• Asian-Australasian Journal of Animal Sciences
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• 제31권5호
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• pp.738-747
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• 2018

Objective: In a previous study, analysis of Illumina sequenced metagenomic DNA data of bacteria in Vietnamese goats' rumen showed a high diversity of putative lignocellulolytic genes. In this study, taxonomy speculation of microbial community and lignocellulolytic bacteria population in the rumen was conducted to elucidate a role of bacterial structure for effective degradation of plant materials. Methods: The metagenomic data had been subjected into Basic Local Alignment Search Tool (BLASTX) algorithm and the National Center for Biotechnology Information non-redundant sequence database. Here the BLASTX hits were further processed by the Metagenome Analyzer program to statistically analyze the abundance of taxa. Results: Microbial community in the rumen is defined by dominance of Bacteroidetes compared to Firmicutes. The ratio of Firmicutes versus Bacteroidetes was 0.36:1. An abundance of Synergistetes was uniquely identified in the goat microbiome may be formed by host genotype. With regard to bacterial lignocellulose degraders, the ratio of lignocellulolytic genes affiliated with Firmicutes compared to the genes linked to Bacteroidetes was 0.11:1, in which the genes encoding putative hemicellulases, carbohydrate esterases, polysaccharide lyases originated from Bacteroidetes were 14 to 20 times higher than from Firmicutes. Firmicutes seem to possess more cellulose hydrolysis capacity showing a Firmicutes/Bacteroidetes ratio of 0.35:1. Analysis of lignocellulolytic potential degraders shows that four species belonged to Bacteroidetes phylum, while two species belonged to Firmicutes phylum harbouring at least 12 different catalytic domains for all lignocellulose pretreatment, cellulose, as well as hemicellulose saccharification. Conclusion: Based on these findings, we speculate that increasing the members of Bacteroidetes to keep a low ratio of Firmicutes versus Bacteroidetes in goat rumen has resulted most likely in an increased lignocellulose digestion.

• 한국초지조사료학회지
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• 제38권2호
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• pp.135-139
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• 2018

본 연구는 제주조릿대의 사초생산성과 사료가치평가를 진행하여 조릿대의 활용도를 높임과 동시에 현재 한라산에서 발생하고 있는 조릿대로 인한 식물종 다양성 감소문제를 완화하는데 도움이 되고자 실시하였다. 조릿대의 사초생산성은 실험결과 5월과 6월기간에 비교적 우수한 재생력을 보였으나, 예취가 반복 될수록 재생능력이 감소하여 생산성이 떨어지는 경향을 나타내었다. 조릿대의 일반성분 분석결과 조단백질은 가을철 함량보다 봄철에서 비교적 높은 함량을 보였으며 6월에서 $8.64{\pm}0.20%$로 다른 기간에 비해 비교적 높은 함량을 나타내었으며 가장 낮은 NDF, ADF의 함량은 6월에서 각각 $77.90{\pm}0.25%$, $42.33{\pm}0.49%$로 나타나 섬유소 함량이 매우 높은 것으로 판단된다. 조릿대는 NDF와 ADF의 함량은 높으나, 볏짚에 비해 조단백질 함량은 비교적 높은 것으로 판단되며 가축의 특성에 맞추어 급여한다면 이용성이 있을 것으로 여겨진다. 조릿대를 사료원으로 이용하기 위해서는 섬유소 분해능력이 좋은 흑염소 같은 가축 또는, 조릿대에 대한 기호성이 우수하고, 소화율이 비교적 우수한 말에게 급여하는 것이 효율적일 것으로 여겨진다. 조릿대의 사료화를 위해서는 보다 다양한 축종을 대상으로 추가 연구가 진행되어야 할것으로 판단된다.

