• Asian Pacific Journal of Cancer Prevention
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• 제16권18호
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• pp.8253-8258
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• 2016

Elevated levels of soluble CD30 (sCD30) are linked with various T-cell neoplasms. However, the relationship between sCD30 levels and the development of adult T-cell leukemia (ATL) in human T-cell leukemia virus type 1 (HTLV-1) carriers remains to be clarified. We here investigated whether plasma sCD30 is associated with risk of ATL in a nested case-control study within a cohort of HTLV-1 carriers. We compared sCD30 levels between 11 cases (i.e., HTLV-1 carriers who later progressed to ATL) and 22 age-, sex- and institution-matched control HTLV-1 carriers (i.e., those with no progression). The sCD30 concentration at baseline was significantly higher in cases than in controls (median 65.8, range 27.2-134.5 U/mL vs. median 22.2, range 8.4-63.1 U/mL, P=0.001). In the univariate logistic regression analysis, a higher sCD30 (${\geq}30.2U/mL$) was significantly associated with ATL development (odds ratio 7.88 and the 95% confidence intervals 1.35-45.8, P = 0.02). Among cases, sCD30 concentration tended to increase at the time of diagnosis of aggressive-type ATL, but the concentration was stable in those developing the smoldering-type. This suggests that sCD30 may serve as a predictive marker for the onset of aggressive-type ATL in HTLV-1 carriers.

• 미생물과산업
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• 제16권1호
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• pp.2-6
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• 1990

이글에서는 human retrovirus인 성인 T세포 백혈병 바이러스(human T-cell leukemia virus type I : HTLV-I)을 모델로 하여 retrovirus의 구조단백질의 생합성을 조절하는 분자기구에 대해 간단히 소개하고자 한다.

• 약학회지
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• 제31권5호
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• pp.253-265
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• 1987

To develop hybridomas secreting monoclonal antibodies to be used as unlimited sources of reagents indispensable for the diagnosis and treatement of leukemic malignancy, a monoclonal antibody was generated to human pre-T leukemia cells (Jurkat). Hybridomas were produced against Jurkat cell line by fusing spleen cells from hyperimmunized mice with murine plasmacytoma cells (P3$\times$63Ag8. V653). One monoclonal antibody derived from this fusion, designated DMJ-2 was reactive with T-cell lines (Jurkat, Molt-4 and RPMI-8402) and normal peripheral E-rosette forming T cells, but unreactive with B-cell lines (Daudi, Nalm-6) and non-T, non-B cell line (K562). Conclusively DMJ-2 reactive with mature and immature T-lineage lymphoid cells.

• Journal of Microbiology and Biotechnology
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• 제9권3호
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• pp.311-317
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• 1999

Human T-cell leukemia virus Type-I (HTLV-I) is etiologically associated with rare adult T-cell leukemia, a malignant T-cell disorder. cDNAs encoding p24 (gag), gp21(env), and pXII of HTLV-I were amplified by polymerase chain reaction (PCR) using the genomic DNA extracted from HUT102 cell line as a template. The amplified cDNAs were cloned into the Escherichia coli expression vectors and over-expression of the recombinant proteins were achieved by adding IPTG into the culture media in order to induce the promoter. The molecular weights of the recombinant p24, gp21, and pXII, determined by SDS-PAGE, were found to be approximately 28 kDa, 23 kDa, and 15 kDa, respectively. Reactivity of the recombinant proteins with human sera was tested by the immunoblot assay. The gp21 and p24 reacted against the sera obtained from HTLV-I-infected individuals but not against the sera obtained from normal persons. These results suggest that the recombinant proteins expressed in E. coli were recognized by antibodies in sera from HTLV-I infected patients. These recombinant proteins would be applicable for detecting the presence of antibodies against HTLV-I in human blood samples.

• 동의생리병리학회지
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• 제23권6호
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• pp.1314-1319
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• 2009

In previous study, we have shown that continentalic acid (CA) isolated from Aralia continentalis induced the growth inhibition and apoptosis in HepG2 cells. In this study, we examine the effects of CA from A. continentalis on growth inhibition and apoptosis induction in human leukemia HL-60 and mouse fibroblast NIH 3T3 cell lines. The results demonstrated that CA decreased cell growth of leukemia HL-60 cells but not human HaCaT keratinocytes, assessed with the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and LDH (lactate dehydrogenase) assay. Flow cytometric analysis of mouse fibroblast cell lines exposed to CA showed that apoptotic cells increased in a time- and dose-dependent manner. Treatment with CA decreased the number of normal cells and increased the number of early apoptotic and late apoptotic cells in a dose-dependent manner. The induction of apoptosis in mouse cell lines by CA was mediated through the activation of caspase-3, Bak, and Bax and the down-regulation of Bcl-2. Our results suggest that CA efficiently induces apoptosis in human leukemia cells.

• BMB Reports
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• 제45권2호
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• pp.85-90
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• 2012

Our previous study demonstrated that CopA3, a disulfide dimer of the coprisin peptide analogue (LLCIALRKK), has antibacterial activity. In this study, we assessed whether CopA3 caused cellular toxicity in various mammalian cell lines. CopA3 selectively caused a marked decrease in cell viability in Jurkat T, U937, and AML-2 cells (human leukemia cells), but was not cytotoxic to Caki or Hela cells. Fragmentation of DNA, a marker of apoptosis, was also confirmed in the leukemia cell lines, but not in the other cells. CopA3-induced apoptosis in leukemia cells was mediated by apoptosis inducing factor (AIF), indicating induction of a caspase-independent signaling pathway.

