• Title/Summary/Keyword: lymphocyte proliferation

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The effect of Astragali Radix and Armeniacae Semen on the apoptosis of immunocytes and nitric oxide production from peritoneal macrophages (황기와 행인(杏仁)이 면역세포(免疫細胞)의 Apoptosis 및 Nitric Oxide에 미치는 효과(效果))

  • Jeong Hyun-Woo;Moon Han-Ju
    • Herbal Formula Science
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    • v.6 no.1
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    • pp.175-186
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    • 1998
  • The purpose of this research was to investigate effects of Astragali Radix(AR) and Armeniacae Semen(AS) on T-lymphocytes and peritoneal macrophages in mice. The proliferation of thymocytes and splenocytes were teated using macroplate-reader. The apoptosis and sub-population of T-lymphocytes were tested using a flow cytometer. Nitric oxide production was tested using a Griess reagents. The result were obtained as follow; 1. AR increased the proliferation of thymocytes and splenocytes. 2. AS decreased the proliferation of thymocytes and splenocytes. 3. AR and AS decreased No production fron peritoneal macrophages 4. AR and AS were accelerate T-lymphocytes apoptosis. 5. AR and AS increased $T_C$ cells population, but decreased $T_H$ cells population of T-lymphocyte.

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Immunomodulating Activities of Brazilin in vitro

  • Moon, Chang-Kiu;Mock, Myung-Soo;Yang, Kyung-Mee;Han, Hye-Seung;Won, Hyeon-Soon;Kim, Ji-Young;Chung, Jin-Ho;Moon, Chang-Hyun
    • Archives of Pharmacal Research
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    • v.15 no.4
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    • pp.283-288
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    • 1992
  • This work was performed to investigate the effects of brazilin in vitro on mitrogen-induced proliferation, ConA-induced TCGF release and responsiveness to recombinant-induced proliferation, ConA-induced TCGF release and responsiveness to recombinant IL-2 using splenocytes from C57BL/6 female mice. Brazilin (29-80 ng/ml) caused a noticeable increase in TCGF production of splenocytes, but did not affect responsivness to recombinant IL-2, the expression of ConA-induced high affinity IL-2 receptor and mitogen-induced proliferation of splenocytes.

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Effects of Subacute Oral Administration of Bisphenol A on the IgM-PFC and Proliferation of Splenocytes in Mice (마우스에서 Bisphenol A의 아급성노출이 IgM-PFC형성능과 비장세포 증식능에 미치는 영향)

  • 변정아;표명윤
    • Environmental Analysis Health and Toxicology
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    • v.18 no.3
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    • pp.231-235
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    • 2003
  • To determine whether or not bisphenol A affects the Immune system, female ICR mice were treated bisphenol A (BPA) orally at the doses of 100, 500 and 1,000 mg/kg for 30 consecutive days. Four days before enumerating Plaque -forming cells (PFCs) mice were immunized intraperitoneally with sheep red blood cells (SRBCs). The spleen cellularity and PFC/spleen were significantly reduced by 30-day exposure to BPA (1,000 mg/kg/day), but the PFC/10$\^$6/ spleen cells was slightly decreased.. When splenocytes isolated from the mice exposed to BPA for 30 days were cultured in the presence of LPS, Con A or PHA with IL-2, the lymphocyte proliferation ex vivo was not significantly suppressed by BPA. Our present results indicated that 30-day exposure of mice to BPA might have mild immunotoxic potential.

Immunomodulating Activity of a Polysaccharide Isolated from Mori Cortex Radicis

  • Kim, Hwan-Mook;Han, Sang-Bae;Lee, Ki-Hoon;Lee, Chang-Woo;Kim, Chul-Young;Lee, Eun-Ju;Huh, Hoon
    • Archives of Pharmacal Research
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    • v.23 no.3
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    • pp.240-242
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    • 2000
  • The immunomodulating activity of a polysaccharide isolated from Morus alba (PMA) root bark was examined in murine splenic lymphocytes. PMA enhanced proliferation of splenic lymphocytes in a synergistic manner in the presence of mitogens. However, PMA suppressed pri-may IgM antibody production from B cells, which was activated with lipopolysaccharide, a polyclonal activator, or immunized with a T-cell dependent antigen sheep red blood cells. Our observations showed that the immunomodulating activity of PMA increased lymphocyte proliferation and that PMA decreased antibody production from B cells, which was distinct from those of other plant-originated polysaccharides.

