• Title/Summary/Keyword: hepatoprotective

Search Result 638, Processing Time 0.03 seconds

Modification of C2,3,23,28 Functional Groups on Asiatic Acid and Evaluation of Hepatoprotective Effects

  • Jeong, Byeong-Seon;Kim, Young-Choong;Lee, Eung-Seok
    • Bulletin of the Korean Chemical Society
    • /
    • v.28 no.6
    • /
    • pp.977-982
    • /
    • 2007
  • For the development of novel hepatoprotective agents, C2, C3, C23 and C28 functional groups on asiatic acid were modified, and the prepared compounds were evaluated for their hepatoprotective effects. Among the prepared compounds, 9, 13 and 16 showed significant hepatoprotective activities against CCl4- and galactosamine (GaIN)-induced hepatotoxicity. Especially, compound 9 showed the most significant hepatoprotective effects against GaIN-induced hepatotoxicity (66.4% protection at 50 μM) and moderate hepatoprotective activities against CCl4-induced hepatotoxicity (20.7% protection at 50 μM).

Antioxidative and Hepatoprotective Effects of Injinho-Tang and Osumogwa-Tang (인진호탕(茵蔯蒿湯)과 오수목과탕(吳茱木瓜湯)의 항산화(抗酸化) 및 간세포(肝細胞) 보호효과(保護效果))

  • Lee, Sang-Hyun;Kim, Young-Bok
    • Herbal Formula Science
    • /
    • v.16 no.1
    • /
    • pp.117-130
    • /
    • 2008
  • Korean traditional medicine has been used for the treatment of the various diseases based on both oriental medicinal theory and clinical trials. Thus, the prescriptions of Korean traditional medicine would be useful for the development of new therapeutics. This research focuses on the fundamental study in Korean traditional prescriptions for the development of new hepatoprotective agents. We found two prescriptions. Injinho-Tang and Osumogwa-Tang, showed the significant DPPH free radical scavenging and hepatoprotective effect, respectively. It is well-known that free radical scavenging effect is related to the prevention of various pathological events including liver injury. This paper deals with hepatoprotective effects on tacrine-induced cytotoxicity in Hep G2 cells, free radicals scavenging on both DPPH and superoxide of above two prescriptions. Hot water extract of Injinho-Tang did not show the significant hepatoprotective effect on tacrine-induced cytotoxicity in Hep G2 cells, however, it shows the significant scavenging effects for both DPPH and superoxide radicals. On the other hand, all of the hot water extracts of constituent herbal drugs in Injinho-Tang exhibited the promising protective effect on tacrine-induced cytotoxicity in Hep G2 cells. Of these, water extract of Rhei Rhizoma showed the most prominent effect on tacrine-induced cytotoxicity in Hep G2 cells. Bioassay-guided fractionation of Rhei Rhizoma extract has furnished four compounds, and their chemical structures have been identified by comparison of their spectral data with those of literature as chrysophanol (1), emodin (2), 3,5-dihydroxy-4'- methoxystilbene (3), and rhapontigenin (4), respectively. Among the isolated compounds, compounds 2-4 revealed the significant hepatoprotective effect in vitro when their $EC_{50}$ values compare with that of silybin, as a positive control. It also exhibited that emodin possessed the most hepatoprotective effect among these active compounds. In case of Osumogwa-Tang, its hot water extract showed the moderate protective effect on tacrine-induced cytotoxicity in Hep G2 cells. Hot water extract of Chaenomelis Fructus, one of the constituent herbal drug of this prescription, exhibited the significant hepatoprotective effect with $EC_{50}$ value of $7.8{\pm}0.1\;{\mu}g/ml$, however, it showed strong cytotoxicity in Hep G2 cells above the concentration of $25\;{\mu}g/ml$. It was revealed that both hot water extract of Evodiae Fructus and its butanol soluble fraction showed the moderate hepatoprotective effect but concentration-dependent activity in Hep G2 assay system. Two quinolone alkaloids, evocarpine and dihydroevocarpine, also tested for their hepatoprotective effects on tacrine-induced cytotoxicity in Hep G2 cells, however, these two compounds derived from the Evodiae Fructus as the major constituents did not show in vitro hepatoprotective effect. From these results, it would be necessary to further isolation of its hepatoprotective compounds from the butanol soluble fraction of the hot water extract of Evodiae Fructus.

