• 한국균학회소식:학술대회논문집
• /
• 2009.10a
• /
• pp.61-69
• /
• 2009

• The Plant Pathology Journal
• /
• v.35 no.1
• /
• pp.11-18
• /
• 2019

Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. $TUB{\beta}$ was identified as the most stable reference gene. Furthermore, the exopolygalacturonase gene (ExoPG) was selected to verify the reliability of $TUB{\beta}$ expression. The expression profile of ExoPG assessed using $TUB{\beta}$ agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.

• The Plant Pathology Journal
• /
• v.37 no.5
• /
• pp.437-445
• /
• 2021

Tilletia laevis Kuhn (syn. Tilletia foetida (Wallr.) Liro.) causes wheat common bunt, which is one of the most devastating plant diseases in the world. Common bunt can result in a reduction of 80% or even a total loss of wheat production. In this study, the characteristics of T. laevis infection in compatible wheat plants were defined based on the combination of scanning electron microscopy, transmission electron microscopy and laser scanning confocal microscopy. We found T. laevis could lead to the abnormal growth of wheat tissues and cells, such as leakage of chloroplasts, deformities, disordered arrangements of mesophyll cells and also thickening of the cell wall of mesophyll cells in leaf tissue. What's more, T. laevis teliospores were found in the roots, stems, flag leaves, and glumes of infected wheat plants instead of just in the ovaries, as previously reported. The abnormal characteristics caused by T. laevis may be used for early detection of this pathogen instead of molecular markers in addition to providing theoretical insights into T. laevis and wheat interactions for breeding of common bunt resistance.

• Nuclear Engineering and Technology
• /
• v.54 no.1
• /
• pp.269-274
• /
• 2022

An artificial neural network (ANN) that identifies radionuclides from low-count gamma spectra of a NaI scintillator is proposed. The ANN was trained and tested using simulated spectra. 14 target nuclides were considered corresponding to the requisite radionuclide library of a radionuclide identification device mentioned in IEC 62327-2017. The network shows an average identification accuracy of 98.63% on the validation dataset, with the gross counts in each spectrum N_c = 100~10000 and the signal to noise ratio SNR = 0.05-1. Most of the false predictions come from nuclides with low branching ratio and/or similar decay energies. If the N_c>1000 and SNR>0.3, which is defined as the minimum identifiable condition, the averaged identification accuracy is 99.87%. Even when the source and the detector are covered with lead bricks and the response function of the detector thus varies, the ANN which was trained using non-shielding spectra still shows high accuracy as long as the minimum identifiable condition is satisfied. Among all the considered nuclides, only the identification accuracy of ²³⁵U is seriously affected by the shielding. Identification of other nuclides shows high accuracy even the shielding condition is changed, which indicates that the ANN has good generalization performance.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.19
• /
• pp.8051-8055
• /
• 2014

Background: A common genetic variant rs3757318, located in intron of C6orf97, was firstly identified to be associated with breast cancer (BC) risk by a genome-wide association (GWA) study. However, subsequent validation studies with different ethnicities have yielded conflicting results. Materials and Methods: We performed a meta-analysis to synthesize all available data for evaluating the precise effect of this variant on BC susceptibility. Results: A total of 8 articles containing 11 studies with 62,891 cases and 65,635 controls were included in this meta-analysis. When compared to the G allele, the rs3757318-A allele was significantly associated with BC risk with the pooled OR of 1.21 (95% CI=1.15 - 1.29, P<0.001) but with obvious between-study heterogeneity (P=0.040). Stratified analysis suggested that diversity of ethnicity along with control source may explain part of the heterogeneity. Similarly, significant associations were also identified in heterozygote, homozygote, dominant and recessive genetic models. Sensitivity and publication bias analyses indicated robust stability of our results. Conclusions: Our present meta-analysis demonstrated that the variant rs3757318 is associated with increased BC risk. Nevertheless, further studies are needed to clarify the underlying biological mechanisms.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.15 no.7
• /
• pp.545-552
• /
• 1990

This paper is concerned with the file protection in the file transfer systems. In the existing file transfer systems, passwords are used in the protection but do not provide any data protection and can only provide some protection against unauthorized access. Even provided with this protection, we cannot be free form computer hackers. In order to achieve higher standards of protection for our privacy (protection for data themselves, authentication of senders...) analternative technical system should be developed in using of pulic key cryptography by choosing the public key method (RSA public key) in the file transfer. A new system suggested in the paper can achieve some higher standards of protection for our privacy. We a result thie system will be easily applied to various document handling systems as in the data base.

