• Journal of Veterinary Science
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• 제24권2호
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• pp.33.1-33.7
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• 2023

Background: Laminitis is a complex and debilitating disease of horses. Numerous predisposing factors contribute to laminitis development, however the exact pathogenesis remains undetermined. Serum T4, cortisol, and histamine are components of the innate stress response and could play a causative or contributory role. Stress hormone concentrations in laminitis are largely unknown. Objective: To evaluate parameters associated with stress response in horses with laminitis, and compare these to healthy horses and horses with gastrointestinal (GI) disease. Methods: Thirty-eight adult horses presenting for non-medical conditions, GI abnormalities, or clinical laminitis were prospectively enrolled. Horses were assigned to the appropriate disease group (healthy, GI disease, and laminitis) and had blood drawn on presentation to the hospital. Samples were analyzed for plasma endogenous adrenocorticotrophic hormone (eACTH), serum cortisol, serum thyroid hormone, and plasma histamine. Results: Stress hormone concentrations were significantly different between horses in the laminitis and GI disease groups. Plasma histamine levels were highest in horses with laminitis, compared with GI disease and controls. Both horses with laminitis and GI disease had increased plasma eACTH when compared to healthy horses. Horses with GI disease had higher serum cortisol concentrations than horses with laminitis or controls. Serum T4 was lower in horses with GI disease than in horses with laminitis and controls. Conclusions: Horses with laminitis had relative increases in both plasma histamine and eACTH concentrations. Serum T4 and cortisol concentrations of horses with laminitis did not differ significantly when compared to healthy horses. The role of stress hormones in equine disease warrants further investigation.

• 한국임상수의학회지
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• 제32권1호
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• pp.62-68
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• 2015

There is no reliable indicator available for the diagnosis of horse laminitis, although the disease is common and costly. This study was performed to develop a specific diagnostic biomarker for laminitis. We have identified 33 differentially expressed proteins in plasma of a horse suffering laminitis that is experimentally induced by an overdose of oligofructose, in comparison with normal horse plasma. Among the proteins, myosin-9 mRNA was found in RNA sequencing analysis to be expressed specifically in laminitis tissues compared to other horse tissues. It is thus suggested that expression of plasma myosin-9 may be used for the diagnosis of equine laminitis.

• 한국임상수의학회지
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• 제30권1호
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• pp.75-79
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• 2013

Laminitis is a common horse disease that causes pain and disability. One of the major causes of laminitis is carbohydrate overload, which leads to the overgrowth of gram-positive bacteria and subsequent toxemia. This study was performed to establish an experimental model of acute phase laminitis. A horse was fed with a bolus dose of oligofructose, examined for clinical signs and blood parameters, and euthanized for necropsy and histopathology. After administration, the horse exhibited clinical signs including watery feces, inappetence, severe lameness, pyrexia and tachycardia. Complete blood count showed increased hematocrit, lymphocytosis and neutropenia. Serum chemistry revealed hyperglycemia, hyperproteinemia, high level of glutamic oxalate transaminase, creatinine, uric acid and mild hyponatremia. Histologically, the lamina tissue was edematous with mild infiltration of inflammatory cells. These findings indicate that oligofructose-induced laminitis in horse is an appropriate model for studying pathogenesis of acute phase laminitis.

• 한국임상수의학회지
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• 제38권1호
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• pp.41-44
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• 2021

Pituitary pars intermedia dysfunction (PPID), often referred to as equine Cushing's disease, is a common endocrine disorder often diagnosed in older horses. A 13-year-old 460 kg Warmblood gelding showed clinical signs suggestive of PPID, including hypertrichosis, fat redistribution, polyuria and polydipsia (PU/PD), and weight loss. Physical examination, complete blood cell count, and serum chemistry results were normal. However, dexamethasone suppression and plasma adrenocorticotropic hormone (ACTH) level tests confirmed PPID. Three months after the confirmed diagnosis, the horse was referred again with symptoms of laminitis. Radiography and venography were performed to evaluate the laminitis severity level. However, the foot condition continued to worsen, and the horse was eventually euthanized. The purpose of this case report is to describe clinical signs and diagnosis of PPID with laminitis.

• 한국임상수의학회지
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• 제23권1호
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• pp.6-8
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• 2006

아일랜드 갈웨이주에서 사육중인 13살, 수컷, 아랍종말이 제엽염으로 진단되었다. TH01, SI01 및 ST45에 혈침 및 덱사메타손 수침(2회/주)을 각각 실시하였다. 또한 2회째 봉독(아피톡신 $200{\mu}g$을 동량의 리도카인에 혼합)을 GV01에 수침하였다. 3회 침치료 후 말은 정상 보행이 가능하였다.

• 한국임상수의학회지
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• 제15권1호
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• pp.62-74
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• 1998

In feedlot cattle the abrupt change of diet from roughage to a large quantity of grain for the purpose to improve production often results in increased occurrence of rumen acidosis or acute carbohydrate encouragement enterotoxemia, bloats diarrhea liver abscess and laminitis or robot disease. The common management practice to control these problem is to increase the amount of concentrates in the diet in a stepwise manner until the animals are adapted to a high-grain ration. However this practice requires at least about 3 weeks adaptation period and specially prepared adaptation rations which contain various amount of concentrates. Present experiment was undertaken in order to findout the more simple and rapid adaptation method of cattle to a high grain ration. Nineteen Korean calves aging from four to six month were fed artifical hay (Youngchoun Chuk-Hyup, Korea) which contains 10% of concentrates or alfalfa and rye grass hays for two months and randomly alloted to three experimental groups and two control groups. The experimental group-1 was inoculated by stomach tube for two days with li500 ml/day of ruminal fluid fished from Korean beef cattle that had been previously adapted to a high-energy ration. The experimental group-2 was inoculated by trocalization for two days with the same ruminal fluid. The experimental group-3 was inoculated by trocalization with 1,500 ml/day of bacterial culture which contained 2$\times $10$^{9}$/m1 of Gram-negative bacteria derived from adapted luminal fluid. The two control groups were treated with normal saline solution by the same methods. All animals were fed high-energy ration that contained 80% of grain ad libitum for 30-74 days beginning on the third of the treatment. The effect of the inoculation on the adaptation was observed clinicopathologically with the following results; All of the experimental calves inoculated with the ruminal fluid or Gram-negative bacterial culture derived from adapted cattle did not show any signs of rumen acidosis or other related diseases, while most of the control calves did show diarrhea and bloat and a calf laminitis. The average daily weight gain and feed efficiency of experimental calves were slightly improved compared with control calves. Following the feeding of high-grain rational the pH of the ruminal fluid was lowered in both the experimental and control groups. However severe acidosis with the pH of below 5.0 was observed in only a control group-2. The protozoal number in ruminal fluid was markedly decreased during the high-grain feeding in both the experimental and control calves. However the decrease was mere severe in control calves compared with the experimental calves. The activation of the protozoa were completely disappeared within nine hours at the refrigerator temperature (4"C). No significant differences in heamatological and blood chemical values between the experimental and control calves were recognized. However in one control calf which showed clinically laminitis marked elevations of serum glutamic oxaloacetate transaminase and lactic dehydrogenase activities and a decrease of serum glucose level were observed. From these results it would be concluded the intraruminal transplantation of unadapted calves with the adapted ruminal fluid from cattle previously adapted to a high-energy ration prevents disease problem associated with high-grain feeding and improve weight gain and feed efficiency.ency.

• 한국동물위생학회지
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• 제24권4호
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• pp.347-352
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• 2001

This study was carried out to investigate the status of hoof diseases and to develope treatment method for cattle with hoof diseases during the period of January to December in 2000. Out of 435 heads, 34 heads(7.8%) had hoof diseases. In respect to season, incidence rate was higher in August to September than that of other seasons. The incidence rates of hoof rot, pododermatitis verrucosae, laminitis, other cases and trauma were 14 cases(41％), 10 cases (29%), 7 cases(21%), 2 cases(6%) and 1 case(3％), respectively. In respect to age and milk production, Incidence rate was higher in the cattle with high milk production. Incidence rate of hoof diseases on the hind-limbs was higher than that of the fore-limbs. E. coli(8 strains), Clostridium spp(8 strains), Staphylococcus spp(6 strains), Fusobacterium spp(6 strains), and Bacteroides spp(6 strains) were isolated from the hoof lesions. All isolates were sensitive to composite preparation made of mainly formalin with picric acid and phenol. (Treatment against each strains isolated was sensitive to composite preparation made out of the main constitutions of formalin with picric acid and phenol).

• Journal of Animal Science and Technology
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• 제52권2호
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• pp.111-116
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• 2010

본 연구는 말의 다양한 사양체계가 분 젖산 생산 박테리아 및 pH 변화에 미치는 영향을 구명하기 위해 수행되었다. 시험 1에서는 농후사료 체중 2% 또는 건초(오챠드그라스) 2%를 30일 동안 급여한 후 분 중 Lactobacillus와 Streptococcus 균 수를 측정했다. 시험 2에서는 사양형태 또는 사료급여 수준에 따른 분 중 pH 변화를 측정했다. 시험 3에서는 알팔파 건초 급여 수준에 의한 분 pH 변화를 측정했다. 고－농후사료의 급여는 분 중 젖산 생산 박테리아인 lactobacillus와 streptococcus의 균 수를 크게 증가시켰다(P<0.05). 농후사료의 급여량을 증가시킴에 따라 분 pH는 유의적으로 감소했다(P<0.01). 알팔파 건초 단독 급여에서는 분 pH가 저하되지 않았으나, 알팔파 건초+농후사료 사양형태에서는 분 pH가 감소되었다(P<0.01). 본 연구 결과, 말에게 농후사료의 다량급여는 장내 젖산생산 박테리아의 활성을 증가시키며, 농후사료 급여량이 증가함에 따라 분 pH를 저하시키고 있음을 알 수 있다. 따라서 말에게 적절한 조사료/농후사료 비율을 유지하고 급여량을 조절하는 것이 필요하다. 또한 분 pH 측정으로 인해 대장 내 상태를 가늠할 수 있고, 분 pH 값을 이용해 사양관리의 지표로 활용할 수 있을 것으로 사료된다.

• 한국임상수의학회지
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• 제31권2호
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• pp.102-107
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• 2014

본 연구는 한국마사회 동물병원에서 시행한 전신마취 결과를 분석하고 마취 중 후에 발생한 사망률에 영향을 미치는 인자를 찾기 위하여 실시되었다. 총585두의 환마에서 전신마취가 실시되었으며, 수술의 빈도는 정형외과 수술(410)이 가장 많이 실시되었고, 산통 수술(85), 호흡기 수술(45) 순이었다. 585두 중 20두가 마취 중 후에 죽거나 안락사 되었다. 20두 중 14두는 산통 수술을 받았고, 3두는 정형외과 수술, 3두는 호흡기 수술을 받았다. 세부 사망원인으로는 산통 수술에서는 장파열, 호흡기 수술에서는 기도폐색이었다. 정형외과 수술에서는 근육병증, 재골절, 제엽염이 안락사의 원인이었다. 결과적으로 산통 수술과 호흡기 수술 후의 사망률은 정형외과 수술 후보다 높게 나타났다 (p < 0.01). 이상의 결과로부터, 산통수술을 받는 말의 사망률이 가장 높은 것을 확인하였으며, 그 원인은 예후불량에 의한 안락사이었다. 말의 마취 전후 사망률을 낮추기 위하여, 산통 수술을 받는 말은 수술 전후의 집중 관리가 필요하고, 호흡기 수술을 받는 말은 회복 중 주의 깊은 관찰이 추천된다.

• 한국초지조사료학회지
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• 제33권4호
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• pp.313-318
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• 2013

본 시험은 젖소 4두에 대하여 제1위체류형 센서(Indwelling probe)를 정치시키고 이를 이용하여 아급성제1위과산증(SARA)의 강도를 조사함과 동시에 pH가 혈 중 내독소(Lipopolysaccharide; LPS)의 농도에 미치는 효과를 알아보기 위하여 수행되었다. 비섬유성탄수화물(Non-fiber carbohydrates, NFC)을 기준으로 적정 수준의 NFC를 포함하고 있는 정상 사료군(Normal diet, ND)과, 아급성제1위과산증(Subacute ruminal acidosis, SARA) 유발가능 사료군(Acidogenic diet, AD)의 2개 처리구에 각 2두씩 배치하여 Cross-over 설계로 사양실험을 반복 실시하였다. 제1위체류형 센서는 장시간에 걸친 제1위 내 pH의 변화를 추적하는 데 효과적이었다. 얻어진 결과에서 AD 급여는 반추위내용물의 pH를 낮추는 데 커다란 영향을 주었으며, 반추위 내 pH의 저하는 혈 중 LPS의 농도가 증가로 이어짐을 확인할 수 있었다. 따라서 혈 중 LPS 농도는 급성 또는 만성형을 불문하고 과산증을 확인하는 지표로 사용 가능할 것으로 판단된다. 또한 착유우용 사료첨가물로 많이 이용되는 소디움벤토나이트(NaB)와 프로필렌글리콜(PG)의 급여는 반추위 내 pH의 저하를 완충시켜 궁극적으로 SARA의 강도를 완화시키고 혈 중 Lipopolysaccharides (LPS) 농도의 증가를 줄여 줌으로써 내독소에 의해 발생하기 쉬운 제엽염 등의 부작용을 완화시키는 데 도움을 줄 수 있을 것으로 사료된다.