• Title/Summary/Keyword: immunostimulation

Search Result 62, Processing Time 0.03 seconds

Studies on the Anticomplementary Activity of Korean Higher Fungi (한국산 고등균류의 항보체활성 효과에 관한 연구)

  • Jeong, Hoon;Lee, June-Woo;Lee, Kweon-Haeng
    • The Korean Journal of Mycology
    • /
    • v.18 no.3
    • /
    • pp.145-148
    • /
    • 1990
  • Anticomplementary activity of 61 strains of Korean higher fungi was screened for immunostimulation. Extracts from 11 of 61 strains including 5 of Ganoderma lucidum, 3 of Lentinus edodes, 2 of Cordyceps sp. and 1 of Agaricus campestris, showed higher anticomplementary activity than krestin which was immunopotent extract from Japanese Coriolus versicolor. The most potent anticomplementary activity was found with extract from Lentinus edodes IY105 whose complement consumption was 31.7%.

  • PDF

The effect of polysaccharide isolated from the root of Acanthopanax koreanum on B cell activation

  • Han, S-B;Ahn, H-J;Yoon, Y-D;Kim, Y-H;Lee, J-J;Moon, J-S;Lee, C-W;Lee, K-H;Park, S-K
    • Proceedings of the Korean Society of Toxicology Conference
    • /
    • 2002.11b
    • /
    • pp.183-183
    • /
    • 2002
  • Many polysaccharides isolated from plants have been shown to enhance various immune responses in vivo and in vitro. Here we demonstrate that polysaccharide isolated from the root of Acanthopanax koreanum (AK) has a unique mode of immunostimulation with regard to its cell-type specificity.(omitted)

  • PDF

Immunoactivities of PVMP, a Protein-polysaccharide Fraction Isolated from Mycelial Culture of Psathyrella velutina (큰눈물버섯(Psathyrella velutina) 균사배양물로부터 분리한 단백다당체 PVMP의 면역활성)

  • 정경수;이지선
    • YAKHAK HOEJI
    • /
    • v.48 no.5
    • /
    • pp.261-265
    • /
    • 2004
  • In the previous report, we described the marked antitumor and immunomodulatory activities of PVp, a protein-polysaccharide fraction of a Korean wild mushroom Psathyrella velutina. In this study, a protein-polysaccharide fraction, PVMP, was prepared from the shake-cultured mycelia of the same mushroom and its immunoactivities as well as chemical compositions were investigated. At 200 $\mu\textrm{g}$/ml, PVMP weakly stimulated the BALB/c mouse splenic lymphocytes to form lymphoblasts and upregulated the expression of CD25 molecules, but failed to stimulate peritoneal macrophages. In chemical analysis these two protein-polysaccharide fractions were found to be quite different in that the carbohydrate contents of PVMP and PVP, respectively, was 85.3% and 41.2%. These results reveals that PVMP, unlike PVP, is a moderate immunostimulator on the immune system.

A Flow Cytometrical Analysis of the Antitumor and Immunostimulatory Effects of LCT-CT, a Cold-water Extract Prepared from Rice Grasshopper Oxya japonica japonica Thunberg (벼메뚜기(Oxya japonica japonica Thunberg) 물 추출물 LCT-CT의 항암면역 활성에 관한 유세포 분석학적 연구)

  • Chung, Kyeong-Soo;Kim, Bit Na
    • YAKHAK HOEJI
    • /
    • v.58 no.3
    • /
    • pp.151-157
    • /
    • 2014
  • Water extracts of rice grasshopper (Oxya japonica japonica Thurnberg) were prepared and their antitumor and immunostimulatory activities were investigated using a flow cytometer. When LCT-CT was ip injected into ICR mice at the dose of 33.3 mg/kg before and after the implantation of $4{\times}10^5$ cells/mouse of sarcoma 180 tumor cells, it inhibited the growth of the tumor cells by 96.6%, showed lymphoblstogenic activities on the splenic lymphocytes and increased the expression of CD25 molecules on the splenic T lymphocytes. When co-cultured with the splenic lymphocytes of a BALB/c mouse, LCT-CT showed strong immunostimulatory activities at the concentration of $25{\sim}100{\mu}g/ml$ by significantly increasing lymphoblasts ratio and CD25 expression.