• ETRI Journal
• /
• v.28 no.1
• /
• pp.99-102
• /
• 2006

In this letter, we study the protection problem in wavelength division multiplexing (WDM) optical networks, and propose a novel dynamic heuristic algorithm called differentiated reliable segment protection (DRSP). Differing from previous work, DRSP can effectively avoid the trap problem and is able to find a feasible solution for each connection request. Therefore, DRSP outperforms the previous work. Simulation results have shown to be promising.

• Journal of Microbiology and Biotechnology
• /
• v.19 no.10
• /
• pp.1250-1258
• /
• 2009

Bacillus spp., as a type of plant growth-promoting rhizobacteria (PGPR), were studied with regards promoting plant growth and inducing plant systemic resistance. The results of greenhouse experiments with tobacco plants demonstrated that treatment with the Bacillus spp. significantly enhanced the plant height and fresh weight, while clearly lowering the disease severity rating of the tobacco mosaic virus (TMV) at 28 days post-inoculation (dpi). The TMV accumulation in the young non-inoculated leaves was remarkably lower for all the plants treated with the Bacillus spp. An RT-PCR analysis of the signaling regulatory genes Coil and NPR1, and defense genes PR-1a and PR-1b, in the tobacco treated with the Bacillus spp. revealed an association with enhancing the systemic resistance of tobacco to TMV. A further analysis of two expansin genes that regulate plant cell growth, NtEXP2 and NtEXP6, also verified a concomitant growth promotion in the roots and leaves of the tobacco responding to the Bacillus spp.

• The Plant Pathology Journal
• /
• v.35 no.3
• /
• pp.208-218
• /
• 2019

Here, we reported a novel secreted protein elicitor PeBL2 from Brevibacillus laterosporus A60, which can induce hypersensitive response in tobacco (Nicotiana benthamiana). The ion-exchange chromatography, high-performance liquid chromatography (HPLC) and mass spectrometry were performed for identification of protein elicitor. The 471 bp PeBL2 gene produces a 17.22 kDa protein with 156 amino acids containing an 84-residue signal peptide. Consistent with endogenous protein, the recombinant protein expressed in Escherichia coli induced the typical hypersensitive response (HR) and necrosis in tobacco leaves. Additionally, PeBL2 also triggered early defensive response of generation of reactive oxygen species ($H_2O_2$ and $O_2{^-}$) and systemic resistance against of B. cinerea. Our findings shed new light on a novel strategy for biocontrol using B. laterosporus A60.

• International journal of steel structures
• /
• v.18 no.4
• /
• pp.1153-1166
• /
• 2018

This paper was concerned with the nonlinear analysis on the overall stability of H-type honeycombed composite column with rectangular concrete-filled steel tube flanges (STHCC). The nonlinear analysis was performed using ABAQUS, a commercially available finite element (FE) program. Nonlinear buckling analysis was carried out by inducing the first buckling mode shape of the hinged column to the model as the initial imperfection with imperfection amplitude value of L/1000 and importing the simplified constitutive model of steel and nonlinear constitutive model of concrete considering hoop effect. Close agreement was shown between the experimental results of 17 concrete-filled steel tube (CFST) specimens and 4 I-beams with top flanges of rectangular concrete-filled steel tube (CFSFB) specimens conducted by former researchers and the predicted results, verifying the correctness of the method of FE analysis. Then, the FE models of 30 STHCC columns were established to investigate the influences of the concrete strength grade, the nominal slenderness ratio, the hoop coefficient and the flange width on the nonlinear stability capacity of SHTCC column. It was found that the hoop coefficient and the nominal slenderness ratio affected the nonlinear stability capacity more significantly. Based on the results of parameter analysis, a formula was proposed to predict the nonlinear stability capacity of STHCC column which laid the foundation of the application of STHCC column in practical engineering.