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검색결과 15건 처리시간 0.021초

Establishment of Quantitative Analysis Method for Genetically Modified Maize Using a Reference Plasmid and Novel Primers

  • Moon, Gi-Seong;Shin, Weon-Sun
    • Preventive Nutrition and Food Science
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    • 제17권4호
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    • pp.274-279
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    • 2012
  • For the quantitative analysis of genetically modified (GM) maize in processed foods, primer sets and probes based on the 35S promoter (p35S), nopaline synthase terminator (tNOS), p35S-hsp70 intron, and zSSIIb gene encoding starch synthase II for intrinsic control were designed. Polymerase chain reaction (PCR) products (80~101 bp) were specifically amplified and the primer sets targeting the smaller regions (80 or 81 bp) were more sensitive than those targeting the larger regions (94 or 101 bp). Particularly, the primer set 35F1-R1 for p35S targeting 81 bp of sequence was even more sensitive than that targeting 101 bp of sequence by a 3-log scale. The target DNA fragments were also specifically amplified from all GM labeled food samples except for one item we tested when 35F1-R1 primer set was applied. A reference plasmid pGMmaize (3 kb) including the smaller PCR products for p35S, tNOS, p35S-hsp70 intron, and the zSSIIb gene was constructed for real-time PCR (RT-PCR). The linearity of standard curves was confirmed by using diluents ranging from $2{\times}10^1{\sim}10^5$ copies of pGMmaize and the $R^2$ values ranged from 0.999~1.000. In the RT-PCR, the detection limit using the novel primer/probe sets was 5 pg of genomic DNA from MON810 line indicating that the primer sets targeting the smaller regions (80 or 81 bp) could be used for highly sensitive detection of foreign DNA fragments from GM maize in processed foods.

A New Latent Class Model for Analysis of Purchasing and Browsing Histories on EC Sites

  • Goto, Masayuki;Mikawa, Kenta;Hirasawa, Shigeichi;Kobayashi, Manabu;Suko, Tota;Horii, Shunsuke
    • Industrial Engineering and Management Systems
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    • 제14권4호
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    • pp.335-346
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    • 2015
  • The electronic commerce site (EC site) has become an important marketing channel where consumers can purchase many kinds of products; their access logs, including purchase records and browsing histories, are saved in the EC sites' databases. These log data can be utilized for the purpose of web marketing. The customers who purchase many product items are good customers, whereas the other customers, who do not purchase many items, must not be good customers even if they browse many items. If the attributes of good customers and those of other customers are clarified, such information is valuable as input for making a new marketing strategy. Regarding the product items, the characteristics of good items that are bought by many users are valuable information. It is necessary to construct a method to efficiently analyze such characteristics. This paper proposes a new latent class model to analyze both purchasing and browsing histories to make latent item and user clusters. By applying the proposal, an example of data analysis on an EC site is demonstrated. Through the clusters obtained by the proposed latent class model and the classification rule by the decision tree model, new findings are extracted from the data of purchasing and browsing histories.

편의점에서 판매되는 김밥 및 샌드위치의 냉장조건에서의 유통기한 (Shelf-life of Prepacked Kimbab and Sandwiches Marketed in Convenience Stores at Refrigerated Condition)

  • 구민선;김윤숙;신동빈;오세욱;전향숙
    • 한국식품위생안전성학회지
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    • 제22권4호
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    • pp.323-331
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    • 2007
  • 편의점에서 판매하는 김밥류 6종, 샌드위치류 6종에 대해 $10^{\circ}C$ 냉장 조건하에서 72시간 저장하면서, 품질변화를 분석하여, 품질 지표를 도출하고, 유통기한을 예측하였다. 저장 기간 중 시료의 단백질의 부패도를 나타내는 휘발성 염기질소의 함량은 모든 시료에서 큰 변화를 보이지 않았다. 저장 중 김밥류의 휘발성염기질소는 저장 직전인 초기 $1.3{\sim}4.1mg%$에서 저장 72시간 후 $3.7{\sim}6.9mg%$로 약간 증가하였고, 샌드위치도 초기 $1.5{\sim}10.3mg%$에서 저장 72시간 후 $3.1{\sim}12.3mg%$로 증가하여 큰 변화를 보이지 않았다. 대장균, 황색포도상구균, 살모넬라균, 비브리오균은 저장기간 동안 모든 시료에서 검출되지 않았다. 일반세균수는 저장 초기 김밥류에서는 $2.9{\sim}4.43\;logCFU/g$, 샌드위치는 $2.34{\sim}2.92\;logCFU/g$였으나 저장 48시간 후에는 김밥류에서 $4.51{\sim}5.36\;logCFU/g$로, 샌드위치에서는 $4.66{\sim}5.02\;logCFU/g$로 증가하였고, 48시간 이후에는 대부분의 시료에서 급격히 증가하였다. 각 제품의 종합적 기호도를 검사한 결과 일반김밥 4종은 저장 40시간까지 5점 이상을 유지하였고, 삼각김밥은 48시간까지, 샌드위치는 저장 42시간까지 상품성을 가지는 5점을 유지하였다. 저장기간과 일반세균수 및 관능검사와의 상호관계를 회귀분석을 통하여 상관관계를 측정한 결과, 대부분이 0.9가 넘는 높은 상관관계를 보여주었다. 유통가능 기준인 관능 검사의 종합적 기호도 5점 이상, 일반세균수 $10^5CFU/g$ 이하의 조건을 만족하고 유통조건중의 안전성을 고려한 유통기한을 산출한 결과 $10^{\circ}C$ 냉장조건 하에서 일반 김밥은 $15{\sim}33$시간, 삼각김밥은 $32{\sim}33$시간, 샌드위치류는 $27{\sim}30$시간이었다.

