• Title/Summary/Keyword: indicator organism

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A study on the Yersinia Isolated from Spring Water in Northern Area of Seoul (서울 북부 지역 옹달샘에서 분리한 Yersinia 속균에 관한 연구)

  • 변신철;노우섭
    • Journal of Environmental Health Sciences
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    • v.24 no.1
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    • pp.141-150
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    • 1998
  • This study was performed to investigate the distribution of Yersiniae and correlation between Yersiniae and indicator organism by time and area in spring water located in northern part of Seoul. Samples collected from 46 spring waters located in four mountains(Dobong, Bukhan, Surak, Bulam) were inspected to detect Yersiniae and indicator organisms. And also there were examined bioserological characteristics and resistance of ahtibiotics of the isolated Yersiniae.The result were as follows. 1. The isolation rate of Yersiniae was 22% in February and 20% in April. The isolated species were 6 strains of Y. enterocolitica, 6 strains of Y. aldova, 4 strains of Y. intermedia and 43 strains of Y. pseudotuberculosis. The serotype of Y. pseudotuberculosis isolated from was all O:5 and biotype of Y. enterocolitica isolated from was all O:3. 2. The Geometric mean of standard plate count, coliform, and psychrotrophilic bacteria were 3.4 CFU/ml, 1.2 MPN/100 ml and 33.0 CFU/ml in February and 3.1 CFU/ml, 1.5 MPN/100 ml and 20.5 CFU/ml in April respectively. There was no significant difference by time and area but the indicator organisms were correlated significantly with each other (p<0.05). 3. Because detection of Yersiniae was not statistically associated with indicator organism, Yersiniae can be detected in the spring water approved microbiologically (p<0.05). 4. The Yersiniae isolated were resistant to Ampicillin, Colistin, Carbenicillin and Coilstin. All isolaed Y. enterocolitica were resistant to Ampicillin (100%). In the case of Y. pseudotuberculosis, only 1 of 3 isolated was resistant to Colistin but susceptible to other antibiotics.

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Marine Enviromental Pollution Studies Using Bivalves as a Bioindicator (이매패류를 지표종으로 이용한 해양환경오염 연구)

  • 이수형
    • The Korean Journal of Malacology
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    • v.10 no.2
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    • pp.24-40
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    • 1994
  • Marine environmintal pollution studies using bivalves as an indicator organism are described. The utility of the' Mussil Watch in providing a measure of environmental persistent pollutants is also reviewed. Finally, the data from the korea Mussel Watch in 1990 are prisented and compared with those from other regions in the world.

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Disinfection Effect of Chlorine, Chlorine Dioxide end Ozone on Total Coliform in Water

  • Lee, Yoonjin;Kyoungdoo Oh;Byongho Jun;Sangho Nam
    • Proceedings of the Korean Environmental Health Society Conference
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    • 2003.06a
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    • pp.140-143
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    • 2003
  • This research was to determine and compare the inactivation of total coliform as the indicator organism with chlorine, chlorine dioxide and ozone for drinking water treatment. The inactivation of total coliform was experimentally analyzed for the dose of disinfectant, contact time, pH, Temperature and DOC. The experiments for the characterization of inactivation were performed in a series of batch processes with the total coliform as a general indicator organism based on chlorine, chlorine dioxide and ozone as disinfectants. The nearly 2.4, 3.0, 3.9 log inactivation of total coliform killed by injecting 1mg/L at 5 minutes for chlorine, chlorine dioxide and ozone. For the inactivation of 99.9%, Disinfectants required were 1.70, 1.00 and 0.60 mg/L for chlorine, chlorine dioxide and ozone, respectively. The bactericidal effects of disinfectants were decreased as the pH increased in the range of pH 6-9. The influence of pH change on the killing effect of chlorine dioxide was not strong, but that on ozone and free chlorine was sensitive. The bactericidal effects of the disinfectants were increased as the temperature increase. The activation energies were 36,053, 29,822, 24,906 J/mol of chlorine, chlorine dioxide, ozone for coliforms. The inactivation effects were shown in the lowest order of chlorine, chlorine dioxide and ozone.

