• Preventive Nutrition and Food Science
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• 제14권4호
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• pp.373-377
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• 2009

Safflower seed extract was prepared by a high-pressure extraction technology and its quality characteristics were compared to that of other conventional extraction techniques, such ultrasonic and reflux extractions. Safflower seeds were extracted with 80% aqueous ethanol by three above extraction methods, and further fractionated with Diaion HP-20 column chromatography to obtain a partially purified safflower seed extract (PPSSE). Among the three extraction techniques examined, the reflux extraction showed the higher yields of EtOH extract and PPE than the ultrasonic and high-pressure extractions. Levels of most phenolic compounds in the EtOH extract of safflower seed are higher in reflux and ultrasonic extractions than the high pressure extraction, but levels of two serotonin aglycones, N-(p-coumaroyl)serotonin (CS) and N-feruloylserotonin (FS), in PPSSE were higher in the high pressure extraction than the reflux and ultrasonic extractions. In addition, color values (L and a) of the PPSSE were higher in the high-pressure extraction than the reflux and ultrasonic extractions, although there were no significant differences in pH and UV maxima absorption spectra among three extraction techniques. These results indicate that the high-pressure extraction technology is a simple and effective extraction for preparation of a high quality of safflower seed extract containing CS and FS with anti-wrinkle activity.

• 한국식생활문화학회지
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• 제23권6호
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• pp.667-680
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• 2008

The moisture contained in conventionally extracted red crab shell samples was lower than than in samples extracted using high pressure. In contrast, ash content increased as the extraction time increased and displayed no significant variation in high pressure extraction. Extraction time was influential, with lower lightness (L-value) being obtained in samples extracted at high pressure as compared to traditional extraction. However, large variation in a- and b-values resulted from traditional extraction. For both traditional and high pressure extraction increases in pH, salinity and saccharide content were noted with increasing extraction time, but these parameters did not vary substantially in red crab shell extracted at high pressure. The mineral content in samples extracted by high pressure was higher than in traditionally extracted samples. The mineral content increased with time, with marginally higher contents of essential amino acids and flavor enhancing amino acids noted following high pressure extraction. The contents of the flavor enhancing amino acids increased from 30?60 min, thereafter decreasing. Assessment of consumer acceptance revealed that, while traditionally manufactured red crab sauce was popular, sauce manufactured using a 60 min high pressure extraction was preferred. Extending the high pressure extraction time to 75 min produced a less sensory acceptable product, due to higher mineral and protein levels.

• 한국약용작물학회지
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• 제19권2호
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• pp.103-110
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• 2011

This study was performed to enhance anticancer activities of Lithospermum erythrorhizon by eluting high amount of shikonin through ultra high pressure process. Extraction yield was increased up to 5~10% by ultra high pressure process, compare to the normal extraction processes such as water solvent extraction, 70% ethyl alcohol solvent extraction. The cytotoxicity of the extracts ($1.0{\mu}g/m{\ell}$) from ultra high pressure process was showed the lowest cytotoxicity 13.4% for human lung cell (HEL299). The anticancer activities showed 80~85% by adding $1.0{\mu}g/m{\ell}$ of the extracts from ultra high pressure process in several cancer cell lines such as AGS, Hep3B, MCF-7 and HeLa cells. Among them, MCF-7 cell of the endocrine system was highest inhibited than other cells. The anticancer activities of the extracts from ultra high pressure extraction process showed 10~15%, which was higher than the extracts from normal extraction processes. From HPLC analysis of the extracts, the contents of shikonin in the extracts from ultra high pressure process was 11.42% (w/w), which was 20% higher than others. This results indicate that ultra high pressure process could increase the extraction yield of shikonin and other contents, which resulted in higher anticancer activities.

• 한국약용작물학회지
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• 제22권2호
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• pp.121-126
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• 2014

This study was designed to evaluate antioxidant activity of Curcuma longa L. leaves treated by ultra high pressure extraction. Curcuma longa L. leaves was subjected to 5,000 bar for 5 and 15 min at $25^{\circ}C$ The highest phenolics and flavonoids content was observed in the treatment of high pressure extraction for 15 min ($308.28{\mu}g/mg$, $124.33{\mu}g/mg$). The DPPH scavenging activity was 82.34% at $1.0mg/m{\ell}$ of Curcuma longa L. leaves treated by ultra high pressure process for 15 min. The highest SOD-like acitivity of Curcuma longa L. leaves ($1.0mg/m{\ell}$) was observed at ultra high pressure extraction for 15 min (67.54%). The high pressure extraction significantly increased the contents of phenolics and flavonoids and also enhanced the antioxidant activity. These results provide useful information for enhancing biological properties of Curcuma longa L. leaves.

