• Advances in Traditional Medicine
• /
• 제5권1호
• /
• pp.29-36
• /
• 2005

Polysaccharide hydrogels constitute a structurally diverse class of biological macromolecules with a wide range of physicochemical properties. They also constitute important members of the family of industrial water-soluble polymers. They find application in Pharmacy as binders, disintegrants, suspending, emulsifying and sustaining agents. According to the International Pharmaceutical Excipients Council (IPEC), an excipient must have an established safety profile. Hence, in the present study, in vitro cytotoxicity on Vero and HEp-2 cell lines, and in vivo acute toxicity in rats were carried out to establish the safety of polysaccharide hydrogels from the seeds of Plantago ovata and Ocimum basilicum. The in vitro cytotoxicity was determined by MTT and SRB assays. In the in vivo acute toxicity, the effects of three different doses of hydrogels (100, 200 and 400 mg/kg body weight) on food and water intake, body weight, biochemical and hematological parameters were studied. The results of in vitro did not show any cytotoxicity on both the cell lines used. In the in vivo acute toxicity, the hydrogels did not show any toxic symptoms in all three dose levels. This establishes the safety of the selected hydrogels. Hence, they can be used as excipients in pharmaceutical dosage forms.

• Applied Biological Chemistry
• /
• 제41권4호
• /
• pp.273-274
• /
• 1998

In vitro cytotoxicity of sambutoxin was measured by using various human and murine tumor cells and $IC_{50}$ values of sambutoxin ranged from 46.2 to 1,425.6 ng/ml.

• Natural Product Sciences
• /
• 제7권1호
• /
• pp.21-22
• /
• 2001

A cytotoxic compound was purified from the flowers (46 g) of Paulownia tomentosa by normal column chromatography. As a result of the structure analysis by spectroscopic methods, the compound was identified as isoatriplicolide tiglate, which shows in vitro cytotoxicity.

• 약학회지
• /
• 제35권4호
• /
• pp.258-263
• /
• 1991

Seven drivatives of 2, 3-alkyl substituted aziridinecarboximidates were prepared from alkyl substituted alkene and cyanamide. Their ED$_{50}$ values against L$_{1210}$ cell in vitro were evaluated as 1.5 to 4 $\mu\textrm{g}$/ml.

• Archives of Pharmacal Research
• /
• 제22권4호
• /
• pp.380-383
• /
• 1999

Five 1-azzanthraquinone-3-carboxamides were synthesized and evaluated in vitro cytotoxicity against four human cancer cell lines. The most active compound, 7b, exhibited cytotoxic activity comparable to doxorubicin.

• Natural Product Sciences
• /
• 제6권4호
• /
• pp.165-169
• /
• 2000

In vitro cytotoxic activities of cisplatin combined with Chungpesagan-tang or puerarin, which were treated with or without human intestinal bacteria, were measured. When cisplatin was combined with Chungpesagan-tang and its ingredient treated without intestinal bacteria, they did not affect the in vitro cytotoxicity of cisplatin against tumor cell lines. However, when cisplatin was combined with intestinal bacteria-treated Chungpesagan-tang and its ingredients, the cytotoxicities against SNU C4, L1210, A549 and P388 tumor cell lines were synergistically increased. Puerarin, which was isolated from Puerariae Radix, did not show in vitro cytotoxicity. However, its metabolite, daidzein, showed potent cytotoxicity against tumor cell lines and was synergistic by the combined usage of cisplatin. These results suggest that natural glycosides are not only prodrugs which can be transformed to active compounds by intestinal microflora, but the combined usage of cisplatin with natural components, such as daidzein, and herbal medicinal polyprescriptions, such as Chungpesagan-tang, may be a new method for prevention and minimization of the toxicity of cisplatin.

• Environmental Analysis Health and Toxicology
• /
• 제22권3호
• /
• pp.263-269
• /
• 2007

