• Preventive Nutrition and Food Science
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• v.10 no.2
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• pp.140-144
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• 2005

This study was carried out to establish methods for irradiation detection of irradiation in Korean wheat flour by pulsed photostimulated luminescence (PPSL) and viscometric methods. The photon counts of the irradiated Korean wheat flour measured by PPSL immediately after irradiation increased with increasing irradiation dose. The photon counts in the irradiated Korean wheat flour almost disappeared with lapse of time after storage in normal room conditions, but irradiation detection was still possible after 6 months in darkroom conditions. All irradiated samples indicated a decrease in viscosity with increasing stirring speeds (rpm) and irradiation doses. Irradiation at 1 kGy significantly decreased the viscosity. Consequently, these results suggest that the detection of irradiated Korean wheat powder is possible by both viscometric and PPSL methods.

• Journal of the Korean Society of Food Culture
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• v.15 no.4
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• pp.279-285
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• 2000

A survey was conducted to examine the effect of food irradiation education on college students' knowledge and acceptance of food irradiation. The instrument for the knowledge and acceptance of food irradiation was administered before and after food irradiation education, to 150 students majoring in food and nutrition or food technology in the Chungnam National University. Before the education approximately 93% of the respondents did not know that radioactivity dose not remain in food after irradiation; whereas, after education half of them thought that radioactivity dose not remain in irradiated food. Knowledge about food irradiation has improved through education. The education significantly increased all the mean scores of need for food irradiation and willingness to use irradiated foods for the six food groups (p<0.01). The education significantly decreased the mean scores of concern about the irradiated food for all the six food groups (p<0.01). Although the responses to irradiated foods are, in general, negative or neutral even after education, the mean scores of acceptance of the irradiated foods have improved through education in all the six food groups (p<0.01). In conclusion, this study showed that food irradiation education may positively affect the college students' knowledge and acceptance of food irradiation, and that the development of both the appropriate detection methods to identify irradiated foods and the education programs to enlighten the college students are needed.

• Preventive Nutrition and Food Science
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• v.5 no.3
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• pp.136-141
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• 2000

The properties of pulsed photostimulated luminescence (PPSL) were measured to use as basis data for the detection of irradiated foodstuffs (34 different foods). Samples were packed in polyethylene bags and irradiated at 1, 5, and 10 kGy with a dose rate of 10 kGy/h. The samples irradiated were introduced in the sample chamber without other preparation and measured PPSL photon counts for 60 and 120 s. The PPSL photo counts of the irradiated samples were higher than the unirradiated, increased with increasing irradiation dose, and showed a good relationship between irradiation doses and photon counts in a multinomial expression. These results suggest that the detection of irradiated foodstuffs was possible by PPSL. Therefore, PPSL can be proposed as the method for the detection of irradiated foodstuffs.

• Preventive Nutrition and Food Science
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• v.5 no.3
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• pp.142-147
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• 2000

To establish a detection method of irradiated perilla and sesame seeds, studies were performed with pulsed photostimulated luminescence (PPSL) and thermoluminescence (TL). The PPSL photon counts of the mineral separated from irradiated sesame and perilla seeds were higher than unirradiated one and exhibited an increase with increasing irradiation dose and mineral content. Also TL intensities of minerals separated from irradiated sesame and perilla seeds increased with increasing irradiation dose. In all samples, detection was possible with shapes and maximum TL temperatures of the second glow curves showing lower regions than those of the first glow curves and correctly classified as irradiated samples. Glow curve ratios of irradiated samples were higher than 0.5. These results suggest that PPSL and TL are applicable methods for the detection of irradiated perilla and sesame seeds.

• Preventive Nutrition and Food Science
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• v.7 no.3
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• pp.323-326
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• 2002

Ionizing radiation can be used to sanitize herbs contaminated by various microorganisms. However, health concerns related to irradiation damage to complex molecules in plants necessitate that methods be developed to monitor such damage. To elucidate DNA damage of herbs caused by irradiation, the DNA comet assay was used for Astragalus membranaceus Bunge and Havenia dulcis Thumb, irradiated at 1, 5, 7, and 10 kGy. With increasing irradiation doses, the tails of comets became longer with average tail length increasing from 17 (non-irradiated) to 124 (10 kGy) $\mu$m in Astragalus membranaceus Bunge. Above 7 kGy, some of the tails were separated from the heads of comets. Distribution patterns of the tail length of In comets selected randomly in the irradiated herbs were analyzed to quantify the DNA damage. These results clearly suggest that the DNA comet assay is an effective and inexpensive tool for the detection of irradiation damage to DNA in herbs.