  • PDF

Free Radical Scavenging and Hepatoprotective Effects of Osumokkya-tang (오수목과탕(吳茱木瓜湯)의 자유(自由)라디칼 소거활성(演去活性) 및 肝(간) 세포(細胞) 보호효과(保護效果))

  • Jeong, Ok-Sam;Yun, Young-Gab;Kim, Youn-Chul
    • Herbal Formula Science
    • /
    • v.13 no.2
    • /
    • pp.93-100
    • /
    • 2005
  • In order to find the new hepatoprotective agents preliminary screening of twelve prescriptions which have been used for the treatment of hepatic disease in Oriental traditional medicine has been carried out. Both DPPH free radical scavenging and hepatoprotective effect on tacrine-induced cytotoxicity in Hep G2 cells are performed. Osumokkya-tang which is one of the prescription belongs to interior-warming properties showed the DPPH free radical scavenging effect as well as hepatoprotective activity significantly. It is also suggested that its hepatoprotective effect is derived from Chaenomelis Fructus.

  • PDF

Hepatoprotective Effects of Polysaccharides of Croton tiglium on $\textrm{CCl}_4$ Intoxication (사염화탄소의 간독성에 대한 파두 다당류분획의 예방효과)

  • 이은경;길이룡;소동수;창동신;전선덕;정명규;문창규
    • Environmental Analysis Health and Toxicology
    • /
    • v.11 no.3_4
    • /
    • pp.59-63
    • /
    • 1996
  • In present study, we fractionated polysaccharides from Croton tiglium and investigated their hepatoprotective effects on CCl$_4$ intoxication. Polysaccharide fraction of which molecular weight is over 300,000 (HP) showed the most potent hepatoprotective effects on CCl$_4$ intoxication. Lipid peroxidation, sAST and sALT were used as parameters to evaluate the liver damage. Glucose, xylose and arabinose were found to be monosaccharides composing sugar moiety of HP.

  • PDF

Synthesis of Aryloxyallylthiopyridazine Derivatives (Aryloxyallylthiopyridazine 유도체 합성)

  • 권순경;김미경
    • YAKHAK HOEJI
    • /
    • v.46 no.2
    • /
    • pp.89-92
    • /
    • 2002
  • Allylthio group of the sulfur compounds of garlic oil plays an important role for prevention and treatment of hepatic diseases induced by toxic substances or carbon tetrachloride. Thus allylthio group as pharmacologically active group was introduced into pyridazine heterocycle ring. Aryloxyallylthiopyridazine derivatives were synthesized and their hepatoprotective activities were screened in rat. The activities of these compounds were weaker than alkoxyallylthiopyridazine derivatives, which exhibit a superior hepatoprotective effect.

Hepatoprotective Constituents of Cudrania tricuspidata

  • Tian Yu-Hua;Kim Hyun-Chul;Cui Jiong-Mo;Kim Youn-Chul
    • Archives of Pharmacal Research
    • /
    • v.28 no.1
    • /
    • pp.44-48
    • /
    • 2005
  • Phytochemical investigation of the MeOH extract of the root barks of Cudrania tricuspidata Bureau (Moraceae), as guided by hepatoprotective activity in vitro, furnished four isoprenylated xanthones, cudratricusxanthone A (1), cudraxanthone L (2), cudratricusxanthone E (3), and macluraxanthone B (4). All of these compounds showed the significant hepatoprotective effect on tacrine-induced cytotoxicity in human liver-derived Hep G2 cells. Compounds 1, 2, and 4 also exhibited the significant hepatoprotective effect on nitrofurantoin-induced cytotoxicity in human liver-derived Hep G2 cells.

In Vitro Free Radical Scavenging and Hepatoprotective Activities of Taraxacum mongolicum (포공영의 자유라디칼 소거 및 간세포 보호활성)

  • Baek, Hum-Young
    • Korean Journal of Pharmacognosy
    • /
    • v.34 no.4 s.135
    • /
    • pp.324-326
    • /
    • 2003
  • The methanol (MeOH) extract and ist fractions of Taraxacum mongolicum (Compositae) were examined for their scavenging effects on 1,1-diphenyl-2- phenylhydrazyl (DPPH) and superoxide radicals, and hepatoprotective effects on tacrine-induced cytotoxicity in human hepatoma cell line, Hep G2 cells. Both methylene chloride $(CH_2Cl_2)$ and butanol (n-BuOH) soluble fractions of the MeOH extract showed the free radicals scavenging and hepatoprotective effects. From these results, it is suggested that hepatoprotective effect of these fractions partly relies on their free radical scavenging activity.