• 한국초지조사료학회지
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• 제26권4호
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• pp.221-226
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• 2006

본 시험은 2003년 9월부터 2005년 12월까지 충남대학교 생명과학대학내 부속 초지시험포장에서 수행하였다. 공시된 혼파초지는 상번초형(tall type) 혼파초지(orchardgrass, Potomac 50+tall fescue, Fawn 20+ Kentucky bluegrass, Kenblue 10+red clover, Kenland 20%)와 잔디형(turf type) 혼파초지(Kentucky bluegrass, Newport 60+Tall fescue, Reboil II 20+ perennial ryegrass, Palmer II 20%}의 두 처리를 두어 시험하였다. 얻어진 시험결과는 다음과 같다. 2년 평균 건물수량은 상번초형 혼파초지가 12,721 kg/ha으로 잔디형 혼파초지의 11,275 kg/ha 보다 높은 결과를 보였다(p<0.05). 잔디형 혼파초지는 상번초형 혼파초지에 비하여 CP 함량과 건물소화율(DMD)이 높은 반면에 NDF와 ADF, cellulose 및 lignin 함량은 낮은 결과를 보였다(p<0.05). 2년 평균 조단백질수량(CPDM)과 가 소화건물수량(DDM)은 상번초형 혼파초지가 잔디형 혼파초지에 비하여 높은 결과를 보였다 (p<0.05). 이상의 결과로 보아, 잔디형 혼파초지는 상번초형 혼파초지에 비하여 목초의 사료가치는 높았으나, 건물수량이 낮아서 조단백질수량과 가소화건물수량이 낮은 결과를 가져왔다. 그러나 잔디형목초는 상번초형 혼파초지에 비하여 사료가치가 높고, 연간 13회 예취 시 수량과 밀도를 고르게 유지할 수 있었던 것으로 보아 추후 말이나 유산양과 같은 가축의 방목이용 가능성을 타진할 필요가 있다고 판단된다.

• Journal of Ecology and Environment
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• 제43권2호
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• pp.85-97
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• 2019

Background: Despite the increasing role of dry forests in climate change adaptation and mitigation, these versatile resources has got less attention in the national and regional planning, their potential to enhance the local and national economy has been overlooked, and their contribution to sustainable environmental management has not been recognized. Hence, the objective of this study was to assess the socioeconomic contribution of dry forests and forest products to climate change adaptation in the Liben Woreda, Southern Oromia region of Ethiopia. Methods: For this study, an integrated qualitative and quantitative approach was used. A total of 74 households from villages in the Bulbul, Boba, and Melka-Guba kebeles were randomly selected for the household survey. Results: Results showed that 75% of the respondents in the area indicated that climate change has become their major sources of vulnerability, where drought has been manifested in the form of crops failure and massive death of livestock particularly cattle species. The main income strategies of the study households include livestock, crop, forests such as gum and resins, firewood and charcoal and non-farm activities such as in the form of petty trade, wage and aid. The average total household income was ETB 11,209.7. Out of this, dry forest income constituted 15% of the total income. In addition to using dry forests as rangeland for livestock, the communities collect wood for construction, fodder, traditional medicine, and forest food both for subsistence and for sale. On the other hand, dry forest products could be considered as less vulnerable, rather resilient livelihood strategies to climate- and environment-related risks compared to livestock and crop production such as in the face of drought periods. More than 48.6% of the households argued that the income generated from dry forests increased substantially due to increment in the level of engagement of family members in forest based income activities. On the other hand, 35.8% of the households responded that livestock production, particularly camels and goats, have been making the livelihood strategies of the respondents more resilient indicating the shift made from grazers browsers to livestock. In general trends show that, the trends of livelihood dependency on dry forest were highly increasing indicating the importance of dry forest income in responsse to frequent droughts. Conclusions: Dry forest income has been becoming crucial livelihood staretgy in response to frequent droughts in the study area and hence, it is important to improve the management of dry forests for livelihood enhancement, while also securing their long-term ecological functions.