• Journal of the Korean Society of Food Culture
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• v.15 no.4
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• pp.269-277
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• 2000

A survey was conducted to examine the knowledge and acceptance of food irradiation in order to provide baseline data required in the development of food irradiation education programs for college students. 150 students majoring in food and nutrition or food technology in the Chungnam National University were chosen for a survey. The results are as follows. First, college students' knowledge about food irradiation is scanty. Knowledge assessment showed that 56% of the participants had previously heard of food irradiation. 68% of the respondents thought that radioactivity remains in food after irradiation and 25.3% of them were not sure whether radioactivity remains in food after irradiation or not. Only half of the respondents thought that nutrient loss due to irradiation is equal to or lower than that due to cooking or freezing. Second, approximately 56% of the respondents showed that food irradiation is somewhat or strongly needed for meat or fish; whereas, over 60% of them showed that food irradiation is not needed for grain, vegetable and fruit. Almost 40% of the respondents were seriously concerned about irradiation of vegetables and fruits; whereas, they showed less concern about spice irradiation. More than half of the respondents were not willing to use irradiated food in all the six food groups. Third, the correlation analysis showed that the need of food irradiation is negatively correlated with concerning about the irradiated fish and fruits, but positively correlated with willingness to use irradiated food in all the five food groups, except in spices. Concern about the irradiated food is negatively correlated with willingness to use irradiated food from all the six food groups. Fourth, almost all the respondents (over 90%) agreed that the irradiated food labeling is required as well as the development of proper methods to identify irradiated foods.

• Preventive Nutrition and Food Science
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• v.9 no.3
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• pp.222-226
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• 2004

Hydrocarbons induced by gamma-irradiation of green, black, and oolong teas were analyzed to determine whether the hydrocarbons can be used as markers for detecting post-irradiation of these teas. The samples were irradiated at 0, 2.5, 5, 7.5, and 10 kGy. Detection was attempted by extracting fat from the teas, separation of hydrocarbons with florisil column chromatography, and identification of hydrocarbons by gas chromatography-mass spectroscopy (GC-MS). Concentration of hydrocarbons increased with the irradiation dose. The major hydrocarbons in irradiated green, black, and oolong teas were 1-tetradecence (14:1), pentadecane (15:0), 1,7-hexadecadiene (16:2), 1-hexadecene (16:1), 8-heptadecene (17:1), and heptadecane (17:0). Radiation-induced hydrocarbons in teas were 1,7-hexadecadiene and 8-heptadecene. These compounds were not detected in non-irradiated samples, so the hydrocarbons (16:2, 17:1) can be used as markers for detecting post-irradiation of the teas. Furthermore, detection of hydrocarbons after 12 months storage at room temperature remains a suitable method for identifying irradiated teas.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.1
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• pp.63-68
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• 1998

Irradiation can be utilized to inhibit sprouting of onions and garlic. Thermoluminescence(TL) is a potential method to detect post-irradiation of onions and garlic because they are irradiated with soil or dusts on them, from which minerals can be isolated for TL analysis. This study was to determine whether TL can be applied for the detection of post-irradiation of onions and garlic. Onions and garlic produced in Korea were irradiated in the range of 0.01~0.6kGy. Minerals isolated from the samples using sodium polytrungstate solution were used for TL analysis. The onions and garlic irradiated at 0.15kGy or higher could be detected with little errors. Minimum dose for the 2nd glow could be applied since the dose for the 2nd glow did not affect the results. The TL intensities of the temperature ranges of 229~295$^{\circ}C$ and 229~361$^{\circ}C$ and the maximum intensity provided more accurate information for the determination of the post-irradiation of onions and garlic.

• Preventive Nutrition and Food Science
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• v.10 no.1
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• pp.29-33
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• 2005

To facilitate establishing regulations for irradiated foods, Korean traditional soybean-based fermented powdered doenjang (PD), kanjang (PK), kochujang (PKC) and chungkukjang (PC) were irradiated at 1, 3, 5 and 7 kGy, and subjected to irradiation detection analyses as part of establishing a database for detecting irradiated foods. Photostimulated luminescence (PSL) and electron spin resonance (ESR) were applied as the detection methods. Using PSL analysis, the irradiated PD, PK and PKC could be easily distinguished from the non-irradiated ones, while irradiation of the PC at 5 kGy or higher was detectable. The ESR spectra of the irradiated PD, PK and PKC exhibited symmetrical multiplet lines, which might be induced from the crystalline sugar, whereas, the PC showed a single signal at the paramagnetic centers. The signal intensity increased with incremental increases of irradiation doses distinguishing the irradiated samples from the control. In addition, the peak height also revealed that irradiation induced an increment in the intensity of single and/or multiplet lines of the ESR signals, resulting in clear confirmation of irradiation. Thus, the data from this study could be used as references for detecting irradiated soybean-based fermented powdered sauces.

• Journal of Food Hygiene and Safety
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• v.16 no.1
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• pp.82-87
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• 2001

A study was performed to establish detection methods by viscometric and pulsed photostimulated luminescence (PPSL) methods for irradiated com powder. Viscosity was determined using a Brookfield DV-rotation viscometer at 3$0^{\circ}C$ and operated at 30, 60, 90, 120, 150, 180, and 210 rpm. All irradiated samples showed a decrease in Viscosity with increasing stirring speeds (rpm) and irradiation doses. Treatments at 1~3 kGy significantly decreased the viscosity. The photon counts of irradiated corn powder were measured by PPSL immediately after irradiation and exhibited an increase with increasing irradiation dose. The photon counts of irradiated com powder almost disappeared with lapse of time in room conditions, but detection of irradiation was still possible after one month at darkroom conditions. Consequently, these results suggest that the detection of irradiated com powder is possible by both viscometric and PPSL methods.