• Title/Summary/Keyword: hybrid progenies

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Progeny Analysis of Hybrid Lilies Crossed Between Several Genotypes and Tetraploid Oriental-Asiatic Hybrids (체세포 염색체가 배가된 4배체 Oriental-Asiatic(OA) hybrid 유래 종간잡종 백합의 후대검정)

  • Chung, Mi-Young;Chung, Jae-Dong;Van Tuyl, Jaap M.;Lim, Ki-Byung
    • Korean Journal of Breeding Science
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    • v.42 no.1
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    • pp.100-108
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    • 2010
  • This study was carried out to confirm the chromosome constitution and homoeologous recombination of progenies derived from various cross combination using tetraploid OA interspecific hybrid originated from mitotic chromosome doubling. Based on the chromosome analysis of progenies crossed reciprocally, there were only triploid progenies when crossed with diploid Asiatics as male or female parent. While only tetraploid progenies were produced when crossed tetraploid Asiatics or tetraploid OA hybrid with tetraploid OA hybrid, respectively. However, two types of progenies, that is, diploid and triploid plants, were produced from cross combinations between diploid Oriental hybrid and tetraploid OA hybrid. From the GISH analysis of OA hybrid, it was confirmed that diploid $F_1$ OA hybrid was consisted of 24 chromosomes (12 Oriental and 12 Asiatics) showing authentic OA hybrid. On the other hand, it was notified that triploid plants (3x=36) were consisted of 24 Asiatics lily chromosomes and 12 Oriental lily chromosomes by analysis of backcross progenies derived from either $A{\times}OA$ or $OA{\times}A$ crosses. In cross between tetraploid OA and OA, all the progenies were tetraploid with equal number of chromosomes without any homoeologous recombination, i.e. each 24 chromosomes of Oriental and Asiatics. In 2x-4x ($O{\times}OA$) cross combination, some progenies had 2x=24 chromosomes originated from only Oriental hybrid, and other progenies had 3x=36 chromosomes derived from 24 chromosomes of Oriental hybrid and 12 chromosomes of Asiatic hybrid. Only tetraploid Asiatics chromosomes without any Oriental one were produced in all the progenies from 4x-4x ($AA{\times}OA$) cross combination.

Development of the Brown Planthopper, Nilaparvata lungens Sta$\{aa}$l, Biotypes and Their Hybrid Progenies Fed on Different Cultivars of Rice with Various Resistance Gene and Damage of Different Rice Cultivars (벼멸구 생태형의 교잡종별 발육 및 수도 품종별 피해)

  • 최주수;박영도
    • Journal of Life Science
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    • v.7 no.4
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    • pp.347-354
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    • 1997
  • Some attempts were made to investigate the gentic characteristics of the brown planthopper(BPH), Nilaparvata lugens Sta${\aa}$l, biotypes. In terms of egg and nymphal periods, egg hatcgability, emergence ratio, and population change of BPH and plant damage of rice cultivars by the infestation of BPH biotypes and their hybrid progenies in field conditions. The egg and nymphal periods of the three of BPH biotypes and their hybrid progenies on Dongjinbyeo with no resistance gene were shorter than on rice cultivars with resistance gene. But the periods of biotype-2(♀) X 2(♂) (E) on Samgangbyeo with Bph-1 gene and biotype-3(♀) X 3(♂)(I) on Mirying 63 with bph-2 gene were as short as on Dongjinbyeo. The egg hatchability and emergence ratio of the three Bph biotypes and their hybrid progenies on Dongjinbyeo were higher than on the other rice cultivars, and those of E on Samgangbyeo and I on Miryang 63 were as high as on Dongjinbyeo. In rice paddy field, biotype-1(♀) X 1(♂)(A)$\sim$I infested on Dongjinbyeo, E onSamgangbyeo, and I on Miryang 63 were increased their population densities remarkably in next generation. Dongjinbyeo infested by A$\sim$I, and Myryang 63 infested by I were hopperburned at 60 days after infestation and had a damage rating of 0.9. But Samgangbyeo infested by E was no hopperburn(damage rating: 7.3) though it showed high BPH population at 60 days after infestation.