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Reliability Assessment of Temperature Indicator for Simplified Measurement on Conservation Environment of Cultural Heritage (문화재 보존환경 간이 측정을 위한 온도지시카드 신뢰성 평가)

  • Lim, Bo-A;Shin, Eun-Jeong;Lee, Sun-Myung
    • 보존과학연구
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    • s.31
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    • pp.59-68
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    • 2010
  • Cultural heritages are damaged by surrounding several environmental factors. Main factors are temperature, humidity, light, atmosphere and indoor pollutant, organism, etc. Therefore, to prevent damage of cultural heritage from such environmental factor, conservation environment monitoring becomes more important. Indicator is one of the simple method for environment monitoring. It can be used without expensive and complex equipments. However, it should be performed scientific examination for application to cultural heritage. In this study, some Temperature Indicators were chosen and reliability assessment was carried out for application to cultural heritage. Brightness($L^*$) is selected for reliability assessment factor. As a result of lab test, Temperature Indicators were not influenced greatly in humidity change. When they were exposed to setting temperature, the color was changed in setting temperature area and ${\pm}2^{\circ}C$ part of setting temperature. Especially brightness value was high in setting temperature area. Also, Temperature Indicators were stabilized after about 16 minutes when were exposed to temperature difference of $10^{\circ}C$ and when temperature difference with exposure environment is smaller, stabilization time shortened. Therefore, it is a possible to confirm that selected Temperature Indicator is reliable product through measurement of color difference value and naked eye observation.

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A Study on Indicator Bacteria for Water Quality Management of Urban Artificial Lakes (도심지역 인공호의 수질관리를 위한 지표세균에 관한 연구)

  • Chu, Duk-Sung;Kwon, Hyuk-Ku;Lee, Sang-Eun;Lee, Jang-Hoon
    • Journal of Environmental Health Sciences
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    • v.33 no.4
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    • pp.299-305
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    • 2007
  • Distribution of fecal pollution indicator bacteria and environmental parameter were investigated of urban artificial lakes. An average concentration of temperature, pH, SS, DO, $COD_{Mn}$, T-P, T-N, Turbidity, Chl-a were $21.5^{\circ}C$, 8.07, 116.70 mg/l, 8.66 mg/l, 2.24 mg/1, 0.52 mg/l, 1.71mg/l, 80.54 NTU, and 52.12 mg/l respectively. From the results of bivariate correlation analysis, fecal contamination indicator bacteria were found to be mutually correlated. And turbidity and suspended solid were correlated. From the results of principal component analysis, four factors were extracted. And four factors of variance explained up to 81.5 percentage. Factor 1 was pollution pattern by fecal contamination, factor 2 was physical pollution pattern by pollution source, factor 3 was natural pollution by precipitation, and factor 4 was artificial pollution pattern by organism.

Use of the Comet Assay to Assess DNA Damage in Hemocytes and Gill of Oyster(Crassostrea gigas) Exposed to Pyrene and Benzo(a)pyrene (Pyrene과 Benzo(a)pyrene에 노출된 굴의 혈구세포과 아가미 세포에서의 DNA손상 측정을 위한 Comet assay의 이용)

  • 김기범;배세진
    • Journal of Aquaculture
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    • v.16 no.3
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    • pp.196-201
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    • 2003
  • Sessile organisms such as the oyster Crassostrea gigas have been given much attention as a potential biomonitoring indicator to assess the impact of toxicants on aquatic organism. In this study, we exposed cells isolated from gill of oyster (Crassostrea gigas) to hydrogen peroxide in vitro. In addition oysters were in vivo exposed to pyrene and benzo(a)pyrene at various concentrations for 2 weeks. Comet assay was used to detect DNA single strand breaks and to investigate the application of this technique as a tool for aquatic biomonitoring. Hydrogen peroxide increased DNA single strand break with increasing concentration after 30 minutes exposure in vitro. Pyrene and benzo(a)pyrene caused DNA damage only at very high concentration (100 $\mu\textrm{g}$/L or 1000 $\mu\textrm{g}$/L) at two week exposure in vivo. DNA damage was relatively higher at hemocyte than at gill. It suggested that metabolized PAHs are transferred to hemolymph from digestive gland which have a relatively high enzyme activity, and attacked the DNA of hemocyte, while gill accumulated PAHs without degrading them to their metabolites due to low enzyme activity at gill. Both in vitro and in vivo exposure experiments showed that the comet assay is an effective tool on screening whether the organism are exposed to genotoxic contaminants.