An antitumor component of laetiporus sulphureus and its immunostimulating activity

  • Kang, Chang-Yuil;Lee, Chong-Ock;Chung, Kyeong-Soo;Choi, Eung-Chil;Kim, Byong-Kak
    • Archives of Pharmacal Research
    • /
    • v.5 no.2
    • /
    • pp.39-43
    • /
    • 1982
  • A protein-polysaccharide fraction was prepared from the carpophores of Laetiporus sulphureus. This fraction suppressed growth of sarcoma 180 in A-strain mice when administered i. p. To investigate the mechanism of antitumor action of this fraction, plaque assay was conducted by administrating i. p. to the mise at a dose level of 50mg/kg for five days. Ten days later, the mice were immunized with 1 * 10$^{7}$ sheep red blooc cells. The number of hemolytic plaque forming cells was significantly greater than that of the control mice. Three monosaccharides and fifteen amino acids were identified in the protein-polysaccharide fraction.

  • PDF

Immunomodulatory activity of a polysaccharide isolated from cell culture of Acantopanax senticosus

  • Ahn, Hyo-Jung;Han, Sang-Bae;Yoon, Yeo-Dae;Lee, Haeng-Soon;Moon, Jae-Sun;Lee, Chang-Woo;Lee, Ki-Hoon;Park, Song-Kyu;Kim, Hyung-Chin;Kim, Hwan-Mook
    • Proceedings of the PSK Conference
    • /
    • 2002.10a
    • /
    • pp.259.1-259.1
    • /
    • 2002
  • Many polysaccharides isolated from plants are considered to be biological response modifiers and have been shown to enhance various immune responses in vivo and in vitro. Here we demonstrate that a polysaccharide isolated from cell culture of Acanthopanax senticosus (AS) has a unique mode of immunostimulation with regard to its cell-type specificity. AS was found to markedly increase polyclonal IgM antibody production and the proliferation of B cells. and to activate iNOS transcription and NO production in macrophages. (omitted)

  • PDF

Selective B cell activation by polysaccharide isolated from the root of Acanthopanax koreanum

  • Han, Sang-Bae;Ahn, Hyo-Jung;Yoon, Yeo-Dae;Kim, Young-Ho;Lee, Jung-Joon;Moon, Jae-Sun;Lee, Chang-Woo;Lee, Ki-Hoon;Park, Song-Kyu;Kim, Hyung-Chin;Kim, Hwan-Mook
    • Proceedings of the PSK Conference
    • /
    • 2002.10a
    • /
    • pp.257.1-257.1
    • /
    • 2002
  • Many polysaccharides isolated from plants have been shown to enhance various immune responses in vivo and in vitro. Here we demonstrate that polysaccharide isolated from the root of Acanthopanax koreanum (AK) has a unique mode of immunostimulation with regard to its cell-type specificity. AK was found to markedly increase polyclonal 1gM antibody production and the proliferation of B cells. (omitted)

  • PDF

Effects of an Extract from the Roots of Platycodon Grandiflorum on the Levels of p53 and pRB in NCI-H460 Human Lung Carcinoma Cells (길경 수용액 추출물에 의한 NCI-H460 인체 폐암세포의 p53 및 pRB의 발현에 미치는 영향)

  • Park, Bong-Kyu;Gam, Chul-Woo;Heo, Tae-Yool;Park, Dong-Il
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.20 no.6
    • /
    • pp.1530-1537
    • /
    • 2006
  • Platycodi Radix, the root of Platycodon grandiflorum A. DC (Campanulaceae), commonly known as Doraji in Korea (Chinese name, 'Jiegeng', and Japanese name, 'Kikyo') has been used as an expectorant in traditional Oriental medicine. Extracts from the roots of P. grandiflorum have been reported to have wide ranging health benefits. In Korea, Platycodi Radix is also used as a food and employed as a folk remedy for adult diseases, such as bronchitis, asthma and pulmonary tuberculosis, hyperlipidemia, diabetes, and inflammatory diseases, and as a sedative. Several studies on its chemical and immunopharmacological effects including immunostimulation and antitumor activity have been performed. However, the relevant molecular mechanisms are poorly understood. Platycodi Radix, the root of Platycodon grandiflorum A. DC (Campanulaceae), commonly known as Doraji in Korea (Chinese name, 'Jiegeng', and Japanese name, 'Kikyo') has been used as an expectorant in traditional Oriental medicine. Extracts from the roots of P. grandiflorum have been reported to have wide ranging health bensfits. In Korea, Platycodi Radix is also used as a food and employed as a folk remedy for adult diseases, such as bronchitis, asthma and pulmonary tuberculosis, hyperlipidemia, diabetes, and inflammatory diseases, and as a sedative. Several studies on its chemical and immunopharmacological effects including immunostimulation and antitumor activity have been performed. However, the relevant molecular mechanisms are poorly understood. In the present study, we investigated the effects of an aqueous extract from the roots of P. grandiflorum AEPG) on the cell growth of human lung adenocarcinoma NCI-H460 cells in order to understand its anti-proliferative mechanism. AEPG treatment down-regulated the cyclin D1 expression in both transcriptional and translational levels without alteration of cyclin E. In AEPG-treated cells, the levels of cyclin-dependent kinase (C아) 6 mRNA and protein were significantly inhibited, but the levels of Cdk2 and Cdk4 were slightly inhibited by treatment of AEPG. AEPG treatment induced a marked accumulation of Cdk inhibitors, p16 and p27. However, AEPG treatment did not affect not only retinoblastoma protein (pRB) but also tumor suppressor p53 protein expression. The present results indicated that AEPG-induced inhibition of lung cancer cell proliferation is associated with the blockage of G1 phase progression through induction of Cdk inhibitors such as p16 and p27, and inhibition of cyclin D1 and Cdk6. AEPG exposure, as offered by this study, provides cluse for the mechanism of AEPG action. Taken together, these findings suggest that P. grandiflorum has strong potential for development as an agent for prevention and treatiment against human lung cancer.

