• Title/Summary/Keyword: limonene

Search Result 336, Processing Time 0.02 seconds

Studies on Efficient Extraction of Limonene from Citron and Immune-modulation Activity for Development of Environmentally Friendly Material (친환경 소재 개발을 위한 유자에서의 효율적 Limonene 추출 및 면역기능 조절활성에 관한 연구)

  • Ahn, Jong-Ho;Lim, Hyun-Hee;Hwang, Seong-Gu;Nam, In-Sik
    • Korean Journal of Organic Agriculture
    • /
    • v.28 no.4
    • /
    • pp.591-604
    • /
    • 2020
  • The objectives of this study were to find out the best condition of extracting methods of limonene from citron and to determine effects of limonene on immune modulation activity by measuring cytokine secretion using RAW 264.7 mouse macrophage cells. When distilled water was used as a solvent instead of organic solvents to extract limonene from citron, addition of refluxing process to simultaneous steam distillation extraction method was found to be much effective in extracting limonene. However, it required longer extraction time than using other organic solvents. Limonene extracts showed increased IL-β and IL-6 but decreased the TNF-α gene expression in limonene concentration dependant manner. However oral administration of limonene extracts to mice did not influence significantly compared to control in in vivo experiment. It might be due to that the mice were kept in well controlled and complete environment. Limonene, a natural material from citron has been approved to have a immune-modulation activity in the present study and have a potential as a feed additive that is environmentally friendly and no harmful. Further study with protected limonene, for example, for the protection of limonene from oxidation or bypass the ruminal degradation in order consequently to increase immune-modulation activity might be useful as a further research.

Effect of N-3, N-6 Fatty Acid and d-Limonene Treatment on Membrane Lipid Composition and Protein Kinase C Activity in Experimental Rat Hepatocarcinogenesis (쥐의 간 발암과정에서 N-3, N-6 지방산 섭취 및 d-Limonene 투여가 생체막 지질조성 및 Protein Kinase C 활성도에 미치는 영향)

  • 김미정;김정희
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.32 no.8
    • /
    • pp.1328-1336
    • /
    • 2003
  • This study was done to investigate the effects of n-3, n-6 fatty acid and d-limonene on the hepatic membrane lipid composition, protein kinase C (PKC) and glutathione S-transferase (GST) activities in experimental rat hepatocarcinogenesis. Sprague-Dawley female rats were fed with two different types of dietary oil for 20 weeks. Corn oil (CO) and sardine oil (SO) were used at 15% by weight as a source of n-6 and n-3 fatty acid, respectively. One week after feeding, rats were intraperitoneally injected twice with a dose of diethylnitrosamine (DEN, 50 mg/kg body weight) and after 1 week 0.05% phenobarbital (PB) was provided with drinking water. Membrane fractional lipid composition showed that the content of cholesterol was higher in 50 group than CO group and also significantly decreased by d-limonene. The content of phospholipid was increased by carcinogen treatment but not affected by dietary oils or d-limonene. Membrane C/PL molar ratio was significantly decreased by d-limonene or carcinogen treatment in 50 groups but not in CO groups. Fatty acid composition was changed by dietary oils but not by carcinogen treatment or d-limonene. Cytosolic PKC activity was not significantly different by dietary oils, d-limonene or carcinogen treatment. However, membrane PKC activity was significantly increased by carcinogen treatment and decreased by d-limonene. Cytosolic GST activity was affected by d-limonene or carcinogen treatment in all dietary groups. These data indicate that dietary oils, d-limonene and carcinogen treatment can not change much membrane phospholipid composition. But membrane C/PL molar ratio was changed by carcinogen treatment and d -limonene although the effect was different between dietary oils. Therefore, it is suggested that different dietary oils and d-limonene can somewhat modulate the changes of membrane fluidity and activities of membrane bound enzymes like membrane associated PKC during carcinogenesis.