Distribution of Indicator Organisms and Incidence of Pathogenic Bacteria in Raw Beef Used for Korean Beef Jerky

  • Kim, Hyoun-Wook;Kim, Hye-Jung;Kim, Cheon-Jei;Paik, Hyun-Dong
    • Food Science and Biotechnology
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    • v.17 no.6
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    • pp.1337-1340
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    • 2008
  • The objective of this study was to evaluate the microbial safety of raw beef used to produce Korean beef jerky, The raw beef samples harbored large populations of microorganisms. In particular, psychrophilic bacteria were found to be most numerous ($9.2{\times}10^3-1.0{\times}10^5\;CFU/g$) in the samples. Mesophilic bacteria and anaerobic bacteria were present in average numbers ($10^3-10^5\;CFU/g$). Spore-forming bacteria and coliforms were not detected below detection limit. Yeast and molds were detected at $2.2{\times}10^1-7.8{\times}10^2\;CFU/g$ in the raw beef. Ten samples of raw beef were analyzed for the presence of pathogenic bacteria. Bacillus cereus was isolated from sample B, G, and H. The B. cereus isolates from raw beef samples were identified with 99.8% agreement according to the API CHB 50 kit.

Relationship between Physical Environmental Factors and Biological Indices of A Mountain Valley Stream (Mt. Cheoggye) (산간계류(청계산)의 물리적 환경요인과 생물지수의 관계)

  • Minjeong Yeo;Dongsoo Kong
    • Journal of Korean Society on Water Environment
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    • v.39 no.4
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    • pp.288-301
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    • 2023
  • This study aims to identify benthic macroinvertebrate fauna inhabiting at the mountain valley stream (Mt. Cheonggye) in Korea and the relationship between physical environmental factors and biological indices. Benthic macroinvertebrates were collected at five locations on August 24 and October 14, 2020, and were identified as 4 phyla, 7 classes, 16 orders, 42 families, and 72 species. Dominance ranged from 0.38 to 0.59, diversity 2.81 to 3.75, richness 3.25 to 4.63, evenness 0.65 to 0.84, and %EPT (Ephemeroptera-PlecopteraTrichoptera) richness value 42% to 73%, respectively. All sites were evaluated as a very good status by mostly biological indices based on tolerance of indicator organisms in Korea. As a result of principal component analysis, biological indices are classified into species-level indices and higher cartegory-level indices according to the taxonomic level of the indicator organism considered in each index. As a result of canonical correspondence analysis, it was confirmed that current velocity was a major factor that increased species richness and classified biological indices according to taxonomic category level. Water depth was a major factor related to the community indices, and the deeper the water depth, the lower the diversity and the evenness.

Distribution of Indicator Organisms and Influence of Storage Temperature and Period in Commercial Plant Food (시판 식물성 식품의 오염지표세균 분포 및 저장온도, 기간별 오염지표세균의 변화)