• 대한수의학회지
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• 제43권3호
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• pp.493-500
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• 2003

This study was performed to determine the optimal dose of ozone for wound healing. Twenty Korean black goats were allocated to 4 groups. The skin wound with $1{\times}1cm$ was induced over the hoof. For 5 minutes per day, 0.1 ppm (Group I), 1 ppm (Group II) and 6 ppm (Group III) ozone gas was applied. In control group, 5 ml of normal saline was applied daily on the wound. Wound square was determined using color image analyzer at day 0, 4 and 14. Soft tissue depth of wound was determined using radiograph. Before the application of ozone gas, $1{\times}1cm$ of skin tissue was resected and observed for histopathologic findings. To determine the healing effect, $2{\times}1cm$ of wound tissue was resected and observed for histopathologic findings at day 14. In mean squares of wound, all experimental groups revealed more reduced mean squares than control group at day 4, and especially Group II (p<0.05) and Group III (p<0.05) revealed significant reduction. At day 14, Group I and Group II (p<0.05) revealed more reduced mean squares than control group, but Group III revealed less reduced mean squares than control group. In soft tissue depth of wound, all experimental groups and control group revealed swelling at day 4, compared with day 0. At day 14, all experimental groups and control group revealed reduction, compared with day 4, and especially Group II (p<0.05) revealed significant reduction. In histopathological findings, inflammation findings in epidermis and dermis, hemorrhagic finding in epidermis were revealed in control group. In Group I, inflammation finding was reduced, compared with control group. Formation of scab and many reepithelialization of epidermis were revealed. In Group II, inflammation findings in epidermis and dermis which were observed in control group were not found, normal skin tissue was revealed. In Group III, inflammation findings in epidermis and dermis were revealed, reepithelialization of epidermis was not founded. Slow healing process was observed, compared with Group I and II. It was concluded that Group II has excellent promotion effect of wound healing.

• 한국수정란이식학회지
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• 제21권4호
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• pp.339-344
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• 2006

본 연구는 염소 액상 정액의 활용에 적합한 희석액을 검토하여, 국내 염소 인공수정 실용화를 위한 기초 자료를 확보하기 위하여 실시되었다. 두 마리의 흑염소 수컷으로부터 전기 자극법으로 정액을 채취하였다. 채취된 정액은 BTS로 희석하여 500g에서 5분간 원심분리하여 정장을 제거하고, BTS, Modena, Triladyl$^{(R)}$로 희석하여 $4^{\circ}C$ 냉장고나 $17^{\circ}C$ 온장고에서 8일간 보관되었다. BTS와 Modena로 희석된 정자의 운동성은 하루 만에 소멸되었으며, BTS로 희석된 정액을 $17^{\circ}C$에 보관하였을 때 2일째에 30$\sim$45%로 떨어졌으며, Modena에서는 3일째까지 인공수정에 가능한 활력을 유지하였다. 한편 Triladyl$^{(R)}$로 희석하여 $4^{\circ}C$나 $17^{\circ}C$에서 보관한 경우에는 좀더 좋은 생존성을 나타내었으며, 4일까지도 인공수정이 가능한 운동성을 보여주었다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권6호
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• pp.760-768
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• 2004