Inhibition of Benzo(a)pyrene-Induced Mutagenicity and Lipid Peroxidation by d-Limonene and Cineole (Benzo(a)pyrene에 의한 돌연변미월성과 지질과산화에 미치는 d-Limonene과 Cineole의 엑제 효과에 대한 비교연구)

  • 강은미;박성배;김상건;정기화
    • Biomolecules & Therapeutics
    • /
    • v.1 no.1
    • /
    • pp.71-76
    • /
    • 1993
  • It has been reported that d-limonene inhibits chemical-induced rat mammary cancer by the mechanism of increases in detoxification enzymes such as glutathione S-transferases and that cineole fails to exhibit significant suppressive effect on chemical-induced carcinogenesis. The present study was designed to compare the effects of d-limonene and cineole on the benzo(a)pyrene (BP)-induced mutagenicity, BP metabolism and lipid peroxidation. Modified Ames assay was employed to evaluate the inhibitory effect of d-limonene and cineole on the BP-induced mutagenicity. The number of revertant-bearing wells was decreased by 44~77% in the presence of both BP and d-limonene compared with that of BP alone whereas cineole decreased the number of revertant-bearing wells by 28~45% at the concentrations between $2{\mu}m$m.TEX> and 2 mM. d-Limonene suppressed BP metabolism by 16, 54 and 67% at 1, 10 and 100 mM, respectively while cineole inhibited the metabolism by 16, 26 and 55% at the same concentrations. The $EC_{50}$ values for d-limonene and cineole in inhibiting lipid peroxidation were 2.0 mM and 16 mM respectively, as assayed by thiobarbituric acid method. The present study showed that d-limonene and cineole have common antimutagenic effects although d-limonone appeared to be more effective than cineole in suppressing mutation and lipid peroxidation. The results suggest that the antimutagenic effects of d-limonene and cineole may be associated with alternation in enzyme activities and with inhibition of lipid peroxidation.

  • PDF

Effects of Different Dietary Oil and d-Limonene on Histopathological and Biochemical Changes in Experimental Hepatocarcinogenesis (식이지방의 종류 및 d-Limonene 투여가 간 발암과정에 미치는 영향)

  • 이미숙;김정희
    • Journal of Nutrition and Health
    • /
    • v.33 no.1
    • /
    • pp.23-32
    • /
    • 2000
  • The purpose of this study was to investigate the effcts of n-3, n-6 fatty arid and d-limonene on histopathological and biochemical changes in experimental rat hepatocarcinogenesis. To attain the above objectives, weanling Sprague-Dawley female rats were intraperitoneally injected twice with a dose of diethylnitrosamine(DEN, 50mg/kg body weight) and after 1 week 0.05% phenobarbital was provided with water. Sardine oil rich in n-3 fatty acids and corn oil rich in n-6 fatty acids were fed at 15% by weight and 5% d-limonene was added to the diet in each group. Ten weeks or 20 weeks after DEN treatment, rats were sacrifirced. The formation of glutathione S-transferase placental form positive(GST-P$\^$+/) foci was significantly decreased by the treatment of either sardine oil or d-limonene HMG-CoA reductase activity was not affected by dietary oils and d-limonene. Protein kinase C (PKC) activity was decreased by either sardine oil or d-limonene. Particularly d-limonene decreased the membrane PKC activity. Membrane Cholesterol/Phospholipid(Chol/PL) ratio was significantly decreased by d-limonene in sardine oil group. The data showed that GST-P$\^$+/ foci number was positively correlated with membrane PKC activity and serum cholesterol and negatively correlated with liver cholesterol level. These results suggest informations about the correlation between histopathological and biochemical changes such as cholesterol metabolism and PKC activity in experimental hepatocarcinogenesis and thereby can elucidate the possible mechanism related to the cancer inhibition.(Korean J Nutrition 33(1) : 23-32, 2000)

  • PDF

Microbial Conversion of (+)-Limonene by an Enterobacter agglomerans Isolate

  • Park, Yeon-Jin;Kim, In-Cheol;Chang, Hae-Choon
    • Journal of Microbiology and Biotechnology
    • /
    • v.13 no.4
    • /
    • pp.636-639
    • /
    • 2003
  • Entercbacter agglomerans 6L was isolated from citron (Citrus junos) peel by using an enrichment culture containing (+)-limonene. It was able to metabolize limonene and grew well ($A_{600}$:4.5) on limonene as a sole carbon source. E. agglomernas 6L was highly resistant to limonene toxicity, and grew to 1.0 optical density ($A_{600}$) even at 5% (v/v) of limonene in Luria-Bertani media. ${\gamma}-Valerolactone$ and cryptone were detected as the major metabolic products of limonene by E. agglomerans 6L.

