• 한국수산과학회지
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• 제20권2호
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• pp.136-145
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• 1987

말똥 성게를 인공 수정시킨 후 column chromatography법으로 DNA polymerase $\alpha$를 분리 정제하였다. Sephadex G-200과 SDS polyacryamise gel electophoresis에 의한 DNA Polymerase $\alpha$의 분자량은 약 $137,000\~138,000$이였다. 효소활성의 최적 pH는 7.4였고, 칼슘이온 20mM, 나트륨이온 25mM에서 활성이 높았고, 마그네슘 이온은 10 mM 일 때 활성이 높았다. DNA polymerase $\alpha$는 N-ethylmaleimide, aphidicolin, cytosin $\beta-D-arabinofuranoside$ 5'-triphosphate (ara CTP)와 phosphonoacetic acid체 의하여 활성이크게 저하되었다.

• 농약과학회지
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• 제4권2호
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• pp.72-75
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• 2000

제초제 후부물질로서 3,4-Dimethyl-N-[4-chloro-2-fluoro-5-{(pyrazolylmethyl)oxy}phenyl]maleimide 유도체들과 또는 3,4,5,6-tetrahydro-N-[4-chloro-2-fluoro-5-{(pylazolylmethyl)oxy}phenyl]phthalimide 유도체들을 합성하여 제초활성을 온실조건에서 평가하였다. 이들 중 N-methyl-5-pyrazolylm-ethyloxy를 갖는 화합물들이 처리량 60 g/ha이하에서 이앙벼에 안전하면서 논피에 강한 제초활성을 나타내었다.

• Macromolecular Research
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• 제17권12호
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• pp.1003-1009
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• 2009

Copolymers of N-vinylpyrrolidone (NVP) comonomer with styrene (St), hydroxypropyl methacrylate (HPMA) and carboxyphenyl maleimide (CPMI) were synthesized by free radical polymerization using 2,2'-azobisisobutyronitrile (AIBN) initiator in 1,4-dioxane solvent. The copolymers formed were characterized by FTIR, $^1H$ NMR and $^{13}C$ NMR techniques and their thermal properties were studied by DSC and TGA. Copolymer composition was determined by $^1H$ NMR and/or by elemental analysis and monomer reactivity ratios (MRR) were estimated by the linear methods of Kelen-Tudos (K-T) and extended Kelen-Tudos (EK-T) and the non-linear approach. Copolymers of St and HPMA with NVP formed blocks of one of the monomer units, whereas alternating copolymers were obtained in CPMI-NVP, depending upon the side chain substitution. The MRR values are discussed in terms of monomer structural properties such as electronegativity and electron delocalization. The sequence distribution of monomers in the copolymers was studied by statistical method based on the average reactivity ratios obtained by EK-T method.

• 생명과학회지
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• 제9권6호
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• pp.646-652
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• 1999

The effect of temperature on the structure change of the SH of myosin head have been investigated with improved resolution by x-ray diffraction using synchrotron radiation. The movement of myosin head and conformational change of contractile molecules were occurred in the muscle contraction. IASL (iodo acetamide) and MSL (maleimide) disordered the orderly helix arrangement of myosin in the rest state of spin level. The temperature effect on the structure change was great at the UL in the equatorial reflection. But those of IASL and MSL were minor. Equatorial reflection (10, 11) change inferred that myosin head was moved to the vicinity of actin filament by temperature change (from $25^{\circ}C$ to $0^{\circ}C$) at UL, but spin level was not changed. The intensity change of 143 $\AA$ and 72 $\AA$ could offer information of the mass profection of population of myosin heads along the filament axis. The slope of intensity profile of the mass profection of 143$\AA$ and reflection of MSL is appeared sharply and those of UL and IASL were not changed. The decrease of MSL actin reflection at 51 $\AA$ and 59 $\AA$ in the actin reflection change refers that the shifted myosin head binds a certain actin or changes an actin structure. From these results, we could conclude that IASL and MSL were spin labeled on SH of myosin head and disordered the helix arrangement of actin.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제49권2호
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• pp.87-91
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• 2000

Metal ion complex of poly(N-(2,4-imidazoly)ethyl)maleimide-alt-l-octadecene (IMO-O) polymer used to confirm the possibility of molecular device made by Langmuir-Blodgett(LB) method. Electrical properties of the metal ion complex LB film were investigated using Metal/Insulator/Metal(MIM) structure. In the surface pressure-area($\pi$-A) isotherm of IMI-O polymer, the surface pressure at collapse point has a difference due to the interaction between polymer and metal ions. And the complex between polymer and metal ions could be verified through the investigation by Raman spectroscopy. In the current-voltage(I-V) property, the conductivity change of IMO-O polymer complexes due to the kinds of metal of metal ions couldn't be observed. However, the limiting area of molecules was changed by the concentration of the metal ions and the conductivity was increased with the occupied molecular area.