Chitosan is a depolymerized and partially deacetylated derivative of chitin. We investigated the cytotoxicity of chitosan in cancer cell lines, such as P388, L1210, HCT-15, SK-HepG-1 and mouse splenocytes as a normal cell by MTT assay. To clarify whether chitosan enhances cytotoxicity of anticancer drugs, we also examined the cytotoxicity of combined treatment with chitosan and anticancer drugs, such as cisplatin, mitomycin C, and 5-fluorouracil in cancer cell lines in vitro. Chitosan ($37.5\;{\mu}g/mL,\;75\;{\mu}g/mL,\;112.5\;{\mu}g/mL,\;and\;150\;{\mu}g/mL$) showed concentration-dependent cytotoxicity in the cancer cell lines. In addition, chitosan showed relatively lower cytotoxicity in normal cells than in the cancer cell lines. Particularly, this trend was significant at high doses of chitosan, i.e. $112.5\;{\mu}g/mL,\;and\;150\;{\mu}g/mL$. Thus, these results suggest that chitosan may selectively induce the growth inhibition in cancer cell lines, compared to normal cells. Furthermore. the co-treatment of chitosan and anticancer drugs exhibited an apparant synergistic cytotoxicity in murine lymphoma cell lines, i.e. P388 and L1210 at $37.5\;{\mu}g/mL$ of chitosan rather than at $75\;{\mu}g/mL$ of chitosan, but such phenomenon could not be observed in solid tumor cell lines, i.e. HCT-15 and SK-HepG-1. However, chitosan did'nt reduced the cytotoxicity against normal mouse splenocytes induced by anticancer drugs. Therefore, it is concluded that the combination of chitosan and anticancer drugs might be useful for the cancer chemotherapy.

• Journal of Applied Biological Chemistry
• /
• 제58권2호
• /
• pp.113-116
• /
• 2015

Silver materials may be toxic in humans because they can enter the body and accumulate, typically in the lungs. We hypothesized that the cytotoxicity of naive silver materials is affected by their size and shape. Our in vitro assays revealed that the overall toxicity was in the following order: submicro-particles>wires>micro-particles. These results contrast with previous studies, which showed that silver wires are the most toxic among the three tested materials, possibly due to differences in cell lines. Evaluations of in vivo pulmonary toxicity revealed eryptosis in the cavity lining of the lung sections. The observed eryptosis was consistent with the in vitro results. Our results indicate that silver materialinduced cytotoxicity must be measured and compared using various methods.

• 대한치과보철학회지
• /
• 제56권4호
• /
• pp.391-395
• /
• 2018

진료실에서 지르코니아의 수요가 증가하고 있음에 따라 지르코니아의 생체적합성에 대한 평가가 필수적이다. 이번 논문에서는 지르코니아의 생체적 합성에 대한 문헌 중 in vitro 실험에서의 결과를 고찰하였다. Fibroblast, osteoblast, lymphocyte 등 다양한 세포를 이용한 in vitro 실험에서 지르코니아 블록은 뛰어난 생체적합성을 보였다. 여러 연구에서 지르코니아에 대한 세포독성 및 돌연변이 유발이 관찰되지 않았으며, 세균부착도 적은 것으로 나타났다. 장기간 체액노출조건을 모방한 in vitro 실험에서는 약간의 기계적 강도의 감소 외에는 악영향이 관찰되지 않았다. Osteoblast-like cell을 이용한 연구에서, 지르코니아 블록이 면역반응, 물질이동, 세포주기조절에 관여하는 유전자를 조절하여 생체적합을 높이는 것으로 관찰되었다. 여러 in vitro 연구에서 지르코니아 분말의 생체적합성에 대해서는 상이한 보고가 나타나고 있다. 종합적으로 지르코니아 분말은 어느 정도 세포독성을 가지고 있는 것으로 생각된다.

• 대한화장품학회:학술대회논문집
• /
• 대한화장품학회 1992년도 자외선 차단 화장품의 SPF에 관한 심포지움(대한화장품학회)
• /
• pp.46-68
• /
• 1992

To investigate the effect on the antimicrobial activity against S.aureus ATCC 6538, E.coli KCTC 1039 and cell toxic level against transformed mouse fibroblast L929 in formula added with various concentrations of UVB blockers commonly used in cosmetic products, these experiments were carried out by preservative efficacy testing methods and in vitro cytotoxicity methods. The results obtained were as follow ; 1) Octyl Dimethyl PABA had a broad antibacterial spectrum against the Gram (+) and the Gram(-) bacteria at 5.84 % concentration, but not Octyl Methoxycinnamate. 2) Antibacterial activity was decreased in a combined UVB blocker system of squalane base. Especially, Octyl Dimethyl PABA was inactivated by Octyl Methoxycinnamate at 5.84% concentration to a large extents , but not 4-Methylbenzylidene Camphor. 3) Within in vitro cytotoxicity by use of mouse fibroblast L929 on UV-B blockers, NR assay was more excellent than MTT assay on quantitative