BERT 기반 감성분석을 이용한 추천시스템 (Recommender system using BERT sentiment analysis)

  • 박호연;김경재
    • 지능정보연구
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    • 제27권2호
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    • pp.1-15
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    • 2021
  • 추천시스템은 사용자의 기호를 파악하여 물품 구매 결정을 도와주는 역할을 할 뿐만 아니라, 비즈니스 전략의 관점에서도 중요한 역할을 하기에 많은 기업과 기관에서 관심을 갖고 있다. 최근에는 다양한 추천시스템 연구 중에서도 NLP와 딥러닝 등을 결합한 하이브리드 추천시스템 연구가 증가하고 있다. NLP를 이용한 감성분석은 사용자 리뷰 데이터가 증가함에 따라 2000년대 중반부터 활용되기 시작하였지만, 기계학습 기반 텍스트 분류를 통해서는 텍스트의 특성을 완전히 고려하기 어렵기 때문에 리뷰의 정보를 식별하기 어려운 단점을 갖고 있다. 본 연구에서는 기계학습의 단점을 보완하기 위하여 BERT 기반 감성분석을 활용한 추천시스템을 제안하고자 한다. 비교 모형은 Naive-CF(collaborative filtering), SVD(singular value decomposition)-CF, MF(matrix factorization)-CF, BPR-MF(Bayesian personalized ranking matrix factorization)-CF, LSTM, CNN-LSTM, GRU(Gated Recurrent Units)를 기반으로 하는 추천 모형이며, 실제 데이터에 대한 분석 결과, BERT를 기반으로 하는 추천시스템의 성과가 가장 우수한 것으로 나타났다.

정신분열병 환자에서 신체미세기형에 관한 연구 (Minor Physical Anomalies in Patients with Schizophrenia)

  • 주은정;정성훈;맹소진;윤세창;김종훈;김철응;신영민;김용식
    • 생물정신의학
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    • 제9권2호
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    • pp.140-151
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    • 2002
  • Object and Method:Minor physical anomalies(MPAs) are frequently seen in patients with schizophrenia. MPAs are considered to arise from the anomalous development of ectoderm-originated tissues in the developing fetus. Since the central nervous system originates from ectoderm, MPAs can be regarded as externally observable and objective indicators of the aberrant development which might have taken place in the central nervous system. To investigate whether MPAs are more frequent in schizophrenic patients, the frequencies of MPAs were compared between schizophrenic patients and normal controls. Total 245 schizophrenic patients diagnosed with DSM-IV(male : 158, female : 87), and 418 normal control subjects(male : 216, female : 202) were included in this study. The MPAs were measured using the modified Waldrop scale with fifteen items in six bodily regions; head, eye, ear, mouth, hand, and foot. Result:The total scores of Waldrop scale were $4.40{\pm}1.93$($mean{\pm}standard$ deviation) in patients and $3.43{\pm}1.68$ in controls for females, and for males, $4.58{\pm}1.75$ in patients and $4.28{\pm}1.59$ in controls. For females, the excess of MPAs in schizophrenic patients was statistically significant(t-test : p<0.001). For males, schizophrenic patients also showed more MPAs than normal controls, but this tendency did not reach statistical significance (t-test : p=0.094). When the modified Waldrop total scores excluding head circumference were compared, the total scores in schizophrenic patients were significantly higher for both male and female subjects(t-test : male p<0.001, female p=0.001). The individual anomaly items included in Waldrop scale were also investigated. The items of epicanthus, hypertelorism, malformed ears, syndactylia were significantly more frequent in schizophrenic patients. In contrast, the items of adherent ear lobes, asymmetric ears, furrowed tongue, curved fifth finger, single palmar crease and big gap between toes did not show any differences in frequency between schizophrenic patients and normal controls. Since a lot of statistical analyses showed different results between male and female subjects, it seems to be necessary to consider gender as an important controlling variable for the analysis, however only the item of head circumference showed statistically significant gender-related difference according to log-linear analysis. Conclusion:With a relatively large sample size, the frequencies of MPAs enlisted in Waldrop scale were compared between schizophrenic patients and normal controls in this study. MPAs were more frequently seen in schizophrenic patients and, especially, several specific items in the Waldrop scale showed prominent excess in schizophrenic patients. Although definite conclusions cannot be drawn due to the inherent limitation of the study using Waldrop scale, these results seem to support the possibility that aberrant neurodevelopmental process might be involved in the pathogenesis of schizophrenia in some of the patients.

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