• 약학회지
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• 제41권1호
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• pp.74-80
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• 1997

A polygonal antibody against recombinant hirudin was raised for the development of a ELISA in biological fluids. Recombinant hirudin was conjugated to maleimide activated carrie r protein, KLH and injected to a rabbit. The third booster collection of antiserum was used as primary antibody for the ELISA. The titer for the detection antibody was determined. The direct ELISA could determine the concentration of hirudin in the range of ~10ng/ml. Affinity pulified IgG was obtained and conjugated to horseradish peroxidase. Purified IgG and IgG-HRP could be used as capture and detection antibody, respectively. Although sandwich ELISA would not give the satisfactory results. it could apply for the detection of hirudin level in the range of ~20 ${\mu}$g/ml.

• 공업화학
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• 제31권2호
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• pp.226-229
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• 2020

ABS 수지의 내열성 강화를 위한 화합물인 N-phenylmaleimide (PMI)는 원료로서 노란색을 띤다. 그러므로 PMI로 개질된 공중합체는 색을 나타내는데, 이에 따른 사용의 제한이 있다. 이러한 단점을 극복하기 위해 PMI의 대체재로, 원료로서 색을 띠지 않고 PMI와 비슷한 성능을 가진 N-cyclohexylmaleimide (CHMI)의 수요 또한 증가하고 있다. 그러나 CHMI는 합성 중에 여러 반응 부산물이 생성되어 정제 과정이 필수적이며, 수율도 낮아 공업화에 어려움이 있다. 본 연구에서는 이러한 문제점을 개선하고 CHMI를 공업적으로 생산하기 위해 복합 촉매를 개발하였다. 최종적으로 CHMI를 85% 수율과 99.5% 이상의 순도로 합성하였다.

• 한국응용과학기술학회지
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• 제22권1호
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• pp.84-90
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• 2005

IASL(iodo acetamide) and MSL(maleimide) disordered the orderly helix arrangement of myosin in the rest state of spin level. Especially the effect of IASL was great. Equatorial refiection(10,11) change inferred that myosin head was moved to the vicinity of actin filament by spin level. The intensity change of 143${\AA}$ and 72${\AA}$ could offer information of the mass projection of population of myosin heads along the :filament axis. The slope of intensity profile of the mass projection of 143${\AA}$ and reflection of IASL is appeared and that of MSL is appeared sharply. The decrease of 215${\AA}$ reflection intensity is appeared the periodical characteristic of 143${\AA}$ reflection by spin label. The raise of MSL actin reflection at 51${\AA}$ and 59${\AA}$ in the actin reflection change refers that the shifted myosin head binds a certain actin or changes an actin structure by spin label effect. Because iodo acetamide has a tendency to decease the actin reflection, actin dose not bind myosin head. From this result, we could conclude that LASL and MSL are spin labeled on SH of myosin head and disordered the helix arrangement of actin.

• 한국응용과학기술학회지
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• 제20권3호
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• pp.268-273
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• 2003

IASL(iodo acetamide spin label) and MSL(maleimide spin label) disordered the orderly helix arrangement of myosin in the rest state of spin level. Especially the effect of IASL was great. The muscle was isometrically tetanized with three trains of 3ms pulses every 50ms between $5^{\circ}C$ with $25^{\circ}C$. Equatorial reflection change inferred that myosin head was moved to the vicinity of actin filament by spin level. The intensity change of $143{\AA}$ and $72{\AA}$ could offer information of the mass projection of population of myosin head along the filament axis. The slope of intensity profile of the mass projection of $143{\AA}$ and reflection of IASL is appeared and that of MSL is appeared sharply. The decrease of $215{\AA}$ reflection intensity the periodical character of $143{\AA}$ reflection by spin label. The raise of MSL actin reflection at $51{\AA}$ and $59{\AA}$ in the actin reflection change refers that the shifted myosin head binds a certain actin or changes an actin structure by spin label effect. Because iodo acetamide has a tendency to decease the actin reflection, actin dose not bind myosin head. From this result, we can conclude that IASL and MSL are spin labeled on SH of myosin head and disordered the helix arrangement of actin.

• 생명과학회지
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• 제8권6호
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• pp.681-686
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• 1998

이상의 결과로부터 spin lavel의 영향은 IASL, MSL은 이완상태 myosin head의 규칙적인 나선 배열은 흐트러진다. 특히 IASL은 효과가 크다. 적도반사의 변화로부터 myosin head는 spin label하는 것에 의해 actin filament 근방에 이동해 있다는 것을 알 수 있었다. 215 $\AA$ 반사의 감소는 spin lavel에 의한 myosin의 143 $\AA$주기성을 더욱 강하게 해 준다. 143 $\AA$, 72 $\AA$의 거동은 filament축으로부터 투영한 구조를 반영하기 때문에 IASL에는 143 $\AA$ 주기의 밀도분포가 완만하게 되어 있다는 것을 나타내고, MSL에는 143 $\AA$ 분포가 올라와 있다는 것을 나타낸다. actin 반사변화에서 MSL의 actin반사의 증가는 이동한 myosin head가 어떤 actin과 결합하였거나, spin lavel의 영향으로 actin의 구조가 변화되었다. 한편, IASL은 actin반사를 감소 시키기 때문에 myosin head의 결합을 하지 않았다. 결론적으로, 이것은 actin의 SH기에도 spin label되어 actin의 나선 구조가 크게 흐트러짐을 알 수 있었다