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Characteristics of Control-Pollinated Progenies of Pinus thunbergii$\times$P. densiflora (해송$\times$소나무 $F_1$ 교잡종의 특성)

  • Lee, Cheul-Ho;Kim, Kyu-Sick
    • Korean Journal of Plant Resources
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    • v.20 no.5
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    • pp.442-445
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    • 2007
  • Seedlings height of hybrids varied depending on mating combinations. Among the progenies from the mating combination, P. thunbergii(CN4)$\times$P. densiflora(KW29) showed the best plant growth. The mean height of this combination was 521 cm at the age of 8 years(yrs), which was 9% higher than rest of the crosses. Seedlings height of progenies using P. thunbergii(JN37) as mother tree showed 28%, 33%, and 29% higher than those of open-pollinated progenies of the mother tree at the ages of 4yrs, 6yrs, and 8yrs, respectively. On the other hand, using P. densiflora (KB5) as father tree showed 45%, 48%, and 32% higher than those of open-pollinated progenies of the father tree. This result may be caused by hybrid vigor.

Selection of Pitch-loblolly Hybrid Pine Plus Tree Progenies for Cold-hardiness (리기테다 수형목(秀型木) 차대(次代)들의 내한성(耐寒性)에 대(對)한 선발(選拔))

  • Park, Mun Han;Sohn, Sung In;Jhun, Gae Sang
    • Journal of Korean Society of Forest Science
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    • v.75 no.1
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    • pp.46-50
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    • 1986
  • One-year-old seedlings of open-pollinated progenies of pitch-loblolly hybrid pine plus trees were selected for cold-hardiness during cold winter of 1983-1984. Survived trees were planted in three locations. Two years later, the ratio of cold-damaged trees and 3rd year heights were recorded. The ratio of the, cold-damage trees among screened progenies were 5.6% in Kyunggi and 22.6% in Chungnam, while 9.3% and 43.3%, respectively, in unscreened control plot. Severely damaged families at one-year-old seedling stage also suffered heavy cold damage after two years. Not much differences were observed between families in 3rd year height.

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The Characteristics of Progenies derived from Lilium lancifolium and Asiatic hybrid "Dreamland" (참나리와 Asiatic hybrid "Dreamland"의 교잡 후대 특성)

  • Park, In-Sook;Suh, Dong-Hee;Hwang, Yoon-Jung;Park, Song-Kyung;Kang, Si-Yong;Rhee, Hye-Kyung;Lim, Ki-Byung
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.451-455
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    • 2009
  • Lilium lancifolium, which is one of the Korean native lilies, possesses several useful genetic characteristics such as growth vigor, bulbil formation and resistance to Fusarium. Hybridization was performed to insert useful traits of Asiatic hybrid "Dreamland" as the male parent with upward direction and dark brown spotless on the petal into L. lancifolium as the female parent. The rate of pollen germination of L. lancifolium and "Dreamland" were 30% and 60%, respectively. The pollen germination ratio of $F_1$ progenies at flowering date was 25-57%, which is between the parents'pollen germination ratio. The individual flowering time was between 113 days and 131 days after planting. Of 99% of all plants, the leaf shape index was more than five, which means that the leaf shape of $F_1$ plants is more influenced by female than male parent. The flower color was separated into four types. 51.5% of the progenies showed orange and 44.2% showed light orange, respectively. The colors seemed to be highly influenced by female parents. Mixed colors of parents, orange yellow and light yellow orange, were 3.1%, 1.2%, respectively. Flowering directions were segregated by three different ways, upside, side, and downside. Side direction, which is medium type between female and male parent, was 73%. All progenies showed lots of spot on petals except one offspring. The percentage of bulbil formation on leaf axil was 49.4%. Among those, the maximum number of bulbil formation was 25. The mean number of bulbil formation per plant was five.

Varietal Resistance of Rice to the BPH Biotypes 1, 2, 3 and their Hybrid Progenies, Nilaparvata lugens $St{\aa}l$ (I) (벼멸구 생태형(生態型)의 교잡종(交雜種)에 대(對)한 수도품종(水稻品種)의 저항성(抵抗性)에 관(關)하여(I))