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Lipid A of Salmonella typhimurium Suppressed T-cell Mitogen-Induced Proliferation of Murine spleen Cells in the Presence of Macrophage (Salmonella typhimurium lipid A를 처리한 식세포 존재 조건에서 mitogen에 유도되는 이자 세포의 증식억제)

  • Kang, Gyong-Suk;Chung, Kyung-Tae
    • Journal of Life Science
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    • v.17 no.1 s.81
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    • pp.31-38
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    • 2007
  • Infection with virulent or attenuated Salmonella typhimuriumhas known to induce reduction in proliferative responses of spleen cells. We investigated a role of lipid A from S. typhimurium, a B cell mitogen, on proliferation of spleen cells by T cell mitogens such as concanavaline A and phytohemagglutinin under in vitro and ex vivo conditions. Lipid A alone induced proliferation of spleen cells in vitroin a dose-dependent manner. However, subsequent treatment of concanavaline A or phytohemagglutin in after lipid A treatment induced proliferation suppression of murine spleen cells in vitro and ex vivo. Removal of macrophages from spleen cells, which were obtained from a lipid A-injected mouse, restored proliferation by concanavaline A and phytohemagglutinin, indicating that macrophages appeared to play a role in lipid A-induced suppression. Secreted molecules from macrophages did not accounted for the suppression because suppressive effect was not achieved when the supernatant from macrophage-containing spleen cell culture was conditoned to macrophage-depleted spleen cell culture. Co-culture of spleen cells from lipid A-treated and - untreated mice showed proliferation suppression as increasing cell numbers of lipid A-treated mouse. These data suggested that the cell-to-cell contact of macrophage with splenic lymphocyte cells is responsible for immune responses against lipid A, which is applicable to the case of human S. typhi infection.

Effect of capsaicin on murine lymphocyte functions and lymphoid tissue morphology

  • Lee, June-Chul;Park, Yeong-Min
    • IMMUNE NETWORK
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    • v.1 no.3
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    • pp.203-212
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    • 2001
  • Background: Rapid advances in neuroendocrine immunology have established the concept of bidirectional communication between the immune and neuroendocrine systems. Capsaicin suppresses the immune function by destroying substance P acting as mediatior of neuroendocrine immune system. Methods and Results: In this study, effect of capsaicin on mature murine lymphocyte functions and lymphoid tissue morphology was examined. Formally, capsaicin showed the strong cytotoxic effect on splenocyte over $10{\mu}g/ml$ concentration in citro. And proliferation and Th1-cytokine expression of splenic cells in mice that received high dose of capsaicin ($100{\mu}g/mouse$) were significantly diminished. However, low dose of capsaicin treatment did not influence these responses in vivo($1{\mu}g/mouse$) and in vitro (under $5{\mu}g/ml$). And the morphology of spleen and lymph nodes after capsaicin treatment was observed. In the spleen of mice injected with high dose of capsaicin (100, $200{\mu}g/mouse$), the size of white pulp was significantly decreased and the length of red pulp was increased, Moreover, vascularity index was diminished in a dose dependent manner. Conclusion: These results implies that immunosuppressive effect of capsaicin is associated with cytotoxic activity on lymphocyte, Th1-cytokine down-regulation and lymphoid tissue abnormalization, and this report is expected to give a hand to the study for the mechanism of action of neurotoxin of the immune system.