• Preventive Nutrition and Food Science
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• 제13권4호
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• pp.321-326
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• 2008

In order to improve the market competitiveness of the processed products of Undaria pinnatifida, various extraction conditions of Undaria pinnatifida were examined to determine the optimal conditions for the extraction of its valuable components. The highest level of alginic acid content was detected after 90 min of typical extraction or after 20 min of pressure extraction, after which the levels decreased slightly. The concentrations of reducing sugar and crude protein were also high after 90 min of typical extraction. Both alginic acid and reducing sugar were extracted in greater amounts using pressure extraction. The reducing sugar content was $2.8{\sim}3.2$ times higher using pressure extraction rather than typical extraction. Furthermore, the extraction results were superior with the, pressure extraction method. The appropriate temperature and duration of extraction were found to be $120^{\circ}C$ for 20 min. The use of a single enzyme yielded better results during extraction compared to the use of a mixture of enzymes. 20 min of pressure extraction followed by the addition of 0.1% pectinase and 2 hr of further extraction at $50^{\circ}C$ yielded high contents of alginic acid and reducing sugar from Undaria pinnatifida.

• 한국조리학회지
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• 제20권3호
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• pp.13-21
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• 2014

본 연구에서는 순환형 감압건조 및 초고압 추출공정을 이용하여 전통적인 기존 추출공정과 비교함으로써 복합 추출공정에 의한 감귤의 항산화 활성 증진을 확인하고자 연구를 수행하였다. 건조공정을 거친 후 초고압 처리 추출물의 수율이 20.41~28.19%로 높은 추출 수율을 나타내어 건조전 열수추출공정(17.21%)과 비교하여 약 1.6배의 높은 추출수율을 나타내었다. 순환형 감압건조와 초고압 공정을 병행하였을 시 총 폴리페놀과 플라보노이드 함량이 순환형 감압건조공정을 거치지 않은 것보다 다소 증가되는 것으로 보아 활성성분의 용출이 증진된 것으로 보인다. DPPH radical 소거 활성은 15분 초고압 처리한 추출물이 48.21%로 높은 활성을 나타내었다. 전처리 공정에 따른 감귤 시료의 주사전자현미경(SEM)을 통해 순환형 감압건조 후 초고압 추출이 감귤 내부 조직까지 영향을 주어 세포벽이 깨어지면서 조직 및 구조가 변화한 것으로 판단되며, 이를 통해 수율 및 활성 성분의 용출 증가가 이루어 진 것으로 사료된다. 따라서, 감귤의 건조 및 초고압 추출공정의 최적화를 통한 활성물질의 추출 극대화를 통해 추출수율을 향상시킬 것으로 판단된다.

• 한국조리학회지
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• 제14권3호
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• pp.210-222
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• 2008

This study showed that the brown stock, which is the base of demi-glace sauce, extracted by using a high pressure heating extractor is more advantageous than that extracted by the traditional extraction method for the mass production. We compared the former with the latter in terms of minerals and free amino acids. The results of this study are summarized as follows. When mineral contents were compared, the brown stock extracted by high.pressure heating extraction showed the tendency of increase in mineral contents in proportion to heating temperature and heating time, but, from extraction temperature of 140$^{\circ}C$, the contents of K, Mg, Na and P decreased with the increase of extraction time. In addition, mineral contents in the brown stock extracted by high-pressure heating extraction were generally lower than those in brown stock extracted by the traditional extraction method. This result was produced probably because materials were added repeatedly in the traditional method. Amino acids contents in brown stock according to the extraction methods were also examined. They increased with the increase in the number of extractions in the brown stock extracted by the traditional method, and those in the brown stock extracted using a high pressure heating extractor increased with the increase in heating temperature and extraction time. The results of this study are expected to be useful as a practical material for the mass production of brown stock products.

