• Title, Summary, Keyword: hydrogen peroxide

Search Result 2,042, Processing Time 0.037 seconds

Effect of Scutellariae Radix Water Extract on Hydrogen Peroxide Production in LPS-induced RAW 264.7 Mouse Macrophages (황금(黃芩) 물추출물이 LPS로 유발된 마우스 대식세포의 hydrogen peroxide 생성에 미치는 영향)

  • Park, Wan-Su
    • Korean Journal of Acupuncture
    • /
    • v.28 no.3
    • /
    • pp.33-41
    • /
    • 2011
  • Objectives : The purpose of this study is to investigate effects of Scutellariae Radix water extract on hydrogen peroxide production in LPS-induced RAW 264.7 mouse macrophages. Methods : Scutellariae Radix produced from South Korea (SK) and Scutellariae Radix produced from China (SC) were extracted by hot water. Effects of SK and SC on hydrogen peroxide production in LPS-induced RAW 264.7 were measured by dihydrorhodamine 123 assay after 20, 24, 28, 44, 48, and 52 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL. Results : SK significantly increased hydrogen peroxide production in LPS-induced RAW 264.7 cells for 20, 24, 28, 44, 48, and 52 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL (P < 0.05). But SC did not represent any significant effect on hydrogen peroxide production in LPS-induced RAW 264.7 cells. Conclusions : These results suggest that Scutellariae Radix, especially produced from South Korea, has the immune-enhancing property related with its increasement of bacteriocidal hydrogen peroxide production in LPS-induced macrophages.

Ultraviolet Microscopic Study on Lignin Distribution in the Fiber Cell Wall of BCTMP

  • Yoon, Seung-Lak;Yasuo Kojina
    • Journal of Korea Technical Association of The Pulp and Paper Industry
    • /
    • v.36 no.1
    • /
    • pp.61-66
    • /
    • 2004
  • Bleached chemithermomechanical pulp (BCTMP) was produced from CTMP of Betula maximowicziana Regel by two staged ozone-hydrogen peroxide bleaching in order to improve the optical properties of high yield pulp. This pulp was used for the evaluation of optical properties improvement, chemical characteristics of lignin in fiber and the relationship between lignin and optical properties in fiber cell wall. Hydrogen peroxide treatment improved the brightness, but the post color number (PC No.). There was little improvement on optical properties by ozone treatment, but this could be improved more by using two staged ozone-hydrogen peroxide bleaching. The hydrogen peroxide treatment did not make any change on chemical characteristics of lignin in cell wall, but by ozone treatment, it was found that the non-aromatic conjugated structure was existed in the surface of cell wall, but this could be removed by hydrogen peroxide treatment in two staged ozone-hydrogen peroxide treatment. Therefore, the optical properties was significantly improved, due to the removal of non-aromatic conjugated structure.

Effects of Sintongchukeo-tang on the Cultured Spinal Sensory Neurons Injured by Hydrogen Peroxide (신통축어탕(身痛逐瘀湯)이 Hydrogen Peroxide에 의해 손상(損傷)된 배양(培養) 척수감각신경세포(脊髓感覺神經細胞)에 미치는 영향(影響))

  • Lee, Kye-Seung;Na, Young-Hoon;Cha, Yong-Suk;Heo, Yun;Kim, Do-Hwan;Han, Sang-Hyok;Park, Byong-Min;Lee, In;Moon, Byung-Soon
    • The Journal of Internal Korean Medicine
    • /
    • v.22 no.4
    • /
    • pp.557-565
    • /
    • 2001
  • Objectives : This study was carried out to examine toxic effect of Sintongchukeo-tang on cultured mouse spinal sensory neurons inhibited by neurotoxicity induced by hydrogen peroxide. Methods : MTT assay, NR assay, LDH and neurofilament assay were performed after spinal sensory neurons were preincubater with various concentrations of Sintongchukeo-tang water extract before treatment of cells with hydrogen peroxide. Results : Hydrogen peroxide induced ceil degeneration such as the decrease of cell viability was measured by MTT and NR assay in the cultured mouse spinal sensory neurons. Sintongchukeo-tang water extract was effective in the decrease of LDH activities of neurons produced by hydrogen peroxide. Sintongchukeo-tang water extract was effective in the increase of amount of neurofilaments damaged by hydrogen peroxide. Conclusions : From the above results, it is suggested that hydrogen peroxide induces the inhibition of cell viability in cultured mouse spinal sensory neurons and Sintongchukeo-tang water extract was effective in cultured neurons damaged by hydrogen peroxide.

