• Journal of Nutrition and Health
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• 제21권3호
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• pp.154-163
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• 1988

Effects of alchohol and fat content in a balanced diet on chemical composition and morphology of liver were investigated in growing rats. Fourth eight male rats of Sprague-Dawley strain weighing about 160g were divided into 4 groups ; high fat diet group, alcohol-administered high fat diet group, low fat diet group and alcohol-administered high fat diet group, low fat diet group and alcohol-administered low fat diet group. High and low fat diets supplied 30% and 12%, respectively, of total calorie intake from fat, and alcohol was given by adding ethanol in drinking waster at 10%. Diets contained adequate amounts of all nutrients required for rats, including lipotrpoic agents(choline and methionine) to minimize effects of factors other than alcohol on liver damage. Ratios of liver weight to body weight were statistically different among groups. Liver/dody weight ratios alcohol-administered rats were significantly higher than those of non-alcohol groups after 6 weeks treatment. Although total lipid and triglyceride per gram liver were increased in alcohol-administered rats, especially low fat diet fed rats, the values were not significantly different. Opticmicroscopical observation revealed increase in cell size and no change in morphology of liver. Examination of hepatocytes by electron microscopy showed that fat droplets were observed in all groups but enlarged in the alcohol-administered low fat diet fed rat. Contents of protein, cholesterol and phospholipid were not affected by alcohol consumption. The level of lipid peroxide was significantly lower in the livers of alcohol-administered rats than in the livers of non-alcohol groups. The results of this study indicate that even moderate alcohol drinking and dietary fat content did not affect any significant change in composition and morphology of liver until 6 week treatment but that even moderate alcohol drinking caused some signs of steatosis of liver.

• 한국동물위생학회지
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• 제22권2호
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• pp.135-149
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• 1999

This study was concerned with assessment of dietynitrosamine (DEN) induced liver cell carcinogenesis by measurement of changes preceding the development of neoplasms. The changes of hepatic morphology in rats(Sprague-Dawley) were detected by hematoxylin-eosin stain and immunohistochemistry(PCNA). The results were as follows ; 1. Minor behavioral change, brittleness of hair and decreased amount of water and diet intake. were observed in rats 7 weeks after DEN administration. 2. Variable size of liver tumor and hepatomegaly were observed in rats treated with DEN after 10 weeks. 3. Numerous vacuoles were showed on the midzonal and or peripheral areas of hepatic lobules. The large and polymorphological hepatocytes with eosinophilic cytoplasm or densely basophilic mitotic nucleoli were showed. 4. Several proliferative small round cells were shown on vacuolated and necrotic areas in peripheral hepatic lobules or portal areas. 5. PCNA-positive cells were showed on the vacuolated portal areas and peripheral areas of hepatic lobules. Maximal positivity was 23.6% in the areas of small round cells. In conclusion, this result confirmed that the DEN was one of the potent hepatocarcinogens. In histopathological analysis, the altered foci, hyperplastic nodules, neoplastic nodules, adenomas and carcinomas were observed in liver tumors induced by administration of DEN in rats.

• 한국의학물리학회지:의학물리
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• 제15권2호
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• pp.70-76
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• 2004

간 영역을 다른 복부 장기들로부터 정확히 분할한 후 간 내부의 종양을 감별 분할하므로써 간암을 조기 발견하는 데 도움을 준다. 본 논문은 복부의 모포러지컬 특성을 이용하여 효과적인 완전 자동 간 분할을 수행할 수 있는 알고리즘을 제안한다. 전처리 단계로서 다봉성 히스토그램 분할을 수행하고 복부의 모폴러지 좌표를 찾기 위해 척추를 분할한다. 다음으로 간 영역을 C-class maximum a posteriori (MAP) decision과 이진 모폴러지 필터링에 의해 추출한다. 자동으로 분할된 간 영역을 평가하기 위해 영역 에러율(Average Error Rate)과 회전 이진 영역 투영 매칭법(Rotational Binary Region Projection Matching; RBRPM)에 의한 상관 계수를 사용한다. 실험 결과는 제안한 알고리즘에 의해 획득한 완전 자동 간 분할과 수동 간 분할사이에 매우 유사한 결과를 보였다.

• 한국식품영양과학회지
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• 제25권3호
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• pp.374-378
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• 1996

The effects of pine needle extracts on lipid contents and antioxidative enzyme activities in liver of rat were evaluated. Thirty male Sprague-Dawley rats were divided into five groups and fed high fat diets for four weeks. Each group was administered with pine needle extract in the following doses: control, water ; WE-3, hot water extract(3% PN) ; WE-6, hot water extract(6% PN) ; AE-3, acetone extract(3% PN) ; AE-6, acetone extract(6% PN). The results obtained from the experiment were as follows: GOT activities were not significantly different among experimental groups but GPT activities were significantly low in the experimental groups compared to the control group. Liver superoxide dismutase(SOD) activity of pine needle extract administered groups was higher than that of control group. Catalase activities of liver had a similar tendency to SOD activities, but were not significantly different among the groups. Liver TBARS of WE-3 WE and AE-6 groups were slightly lower than those of other groups. Microscopic observation of liver tissue revealed that pine needle extracts increased cellular swelling, which was markedly increased in WE-6 group compared with control group.

