• Title/Summary/Keyword: jejunal crypt

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Radiosensitivity and Dobe-Survival Characteristics of Crypt Cells of Mouse Jejunum (마우스공장 소낭선세포의 방사선 감수성에 관한 실험적 연구)

  • Huh, Seung-Jae;Park, Charn-Il
    • Radiation Oncology Journal
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    • v.3 no.1
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    • pp.9-12
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    • 1985
  • To determine the radiosensitivity and dose-survival characteristics of jejunal crypt cells, experimental study was done using total 40 mice. Single irradiation of 1,000 rad to 1,600rad was delivered to whole bodies of mice, using a cesium 137 animal irradiator. The number of regenerating crypts per jejunal circumference was counted, by using a jejunal crypt cell assay technique, and dose response curve was measured. The average number of jejunal crypt Per circumference in control group was $140\pm10$. Mean lethal dose$(D_0)$ of moose jejunal crypt cell was 135rad.

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Radiation Effect on Mouse Jejunal Crypt Cells by Single and Split Irradiation (단일조사와 분할조사시 마우스 공장 소낭선세포의 방사선효과에 관한 실험적 연구)

  • Koh Byung Hee;Hahm Chang Kok;Kim Jung Jin;Park Chan Il
    • Radiation Oncology Journal
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    • v.3 no.1
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    • pp.1-8
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    • 1985
  • To determine the dose·survival and repair characteristics of the jejunal crypt cells, experimental study was carried out using total 70 mice. Single or split irradiations of 1,100 to 2,200 rad were delivered to whole bodies of $C_{57}$ BL mice, using a cesium 137 animal irradiator and those mice were sacrificed after 90 hours. The number of regenerating crypts per jejunal circumference was counted by a jejunal crypt cell assay technique and dose·response curve was measured. The results were as follows : 1. The average number of jejunal crypts per circumference in control group was 140. In a single irradiation group, the number of regenerated jejunal crypts was, 125, 56, 2 in each subgroup of 1,100 rad, 1,400 rad and 1,800 rad respectively. In split irraiation group, it was 105,44,2 in each subgroup of 1,400rad 1,800rad and 2,200rad respectively. 2. Mean lethal dose of mouse jejunal crypt cell was 167 and 169 rad respectively in a single and split irradiation. 3. Repair dose of sublethal damage was 280 rad. 4. Sublethal damage was completely repaired within 4 hours between the split dose of irradiation.

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The Effects of Shi-Quan-Dai-Bu-Tang and Its Ingredients on the Survival of Jejunal Crypt Cells and Hematopoietic Cells in Irradiated Mice (방사선 피폭 마우스에서 소장움세포 및 조혈세포 생존에 미치는 십전대보탕 및 구성단미의 영향)

  • Jo, Sung-Kee;Yu, Young-Bub;Oh, Heon;Kim, Se-Ra;Kim, Sung-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.1
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    • pp.93-98
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    • 2000
  • We performed this study to determine the radioprotective effects of Shi-Quan-Dai-Bu-Tang, as a prescription of traditional Oriental medicine, and its major ingredients. The jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells were investigated in mice irradiated with high and low dose of ${\gamma}$-rays. The administration of danggui, baishaoyao, rensan, gancao or baifuling before irradiation protected the jejunal crypts (p<0.005). Shoudehuang, danggui, baishaoyao, rensan and huangqui increased the formation of endogenous spleen colony (p<0.05). Chuanxiong, baishaoyao, rensan and baifuling reduced the frequency of radiation-induced apotosis (p<0.05). The results indicated that the extracts of danggui, baishaoyao, rensan and baifuling may have radoprotective effects in mice irradiated with high and low dose of ${\gamma}$-rays. The radioprotective effect of the prescription, Shi-Qaun-Da-Bu-Tang, was not significant.

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Effect of Red Ginseng, White Ginseng and Diethyldithoicarbamate in Irradiated Mice (방사선 피폭 마우스에서 홍삼, 백삼 및 diethyldithoicarbamate의 효과)

