• 대한지역사회영양학회지
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• 제4권2호
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• pp.239-247
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• 1999

Six-week-old Sprague Dawley rats were fed the diets of 20% casein or soy protein. Two weeks after the feeding, hepatocellular chemical carcinogenesis was initiated by diethylnitrosamine(DEN), and promoted by the diet containing 0.01% 2-acetylamino-fluorene(AAF) and two-thirds partial hepatectomy(PH). The animals were sacrificed at 8 weeks after the DEN injection. The area of placetal glutathione S-trnasferase(GST-P) positive foci, the activities of several enzymes in cellualr antioxidant enzyme systems and glucose 6-phosphatase were determined to investigate the mechanism of the anticarcinogenic effect by the dietary proteins. In another set of experiments, the drinking water of rats fed casein was supplemented with 1.5% inositol hexaphosphate(InsP6) to elucidate whether it has the comparable anticancer action of soy protein. The area and number of GST-P positive foci in the soy protein group were significantly(p<0.05) lower than those inthe casein group. The livers of rats fed casein showed moderate fattydegeneration and larger hyperplastic nodules than those of rats fed soy protein. In another set of experiments, the area and number of GST-P positive foci in the rats fed casein supplemented with InsP6 were not significantly different from those in the rats fed casein or soy protein. The lipid peroxidation of rats fed different protein sources showed no significant difference. Glutathione S-transferase(GST) activities were increased significantly(p<0.05) by carcinogen treatment in all dietary groups. Glucose 6-phosphatase(G6Pase) activities were decreased by carcinogen treatment, and hence showed a reverse relationship(r=-0.695, p<0.01) to the GST-P positive foci. Therefore, the activities in the rats fed casein were lower than those in the rats fed soy protein. These results suggest that the soy protein seems to be more anti-carcinogenic than casein by decreasing the preneoplastic lesion and by increasing the membrane stability but inositol hexaphosphate, a component of soy protein, may not be protective against hepatocarcinogenesis.

• 대한지역사회영양학회:학술대회논문집
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• 대한지역사회영양학회 1998년도 춘계학술대회:경제위기시대의 합리적인 식생활
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• pp.81-81
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• 1998

• 대한가정학회:학술대회논문집
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• 대한가정학회 2005년도 제58차 정기총회 및 추계학술대회
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• pp.117-117
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• 2005

• Nutrition Research and Practice
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• 제1권3호
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• pp.195-199
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• 2007

Inositol hexaphosphate ($IP_6$) is a major constituent of most cereals, legumes, nuts, oil seeds and soybean. Previous studies reported the anticancer effect of $IP_6$ and suggested that co-treatment of $IP_6$ with inositol may enhance anticancer effect of $IP_6$. Although the anticancer effect of $IP_6$ has been intensively studied, the combinational effect of $IP_6$ and inositol and involved mechanisms are not well understood so far. In the present study, we investigated the effect of $IP_6$ and myo-inositol (MI) on cell cycle regulation and apoptosis using PC3 prostate cancer cell lines. When cell, were co-treated with $IP_6$ and MI, the extent of cell growth inhibition was significantly increased than that by $IP_6$ alone. To identify the effect of $IP_6$ and MI on apoptosis, the activity of caspase-3 was measured. The caspase-3 activity was significantly increased when cells were treated with either $IP_6$ alone or both $IP_6$ and MI, with no significant enhancement by co-treatment. To investigate the effect of $IP_6$ and MI of cell cycle arrest, we measured p21 mRNA expression in PC3 cells and observed significant increase in p21 mRNA by $IP_6$. But synergistic regulation by co-treatment with $IP_6$ and MI was not observed. In addition, there was no significant effect by co-treatment compared to $IP_6$ treatment on the regulation of cell cycle progression although $IP_6$ significantly changed cell cycle distribution in the presence of MI or not. Therefore, these findings support that $IP_6$ has anticancer function by induction of apoptosis and regulation of cell cycle. However, synergistic effect by MI on cell cycle regulation and apoptosis was not observed in PC3 prostate cancer cells.