• 한국조리학회지
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• 제20권4호
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• pp.27-36
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• 2014

본 연구에서는 초고압 추출공정을 이용하여 전통적인 기존 추출공정과 비교함으로써 초고압 추출공정에 의한 꼬마칼라감자의 항산화 활성 증진을 확인하고자 연구를 수행하였다. 초고압 처리 추출물의 수율이 15분 처리시 2.10, 30분 처리시 2.41%로 추출 수율을 나타내어 일반 열수추출공정(1.73%)과 비교하여 약 1.2~1.4배의 추출수율을 나타내었다. 초고압 공정에 따른 변화 비교에서는 초고압 공정을 병행하였을 시 총 페놀과 플라보노이드 함량이 초고압 공정을 거치지 않은 것보다 다소 증가되는 것으로 보아 활성성분의 용출이 증진된 것으로 보인다. DPPH radical 소거 활성은 30분 초고압 처리한 추출물이 1.0 mg/mL에서 76.21%로 높은 활성을 나타내었으며, 환원력 역시 전체적으로 초고압 공정을 실시하였을 때의 활성이 높게 측정되었다. 초고압 추출이 꼬마칼라감자 내부 조직까지 영향을 주어 세포벽이 깨어지면서 조직 및 구조가 변화한 것으로 판단되며, 이를 통해 수율 및 활성 성분의 용출 증가가 이루어 진 것으로 사료된다. 따라서 꼬마칼라감자의 초고압 추출공정의 최적화를 통한 활성물질의 추출 극대화를 통해 높은 경제적 가치를 이룰 것으로 판단된다.

• KSBB Journal
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• 제23권5호
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• pp.362-368
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• 2008

본고에서는 초고압 시스템 (High hydrostatic pressure (HHP))의 특성, 종류, 응용을 예로 들어 설명하였다. 초고압 기술은 식품의 살균, 식품 보존기간연장, 유효성분의 추출 등에 선택적으로 적합하게 적용하여 생물산업공정에 효율적으로 응용 할 수 있다. 초고압 시스템을 효율적으로 적용하기 위한 최적조건을 선정하는 것은 많은 조업 변수에 대한 고려를 해야 하는 힘든 작업이다. 따라서 하드웨어에 대한 기초 실험과 예비 생산을 통하여 공정에 대한 모사를 검증하고 생산 효율을 증가시킬 수 있다. 또한 초고압 시스템의 중요성이 증가함에 따라 고성능 및 사용이 더욱 편리한 시스템이 나타날 것이며, 많은 영역의 바이오산업에서 필수적으로 이용될 것이다.

• 한국식품영양과학회지
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• 제39권12호
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• pp.1898-1902
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• 2010

본 연구에서는 초고압 추출공정을 이용하여 전통적인 기존 추출공정과 비교함으로써 초고압 추출공정에 의한 등급 외 더덕 및 발효더덕의 항산화 활성의 증진을 확인하고자 연구를 수행하였다. 총 페놀화합물 함량은 초고압 발효더덕 추출물이 35.11 mg/100 g으로 초고압 더덕 추출물 14.78 mg/100 g, 일반더덕 추출물 14.56 mg/100 g에 비해 총 페놀화합물 함량이 약 2배 이상 높은 수치를 나타내었다. 또한, 플라보노이드 함량은 페놀화합물과 유사한 경향으로 초고압 발효더덕 추출물이 280.86 mg/100 g으로 초고압 더덕 추출물의 198.35 mg/100 g과 일반더덕 추출물 193.05 mg/100 g에 비해 약 1.4배 정도 높은 함량을 나타내었다. 항산화도는 초고압 발효더덕 추출물의 경우 0.6 mg/mL에서 약 70%의 항산화도를 나타내었으며, 이 값은 초고압 더덕 추출물 60.35%와 일반더덕 추출물 30.92%보다 높은 항산화도를 나타내었다. 초고압 발효더덕 추출물의 환원력은 최종농도 1.0 mg/mL에서 일반더덕의 경우 0.733, 초고압 더덕 추출물의 경우 0.881, 초고압 발효더덕 추출물의 경우 0.926 으로서 초고압 발효더덕 추출물의 환원력이 다른 추출물보다 약 0.193까지 높은 결과를 나타내었다. 따라서 초고압 추출공정이 등급 외 더덕 및 발효더덕의 기능성을 증가시킬 수 있으며, 초고압 추출공정의 최적화를 통한 활성물질의 추출 극대화를 통해 높은 경제적 가치를 부여해 줄 수 있을 것으로 사료된다.