• Animal cells and systems
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• 제15권1호
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• pp.19-27
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• 2011

Hepatocytes, the major epithelial cells of the liver, maintain their morphology in culture dishes coated with extracellular matrix (ECM) components such as collagen and fibronectin or biodegradable polymers (e.g. chitosan, gelatin). In these coated dishes, survival of cells and maintaining of liver-specific functions may increase. The aim of this study was to determine a suitable, cost-effective and simple system for hepatocyte isolation and culture which may be useful for various applications such as in vitro toxicology studies, hepatocyte transplantation and bioartificial liver (BAL) systems. In order to obtain primary cultures, hepatocytes were isolated from liver by an enzymatic method and cultured on plates coated with collagen, chitosan or gelatin. Collagen, gelatin-sandwich and gelatin-cell mixture methods were also evaluated. Morphology and attachment of the cells were observed by inverted microscope and scanning electron microscope (SEM). An MTT assay was used to determine cell viability and mitochondrial activity.

• 대한핵의학회:학술대회논문집
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• 대한핵의학회 1971년도 제10차 학술대회
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• pp.81.1-81.1
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• 1971

• 대한의생명과학회지
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• 제11권3호
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• pp.333-336
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• 2005

Environmental endocrine disruptors are very toxic to the animals including humans. They are hormone-like acting chemicals which can be found in our normal daily life. We have examined the toxic effect of bisphenol A and if lactic acid bacteria could suppress this toxic effect. Thirty rats were divided into three groups (control, bisphenol A treated, bisphenol A and lactic acid bacteria treated). Treatments were carried out at an interval of 12 hours for each group. Control group showed normal and clear morphology of tissues. Cells were fine in their shape and color, and density was high enough for the normal function. However, bisphenol A treated group was abnormally destructed in cell morphology. In the testis, sperms were totally destructed. When treated with lactic acid bacteria together, the toxic effect of bisphenol A was clearly decreased. This study indicated that bisphenol A was toxic in any concentration especially for the liver and testis, however, lactic acid bacteria could suppress the toxic effects of bisphenol A.

• 한국식품영양과학회지
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• 제33권5호
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• pp.815-820
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• 2004

Orotic acid(OA)-유발 지방간 흰쥐의 혈청 및 간장의 지질농도, 임상생화학적 및 조직형태학적 변화에 미치는 cap-saicin(CS)성분의 영향을 검토하였다. 기본식이에 OA 1%, CS 0.02% 및 OA와 CS를 각각 1%와 0.02% 수준으로 첨가하여 4주간 섭취 시켰다. 간장 중성지방 농도는 대조군에 비해 약 4.7배 현저히 증가하여 지방간이 유발되었으나, OA十CS 동시투여에 의해서는 간장 중성지방 농도가 OA군에 비해 38% 감소하여 지방간이 개선된 것으로 나타났다. OA 식이에 의해 간장 콜레스테롤 농도가 증가하였으나, capsaicin 동시투여에 의해 감소하였다. Capsaicin 단독 투여는 간장 중성지방 및 콜레스테롤 농도에 영향을 미치지 못하였다. Capsaicin 투여에 의한 혈청 중성지방 및 콜레스테롤 농도의 감소는 OA 첨가 또는 무첨가 식이군에서 모두 관찰되었다. 간기능 지표 효소인 혈청 GOT 및 GPT 활성은 각 실험군간에 차이가 나타나지 않았다. 간조직의 형태학적 관찰 결과, 대조군과 CS군은 정상적인 간소견을 보였으나 OA군의 간세포에는 소포성 지방 변성이 소엽 중심대로 주로 나타나 있고, 크고 많은 지방세포들이 균일하게 배열되어 있었다. 그러나 OA+CS군의 간세포들은 손상된 세포가 재생될 뿐 아니라 소포성 지방세포들의 크기와 수가 줄어들고 있음을 확인하였다. 이상의 실험 결과에서, capsaicin 0.02% 농도는 OA-유발 지방간을 개선시키는 효과가 있는 것으로 판단되어진다.

• 한국정보통신학회논문지
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• 제16권8호
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• pp.1765-1772
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• 2012

의료기기 및 진단 기술의 발달로 신체 장기의 이식에 대한 성공률이 향상되었으며 특히 간 기능 장애에 의한 간이식이 늘어나는 추세이다. 영상처리 및 분석의 발달로 간 이식을 위한 간의 체적을 구하는 방법들이 정확성과 효율성이 높아졌다. 본 논문은 각 알고리즘들의 신속한 비교 및 분석, 빠른 프로토타입 개발에 효과적인 MeVisLab을 사용하여 간 영역을 분할하고 재구성하였다. 원본 영상에 문턱치 값 적용과 영역 확장법을 적용하여 간 영역을 분할하고 Morphology와 구멍 채우기, 관심영역 설정으로 노이즈 및 불필요한 객체를 제거하여 간을 분할하였다. MeVisLab의 사용으로 높은 시간적 효율과 다양한 비교 및 분석 모듈 사용 방법을 제시하여 의료영상처리 연구의 저변 확대에 기여하리라 판단된다.

• Applied Microscopy
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• 제28권3호
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• pp.283-297
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• 1998

Hemopoiesis and morphogenesis of the human fetal liver through from 10 to 32 weeks of gestation were investigated by light and electron microscopy. The results obtained were as follows. Hemopoiesis of fetal liver tissue was found from 10 to 32 weeks of gestation, but the hemopoiesis was decreased at 32 weeks of gestation. At the 32 weeks of gestation, matured erythrocytes were observed in the sinusoid, and formation of liver cell cord and portal triad were established. Differentiation of hepatic cell was characterized by the increase of amount of cell organelles within cytoplasm, decrease of hemopoietic cell, morphological change of nuclear envelope from folding form to round form during the developmental period. These results suggest that human fetal liver plays a hematopoietic function until bone marrow and spleen play their function, but morphology of liver at 32 weeks of gestation was differed with structure observed in liver of adult.