• Asian Pacific Journal of Cancer Prevention
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• 제14권4호
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• pp.2447-2451
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• 2013

Objective: To study changes of tumor associated carbohydrate antigen (TACAs) expression and mRNA levels for tumor associated glycosyltransferases, and assess subcellular localizations of N-acetyl galactosyltransferases (GalNAc-Ts) in the K562 leukemia cell line after imatinib treatment. Methods: RT-PCR was performed to analyze the expression of glycosyltransferases which synthesize O-glycan in tumor-associated carbohydrate antigens (TCTAs). The expression of Tn antigen, T antigen and sialyl T antigen on K562 cell membranes was measured by flow cytometry after treatment with different concentrations of imatinib. Co-localization of GalNAc-Ts and ER (endoplasmic reticulum) was determined by confocal laser scanning microcopy. Results: Transcript expression levels of several glycosyltransferases related to TCTAs were decreased after imatinib ($0-0.3{\mu}M$) treatment. Expression of Tn antigen and T antigen was increased while that of sialyl T antigen was decreased. Co-localization of GalNAc-Ts and ER was reduced by $0.2{\mu}M$ of imatinib. Conclusion: Imatinib inhibited the expression of O-glycan related TACAs and several related glycosyltransferases, while decreasing the co-localization of GalNAc-Ts and ER and normalizing O-glycosylation in the K562 human leukemia cell.

• Archives of Pharmacal Research
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• 제18권6호
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• pp.396-401
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• 1995

As a part of trials to develop the antitumor agent from tannins isolated from plants, the antitumor activity of peduculagin, an ellagitannin, isolated from Alnus hirsuta var. microphylla was examined in vitro and in vivo. In vitro, the cytotoxicity was determined by 0.4% typanblue dye exclusion method. peduculagin showed the dose-dependent cytotoxicity against human chronic myelogenous leukemia (K-562), human promyelocytic leukemia (HL-60), mouse lymphoid neoplasm (P388), mouse lymphocytic leukemia (L1210) and mouse sarcoma 180(S180) cell lines. $ED_{50}\; values\; (ED_{50})$ of each cell line were 5.30, 0.92, 2.78, 9.35 and $1.38 \mug/ml$ respectively. The most sensitive cell line was HL-60. In vivo, pedunculagin was administered to ICR mouse with the doses of 50 and $100{\;}{\mu}g/ml$intraperitoneally once at 20 days before S180 inoculation. peduculagin showed the antitumor activity and its T/C ratio (%) was 120.82% in the group of both concentrations.

• 한국균학회지
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• 제27권4호통권91호
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• pp.312-317
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• 1999

우리나라 중요한 식품 원료인 전통 매주로부터 분리한 단독균의 접종 메주의 암세포 저해효과를 검색하기 위하여, 민간유래의 혈액암 세포주에 대한 저해활성을 MTT assay로 분석하였다. 먼저 전통 메주로부터 21종의 단독균을 분리한 후 각각 접종하여 발효된 단독균의 메주시료를 조제한 다음 80% methanol로 추출하였다. 메주 메탄올추출물은 혈액암세포중 HL60에서는 다소 낮은 성장 저해효과를 보였으나, U937과 Jurkat cell에서는 저해효과가 큰 것으로 나타났다. 특히 Mucor속과 Absidia속 Aspergillus속으로 제조된 메주들에서 이들 혈액암세포에 대해 저해효과가 큰 것으로 나타났다. 그러나 모든 메주 메탄올추출물들은 인간의 정상 lymphocyte에서 대해서는 90% 이상의 높은 생존율을 나타내어 정상 세포에 대한 성장 저해 효과가 거의 없음을 보며주었다. 이는 단독균의 메주시료가 가지는 세포독성이 암세포에 대한 특이적인 작용인 것으로 나타났다.

• Parasites, Hosts and Diseases
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• 제31권3호
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• pp.215-222
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• 1993

Toxoplasmngon gondii의 세포내 배양에 적합한 숙주 세포 주를 찾기 위하여 정상 세포 2종류(MDCK-canine kidney cells; Vero-monkey kidney cells) 및 암세포 6종류(A 549, PC 14-human lung cancer cells; SNU 1, SNU 16, MKN 45-human stomach cancer cells; HL-60-human promyelocytic leukemia cells)를 대상으로 하여 각 세포 주의 T.gondii 감염에 대한 감수성을 형태학적 관찰 및 3H-uracil 흡수 시험을 통하여 비교하였다. T.gondii 대한 감수성은 A 549 및 PC 14 세포가 가장 높았고, Vero, HL-60, MDCK 및 SNU 1 세포가 그 다음, SNU 16 및 MKN 45 세포는 가장 감수성이 낮았다. 또한 각 세포 주에 있어서 T.gondii 감염 후 충체증식 정도를 정량화하여 12시간, 36시간 및 60시간에 각각 측정한 바 충체 수를 적게($2{\times}10^5/ml$) 투여했을 때는 A 549, PC 14, Vero, MDCK 세포들에서 감염 60시간까지 충체의 분열 증식이 계속 증가하였고, 충체 수를 많이($50{\times}10^5/ml$) 주입하였을 때는 대부분의 세포들에서 감염 12시간에 최고의 증식을 보이다가 이후 증식이 감소하였다. 이상의 결과로 보아 기son사거 분리 계대 및 충주(strain) 확립을 위해서는 A 549 및 PC 14 세포가 가장 적합할 것으로 판단되며, 충체 주입 수 및 배양 시간별로 충체의 증식 정도가 다름을 알 수 있었다.