Hepatoprotective Activities of Biologically Active Agents from Crude Drugs(I) -Hepatoprotective Activities of Traditional Korean Herbal Prescriptions- (식물성생리활성물질(植物性生理活性物質)의 간보호작용(肝保護作用)(I) -한약처방(韓藥處方)의 간보호작용(肝保護作用)-)

  • Kim, Chang-Johng;Cho, Seung-Kil;Choi, Chung-Sik;Choi, Yun-Suk
    • Korean Journal of Pharmacognosy
    • /
    • v.21 no.3
    • /
    • pp.223-234
    • /
    • 1990
  • Hepatoprotective activities of 26 traditional Korean herbal prescriptions described in Donghibogam were evaluated in vivo and in vitro screening system. Twenty one prescriptions appeared to be active. Six prescriptions among them showed highly significant hepatoprotective activities; Shihogyegy-Tang(柴胡桂枝湯), Soshiho-Tang(小柴胡湯), Shiho plus Younggolmoryo-Tang(柴胡加龍骨牡蠣湯), Yinjinho-Tang(茵蔯蒿湯), Yinsam-Tang(人蔘湯) and Shashim-Tang(瀉心湯). Shihogyegy-Tang was most active. They reduced the release of transaminases from dissociated hepatocytes by thioacetamide, and serum transaminases and alkaline phosphatase activities of rat with carbontetrachloride-induced hepatitis. They inhibited significantly lipid peroxidation and cellular fatty change of liver by carbontetrachloride.

  • PDF

Hepatoprotective Effect and Antioxidant Role of Caesalpinia bonducella on Paracetamol-induced Hepatic Damage in Rats

  • Gupta, Malaya;Mazumder, Upal Kanti;Kumar, Ramanathan Sambath
    • Natural Product Sciences
    • /
    • v.9 no.3
    • /
    • pp.186-191
    • /
    • 2003
  • The hepatoprotective effect of methanol extract of leaves of Caesalpinia bonducella was studied by means of paracetamol induced liver damage in rats. The degree of protection was measured by using biochemical parameters such as serum transaminase (SGPT and SGOT), alkaline phosphatase (ALP), bilirubin, and total protein. Further, the effects of the extract on lipid peroxidation (LPO), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) were estimated. The methanol extract of C. bonducella (MECB) (50,100 and 200 mg/kg) produced significant (P<0.01) hepatoprotective effect by decreasing the activity of serum enzymes, bilirubin, and lipid peroxidation, while it significantly increased increased the levels of GSH, SOD, CAT, and protein in a dose dependent manner. The effects of MECB were comparable to that of standard drug Silymarin. However, at a lower dose (25 mg/kg) it could not restore the deleterious effect produced by paracetamol. The results indicate that Caesalpinia bonducella had antioxidant and hepatoprotective effects.

Hepatoprotective Effect of Subfractions of Carthamus tinctorius L. Semen on the Reversal of Biotransformation Enzyme Activities in CCl4-induced Hepatotoxic Rats (사염화탄소로 유발된 간손상에서의 효소 활성도의 변화로 본 홍화자 분획물의 간손상 보호 작용)

  • 정춘식;정기화;정정숙
    • Journal of Food Hygiene and Safety
    • /
    • v.14 no.2
    • /
    • pp.172-178
    • /
    • 1999
  • Previous studies have shown that methanol extract and its butanol fraction of Carthamus tinctorius L. Semen have the hepatoprotective effect on the CCl4-induced hepatotoxicity. The hepatoprotective effect of subfractions has been evaluated by analyzing blood and hepatocyte biochemical analyses and biotransformation enzyme analyses. Treatment of BS-5, subfraction has significantly decreased the activities of alanine aminotransferase and aspartate aminotransferase. In addition, the levels of cholesterol and triglyceride in liver have been decreased as compared with that of CCl4 treated rats. The hepatoprotective effect of BS-5, subfraction on the CCl4-induced hepatotoxicity would be mediated of the attenuation of the level of cytochrome P450 and the enhancement of the activity of glutathion S-transferase.

  • PDF