  • 이용욱;박석기
    • Journal of Food Hygiene and Safety
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    • v.14 no.1
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    • pp.1-8
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    • 1999
  • There were few data for the distribution of the indicator organisms in the commercial plant foods, and for the normal flora and for the foodborne agents within the country. First of all it must be investigated the distribution of the indicator organisms. And also it is very important to prepare the sanitation criteria for the plant foods through the microbiological examination and the investigation of tendency to change of the indicator organisms according to the storage temperature and period. The average number of total viable counts for grains was 2.9$\times$105/g, psychrophilic bacteria 2.9$\times$105/g, heterotrophic bacteria 3.1$\times$105/g, heat-resistant bacteria 2.1$\times$103/g, Pseudomonas aeruginosa 23/g. That for beans was 6.3$\times$102/g, psychrophile 34/g, heterotroph 1.7$\times$102/g. That for sesames was 1.4$\times$105/g, coliform 350/g, psychrophile 7.4$\times$104/g, heterotroph 5.8$\times$104/g, Pseud. aeruginosa 2.3$\times$103/g. heat-resistant bacteria 150/g. That for potatoes was 2.0$\times$107/g, coliform 5.0$\times$104/g, psychrophile 1.8$\times$107, heterotroph 1.4$\times$107/g, heat-resistant bacteria 3.3$\times$104/, Staphylococcus 2.7$\times$105/g, fecal streptococcus 4.5$\times$103/g, Pseud. aeruginosa 7.0$\times$103/g. That for mushrooms was 1.2$\times$108/g, psychrophile 9.4$\times$107/g, heterotroph 1.0$\times$109/g, heat-resistant bacteria 1.6$\times$105/g, Pseud. aeruginosa 1.3$\times$103/g. That for vegetables was 5.9$\times$1011/g, coliform 1.8$\times$106g/, Staphylococcus 1.1$\times$1012/g, heterotroph 8.4$\times$1011/g, heat-resistant bacteria 7.6$\times$106/g, Staphylococcus 1.1$\times$107/g, fecal streptococcus 1.1$\times$104/g, Pseud. aerugniosa 5.2$\times$104/g. That for nuts 3.9$\times$104/g, coliform 3.9$\times$103/g, psychrophile 4.0$\times$104/g, heterotroph 3.2$\times$104/g, heat-resistant bacteria 400/g. In commercial grains and beans, SPC, psychrophile, heterotroph and heat-resistant bacteria stored at 1$0^{\circ}C$, 2$0^{\circ}C$, 3$0^{\circ}C$ were constant. Staphylococcus, coliform, Pseud. aeruginosa were decreased a little n grains, but were not detected in beans. In mushrooms, all indicator organisms were increased as time goes on and were increased rapidly at 2$0^{\circ}C$. In sesames, coliform was not detected at all temperature. psychrophile was increased for 7 days, the others were constant. In potatoes, SPC, psychrophile, heat-resistant bacteria, heterotroph had a tendency to increase and the others were constant. In vegetables, indicator organisms were had a tendency to increase, psychrophile, heterotroph were rapidly increased after 7 days. In nuts, SPC, coliform, psychrophile heterotroph, heat-resistant bacteria, Pseud. aeruginosa were constant, staphylococcus and fecal streptococcus were not detected.

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A Study of Antimicrobial Resistance in Escherichia coli and the Distribution of Indicator Microorganisms in Asan City (아산시 지표미생물의 분포와 Escherichia coli의 항생제 내성에 관한 연구)

  • Lee, Geun-Yeol;Kim, Keun-Ha;Kwon, Mun-Ju;Kwon, Hyuk-Ku;Kim, Yeon-Hee;Lee, Jang-Hoon
    • Journal of Environmental Health Sciences
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    • v.36 no.3
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    • pp.229-235
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    • 2010
  • Efforts to evaluate water pollution using indicator microorganisms have been underway for decades, and driven by research on water purity control applications, water quality criteria are growing more and more strict. Furthermore, recent reports indicate that high concentrations of antibiotics are not absorbed, and are present in excrement from animals and humans dosed with unnecessarily high levels of antibiotics. This has emerged as very important issue from the standpoint of being an ecological and health hazard. In this study, water pollution was analyzed through physicochemical and microbiological means, and antibiotic resistance in indicator microorganisms was assessed. In physicochemical analysis, biochemical oxygen demand (BOD)$_5$ and chemical oxygen demand (COD)$_{Mn}$ evaluation showed that pollution by organisms was highest at the G1 location with a high human population, and the DP location which has many livestock-containing households. The indicator organism levels at the G1 location were: Total Coliforms (1205 colony forming units (CFU)/100 ml), Fecal Coliforms (270 CFU/100 ml), Escherichia coli (253 CFU/100 ml) and Fecal Streptococci (210 CFU/100 ml), while for the DP location levels were: Total Coliforms (1480 CFU/100 ml), Fecal Coliforms (438 CFU/100 ml), E. coli (560 CFU/100 ml), and Fecal Streptococci (348 CFU/100 ml). Levels of fecal indicator microorganisms such as Fecal Coliforms, E. coli and Fecal Streptococci were high at all locations in the fall (the period after the rainy season), and the yearly distribution was similar between these organisms. If the number of livestock-containing households was high, almost all strains of E. coli (as distinct from the other indicator organisms) showed resistance to antibiotics, with the degree of resistance varying between areas. E. coli strains from the OY area in particular, which has a high population density, showed strong resistance to AM10 and Va30. While strong antibiotic resistance was observed overall at the DP and OY locations, no resistance was observed at the EB location.