• Title, Summary, Keyword: hepatoprotective

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In Vitro Free Radical Scavenging and Hepatoprotective Activities of Taraxacum mongolicum (포공영의 자유라디칼 소거 및 간세포 보호활성)

  • Baek, Hum-Young
    • Korean Journal of Pharmacognosy
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    • v.34 no.4
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    • pp.324-326
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    • 2003
  • The methanol (MeOH) extract and ist fractions of Taraxacum mongolicum (Compositae) were examined for their scavenging effects on 1,1-diphenyl-2- phenylhydrazyl (DPPH) and superoxide radicals, and hepatoprotective effects on tacrine-induced cytotoxicity in human hepatoma cell line, Hep G2 cells. Both methylene chloride $(CH_2Cl_2)$ and butanol (n-BuOH) soluble fractions of the MeOH extract showed the free radicals scavenging and hepatoprotective effects. From these results, it is suggested that hepatoprotective effect of these fractions partly relies on their free radical scavenging activity.

Antioxidant and Hepatoprotective Effects of Amomum Xanthoides

  • Wang, Jing-Hua;Shin, Jang-Woo;Son, Chang-Gue
    • The Journal of Korean Medicine
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    • v.31 no.3
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    • pp.98-106
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    • 2010
  • Objective: To examine the in vitro antioxidant and hepatoprotective properties of Amomum xanthoides extract and its fractions. Methods: Methanol extract of Amomum xanthoides was fractionated into three fractions; petroleum ether fraction (PFAX), water fraction (WFAX) and ethyl acetate fraction (AFAX). The antioxidant and hepatoprotective effects of these fractions were compared by in vitro experiments. Results: The total phenols content and DPPH (1 1-Diphenyl-2-picrylhydrazyl) radical scavenging activity was found significant in AFAX when compared with other fractions (PFAX, WFAX). AFAX significantly inhibited NO production by RAW 264.7 cells in LPS-activated condition. In Hep $G_2$ cells, AFAX markedly prevented the leakage of LDH and release of AST against CCl4 challenge. Conclusion: Amomum xanthoides and especially ethyl acetate fraction have potent antioxidative and hepatoprotective properties.

An Isocoumarin with Hepatoprotective Activity in Hep G2 and Primary Hepatocytes from Agrimonia pilosa

  • Ko, Eun-Kyung;Park, Eun-Jeon;Kim, Mi-Hee;Jun, Jung-Yang;Park, Sung-Uk;Sohn, Dong-Hwan;Kim, Youn-Chul
    • Proceedings of the PSK Conference
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    • pp.383.1-383
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    • 2002
  • In connection with our studies on the isolation of hepatoprotective constituents from natural products. we have recently reported hepatoprotective compounds including phenolic bakuchiol. diarylheptanoids. furocoumarins. In the course of continuing efforts. the aqueous extract of the roots of Agrimonia pilosa Ledeb. (Rosaceae) was found to exhibit promising hepatoprotective activity. A. pilosa is a perennial herb distributed throughout South Korea. and its roots have been used as the hemostatic. antimalarial. and antidysenteric agent in oriental medicine. Chemical investigation of the aqueous extract of the roots of this plant. as guided by hepatoprotective active catechin (2). Compound 1 showed hepatoprotective effects on both tacrine-induced cytotoxicity in human level derived Hep G2 cells and tert-hydroperoxide-induced cytotoxicity in rat primary hepatocyles with $EC_{50}$ values of 66.2 $\pm$ 2.8 and 22.9 $\pm$ 2.6 $\mu\textrm{M}$ respectively.

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Hepatoprotective Lignan Compound from Schizandrae Fructus on Tacrine-induced Cytotoxicity in Hep G2 Cells

  • Kim, Hyun-Chul;An, Ren-Bo;Jeong, Gil-Saeng;Bum, Jin-Sun;Oh, Seung-Hwan;Lee, Hye-Suk;Kwon, Ji-Wung;Kwon, Tae-Oh;Kim, Youn-Chul
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.20 no.5
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    • pp.1282-1284
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    • 2006
  • In the course of search for hepatoprotective agents from oriental traditional medicines, one compound was isolated from the MeOH extract of the fruits of Schizandra chinensis, and its structure was determined as gomisin J(1). The evaluation for hepatoprotective activity of isolated compound 1 on drug-induced cytotoxicity was conducted, and compound 1 showed protective effect with an EC$_{50}$value of 86.0 ${\pm}$ 5.3 ${\mu}$M against tacrine-induced cytotoxicity in Hep G2 cells. One of the well-known hepatoprotective agents, silybin, used as a positive control, and the hepatoprotective effect of compound 1 is similar with that of silybin (EC$_{50}$ value = 90.5 ${\pm}$ 9.8 ${\mu}$M).

