• Title/Summary/Keyword: incorporation efficiency

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Investigation of the Incorporation Efficiency of $\beta$-Carotene into Liposomes

  • Rhim, Chae-Hwan;Lee, Kyong-Eun;Yuk, Hyun-Gyun;Lee, Sang-Chun;Lee, Seung-Cheol
    • Preventive Nutrition and Food Science
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    • v.5 no.3
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    • pp.177-178
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    • 2000
  • Chemical and photochemical precesses during food storage an preparation rapidly degrade $\beta$-carotene, the most active form of carotenoids. We investigated the possibility of liposomes as tool to preserve $\beta$-carotene. Liposomes with $\beta$-carotene were prepared as multilamellar vesicles by using soybean phosphatidylcholine, in terms of the ratio of $\beta$-carotene to phospholipid and pH. Incorporated efficiency was 99.7% at 1:0.05 of phospholipid : $\beta$-carotene and at pH 9.0. As the concentration of $\beta$-carotene increased, the incorporated efficiency increased progressively. pH did not affect the incorporation efficiency greatly.

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MOCVD Growth of AlGaAs/InGaAs/GaAs Pseudomorphic Structures and Transport Properties of 2DEG (AlGaAs/InGaAs/GaAs Pseudomorphic 구조의 MOCVD 성장 및 2차원 전자가스의 전송특성)

  • 양계모;서광석;최병두
    • Journal of the Korean Vacuum Society
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    • v.2 no.4
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    • pp.424-432
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    • 1993
  • AlGaAs/InGaAs/GaAs pseudomorphic structures have been grown by atmosheric pressure-MOCVD . The Al incorporation efficiency is constant but slightly exceeds the Ga incorporation during the growth of AlGaAs layers at $650^{\circ}C$ . Meanwhile , the In incorporation efficiency is constant but slightly less than the Ga incorporation in InGAAs layers. InGaAs/GaAs QWs were grown and their optical properties were characterized . $\delta$-doped Al0.24Ga0.76As/In0.16 Ga0.84As p-HEMT structures were successfully grown by MOCVD and their transport properties were characterized by Hall effect and SdH measurements. SdH Measurements at 3.7K show clear magnetoresistance oscillations and plateaus in the quantum Hall effect confirming the existence of a two-dimensional electron gas(2DEG) and a parallel conduction through the GaAs buffer layer. The fabricated $1.5\mu\textrm{m}$gatelength p-HEMTs having p-type GaAs in the buffer layer show a high transconductance of 200 mS/mm and a good pinch-off characteristics.

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Effect of Substitution of Groundnut with Soybean Meal at Varying Fish Meal and Protein Levels on Performance and Egg Quality of Layer Chickens

  • Naulia, Uma;Singh, K.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.11
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    • pp.1617-1621
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    • 2002
  • Two hundred and sixteen single comb white egg layers of the White Leghorn hens of 24 weeks of age were randomly allocated to 12 groups with three replications of six hens in each. Hens were fed in a factorial arrangement 2${\times}3{\times}$2, on diets containing either 16 or 18% crude protein with 0, 3 or 6% fish meal, replacing groundnut meal with soybean meal. Soybean meal incorporation improved (p<0.05) egg production, feed intake, feed conversion efficiency and egg weights. Egg quality traits of specific gravity, shape index, albumen index, yolk index and shell thickness remained unchanged. Laying performance was significantly (p<0.05) better at 18% than on 16% dietary protein level. Use of fish meal linearly improved egg production and feed conversion efficiency on diets supplemented with groundnut meal and fish meal incorporation showed quadratic improvement on feed conversion efficiency with SBM diets at 16% dietary protein level. Therefore, use of soybean meal as substitute of groundnut meal is recommended in layer diets, at 16% dietary protein level and fish meal incorporation could be beneficial for layers.