The present study was undertaken to evaluate the accuracy of ultrasonography in early pregnancy diagnosis in goats. Ultrasonographic scanning with real time B-mode ultrasound machine having 5 MHz linear array transducer was performed on gravid uterus (n=24) obtained from slaughterhouse (Group I). Crown rump length (CRL) measured by ultrasound was found significantly different (p<0.05) with actual CRL measured after dissection in early pregnancy. However, age predicted by ultrasound through the measurement of CRL was found highly correlated (r=0.92) with age measured after dissection through CRL and the weight of fetus. Ages predicted by ultrasound through the measurement of trunk diameter (TD) and uterine diameter (UD) and ages measured after dissection were found highly and equally correlated (r=0.98) and did not differ significantly. Data from six does synchronized (Group II) with PGF2$\alpha$ (Estrumate) at 11 days apart were collected through ultrasound from 17 to 42 days post breding. The correlation between CRL and gestational age was high (r=0.97) in day 30 to 42 post breeding. A high coefficient of correlation (r=0.98) was also observed between predicated age by ultrasound and actual age calculated after kidding. The correlation between CRL and gestational age by the formula Y=(a+bX) i.e. Y=24.42+0.39 X where Y=gestational age and X=CRL, was recorded very high (r=0.99). Accuracy of ultrasonography was lowest on day 17 to 19 (66%) and reached 100% on day 34. Data from 30 does (group III) randomly subjected to only one time ultrasounds scanning to assess the accuracy of pregnancy diagnosis were also obtained. Ages predicted by TD and UD measurements were observed to be non-significantly different with actual age obtained after kidding and correlation between ages predicted by TD and UD measurement with actual age after kidding was found equally and highly correlated (r=0.98). The operator's accuracy in the whole experiment including all three groups was found to be 92%. The sensitivity was 93% and specificity was 86%. From the present study, it was observed that CRL was the most reliable parameter to find out gestational age in early pregnancy and the new formula derived was found very accurate to find out gestational age. TD and UD were also found to be equally reliable parameter to find out gestational age in mid and late stage of pregnancy through ultrasonography. It was concluded that ultrasonography by real time B mode with 5 MHz transrectal transducer was found to be reliable, safe and accurate and practicable means in diagnosing early pregnancy diagnosis as early as 25 days post breeding.

• Asian-Australasian Journal of Animal Sciences
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• 제20권7호
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• pp.1067-1075
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• 2007

Twenty eight 3-4 month old castrated Black Bengal kids (Capra hircus) were used to determine the effects of source and level of dietary copper (Cu) concentration on their performance and nutrient utilization. Cu was supplemented (0, 10, 20 and 30 mg/kg diet DM) as copper sulfate ($CuSO_4$, $5H_2O$) or copper proteinate (Cu-P). Kids were fed a basal diet containing maize (19.5%), soybean (17.0%), deoiled rice bran (56.5%), molasses (4.0%), di-calcium phosphate and salt (1.0% each) and mineral and vitamin mixture (0.5% each) supplements at 3.5% of body weight to meet NRC (1981) requirements for protein, energy, macro minerals and micro minerals, excluding Cu. The basal diet contained 5.7 mg Cu/kg, 122.5 mg Fe/kg, 110 mg Zn/kg, 0.26 mg Mo/kg and 0.32% S. $CuSO_4$ or Cu-P was added to the basal diet at the rate of 10, 20 and 30 mg/kg. Kids were housed in a well ventilated shed with facilities for individual feeding in aluminum plated metabolic cages. Blood samples were collected from the jugular vein on d 0, 30, 60 and 90 to determine hemoglobin (Hb), packed cell volume (PCV), total erythrocyte count (TEC), total leukocyte count (TLC) and serum enzymes (alkaline phosphatase, alanine transferase and aspertate transferase). A metabolism trial of 6 days duration was conducted after 90 days of experimental feeding. Statistical analysis revealed that source and level of Cu supplementation improved live weight gain (p<0.04) and average daily gain (p<0.01). No significant contribution of source and level of Cu to alter serum serum enzymes was evident. Goats fed Cu-P tended to have higher Hb, PCV and TEC than with $CuSO_4$ supplementation. Cu-P increased digestibility of ether extract (EE, p<0.02) and crude fiber (p<0.05) and showed an increasing trend (p<0.09) for digested crude protein (CP) and crude fiber (CF). Supplemental dose of Cu linearly improved (p<0.02) digestibilities of dry matter (DM), organic matter (OM), EE and nitrogen free extract (NFE). Though the absorption of nitrogen (N) was not affected (p>0.10) by both source and dose of Cu, N retention was affected (p<0.04) and there was a significant $Source{\times}Dose$ interaction (p<0.05). Final body weight (BW) was not influenced (p>0.10) by the source of Cu but increasing dose of Cu increased (p<0.04) the BW of kids. TDN intake (g/kg $W^{0.75}$) was higher (p<0.05) with the increased dose of Cu and there was a significant $Source{\times}Dose$ interaction. It was concluded that supplementation of Cu from different sources and varying dose level in a concentrate based diet may improve performance, nutrient utilization and plane of nutrition in castrated Black Bengal kids. The effects on performance and nutrient utilization are more pronounced with Cu-P than $CuSO_4$ supplementation. Higher dose of Cu showed better result than lower dose.