Hepatoprotective Effect of Lactic Acid Bacteria, Inhibitors of $\beta$-Glucuronidase Production Against Intestinal Microflora

  • Han Song Yi;Huh Chul Sung;Ahn Young Tae;Lim Kwang Sei;Baek Young Jin;Kim Dong Hyun
    • Archives of Pharmacal Research
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    • v.28 no.3
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    • pp.325-329
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    • 2005
  • The hepatoprotective activity of lactic acid bacteria (Lactobacillus brevis HY7401, Lactobacillus acidophilus CSG and Bifidobacterium longum HY8001), which inhibited $\beta$-glucuronidase productivity of intestinal microflora, on t-BHP- or CCl$_4$-induced hepatotoxicity of mice were evaluated. These oral administration of lactic acid bacteria lowered $\beta$-glucuronidase production of intestinal microflora as well as Escherichia coli HGU-3. When lactic acid bacteria at a dose of 0.5 or 2 g (wet weight)/kg was orally administered on CCl$_4$-induced liver injury in mice, these bacteria significantly inhibited the increase of plasma alanine transferase and aspartate transferase activities by $17-57\%$ and $57-66\%$ of the $CCI_4$ control group, respectively. These lactic acid bacteria also showed the potent hepatoprotective effect against t-BHP-induced liver injury in mice. The inhibitory effects of these lactic acid bacteria were more potent than that of dimethyl diphenyl bicarboxylate (DDB), which have been used as a commercial hepatoprotective agent. Among these lactic acid bacteria, L. acidophilus CSG exhibited the most potent hepatoprotective effect. Based on these findings, we insist that an inhibitor of $\beta$-glucuronidase production in intestine, such as lactic acid bacteria, may be hepatoprotective.

Hepatoprotective Constituents of the Edible Brown Alga Ecklonia stolonifera on Tacrine-induced Cytotoxicity in Hep G2 Cells

  • Kim, Youn-Chul;An, Ren-Bo;Yoon, Na-Young;Nam, Taek-Jeong;Choi, Jae-Sue
    • Archives of Pharmacal Research
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    • v.28 no.12
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    • pp.1376-1380
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    • 2005
  • In this study, ethanolic extracts from 18 seaweed variants were assessed for hepatoprotective activity against tacrine-induced cytotoxicity in Hep G2 cells. Only one of these, Ecklonia stolonifera Okamura (Laminariaceae), a member of the brown algae, exhibited promising hepatoprotective activity. Bioassay-guided fractionation of the active ethyl acetate (EtOAc) soluble fraction obtained from the ethanolic extract of E. stolonifera, resulted in the isolation of several phlorotannins [phloroglucinol (1), eckstolonol (2), eckol (3), phlorofucofuroeckol A (4), and dieckol (5)]. Compounds 2 and 4 were determined to protect Hep G2 cells against the cytotoxic effects of tacrine, with $EC_{50}$ values of 62.0 and 79.2 $\mu$g/mL, respectively. Silybin, a well characterized hepatoprotective agent, was used as a positive control, and exhibited an $EC_{50}$ value of 50.0 $\mu$g/mL. It has been suggested that the phlorotannins derived from marine brown algae might prove useful sources in the development of novel hepatoprotective agents.

Curcuma Extract Enhances Hepatoprotective Action of Silymarin in Experimental Liver Damage Models (실리마린의 간보호 작용에 미치는 쿠쿠마 추출물의 영향)

  • 이기명;함기백;조성원;오태영;최설민;김정훈;안병옥;권종원;김원배
    • Biomolecules & Therapeutics
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    • v.10 no.1
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    • pp.12-18
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    • 2002
  • Silymarin and curcumin have been used for supportive treatment of liver disease of difffrent etiology due to their hepatoprotective activities. The present study was carried out to investigate the hepatoprotective efffcts of silymarin and/or curcuma extract against hepatotoxins induced liver injury. To investigate hepatoprotective effects, the silymarin and/or curcuma extract were pre-treated orally to experimental animals. And thereafter a single dose of hepatotoxin, carbon tetrachloride ($CCl_4$) and acetaminophen were administered through oral or intraperitoneal route, respectively. Chronic liver damage was induced by subcutaneous injection of $CCl_4$ for 3 weeks (2 times/week). Hepatoprotective and therapeutic effects were monitored by estimating serurn ALT and AST levels and by measuring hepatic glutathione (GSH) and malondialdehyde (MDA)levels. Collagen type 1 was detected with irnrnunostaining to assess fibrosis. The results showed that the mix-ture of silymarin and curcuma extract significantly reduced serum biochemistry levels and MDA levels com-pared with those of control group in both acute and chronic animal models. In antifibrotic effect, the relative hepatic collagen content was significantly decreased by silymarin and/or curcuma extract treatment. It was concluded that the complex of silymarin and curcuma extract have a both hepatoprotective and therapeutic effect synergically in rat liver injury induced by heptotoxins.