  • Kim, Jeong-Wha;Youn, Sang-Won
    • Korean journal of applied entomology
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    • v.26 no.2 s.71
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    • pp.63-69
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    • 1987
  • This study was performed to evaluate the resistance of three different rice cultivars to the BPH biotypes 1, 2, 3 and their hybrid progenies, Nilaparvata lugens STAL. The rice cultivars tested were Chucheongbyeo, Cheongcheongbyeo and Milyang 63. The following characteristics of hybrid progenies were compared with these of their parents: Seedling response, Host preference, feeding amount, Esterase polymorphism of the brown rice. According to the responses of rice seedlings to the three BPH biotypes and their crosses progenies nymph, Chucheongbyeo was shown highly susceptible to their nymphs. The Cheongcheongbyeo was susceptible to the biotypes 2 and the Milyang 63 was susceptible to the biotype 3. The Cheongchengbyeo and the Milyang 63 were moderate to the biotypes 2(female)${\times}$3(male) and 3(female)${\times}$2(male). The preference in feeding and oviposition of three BPH biotypes and their hybrid progenies to Chucheongbyeo were shown higher than to another cultivars. The two preference were not only shown highly in Cheongcheongbyeo by the biotypes 2,2(female)${\times}$3(male) and 3(female)${\times}$2(male) but also in Milyang 63 by the biotypes 3,2(female)${\times}$3(male) and 3(femlae)${\times}$2(male). The feeding amount of female adult was much on three BPH biotypes and their hybrid progenies in Chucheongbyeo and on the biotypes 2 and 3(female)${\times}$2(male) in Cheongcheongbyeo, On the biotypes 3 and 3(female)${\times}$2(male) in Milyang 63 were as same as the above. The esterase bands of Est ${\alpha}-1,\;{\beta}-4,\;{\beta}-5\;and\;{\beta}-I$ were detected on brown rice of all the tested cultivars. The bands of Est ${\beta}-2\;and\;{\alpha}-I$ were detected on Cheongcheongbyeo (Bph 1 gene for resistance) and Milyang 63 (bph 2 gene for resistance). The Est ${\beta}-3\;and\;{\beta}-1$ of esterase bands were detected on Chucheongbyeo.

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Fertility Restoration of F1 OA Interspecific Hybrid by Spontaneous Meiotic Polyploidization and Analysis of Their Progenies (자발적 생식세포 염색체 배수화 기작에 의한 Oriental-Asiatic hybrid(OA) F1 종간잡종의 임성회복과 후대검정)

  • Chung, Mi-Young;Chung, Jae-Dong;Van Tuyl, Jaap M.;Lim, Ki-Byung
    • Korean Journal of Breeding Science
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    • v.41 no.3
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    • pp.213-219
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    • 2009
  • This study was carried out to establish breeding system for interspecific hybridization of Lilium using spontaneous meiotic polyploidization of $F_1$ OA hybrid and its progenies. Pollen viability and germination of $F_1$ OA interspecific hybrid showed variation by collection time, indicating that pollen grain in June, which was natural blooming season, had better viability and germination ability than in October. 2n-gametes from spontaneous meiotic polyploidization of OA hybrid were crossed with either diploid Asiatic or Oriental hybrid, and subsequent progenies crossed with Asiatic hybrid as female and OA hybrid as male showed the highest efficiency, where 2x-2x A$\times$OA produced 0.64 plants per cross combination, while 2x-2x, O$\times$OA and 2x-2x, OA$\times$A crosses produced 0.42 and 0.4 plant per ovary, and especially no progeny was obtained in 2x-2x, OA$\times$O cross combination. For the subsequent progeny production, a cross between diploid Asiatic hybrid as female and diploid OA hybrid as male was the best ploidy combination. Ploidy level of subsequent progeny derived from backcross with 2n-gametes of OA hybrid revealed that triploids from 2x-2x OA$\times$A, tetraploids from both 4x-2x, AA$\times$OA and OO$\times$OA, diploid from 2x-2x, O$\times$OA, and diploids or triploids from 2x-2x, A$\times$OA crosses were produced respectively.

Characteristics of Pinus thunbergii×P. massoniana F1 Hybrid Seedlings (Pinus thunbergii×P. massoniana F1 교잡종(交雜種) 유묘(幼苗)의 특성(特性))

  • Park, Mun Han;Jhun, Gae Sang;Youn, Yang
    • Journal of Korean Society of Forest Science
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    • v.69 no.1
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    • pp.51-55
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    • 1985
  • Pinus thunbergii ${\times}$ P. massoniana $F_1$ hybrids were made by pollinating 15 years old Japanese black pine (P. thunbergii) with Masson pine (P. massoniana) pollen, air-shipped from Taiwan. The crossability between two species were observed. Seedling height, morphological characteristics, and the composition of phenolic compounds of these hybrids and the open-pollinated progenies of P. thunbergii were investigated. The results of the observation were as follows; 1) Number of the hybrid seeds per cone was 2.3, while open-pollinated Japanese black pine produced 26 seeds per cone. 2) All hybrid seedlings outgrew P. thunbergii, averaging 151% of mean height of Japanese black pine seedlings. The growth of hybrids was much variable, ranging from 120% to 208% of mean height of the open-pollinated progenies of P. thunbergii. 3) With regard to needle characteristics, the hybrid needles were longer and softer than those of P. thunbergii. The color of hybrid needles showed yellowish green. The positions of resin ducts in P. thunbergii observed medial, while those of hybrids showed medial and external. 4) No large difference in phenolic compound composition between hybrid and P. thunbergii was found, but phenolic compound of unknown 4 and 6 were observed only in hybrid. Further intensive work is recommended in this area.