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Immune activation and radioprotection by Echinacea purpurea (American herb)

  • Mishima, Satoshi;Gu, Yeun-Hwa;Saito, Kiyoto;Yamashita, Takenori;Maruyama, Hiroe;Inoue, Makoto;Ahn, Kyoo-Seok
    • Advances in Traditional Medicine
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    • v.4 no.3
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    • pp.163-170
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    • 2004
  • The effect of immune activation by Echinacea purpurea was investigated by measuring total immunoglobulin (Ig) G, IgM. and the radioprotective effect of immune activation by Echinacea purpurea was investigated by measuring T lymphocyte subsets in the peripheral blood of mice following whole body irradiation. Echinacea purpurea activated macrophages to stimulate $IFN-{\gamma}$ production in association with the secondary activation of T lymphocytes, resulting in a decrease in IgG and IgM production. Cytokines released from macrophages in mouse peripheral blood after Echinacea purpurea administration activated helper T cells to proliferate. In addition, activated macrophages in association with the secondary T lymphocyte activation increased $IFN-{\gamma}$ production and stimulated proliferation of cytotoxic T cells and suppressor T cells, indicating the activation of cell-mediated immune responses.

Extracts from Polypodium ferns upregulate the expression of CD95 in human peripheral blood lymphocytes

  • Lombardi, Valter R.M.;Etcheverria, I.;Fernandez-Novoa, L.;Blanco, A.;Diaz, J.;Cacabelos, R.
    • Advances in Traditional Medicine
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    • v.3 no.2
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    • pp.90-99
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    • 2003
  • There are several data in the literature indicating a great variety of pharmacological activities of Polypodium genus, which exhibit antiinflammatory and immunomodulatory activities. Since one of our main interests is to obtain natural immunoregulatory agents devoid of pharmacological adverse effects, we used flow cytometry analysis to highlight relative contributions of a water-soluble fraction of different concentrations of Polypodium rhizome extracts on lymphocyte subpopulations, NK and LAK activity. To measure their potential immunoregulatory activity a T cell proliferation assay in response to phytohemaglutinin (PHA) and mixed lymphocyte reactions were chosen. As a confirmatory bioassay we studied the effect of our extracts on CD45RO and CD95 antigen expressions. The results indicate that CD95 expression dramatically increases after peripheral blood lymphocyte activation and treatment with Polypodium leucotomus, cambricum and vulgare extracts, suggesting a powerful intrinsic pro-apoptotic effect.

Subpopulation of lymphocytes in Korean native cattle infected with enzootic bovine leukosis

  • Yoon, Soon-seek;Bae, You-chan;Jean, Young-hwa;Seo, Kook-hyun;Han, Hong-ryul
    • Proceedings of the Korean Society of Veterinary Pathology Conference
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    • 2003.10a
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    • pp.50-50
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    • 2003
  • Enzootic bovine leukosis(EBL) is chronic disease caused by bovine leukemia virus(BLV), retroviridae. The characteristic feature of this disease is proliferation of lymphocytes in circulating blood or lymphoid tissues. Because EBL concern lymphocytes, immunological disorder or alteration in the lymphocyte subpopulation is suggested. In this study, we investigated the changes of the lymphocyte subpopulation in the circulating blood of Korean native cattle infected with bovine leukemia virus. (omitted)

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Inhibitory effect of bisphenol A on the mixed lymphocyte reaction and TNF-$\alpha$ production of antigen presenting cells in mice

  • Hwang, Yoo-Kyung;Byun, Jung-A;Pyo, Myoung-Yun
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.166.1-166.1
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    • 2003
  • We investigated the effects of bisphenol A (BPA), endocrine disruptor, on the mixed lymphocyte reaction and TNF-$\alpha$ production of antigen presenting cells in mice. Cells from mouse (C57BL/6) bone marrow were cultured with GM-CSF for 8 days and mature dendritic cells (DCs) were prepared. These DCs proliferation in response to Balb/c splenocytes was measured at 72 h of culture with BPA by tritiated thymidine incorporation ([3H]TdR) and [3H]TdR incorporation was determined by scintilation counting. (omitted)

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