  • 김성호;이해준;김세라;이종환;조성기;나승렬;손창호;신동호
    • Journal of Ginseng Research
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    • v.25 no.2
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    • pp.68-73
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    • 2001
  • Studies were performed to determine the effect of red ginseng and white ginseng on jejunal crypt survival, endogenous spleen colony formation, and apoptosis of jejunal crypt cells in irradiated mice. The radioprotective effect of ginseng was compared with the effect of diethyldithocarbamate(D). Jejunal crypts were protected from irradiation by pretreatment of red ginseng (50 mg/kg B.W., I.P. at 36 and 12 hours before irradiation) and white ginseng (50 mg/kg B.W., I.P. at 36 and 12 hours before irradiation). Red ginseng administration before irradiation and both pretreatment and posttreatment (50 mg/kg B.W., I.P. at 30 minutes after irradiation) of white ginseng resulted in an increase of the formation of endogenous spleen colony. the frequency of radiation-induced apoptosis in intestinal crypt cells was also reduced by both pretreatment and posttreatment of red ginseng, and pretreatment of white ginseng. The radioprotective effect of DDC (1000 mg/kg B.W., I.P. at 30 minutes before irradiation) on jejunal crypt survival and apoptosis was similar to those of ginseng treatment. Treatment with DDC showed no significant modifying effects on formation of endogenous spleen colony. These results indicated that ginseng might be a useful radioprotector. Further studies are needed to characterize effective radioprotective components and mechanism of ginseng.

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Immunostimulating Effects of Angelica Gigas by Radio-Protective (당귀의 방사선 방호에 의한 생체면역 증진 효과)

  • Kim Kyung-Yoon;Jeong Hyun-Woo;Kim Gye-Yeop
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.5
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    • pp.1256-1260
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    • 2005
  • The purpose of this study was to investigate the effects of Angelica gigas on jejunal survival, endogenous spleen colony formation and jejunal crypt cells of mice irradiated with Gamma-ray irradiation. The subject of this study includes 42 mice which were divided into each 7 groups. Angelica gigas experiment groups were Angelica gigas + Gamma-ray(10Gy), Angelica gigas + Gamma-ray(3Gy), Angelica gigas. Gamma-ray(1 Gy), Gamma-ray control (10Gy), Gamma-ray control(3Gy), Gamma-ray control(1Gy), Normal groups. In the present study to evaluate the effect of Angelica gigas on jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells of mice Gamma-ray with each dose of Gamma-ray irradiation. The results of this study were as follows: In low-dose(1Gy) Gamma-ray radiation were treatment of Angelica gigas showed significantly increased(p<0.05) on the cell death apoptosis in crypt, intestine crypts survival of intestine after gamma-ray irradiation. High-dose(10Gy) Gamma-ray, treatment of Angelica gigas showed significantly increased(p<0.05) on the leukocyte. The above results suggest that Angelica gigas were immunostimulating effectively reduced Gamma-ray irradiation.

Effects of Sun Ginseng on the Crypt Cell Survival in Mice Irradiated with High-energy X-ray (선삼 투여가 고에너지 X-선을 조사한 마우스에서 소장움 재생에 미치는 효과)

  • Sin, Jung-Sub;Park, Jeong-Hill;Kim, Sung-Ho;Kim, Hun-Jeong;Kim, Young-Chul
    • Environmental Analysis Health and Toxicology
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    • v.22 no.1 s.56
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    • pp.73-82
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    • 2007
  • Six week-old ICR mite which were divided into four groups including NC, RC, RR and RS were injected with sun ginseng (RS), red ginseng (RR) and saline (RC) intraperitonedlly as an amount of 60 mg/g body weight at 1 hour, 12 hours and 36 hours before the irradiation of high-energy X-ray and the mire were sacrificed at three and a half days after the irradiation. The RS group were significant increase in the weight of spleen (p<0.01) and the numbers of jejunal crypt cells (P<0.01), WBC (p<0.05), lymphocytes (p<0.05) and neutrophils (p<0.05) in comparison with the RC group. The RR group were significant increase in the numbers of jejunal crypt cells (p<0.001), WBC (p<0.05) and neutrophils (p<0.05) in comparison with the RC group. The RS group exhibited a more increase in the weights of spleen and thymus and the numbers of jejunal crypt cells and all items of hematological examination than the RR group. The values of ALT (alanine transaminase) and AST (aspartate transaminase) were significantly elevated (p<0.05) by radiation and they were significantly decreased (p<0.05) in the RS group to the values of the NC group. Taken together the above results, sun ginseng demonstrated a jejunal crypt survival effect, the protective effects on hepatocytes and immune and hematopoietic cells in mice irradiated with high-energy X-ray, and those radiation protective effects were a little higher in comparison with red ginseng.