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Silvicultural and Genetic Studies on Isozyme Patterns in Forest Trees - Inheritance of Leucine Aminopeptidase and Peroxidase Isozymes in ×Pinus taeda·rigida and P. densiflora - (유용임목(有用林木)의 동위효소(同位酵素)에 관(關)한 조림(造林) 및 유전학적(遺傳學的) 연구(硏究) - ×Pinus taeda·rigida와 P. densiflora에 있어서 Leucine Aminopeptidase와 Peroxidase 동위효소(同位酵素)의 유전(遺傳) -)

  • Park, Young Goo;Son, Won Ha
    • Journal of Korean Society of Forest Science
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    • v.43 no.1
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    • pp.39-50
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    • 1979
  • Twelve Pinus taeda were used as mother trees and one P. rigida plus tree was used as pollen tree for 12 cross combinations. Nine P. densiflora plus trees(4 mother and 7 pollen trees) were used as parents for 11 cross combinations. Those parents and 10 progenies were analyzed for LAP of ${\times}$P. taeda rigida hybrid and P. densiflora and for peroxidase of ${\times}$P. taeda rigida. The analysis, based on the banding patterns, indicate three alleles for LAP-A locus(A1, A2, A3) and two alleles for LAP-B locus (B1, B2) in ${\times}$P. taeda rigida hybrids. Chi-square test on the segregation for progenies did not show significant differences. The results indicated good agreement with monohybrid Mendelian inheritance. Independence test for occurrence frequency of 2 alleles(LAP-A3, LAP-B2) illustrated that there is neither linkage nor repulsion relationship between LAP-A3 and LAP-B2 alleles. Three band at LAP-A locus were always exhibited from all parents and their progenies of P. densiflora. However, the occurrence of two bands at LAP-B locus was variable, one bands assumed as homozygous alleles(B2/B2) and two bands as heterozygous alleles(B1/B2). The segregation ratio for progenies of P. densiflora suggested that LAP-B locus may be controlled by two alleles(B1 and B2). Three Peroxidase loci(Px-A, Px-B, Px-C) assumed to be controlled by allozyme in ${\times}$P. taeda rigida hybrid. The Px-B and Px-C loci could not find out the variations from banding patterns of parents and their progenies, while the Px-A locus showed the variations of occurrence frequency by two bands. The segregation ratio for A1/A2 at LAP-A locus suggest that the peroxidase allozymes of ${\times}$P. taeda rigida hybrid appeare to be monomeric products; that is, Px-A locus may be controlled by two alleles (A1 and A2).

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A New Hybrid, Dark Pink Spotted Type Phalaenopsis 'Pink Marble'

  • Kim, Mi-Seon;Lee, Young-Ran;Rhee, Hye-Kyung;Park, Sang-Kun;Shin, Hak-Ki;Jung, Hyang-Young;Lim, Jin-Hee
    • Horticultural Science & Technology
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    • v.29 no.5
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    • pp.503-506
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    • 2011
  • A new hybrid, Phalaenopsis 'Pink Marble' was made by the National Institute of Horticultural and Herbal Science, Rural Development Administration, in 2005. This hybrid was selected from self-crossed progenies of P. '21-1' (collected number) in 1999. In 2001, one line was selected based on the aspects such as flower color, leaf shape, flower stalk, and vigorous growth. Trials were conducted from 2003 to 2005 for evaluation and selection of this cultivar. 'Pink Marble' had a medium flowering habit and a dark pink spot (RHS, RPN74B) on white petal and sepal when fully opened. The number of flowers on each peduncle was 7.5, and flower diameter was 52.3 cm. The general impression of petals and sepals is a plate shape. The thick sepal could extend the long flowering time. The average length of leaf and peduncle were 16.5 cm and 6.8 cm, respectively. It had a half-erect leaf form, and was a fast-growing cultivar. This hybrid is relatively easy to clone.