanaceous plants following wounding and pathogen infection. To better understand this complex step in stress-responsive isoprenoid synthesis, three classes of cDNAs for HMGR (hmg1, hmg2, and hmg3) were isolated from a potato tuber library. The potato cDNAs had extensive homology in open reading frames but had low homology in the 3'-untranslated regions. RNA gel blot analysis using gene-specific probes revealed that hmg1 is induced by wounding but wound induction is strongly suppressed by arachidonic acid or by inoculation with Phytophthora infestants. In contrast, hmg2 and hmg3 are slightly induced by wounding and strongly enhanced by arachidonic acid or inoculation. The induction and suppression of HMGR genes parallel the suppression of steroid and stimulation of sesquiterpenoid accumulations observed in earlier investigations. Treatment of the tuber disks with a low concentration of methyl-jasmonate doubled the wound induced accumulation of hmg1 transcripts and steroid-glycoalkaloid accumulation, but did not affect the abundance of transcripts for hmg2 or hmg3 nor induce phytoalexins. High concentration of methyl-jasmonate suppressed hmg1 mRNA and steroid-glycoalkaloid accumulation, induced hmg3 mRNA, and did not elicit phytoalexins. Lipoxygenase inhibitors suppressed the accumulation of of hmg1 transcripts and steroid-glycoalkaloids, which were restored by exogeneous methyl-jasmonate. Methyl-jasmonate applied together with arachidonic acid enhanced the elicitor induced accumulation of sesquiterpenes and sustained steroid-glycoalkaloid levels with transcript levels for the various HMGR mRNAs equal to or greater than wound-only treatment. These results domonstrate that the consequences of wound- and pathogen-responses of plants are different at the levels of gene expression and associated secondary metabolism.