  • PDF

The Influence of Hydrogen Peroxide Treatment on Water Stress, Photosynthesis and Thermotolerance of Cucumber(Cucumis sativus) in Greenhouse Cultivation during Summer (Hydrogen Peroxide 처리가 여름철 시설오이의 수분 스트레스, 광합성, 내서성에 미치는 영향)

  • Woo Young-Hoe;Kim Hyung-Jun;Kim Tae-Young;Kim Ki-Deog;Huh Yun-Chan;Chun Hee;Cho Ill-Hwan;Nam Yooun-Il;Ko Kwan-Dal;Lee Kwan-Ho;Hong Kue-Hyon
    • Journal of Bio-Environment Control
    • /
    • v.15 no.1
    • /
    • pp.47-52
    • /
    • 2006
  • This studies were carried out in summer season to increase high temperature tolerance using hydrogen peroxide treatments on cucumber in greenhouse. The water stress of cucumber in greenhouse by the hydrogen peroxide treatments showed as control>250 mM>500 mM treatments in order. The photosynthesis rate of cucumber at $30^{\circ}C$ did not show difference with each hydrogen peroxide treatment in temperature controlled greenhouse. However, the photosynthesis rate of cucumber in the control and hydrogen peroxide treatments at $40^{\circ}C$ was significantly different. The photosynthesis rate of cucumber in combined treatment with 1,000 $mg{\cdot}L^{-1}\;CO_2$ supply and hydrogen peroxide was also higher than control, however, there was no different of photosynthesis in 250 mM and 500 mM treatment. The value of $F_v/F_m$ and $F_m/F_o$ of chlorophyll fluorescent in 500 mM hydrogen peroxide treatment at $40^{\circ}C$ was highest. Also the activity of POD, the antioxidant enzyme, was higher with high hydrogen peroxide concentration than the other treatments. The high temperature limits for growth were $43^{\circ}C$ in the control, $44^{\circ}C$ in the 250 mM and $46^{\circ}C$ in the 500 mM according to analyze chlorophyll fluorescent $F_o$. The high temperature tolerance in cucumber increased approximately $3^{\circ}C$ by the hydrogen peroxide treatments under this experiment conditions.

Decolorization of Melanin by Lignin Peroxidase from Phanerochaete chrysosporium

  • Woo, Sung-Hwan;Cho, Jeung-Suk;Lee, Baek-Seok;Kim, Eun-Ki
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • v.9 no.4
    • /
    • pp.256-260
    • /
    • 2004
  • Melanin was decolorized by lignin peroxidase from Phanerochaete chrysosporium. This decolorization reaction showed a Michaelis-Mentens type relationship between the decolorization rate and concentration of two substrates: melanin and hydrogen peroxide. Kinetic constants of the decolorization reaction were 0.1 OD$\sub$475//min ($V_{max}$) and 99.7 mg/L ($K_{m}$) for melanin and 0.08 OD$\sub$475//min ($V_{max}$) and 504.9 ${\mu}$M ($K_{m}$) for hydrogen peroxide, respectively. Depletion of hydrogen peroxide interrupted the decolorization reaction, indicating the essential requirement of hydrogen peroxide. Pulsewise feeding of hydrogen peroxide continued the decolorizing reaction catalyzed by lignin peroxidase. These results indicate that enzymatic decolorization of melanin has applications in the development of new cosmetic whitening agents.

Effect of Artemisiae Argi Folium Fermented with Lactobacillus Pentosus on Hydrogen Peroxide Production of Human Hepatocyte Treated with Toxicants (Gallic acid 등으로 유발된 인간 간 조직세포 내 hydrogen peroxide 생성억제에 대한 유산균발효애엽 추출물의 영향)

  • Park, Wan-Su;Kim, Do-Hoon
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.23 no.6
    • /
    • pp.1379-1384
    • /
    • 2009
  • The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium Fermented with Lactobacillus pentosus (AFL) on hydrogen peroxide production within human hepatocyte HepG2 cells treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. AFL (0~400 ug/mL) was treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. AFL showed the restoration of the intracellular productions of hydrogen peroxide which were reduced by gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde in HepG2 Cells. AFL could be supposed to have the hepatoprotective effect related with hepatocytologic signaling activity against gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde.