Limonene Inhibits Methamphetamine-Induced Sensitizations via the Regulation of Dopamine Receptor Supersensitivity

  • Gu, Sun Mi;Kim, Sung Yeon;Lamichhane, Santosh;Hong, Jin Tae;Yun, Jaesuk
    • Biomolecules & Therapeutics
    • /
    • v.27 no.4
    • /
    • pp.357-362
    • /
    • 2019
  • Limonene is a cyclic terpene found in citrus essential oils and inhibits methamphetamine- induced locomotor activity. Drug dependence is a severe neuropsychiatric condition that depends in part on changes in neurotransmission and neuroadaptation, induced by exposure to recreational drugs such as morphine and methamphetamine. In this study, we investigated the effects of limonene on the psychological dependence induced by drug abuse. The development of sensitization, dopamine receptor supersensitivity, and conditioned place preferences in rats was measured following administration of limonene (10 or 20 mg/kg) and methamphetamine (1 mg/kg) for 4 days. Limonene inhibits methamphetamine- induced sensitization to locomotor activity. Expression of dopamine receptor supersensitivity induced by apomorphine, a dopamine receptor agonist, was significantly reduced in limonenepretreated rats. However, there was no significant difference in methamphetamine-induced conditioned place preferences between the limonene and control groups. These results suggest that limonene may ameliorate drug addiction-related behaviors by regulating postsynaptic dopamine receptor supersensitivity.

Herbicidal Activity of Naturally Developed d-Limonene against Sicyos angulatus L. under the Greenhouse and Open Field Condition (온실 및 노지조건에서 천연물질 d-Limonene의 가시박에 대한 살초활성)

  • Kang, Chung-Kil;Oh, Young-Joo;Lee, Sang-Beom;Lee, Byung-Mo;Nam, Hong-Shik;Lee, Yong-Ki;Jee, Hyeong-Jin;Hong, Moo-Ki;Koo, Suk-Jin
    • Korean Journal of Weed Science
    • /
    • v.31 no.4
    • /
    • pp.368-374
    • /
    • 2011
  • This experiment was conducted to Investigate the herbicidal activity of naturally developed d-Limonene on the control of Sicyos angulatus L. which is destructive to biodiversity and ecosystem under greenhouse and open field condition. Among the tested concentrations, most efficacy of early foliar application(leaf stage with 1.5 and 3) of d-Limonene was found in $70mg\;mL^{-1}$, followed by $35mg\;mL^{-1}$, $17.5mg\;mL^{-1}$ in that order under greenhouse condition. The higher the d-Limonene concentration, the better the effect in greenhouse condition. In leaf stage with 2, 3 and 5 under open field condition, the higher the d-Limonene concentrations, the higher the weeding effect. The remarkable effect was, particularly, found in d-Limonene with $70mg\;mL^{-1}$ while little was found in the plants with d-Limonene with $35mg\;mL^{-1}$. In the case of 15 leaf stage of S. angulatus L. treated with d-Limonene under outside treatment effect was low. The treatment effect of d-Limonene under open field compared with greenhouse condition was more significant. It can be recommended that d-Limonene with $70mg\;mL^{-1}$ could control S. angulatus L. by 5 leaf stage.

Cloning of Four Genes Involved in Limonene Hydroxylation from Enterobacter cowanii 6L

  • Yang, Eun-Ju;Park, Yeon-Jin;Chang, Hae-Choon
    • Journal of Microbiology and Biotechnology
    • /
    • v.17 no.7
    • /
    • pp.1169-1176
    • /
    • 2007
  • Genes encoding proteins responsible for limonene catabolism were cloned from a limonene-degrading microorganism, Enterobacter cowanii 6L, which was isolated from citron (Citrus junos) peel. The 8.6, 4.7, and 7.7 kb fragments (CD3, CD4, and CD6) of E. cowanii 6L chromosomal DNA that confer to E. coli the ability to grow on limonene have been cloned and their corresponding DNA sequences were determined. Nine open reading frames (ORFs) were identified, and the four ORFs (921 bp of CD3-2; 1,515 bp of CD4-1; 1,776 bp of CD6-1; and 1,356 bp of CD6-2) that encode limonene hydroxylase were confirmed by independently expressing these genes in E. coli. FAD and NADH were found to stimulate the hydroxylation reaction if added to cell extracts from E. coli recombinants, and multiple compounds (linalool, dihydrolinalool, perillyl alcohol, (${\alpha}-terpineol$, and ${\gamma}-terpineol$) were the principal products observed. Our results suggest that the isolate E. cowanii 6L has a broad metabolic capability including utilization of limonene. This broad metabolic ability was confirmed by identifying four novel limonene hydroxylase functional ORFs in E. cowanii 6L.