Bordetella bronchiseptica is a potent and safe adjuvant that enhances the antigen-presenting capability of dendritic cells

  • Lee, You-Jeong;Han, Yong;Joo, Hong-Gu
    • The Korean Journal of Physiology and Pharmacology
    • /
    • v.24 no.1
    • /
    • pp.47-52
    • /
    • 2020
  • We previously demonstrated that Bordetella bronchiseptica (B. bronchiseptica) antigen (Ag) enhances the Mycoplasma hyopneumoniae Ag-specific immune response. The focus of this study was whether acellular bacterin of B. bronchiseptica could be used as an adjuvant to increase antigen-presenting capability of dendritic cells (DCs) by increasing the level of activation. The metabolic activity of DCs was increased by B. bronchiseptica, similar to lipopolysaccharide (LPS). Flow cytometry analysis revealed that B. bronchiseptica increases the expression of major histocompatibility complex class-2, cluster of differentiation (CD)40, CD54, and CD86 which are closely related to DC-mediated immune responses. B. bronchiseptica enhanced the production of cytokines related to adaptive immune responses. Furthermore, the survival rate of B. bronchiseptica-injected groups was 100% at 15 and 20 mg/kg doses, whereas that of LPS-injected groups was only 20%, 0% at 15 and 20 mg/kg doses respectively, and so B. bronchiseptica is likely to be safer than LPS. Taken together, these results indicate that B. bronchiseptica can be used as an adjuvant to enhance the antigen-presenting capability of DCs. B. bronchiseptica is a candidate for producing vaccines, especially in case of DC-mediating efficacy and safety demands. This study provides researchers and clinicians with valuable information regarding the usage of B. bronchiseptica as a safe bacteria-derived immunostimulating agent for developing efficient vaccines.

Immunoactivity of Ginsenosides Re and Rg1 that Enhances Resistance of Mice Against Experimental Disseminated Candidiasis

  • Han, Yong-Moon;Jin, Byung-Suk;Ko, Sung-Kwon;Lee, Jue-Hee
    • Natural Product Sciences
    • /
    • v.10 no.3
    • /
    • pp.134-139
    • /
    • 2004
  • In this study, an immunoactivity of panaxtriol ginsenosides Re and Rg1 against infection due to Candida albicans was investigated. The ginsenosides were extracted from Red Ginseng with 85% ethanol and heat-treatment and were analyzed by HPLC on water-acetonitrile as a mobile phase. The HPLC analysis revealed that the extract contained ginsenosides Re and Rg1, which were eluted as a combined peak. By agar diffusion susceptibility, the mixture of Re and Rg1 had no growth-inhibitory activity on C. albicans yeast cells. However, in animal tests BALB/c mice given the mixture of Re and Rg1 intraperitoneally (Lp.) before intravenous (Lv.) infection with live C. albicans yeast cells had longer mean survival times (MST) than MST of control mice groups that received only buffer solution instead of Re and Rg1. In experiments 60% of the ginsenosides-treated mice survived the entire duration of the 50-day observation. The Re and Rg1 mixture induced production of nitric oxide when interacted with RAW 264.7 macrophage cell line. In addition, the mixture caused morphological change of the macrophages. These data indicate that immunostimulation by the Re and Rg1 may be responsible for the protection of mice against disseminated candidiasis.