• 한국축산시설환경학회지
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• 제7권2호
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• pp.83-102
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• 2001

Phytic acid (myo-inositol hexaphosphate or IP6) is the major storage form of phosphorus in cereals and legumes, representing 18 to 88% of the total phosphorus. Phytate form of phosphorus is not readily utilized by monogastric animals and this result causes pollution problem by phosporus released in areas of intensive livestock production. The interaction between phytic acid and essential dietary minerals, protein, or vitamins is considered to be one of the primary factors limiting the nutritional values of cereals and legunes in monogastric animals. Attempts have been made to hydrolyze dietary phytic acid by phytases to improve the feed quality and to decrease the amount of phosphorus excreted by animals. Phytase(myo-inositol hexakisphosphate phosphohydrolase) hydrolyzes phytic acid to myo-inositol and phosphoric acid. Two types of phytases are known: 3-phytase (EC 3.1.3.8) and 6-phytase (EC 3.1.3.26), indicating the intial attack to the susceptable phosphoester bond. Because of its great industrial importance, there is ongoing interest in isolating new bacterial strains producing novel and efficient phytases.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1996년도 한국생물과학협회 국제학술대회
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• pp.182.1-182
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• 1996

No Abstract, See Full Text

• Asian-Australasian Journal of Animal Sciences
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• 제8권2호
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• pp.135-138
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• 1995

Soybean meal was fermented by Aspergillus usami in order to reduce phytate content. Aflatoxin B1 was not detected in the fermented soybean meal. The contents of crude protein, crude fiber, ether extract and crude ash were slightly increased following fermentation with a concomitant reduction in nitrogen free extract. Though the fermentation partly degraded proteins in the soybean meal, there was small difference in amino acid composition between the soybean meal and the fermented soybean meal. The results showed that the fermentation did not affect nutritional value of protein in soybean meal. Approximately 55% of phosphorus extracted by trichloroacetic acid was inositol hexaphosphate (phytate) in the soybean meal. The content of inositol tetra to hexaphosphates was not detected in the fermented soybean meal. These results indicated that the fermentation almost completely eliminated phytate in soybean meal. Phytase activity was not detected in the unfermented soybean meal. However, the enzyme activity in the fermented soybean meal was 167.7 U/g. When the fermented soybean meal in supplemented in formula feeds, phytase in the fermented soybean meal might partly degrade the phytate in other ingredients in the digestive tract. The fermented soybean meal is possibly used as a phytate-free protein source of feed, which contains high available phosphorus.

• 한국환경보건학회지
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• 제27권4호
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• pp.1-8
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• 2001

From August 2000 to August 2001, 9 variables of physicochemical factors and phytase activity were investigated at 4 sites in the River Yungpyung and the influences of Physicochemical factors to Phytase activity were analyzed. Phytase activities of Site 1, Site 2, Site 3, and Site 4 varied between N.D ∼566 nmol/ ι /hr, N.D \" 434 nmol/ ι /hr, N.D ∼557 nmol/ ι /hr, and N.D ∼723 nmol/ ι /hr, respectively. The activities of summer season were higher than those of other season. But the activities were not detected on the winter season. The phytase activity and temperature showed high correlation. The correlation coefficients of Site 1, Site 2, Site 3, and Site 4 were 0.82(p<0.01).0.92(p<0.01),0.87(p<0.01), and 0.88(p<0.01), respectively. The phytase activity and NOI₃/sup -/ ion showed negative relation(r=-0.59, p<0.05) at Site 1. And the phytase activity had relation with Zn/sup 2+/at Site 2(r=().57, p<0.05) and Site 3(r=0.7E, p<7.07).