Cytyotoxicity and Anti-Malassezia Activity of Limonene (Limonene의 세포독성과 항Malassezia 활성)

  • Lee, Jeong-Hyun;Lee, Jae-Sug
    • Microbiology and Biotechnology Letters
    • /
    • v.39 no.4
    • /
    • pp.387-389
    • /
    • 2011
  • A previous study of ours indicated that Citrus auranifoli oil possesses antifungal activity against Malassezia furfur and Malassezia pachydermatis. In this study, we evaluated the anti-M. furfur and M. pachydermatis activities of limonene, which is a major component of C. aurantifolia oil, using the disk diffusion method. We also examined cytotoxicity against human normal epithelial (Beas-2B) cells using the cytopathic effect reduction (CPE) method. The results revealed that the minimum fungicidal concentration (MFC) value of limonene is lower than the value for itraconazole. The MFC value of limonene was seen to be 7.81 ${\mu}g$/mL against M. furfur and 3.90 ${\mu}g$/mL against M. pachydermatis. MFC values of itraconazole against M. furfur and M. pachydermatis were 62.50 ${\mu}g$/mL and 31.25 ${\mu}g$/mL, respectively. In addition, it was noted that limonene was not toxic to Beas-2B cells with normal morphology at a concentration of 100 ${\mu}g$/mL. However, itraconazole exhibited weak toxicity at the same concentration. Therefore, our results indicate that limonene could potentially be effective at controlling M. furfur and M. pachydermatis infections with no cytotoxicity.

D-Limonene mitigate myocardial injury in rats through MAPK/ERK/NF-κB pathway inhibition

  • Younis, Nancy Safwat
    • The Korean Journal of Physiology and Pharmacology
    • /
    • v.24 no.3
    • /
    • pp.259-266
    • /
    • 2020
  • Cardiovascular diseases are the primary reason of mortality, among which myocardial infarction (MI) is the most dominant and prevalent. This study was considered to examine D-Limonene protective action against isoproterenol (ISO) induced MI. Wister male rats were dispersed into four groups. Normal and D-Limonene control group in which rats administered saline or D-Limonene. ISO control animals were administered saline for 21 days then challenged with ISO (85 mg/kg, subcutaneously) on 20th and 21st day for MI induction. D-Limonene pretreated group in which animals were pretreated with D-Limonene 50 mg/kg orally for 21 days then administered ISO on 20th and 21st day. MI prompted variations were assessed by myocardial infarction area determination, blood pressure (BP) alterations, cardiac injury biomarkers and inflammatory mediators measurements. For more depth investigation, both the apoptotic status was evaluated via measuring mRNA expression of Bcl-2 and Bax as well as mitogen-activated protein kinase-extracellular signal-regulated kinase (MAPK-ERK) signal transduction were investigated via Western blotting. MI group revealed significant infarcted area, blood pressure alterations, myocardial injury enzymes intensification together with inflammatory cytokines amplification. MI was associated with activation of MAPK-ERK signal pathway and apoptotic status within the myocardium. On the other hand, pretreated with D-Limonene demonstrated deterred infracted area, reduced myocardial enzymes, improved BP indices, lessened inflammatory levels. Furthermore, D-Limonene pretreatment caused a decline in MAPK proteins pathway and Bax relative mRNA expression, while intensifying Bcl-2 mRNA expression promoting that D-Limonene may constrain MI induced myocardial apoptosis. D-Limonene mitigated MI injury through MAPK/NF-κB pathway inhibition and anti-apoptotic effect.