• Asian-Australasian Journal of Animal Sciences
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• 제28권8호
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• pp.1140-1149
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• 2015

This research aimed to define the energy requirement of Dorper and Hu Hybrid $F_1$ ewes 20 to 50 kg of body weight, furthermore to study energy requirement changes with age and evaluate the effect of age on energy requirement parameters. In comparative slaughter trial, thirty animals were divided into three dry matter intake treatments (ad libitum, n = 18; low restricted, n = 6; high restricted, n = 6), and were all slaughtered as baseline, intermediate, and final slaughter groups, to calculate body chemical components and energy retained. In digestibility trial, twelve ewes were housed in individual metabolic cages and randomly assigned to three feeding treatments in accordance with the design of a comparative slaughter trial, to evaluate dietary energetic values at different feed intake levels. The combined data indicated that, with increasing age, the net energy requirement for maintenance ($NE_m$) decreased from $260.62{\pm}13.21$ to $250.61{\pm}11.79kJ/kg^{0.75}$ of shrunk body weight (SBW)/d, and metabolizable energy requirement for maintenance (MEm) decreased from $401.99{\pm}20.31$ to $371.23{\pm}17.47kJ/kg^{0.75}$ of SBW/d. Partial efficiency of ME utilization for maintenance ($k_m$, 0.65 vs 0.68) and growth ($k_g$, 0.42 vs 0.41) did not differ (p>0.05) due to age; At the similar condition of average daily gain, net energy requirements for growth ($NE_g$) and metabolizable energy requirements for growth ($ME_g$) for ewes during late fattening period were 23% and 25% greater than corresponding values of ewes during early fattening period. In conclusion, the effect of age upon energy requirement parameters in the present study were similar in tendency with previous recommendations, values of energy requirement for growth ($NE_g$ and $ME_g$) for Dorper and Hu crossbred female lambs ranged between the NRC (2007) recommendation for early and later maturating growing sheep.

• 보존과학회지
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• 제35권6호
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• pp.612-620
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• 2019

서울공예박물관에 소장된 모담방장(毛毯房帳)은 경사에 면사를 두고 위사에 모사를 두어 평직(平織)으로 짠 것이다. 문양 부분은 타피스트리(Tapestry)로 제직하고, 안료를 덧칠하였다. 색도를 측정하고, X선 형광분석에 의한 성분 분석 결과 진한 붉은 색의 문양에서는 수은(Hg)이 검출되어 주사(Cinnabar)나 합성안료인 주(Vermilion)로 추정하였다. 직물의 경사와 위사, 선단 및 후면 직물 등 총 7개의 직물 시료에 대한 분석을 실시하였다. 그 결과 모담방장의 경사는 섬유단면에 가운데 중공(루멘)이 있는 면섬유로, Infrared 피크도 마찬가지로 O-H 결합과 C-O 결합이 나타난다. 위사인 모섬유는 원형과 타원형의 단면이 확인되며, Infrared 피크는 N-H/O-H Stretching, Amide(-CONH-)을 보였다. 모섬유에 사용된 동물종을 확인하기 위해 문화재청의 동물털 시료와 대조분석 결과, 산양이나 염소털을 사용한 것으로 추정되며, 방적에 용이한 삽살개 털도 사용했을 가능성도 배제할 수 없다.