Hepatoprotective Activity of Chungpesagan-tang is Related to the Inhibition of ${\beta}-Glucuronidase$

  • Kim, Jae-Kwan;Bae, Hyung-Sup;Kim, Young-Suk;Cho, Ki-Ho;Lee, Kyung-Sup;Lee, Hae-Ung;Kim, Dong-Hyun
    • Natural Product Sciences
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    • v.7 no.3
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    • pp.63-67
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    • 2001
  • ${\beta}-Glucuronidase-inhibitory$ and hepatoprotective effects of Chungpesagan-tang, which has been used for liver diseases and stroke, on $CCl_4-induced$ hepatotoxicity of rats was investigated. Chungpesagan-tang potently inhibited ${\beta}-glucuronidases$. Serum AST, ALT and LDH levels of the $CCl_4$ group orally treated with Chungpesagan-tang (100 mg/kg) were lowered to 47, 28 and 58% of the $CCl_4-treated$ group, respectively. Among the ingredients of Chungpesagan-tang, Puerariae Radix, Scutellariae Radix and Rhei Rhizoma potently inhibited the ${\beta}-glucuronidase$ and protected $CCl_4-induced$ liver injury. The hepatoprotective activity of Puerariae Radix was affected by ingredients of Chungpesagan-tang: Scutellariae Radix had the synergistic activity, but Angelicae Tenussimae Radix exhibited the antagonistic activity. These results suggest that the ${\beta}-glucuronidase$ inhibitor of herbal medicines may protect $CCl_4-induced$ liver injury and puerarin should be a natural prodrug for the hepatoprotective effect.

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Screening of Medicinal Plants Having Hepatoprotective Activity Effects with Primary Cultured Hepatocytes Intoxicated Using Carbon tetrachloride Cytotoxicity ($CCl_4$로 독성유발시킨 초대배양 간세포를 이용하여 간세포 보호효과를 나타내는 생약류의 검색)

  • Lee, June-Woo;Choi, Joon-Han;Kang, Sang-Mo
    • Korean Journal of Pharmacognosy
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    • v.23 no.4
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    • pp.268-275
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    • 1992
  • We studied to screen medicinal plants having hepatoprotective activity with the primary cultured rat hepatocytes intoxicated with carbon tetrachloride cytotoxicity. The lowest concentration and treatment time of carbon tetrachloride giving the greatest intoxication to the primary cultured hepatocytes were observed in 10mM and 60 minutes, respectively. GTP and GOT activity of culture broth of the primary cultured rat hepatocytes intoxicated by $CCl_4$ cytotoxicity at this condition were increased 135.9% and 178.3% compared with that of the primaries cultured hepatocytes not treated with $CCl_4$, respectively. This increased GPT activity was inhibited by glycyrrizin, which was known to have hepatoprotective activity, and the inhibition activity was dependent on the concentration of glycyrrhizin. Forty species among the extracts obtained from 117 species of medicinal plants were shown to have the hepatoprotective activity. Among these 40 species, Prunus persica, Scutellaria baicalensis, Astragalus membranaceus, Tribulus terrestris, Caragana chamlagu, Acanthopanax sessiliflorum and Achyranthes japonica were indicated a lower GPT activity than that of Glycyrrhiza uralensis containing glycyrrhizin and GPT activity of these were indicated 75.5%, 70.0%, 59.0%, 77.5%, 60.0%, 75.0% and 79.0%, respectively.

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Hepatoprotective Effects of Poly Herbal Formulation (Hepa-1000) on t-BHP Induced Toxicity in Human Hepatoma Cells (간기능 개선용 복합 식물 추출물(Hepa-1000)의 tert-butyl hydroperoxide(t-BHP)로 유도한 간세포 독성에 대한 보호 효과)

  • Lee, Eu-Gene;Kim, Kyung-Bum;Jeong, Jong-Moon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.9
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    • pp.1121-1126
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    • 2006
  • In the present study, the potential hepatoprotective effects of poly herbal formulation, Hepa-1000, against oxidative damages induced by t-BHP were evaluated in HepG2 cells in order to relate in vitro antioxidant activity with cytoprotective effects. The t-BHP induced considerable cell damage in HepG2 cells was shown by significant glutamic oxaloacetic transaminase (GOT) and lactate dehydrogenase (LDH) leakage, and increased lipid peroxidation. Hepa-1000-treated cells showed an increased resistance to oxidative challenge, as revealed by higher survival capacity than the one of control cells against t-BHP induced oxidative stress and hepatotoxicity. In addition, the Hepa-1000 had hepatoprotective effects lowering the activity of GOT and LDH, simultaneously. That is, it could inhibit the cell membrane damages resulting in the increased activities of GOT and LDH in the cell culture media. Furthermore, the Hepa-1000 could reduce t-BHP enhanced lipid peroxidation, which was evaluated by measuring the production of malonedialdehyde. Based on the data described above, it could be suggested that the Hepa-1000 has significant hepatoprotective effects and plays a protective role against lipid peroxidation by free radicals.