Effect of Artemisiae Argi Folium Fermented with Lactobacillus Pentosus on Hydrogen Peroxide Production of Macrophage Treated with Toxicants (Gallic acid 등으로 유발된 대식세포 내 hydrogen peroxide 생성억제에 대한 유산균발효애엽 추출물의 영향)

  • Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.23 no.2
    • /
    • pp.438-442
    • /
    • 2009
  • The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium Fermented with Lactobacillus pentosus (AFL) on hydrogen peroxide production within mouse macrophage Raw 264.7 Cells treated with gallic acid, EtOH, Nicotine, Acetaminophen, and Acetaldehyde. AFL (0${\sim}$400 ug/mL) was treated with gallic acid, EtOH, Nicotine, Acetaminophen, and Acetaldehyde. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. AFL showed the restoration of the intracellular productions of hydrogen peroxide which were reduced by gallic acid, EtOH, Nicotine, Acetaminophen in Raw 264.7 Cells. AFL could be supposed to have the immunological activity related with macrophage's oxidative burst.

Effect of Water Extract from Artemisiae Argi Folium on Hydrogen Peroxide Production within Mouse Macrophage Raw 264.7 Cells Treated with Gallic acid, EtOH, LPS, and Acetaminophen (Gallic acid, EtOH, LPS, Acetaminophen으로 유발된 마우스 대식세포 내 hydrogen peroxide 생성억제에 대한 애엽 물추출물의 영향 연구)

  • Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.22 no.6
    • /
    • pp.1495-1499
    • /
    • 2008
  • The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium (WAAF) on hydrogen peroxide production within mouse macrophage Raw 264.7 Cells treated with gallic acid, EtOH, LPS, and acetaminophen. WAAF (0${\sim}$400 ug/mL) was treated with gallic acid, EtOH, LPS, acetaminophen. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. WAAF showed the restoration of the intracellular productions of hydrogen peroxide which were reduced by gallic acid, EtOH, LPS, and acetaminophen in Raw 264.7 Cells. WAAF could be supposed to have the immunological activity related with macrophage's oxidative burst.

Effect of Sacchromyces cerevisiae-Fermented Artemisiae Argi Folium on Hydrogen Peroxide Production of Macrophage Treated with Toxicants (EtOH 등으로 유발된 대식세포 내 hydrogen peroxide 생성억제에 대한 효모균발효애엽 추출물의 영향)

  • Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.23 no.3
    • /
    • pp.608-612
    • /
    • 2009
  • The effect of Sacchromyces cerevisiae-Fermented Artemisiae Argi Folium Water extract (AFS) on hydrogen peroxide production within mouse macrophage Raw 264.7 Cells treated with EtOH, gallic acid, Nicotine, Acetaminophen, and Acetaldehyde was investigated through this study. AFS (0-400 ug/mL) was simultaneously treated with EtOH, gallic acid, Nicotine, Acetaminophen, and Acetaldehyde. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. AFS restorated the intracellular productions of hydrogen peroxide reduced by EtOH, gallic acid, Nicotine, Acetaminophen within Raw 264.7 Cells. AFS could be supposed to have the immunological activity concerned with macrophage's oxidative burst.

Effect of Artemisiae Argi Folium Fermented with Sacchromyces Cerevisiae on Hydrogen Peroxide Production of Human Hepatocyte Treated with Toxicants (Nicotine 등으로 유발된 인간 간조직세포 내 hydrogen peroxide 생성억제에 대한 효모균발효애엽 추출물의 영향)

  • Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.24 no.1
    • /
    • pp.96-101
    • /
    • 2010
  • The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium Fermented with Sacchromyces cerevisiae (AFS) on hydrogen peroxide production within human hepatocyte HepG2 cells treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. AFS (0~400 ug/mL) was treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. AFS showed the restoration of the intracellular productions of hydrogen peroxide which were reduced by gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde in HepG2 Cells. AFS could be supposed to have the hepatoprotective effect related with hepatocytologic signaling activity against gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde.