• 동의신경정신과학회지
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• 제11권1호
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• pp.37-46
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• 2000

We investigated the effects of lemon pure essential oils on the heat shock-induced apoptosis in human astrocyte cell line CCF-STTG1. In previous studies, hear shock has been reported to induce the apoptosis or programmed cell death through the activation of caspase-3. Treatment of CCF-STTG1 cells with heat shock markedly induced apoptotic cell death as determined by flow cytometry. Interestingly, pretreatment of CCF-STTG1 cells with lemon pure essential oils inhibited the heat shock-induced apoptosis. Lemon also inhibited the heat shock-induced apoptosis in primary cultured rat astrocytes. To determine whether lemon inhibits the heat shock-induced activation of these apoptotic proteases, activation of CPP32 was assessed by Western blotting. Consistent with flow cytometry, DNA fragmentation and giemsa staining, heat shock-induced activation of CPP32 was blocked by lemon pure essential oil. PARP, cysteine protease substrates were fragmented as a consequence of apoptosis by heat shock. Lemon oil inhibited the PARP fragmentation. This essential oil also inhibited the heat shock-induced activation of caspase-3. These results suggest that lemon pure essential oils may modulate the apoptosis through the activation of the ICE-like caspases.

• Toxicological Research
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• 제21권3호
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• pp.249-253
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• 2005

The present study was carried out to investigate the skin irritation potential of lemon grass essential oil in rabbits. A volume of 0.5 ml of test article was applied to intact and abraded skins, respectively, for 24 h in 6 healthy male New Zealand White rabbits. Parameters measured during 72 h observation period were mortality, clinical signs, body weight changes, and local irritation. All rabbits showed severe edema at both 24 h and 72 h after the application of test article. These animals also showed very slight to severe erythema and/or slight eschar formation at both 24 h and 72 h. The edema and erythema induced by lemon grass essential oil were recovered on day 7 of additional 11-day recovery period, but the eschar formation was not recovered at the end of recovery period. On the other hands, there were no treatment-related adverse effects on clinical sign, body weight and gross finding in rabbits. Based on these results, it was concluded that a single dermal application of the undiluted lemon grass essential oil caused very slight to severe edema, erythema and eschar formation in rabbits and showed a primary irritation index score of 5.63 indicating severe irritation. The results of this study strongly suggest that lemon grass essential oil should be used with care and in highly diluted forms especially when directly applied to the skin.

• 한국응용과학기술학회지
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• 제24권2호
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• pp.99-108
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• 2007

The cosmetic and toiletries are necessary health care & household for common life. However we need antiseptic which is effecting harmlessly to the human body. There are propolis, Lavender, Lemon, essential oil in the natural antiseptic materials. This work proceeded design Natural-antiseptic system with three materials as above-mentioned. Natural-antiseptic system was accomplished with propolis (2%), Lavender essential oil (0.3%), Lemon essential oil (0.3%) safety out of Polysorbate 20 (0.5%), Polysorbate 80 (0.5%), PEG (60) hydrogenated castor oil (0.45%), ethanol (5%). The antimicrobial test was experimented on E. coli and Bacillus stearothermophilus. In this antimicrobial test, we found that the effect of antisepsis against E. coli and Bacillus stearothermophilus with propolis 0.3%, Lavender essential oil 0.045% and Lemon essential oil 0.045% was improved. Therefore could expect Natural-antiseptic system product for moisturizing skin toner for face, nourishing essence and wet tissue for clean other things.

• 한국식품저장유통학회지
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• 제30권4호
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• pp.549-561
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• 2023

Recently, consumers have gained an interest in natural and minimally processed foods, inciting the food industry to consider using of natural products as preservatives. Strawberries are a widely consumed fruit but are also highly perishable. Therefore, in this study, the physicochemical properties of strawberries (Fragaria×ananassa) were evaluated after a 12-h treatment with lemon essential oil (Citrus×limon) or cinnamon essential oil (Cinnamomum cassia) vapor during storage at 22℃ for 4 days in an accelerated shelf-life study and 4℃ for 18 days in a validation study. Weight loss was blunted in fruit treated with oil vapor during the first days of storage (p<0.05). Lemon essential oil delayed fruit darkening (p<0.05) but reduced the firmness of strawberries (p<0.05). Strawberries treated with cinnamon essential oil had a higher concentration of reducing sugars (p<0.05), and a decrease of 16.7% visible decay, although the difference was insignificant. Oil vapor treatment did not alter the pH, organic acid content, or soluble solid content during storage compared to the control. Since lemon and cinnamon essential oils have well-documented antimicrobial properties, they may be suitable for the natural preservation of fruit. This study provides new information on using essential oil vapor treatment to preserve fruits, and potentially decrease fruit loss and waste.

• 생물환경조절학회지
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• 제9권2호
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• pp.79-84
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• 2000

Thymus속의 common thyme과 thyme의 담액수경 재배시 배양액의 이온농도를 달리하여 두 종의 생육과 품질을 비교하였다. 배양액의 이온농도는 1.2, 2.4, 4.8 그리고 $7.2mS.cm^{-1}$로 하였다. 두 종 모두 $0.5(EC=1.2\;mS.cm^{-1})$배와 1배의 낮은 이온농도 처리에서 생육이 좋았다. Common thyme과 lemon thyme의 생육은 배양액내 이온농도가 높아질수록 건물율은 증가했으나 초장, 근장과 생체중은 감소하였다. 엽록소 함량은 lemon thyme에서 더 높았으나 비타민 C의 함량과 정유함량은 common thyme에서 더 높았다. 두 종간의 주요 정유 성분 차이를 보면, common thyme의 주성분은 thymo과 carvacrol이고, lemon thyme은 geranio과 ${\alpha}-citral$로 각각 정유 성분의 50~70%를 차지하고 있다. 이들의 각 주성분의 함량은 생육이 좋았던 0.5배에서 가장 많았다. 결과적으로 수경재배시 common thyme과 lemon thyme 모두 허브 배양액 0.5배액으로 재배시 우수한 품질의 작물을 생산할 수 있었다.

• 대한화장품학회지
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• 제37권4호
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• pp.303-308
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• 2011

레몬 오일과 유칼립투스 오일은 노화방지, 살균작용과 같은 다양한 효능을 가진 것으로 알려져 있다. 그러나 특정 피부 상재균에 대한 항균 효과와 항산화 효과는 아직 연구가 미비한 상황이다. 따라서 본 연구에서는 DPPH 라디칼 소거능 실험과 paper disc 방법을 이용하여 단일 오일들과 혼합오일의 항산화와 항균 활성을 검증하고자 하였다. 레몬 오일은 높은 DPPH 소거능을 보였으나 유칼립투스 오일은 아주 약한 소거능을 보였다. 두 오일을 혼합한 경우에는 효과가 증대되지 않았다. Paper disc 기법을 이용한 항균 효과 시험에서는 두 오일 모두 Candida albicans (C. albicans) 와 Propionibacterium acnes (P. acne)에서 항균력이 우수하며 농도의존적인 효능이 있음을 알 수 있었다. 또한, 두 오일 을 혼합 하였을 때 P. acne에 대하여 항균 활성이 상승됨을 알 수 있었다. 본 연구의 결과를 종합적으로 볼 때, 레몬 오일과 유칼립투스 오일의 혼합 오일은 천연성분으로서 균으로 야기된 문제성 피부나 여드름 피부를 개선시키는데 효과적일 것으로 사료된다.

• 농약과학회지
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• 제14권4호
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• pp.339-346
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• 2010

본 연구는 생리활성이 높은 식물오일의(라벤더, 레몬유칼립투스 및 계피 오일) 친환경 살충소재의 활용가능성을 탐색하고자 급성독성(급성경구독성, 급성경피독성, 피부자극성 및 안점막자극성)시험을 수행하였다. 랫드를 이용한 급성경구독성시험결과 라벤더, 레몬 유칼립투스 및 계피 오일의 $LD_{50}$은 2,000 mg/kg bw 이상이었고, 급성경피독성시험결과 모든 시험물질의 $LD_{50}$이 4,000 mg/kg bw으로 나타났다. 피부자극성시험결과 라벤더와 레몬 유칼립투스 오일은 자극성이 없었고, 계피 오일은 중도의 자극성을 나타냈다. 안점막자극성시험결과 라벤더, 레몬 유칼립투스 오일은 자극성이 없었고, 계피오일은 중도의 자극성을 나타냈으나 세척시험 결과 자극성은 없었다. 따라서, 라벤더 및 레몬 유칼립투스 오일은 독성이 낮았으나, 계피 오일은 중도의 피부자극성 및 안점막자극성을 갖는 것으로 평가되었다.

• 대한화장품학회지
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• 제31권3호
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• pp.265-271
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• 2005

Citrus essential oil (bergamot, grapefruit, lemon, mandarin, petigrain)이 B16 melanoma 세포에서 melanin 생성에 미치는 영향과 RBL 2H3 비만세포에서 ROS 생성에 미치는 영향을 관찰하였다 5 종류의 citrus essential oil은 DPPH 라디칼 소거와 세포 증식 및 독성에서 대조군에 비해 유의한 변화를 일으키질 않았다. 정제한 tyrosinase 활성에 있어서 mandarin과 petigrain essential oil은 유의하게 tyrosinase 활성을 억제하였으나 bergamot은 전혀 억제하지 않았다. 이러한 결과는 mandarin과 petigrain essential oil이 tyrosinase 효소 억제 기전은 설명하지 못하지만 tyrosinase 효소에 직접적으로 작용하여 억제하는 것으로 보인다. B16 melanoma 세포에서 MSH에 의한 melanin 생성이 5 종류의 citrus essential oil에 의해 모두 농도 의존적으로 억제되었다. Bergamot essential oil은 tyrosinase 효소 활성을 직접적으로 억제하지는 못했지만 melanoma 세포에서 MSH에 의한 melanin 생성은 억제하는 것으로 나타났다. 다른 4종류의 citrus essential oil도 모두 tyrosinase 효소 활성을 억제하였으며, MSH에 의한 melanin 생성도 억제하였다. 한편 DCF-DA를 이용한 ROS 생성에 있어서는 madarin essential oil은 ROS 생성에 이렇다 할 영향을 주지 않았지만 다른 4개의 essential oil (Bergamot, Grapefruit, Lemon, Petigrain)은 농도 의존적으로 ROS 생성을 증가시켰다. 이상의 결과를 종합하여 볼 때 citrus essential oil은 MSH에 의한 melanin 생성을 억제하는 것으로 보아 미백제로서의 개발 가능성이 있는 것으로 사료된다.

• 대한약침학회지
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• 제13권1호
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• pp.53-61
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• 2010

Purpose : To investigate the effects of the lavender, lemon and eucalyptus oil mixture on the atopy dermatitis skin lesions induced on NC/Nga Mice by dinitrochlorobenzene (DNCB). Material and Method : For this purpose, we fabricated the oil mixture blending three essential oils (lavender, lemon, eucalyptus : ELL) with one carrier oil (jojoba) and apply it on the atopic dermatitis skin lesions of NC/Nga Mice. Atopic dermatitis in NC/Nga Mice was induced by DNCB treatment on the dorsal skin of mice for 8 weeks. The mixture of ratio of each essential oil drop was 1 (eucalyptus) : 2 (lemon) : 2 (lavender) and this mixture was blended with jojoba oil 50ml (0.025%). The ELL-ointment was supplied for 8 weeks. We evaluated the effects of ELL on cell viability of mouse lung fibroblast, clinical skin features and severity, the level of serum Immunoglobulin (Ig) E & Ig G1, Interleukin (IL)-4, IL-13 and Interferon (IFN)-$\gamma$. Results : ELL showed safety on the cell viability of mouse lung fibroblast compared with control group. The cell viability was measured by SRB method. The effects of ELL on clinical skin features and severity in DNCB-induced dermatitis model of NC/Nga mice was significant compared with control group. EEL also showed significant effects on clinical symptom score compared with control group. Serum IgE & IgG1 level and development of atopy dermatitis skin lesions were evaluated. Serum IgE & IgG1 production was significantly down-regulated in EEL group compared with control group. ELL also down-regulated the levels of IL-4 and IL-13, and up-regulated the level of IFN-$\gamma$ compared with control group significantly. Conclusion : ELL was effective on atopy dermatitis by modulating Th2 related factors.

• 한국연초학회지
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• 제25권1호
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• pp.7-11
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• 2003

Repellencies of herb plants and essential oils against alate Myzus persicae were investigated in a insect cage, a screen house, and a tobacco field. Numbers of nymphs deposited by alate or apterous Myzur persicae were lower on rosemary(Rosmarinus officinalis), silver thyme (Thymus vulgaris), and lemon balm(Melissa of fcianazis). Rosemary and silver thyme show little repellency against alate Myzus persicae on a nearby tobacco plant Rosemary oil, peppermint (Mentha piperita) oil, and ginger oil showed repellency against alate Myzus persicae, but spearmint (Mentha spicata) oil and lavender oil showed little repellency in a screen house. Rosemary oil, ginger oil, peppermint oil, lavender oil, and spearmint oil showed repellency against alate Myzus persicae in a tobacco field, but there was no significant difference among oils.