• 한국식품위생안전성학회지
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• 제22권4호
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• pp.382-387
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• 2007

피틴산(phytic acid)은 inositol hexaphosphate로서 식물성식품 및 씨 중에 1-5%가량 존재하는 자연 항산화 물질로서 소화효소에 의해 쉽게 분해되지 않는다. 한편 인산염은 식육에서 품질개량제로 식육에서의 항균 효과에 대해서는 이미 여러 보고가 있으나 그 결과는 다양하다. 일반적으로 생육에서는 그 효능이 낮은데 이것은 생육에 존재하는 phosphatase의 작용에 의해 인산염들이 가수분해되기 때문이다. 한편 피틴산염은 열에 강하고 쉽게 분해되지 않으므로 생육이나 멸균 처리된 식육에서 공히 인산염들이 가지고 있는 항균효과를 기대할 수 있다. 본 실험에서는 pH가 다른 선택배지에서 Salmonella typhimurium에 대한 피틴산의 항균효과를 평가하였고, 더불어 생육에서 Salmonella typhimurium의 접종 후에 항균 효과를 평가하였다. 선택된 배지인 tryptic soy broth에서 Salmonella typhimurium에 대한 항균 효과는 0.1, 0.5 및 1%의 세 가지 농도에서 농도에 의존적으로 유의성 있게 나타났다. 생육인 닭고기, 돼지고기 및 소고기에 피틴산염을 각각 0.1, 0.5, 그리고 1%의 농도별로 첨가했을 때 Salmonella typhimurium의 증식이 모두 유의성 있게 감소하였다(p<0.01). 또한 피틴산염의 첨가는 모든 식육에서 pH를 0.5-0.6단위만큼 증가시켰다. 이러한 결과로부터 피틴산염은 배지 및 식육에서 항균효과가 뛰어나며 따라서 축산식품의 첨가제로서 사용시 식품의 기능성 향상과 더불어 매우 유용하게 사용될 수 있을 것이다.

• 한국식품위생안전성학회지
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• 제23권3호
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• pp.264-270
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• 2008

대장암은 국민경제 수준의 향상과 식생활의 서구화에 따라 급격하게 증가하여, 등록환자 기준으로 2005년 현재 위암에 이어 두 번째로 많은 상태이다. 특히 육류에 많이 함유된 철분 및 지방의 과다섭취가 주요원인으로 여겨지고 있다. Phytic acid(PA) (Inositol hexaphosphate, IP6)는 식물의 박류, 콩류 등에 약 0.1-5% 농도로 존재하며, 포유류의 세포에서도 존재한다. PA는 금속이온과 결합하는 성질을 갖고 있어서, hydroxyl radical과 같은 활성산소종의 형성을 억제하여 항산화 작용을 나타낸다. 본 연구에서는 PA가 대장암 발생의 전암병변인 aberrant crypt faci(ACF) 의 발생을 억제하는지를 조사하고자 수컷 F344 랫드를 사용하였다. Azoxymethane(AOM)을 실험시작 1주 및 2번째 주에 투여함으로 ACF를 유발하였고, 실험기간은 8주로서 AIN-93G 사료급여와 동시에 음수로서 0.5%와 2% PA를 급여하였다. 부검 후, 혈액검사와 대장암 발생의 초기단계인 ACF를 측정하였다. 결과로서 AOM 투여 대조군과 비교해 볼 때 PA의 농도가 증가함에 따라 ACF와 aberrant crypt(AC)의 수가 감소하였고, 2% PA는 유의적으로 ACF와 AC의 수를 감소시켰으며, 더욱이 4개 이상 AC를 갖는 ACF의 수를 유의적으로 감소시켰다. 또한 혈액 생화학적 수치에서 어느 정도 유의적 변화가 나타났지만 정상범위내에서의 변화로서 인정될 수 있을 것이다. 본 실험에서 이러한 결과는 phytic acid가 대장암 발생과정에 전암병변의 형성을 억제함으로서 최종 대장암발생에 억제효과를 나타낼 수 있을 것이라 사료된다.