Morphological evaluation on the effect of panaxadiol series ginsenosides in irradiated mice (방사선 조사 마우스에서 인삼 panaxadiol계 ginsenosides의 효과에 관한 형태학적 평가)

  • Lee, Hae-june;Kim, Se-ra;Kim, Sung-ho
    • Korean Journal of Veterinary Research
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    • v.44 no.2
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    • pp.179-184
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    • 2004
  • The purpose of the study was to investigate the effect of ginseng saponins (panaxadiol, ginsenoside $Rb_1$, $Rb_2$, Rc, Rd) on jejunal crypt survival, endogenous spleen colony formation and apoptosis in jejunal crypt cells of mice irradiated with gamma-ray. ICR mice were given each saponin (i.p. 50 mg/kg of body weight) at 24 hours before irradiation. The radioprotective effects of saponins were compared with the irradiation control respectively. The jejunal crypts were protected by pretreatment with ginsenoside Rc (p<0.05) and Rd (p<0.05). The spleen colony was increased by pretreatment with panaxadiol (p<0.05) and ginsenoside Rd (p<0.05). And the frequency of radiation induced apoptosis was significantly reduced by pretreatment with panaxadiol (p<0.05), ginsenoside Rb2 (p<0.05), Rc (p<0.05) and Rd (p<0.01). These results suggest that ginsenoside Rc, Rd might have a major radioprotective effect.

The radioprotective effects of green tea and its fractions in Gamma-irradiated mice (감마선 조사 마우스에서 녹차 및 분획의 방사선 장해 경감 효과)

  • Kim, Se-ra;Lee, Hae-june;Kim, Sung-ho
    • Korean Journal of Veterinary Research
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    • v.43 no.4
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    • pp.633-639
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    • 2003
  • We investigated the effect of green tea and its fractions of alcohol and polysaccharide on jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells of mice irradiated with high and low dose of gamma-irradiation. Jejunal crypts were protected by pretreatment of green tea (i.p.: 50 mg/kg of body weight, at 12 and 36 hours before irradiation., p.o.: 1.25% water extract, for 7days before irradiation, p<0.01) and alcohol and polysaccharide fractions showed no significant modifying effects. Green tea and its fractions administration before irradiation (i.p. at 12 and 36hours before irradiation) resulted in an increase of the formation of endogenous spleen colony (p<0.05). The frequency of radiation-induced apoptosis in intestinal crypt cells was also reduced by pretreatment of green tea (i.p. at 12 and 36 hours before irradiation, p<0.05., p.o. for 7days before irradiation, p<0.001) and its fractions (p<0.001). These results indicated that green tea might be a useful radioprotector, especially since it is a relatively nontoxic natural product. Further studies are needed to characterize better the promotion nature of green tea and its components.

Protective Effects of Trithioformaldehyde against Radiation Damage of Mouse Jejunal Crypt Cells (TTFA의 마우스 공장 소낭선에 대한 방사선 방호작용)

  • Lee, Jong-Hwoa;Kwon, Jun-Tack;Cho, Byung-Heon
    • The Korean Journal of Pharmacology
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    • v.24 no.2
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    • pp.217-220
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    • 1988
  • At present, the treatment of the radiation-induced diseases are only performing by the palliative treatment technique. Moreover, radioprotective drugs are a little toxic for human being and this seriously limits their application with various complication in clinical uses. Accordingly, new radioprotectors need developing. In our Lab., we synthesized trithioformaldehyde (TTFA), containing three sulfur atom, and examined the effect on mouse jejunal crypt cells after irradiation. Mice treated with TTF A (2.0 g/ kg) showed 78% survival ratio at 30 day after 800 rad irradiation. 1.0 g/kg and 2.0 g/kg of TTF A increased resistance of jejunal crypt cells to single doses of radiation by protection factors of 1.17 and 1.23, respectively.

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Immunostimulating Effects of Acanthopanax in mice following Gamma-ray irradiation (방사선 조사 마우스에서 오가피의 면역활성 효과)

  • Kim, Gye-Yeop;Jeong, Hyun-Woo;Kim, Kyung-Yoon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.20 no.3
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    • pp.670-674
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    • 2006
  • This experimental study was carried out to investigate the immunostimulating effect of Acanthopanax, as Oriental rhizomata herbs, on jejunal survival, endogenous spleen colony formation, apoptosis in jejunal crypt cells and lipid peroxidation in the liver of mice following Gamma-ray irradiation. The subject of this study includes 72 mice which were divided into each 7 groups. Acanthopanax experiment groups were Acanthopanax. Gamma-ray(lOGy), Acanthopanax. Gamma-ray(3Gy), Acanthopnax. Gamma-ray(1Gy), Gamma-ray control(1OGy), Gamma-ray control(3Gy), Gamma-ray control(1Gy), Normal groups. The results of this study were as follows : Treatment with Acanthopanax showed significantly increased(p<0.05) on the cell death apoptosis in crypt, intestine crypts survival of intestine in mice following low-dose(1Gy) Gamma-ray radiation. And that significantly increased(p<0.05) on jejunal crypt survival and reduced(p<0.05) on lipid peroxidation in mice following high-dose(1OGy) Gamma-ray radiation. The above results suggest that Acathopanax were immunostimulating effectively reduced Gamma-ray irradiation.