Effect of ${\beta}$-Sitosterol in Liposome Bilayer on the Stabilization of Incorporated Retinol

  • Lee, Seung-Cheol;Kim, Jin-Ju;Lee, Kyung-Eun
    • Food Science and Biotechnology
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    • v.14 no.5
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    • pp.604-607
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    • 2005
  • In this study, the effect of ${\beta}$-sitosterol (SS) in the liposome bilayer on the stability of incorporated retinol was evaluated. Retinol was incorporated into liposomes consisting of various ratios of soybean phoaphatidylcholine (PC) to SS, while liposomes were prepared as multilamellar vesicles by the dehydration/rehydration method. Retinol was readily incorporated into liposomes with various SS contents, with incorporation efficiencies higher than 98% for all conditions. The incorporation efficiency of retinol increased slightly as the SS content in liposomes increased. Its average particle size also increased as the SS content increased. Mean particle size at PC to SS ratios of 100:0, 90:10, 80:20, 70:30, 60:40, and 50:50 were 12.16, 17.57, 35.00, 40.62, 83.45, and $88.94\;{\mu}m$, respectively. Liposomal retinol degradation in aqueous solution was measured with respect to SS content at various periods of time at four different temperatures of 4, 25, 37, and $50^{\circ}C$, and the stability of the incorporated retinol enhanced as the SS content increased. At $4^{\circ}C$, for example, retinol in the liposomes of 50:50 (PC:SS) remained at 84.42% after storage for 10 days, while in 100:0 (PC/SS) it remained at 42.62%. These results indicate that SS content in liposomes played an important role in the incorporation efficiency of retinol and its stability.

Genetic Incorporation of a Phenanthroline-Containing Amino Acid in Escherichia coli

  • Jin, Sunhwa;Lee, Hui-Jung;Lee, Sangyeul;Lee, Hyun Soo
    • Bulletin of the Korean Chemical Society
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    • v.35 no.4
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    • pp.1087-1090
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    • 2014
  • A simple and general method that selectively introduces metal binding sites into a protein can greatly increase the ability to design and biosynthesize artificial metalloproteins. Here, we report the incorporation of a phenanthroline-containing amino acid (Phen-Ala) into proteins in Escherichia coli by using the $tRNA{^{Tyr}}_{CUA}$ and tyrosyl aminoacyl-tRNA synthetase pair (BpyRS) from Methanococcus jannaschii, which was originally developed for a bipyridine-containing amino acid (Bpy-Ala). The incorporation efficiency of BpyRS for Phen-Ala was comparable to that for Bpy-Ala. Because of its high metal-binding ability and characteristic spectral properties, Phen-Ala can be a useful alternative to the existing metal-chelating amino acids for the design and synthesis of artificial metalloproteins.

Effect of cholesterol into liposome on the stabilization of incorporated retinol

  • Lee, Jae-Uk;Lee, Soo-Jin;Kang, Joo-Sung;Lee, Kyung-Eun;Kim, Jin-Ju;Lee, Seung-Cheol
    • Proceedings of the SCSK Conference
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    • 2003.09b
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    • pp.60-72
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    • 2003
  • To investigate the effect of cholesterol in liposome on the stability of incorporated retinol, the physico-chemical experiments for various amounts of cholesterol-containing liposomes were performed. Liposome with retinol containing cholesterol was prepared as multilamella vesicles(MLVs) by dehydration/rehydration method. The incorporation efficiency of retinol into liposome was maximized as 99.31 % at 50:50 (phosphatidylcholine/cholesterol) at pH 9. The stability of incorporated retinol at low storage temperature was enhanced with increasing cholesterol content than at high storage temperature. For example, incorporated retinol in liposome at glycine buffer(pH 9} was degraded slowly during storage at 4. The degradation of retinol in liposomes was slower at pH 9 than at pH 7. These results supported that cholesterol in liposome increased largely the stability of incorporated retinol.

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Stabilization of Retinol through Incorporation into Liposomes

  • 이승철;육현균;이동훈;이경은;황용일;Richard D. Ludescher
    • BMB Reports
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    • v.35 no.4
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    • pp.358-363
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    • 2002
  • Chemical and photochemical processes during storage and preparation rapidly degrade retinol, the most active form of vitamin A. therefore, the efficacy of incorporation into liposomes in order to modulate the kinetics of retinol degradation was investigated. Retinol was readily incorporated into multilamellar liposomes that were prepared form soybean phosphatidylcholine; the extent of the incorporation was 98.14±0.93% at pH 9.0 at a ratio of 0.01 : 1 (wt:wt) retinol : phospholipid. It was only marginally lower at higher retinol concentrations. The pH of the hydration buffer had a small effect. The incorporation efficiency ranged from 99.25±0.47% at pH 3 to 97.45±1.13% at pH 11. The time course of the retinol degradation in the aqueous solution in liposomes was compared to that of free retinol and free retinol with α-tocopherol under a variety of conditions of pH(3, 7, and 11), temperature(4, 25, 37, and 50℃), and light exposure(dark, visible, and UV). The retinol that was incorporated into the liposomes degraded significantly slower than the free retinol or retinol with α-tocopherol at pH 7 and 11. At pH 3, where the free retinol degrades rapidly, the degradation kinetics were similar in liposomes and the presence of α-tocopherol. At pH 7.0 and 4℃ in the light, for example, free aqueous retinol was completely degraded within 2 days, while only 20% of the retinol in the liposomes were degraded after 8 days. In general, the protective effect of the liposome incorporation was greater at low temperatures, at neutral and high pH, and in the dark. The results suggest that protection is greater in the solid, gel phase than in the fluid liquid crystalline phase lipids. These results indicate that the incorporation into liposomes can extend the shelf-life of retinol under a variety of conditions of temperature, pH, and ambient light conditions.

Metal-Urea-Montmorillonite Hybrid Incorporated with Citric Acid

  • Kim, Kwang Seop;Kim, Min-Tae;Ryu, Jin-Hee;Choi, Jong-Seo;Park, Ki Do;Kang, Hang-Won;Park, Man
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.6
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    • pp.610-614
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    • 2013
  • Massive intercalation of urea into montmorillonite (MUCH) was recently proposed to enhance urea use efficiency through smart suppression of emission of $NH_3$ and NOx. This study was to synthesize citrate-incorporated MUCH (Cit-MUCH) which can enhance suppression of $NH_3$ volatilization. The XRD pattern of Cit-MUCH was very similar to that of MUCH to indicate successful incorporation of citric acid into MUCH. Incorporation of citric acid was confirmed by the existence of $COO^-$ symmetric stretching vibration. During the initial 4 days after application, $NH_3$ volatilization from both bare and perilla-planted soils was much more suppressed by application of Cit-MUCH than MUCH. A peak volatilization rate decreased from 28.3 N mg $m^{-2}\;h^{-1}$ of MUCH-broadcasted soil to 22.2 N mg $m^{-2}\;h^{-1}$ of Cit-MUCH-broadcast soil. $NH_3$ volatilization was less in planted soil than bare soil for 72 hrs after application. These results showed that incorporation of citric acid led to increase in suppression of ammonia volatilization from urea-applied soils.

Do customized orthodontic appliances and vibration devices provide more efficient treatment than conventional methods?

  • Aldrees, Abdullah M.
    • The korean journal of orthodontics
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    • v.46 no.3
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    • pp.180-185
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    • 2016
  • The incorporation of technological advances in the field of clinical orthodontics to increase treatment efficiency has led to the development of customized appliances (Insignia$^{(R)}$), archwires (Suresmile$^{(R)}$), and the production of devices to enhance tooth movement (Acceledent$^{(R)}$). This review presents a comprehensive study of the literature concerning these products, and analyzes the available evidence of their efficiency. To date, one pilot study has evaluated the efficiency of the Insignia$^{(R)}$ system, three retrospective studies have assessed the efficiency of the Suresmile$^{(R)}$ system, and a few Acceledent$^{(R)}$ reports have described its effect on treatment time. Critical appraisal of the reviewed papers revealed that the efficiency of the Insignia$^{(R)}$ system cannot be confirmed based on the available evidence, while the use of Suresmile$^{(R)}$ can reduce overall treatment time in simple cases. The acceleration of tooth movement by Acceledent$^{(R)}$ devices has not yet been confirmed.

PVA Fiber Incorporation Effect According to Light Transmission Direction of LEFC (빛 투과 방향에 따른 LEFC의 PVA섬유 혼입 효과)

  • Seo, Seung-Hoon;Kim, Tae-Wan;Kang, Young-Un;Jeon, Seung-Heon;Oh, Sang-Kun;Kim, Byoung-Il
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2018.05a
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    • pp.17-18
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    • 2018
  • With the technological advancement of the construction industry, construction materials with future direction appeared. In Korea, LEFC(Light Emotion Friendly Concrete) has been developed and studied, which has improved labor productivity and economic efficiency over semi-transparent concrete by introducing transparent plastic rods into concrete matrix. However, there is a problem in that the bending performance is lowered according to the insertion of the rod and the bending performance is different according to the light transmission direction. In this study, the incorporation of PVA fibers in the direction of light transmission was increased and the change of flexural performance according to the interval of light transmission was examined.

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