• Journal of the Korean Magnetic Resonance Society
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• v.21 no.3
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• pp.96-101
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• 2017

Metabolomics is one of latest '-omics', which is to analyze metabolome in cells, tissues and biofluids and to study metabolisms. It has become increasingly popular since 1990. The first goal of metabolomics is to analyze metabolites in a technical aspect. The major two analytical platforms in metabolomics are NMR spectroscopy and mass spectrometry (MS). MS is superior to NMR for detecting many more metabolites. That is one of the most important factors in metabolomics. However, NMR also has several advantages over MS. In this review, I firstly introduced metabolomics by comparing NMR-based metabolomics and MS-based metabolomics. Second, I explored technical issues on sample preparation and NMR experiments for metabolite identification and quantification.

• Journal of the Korean Magnetic Resonance Society
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• v.17 no.1
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• pp.1-10
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• 2013

Metabolomics is the study which detects the changes of metabolites level. Metabolomics is a terminal view of the biological system. The end products of the metabolism, metabolites, reflect the responses to external environment. Therefore metabolomics gives the additional information about understanding the metabolic pathways. These metabolites can be used as biomarkers that indicate the disease or external stresses such as exposure to toxicant. Many kinds of biological samples are used in metabolomics, for example, cell, tissue, and bio fluids. NMR spectroscopy is one of the tools of metabolomics. NMR data are analyzed by multivariate statistical analysis and target profiling technique. Recently, NMR-based metabolomics is a growing field in various studies such as disease diagnosis, forensic science, and toxicity assessment.

• Korean Journal of Acupuncture
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• v.25 no.4
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• pp.147-166
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• 2008

Objective : This study is achieved to know the way that we can use metabolomics for the scientific research of Korean medicine. Results : We elucidate that metabolomics can be contributed to four ways for objectification of Korean medicine 1. Diagnosis of BeunJungLonChi(辯證論治). 2. Application toward Personalized medicine. 3. Quantification of Herbal medicine. 4. Evaluation of efficacy/safety for Korean medicine formulation Conclusion : Metabolomics opens up a new opportunity to reinvestigate Korean medicine from holistic perspective. Furthermore, Metabolomics is expected to provide effective method in research of acupuncture and meridian.

• KSBB Journal
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• v.24 no.2
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• pp.113-121
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• 2009

As one of the new areas of 'omics' technology, there is increasing interest in metabolomics, which involves the analysis of low-molecular-weight compounds in cells, tissues, and biofluids, and considers interactions within various organisms and reactions of external chemicals with those organisms. However, metabolomics research is still at a fundamental stage in Korea. Therefore, the purpose of this study was to establish a strategic long-term plan to revitalize the national metabolomics approach and obtain the elementary data necessary to determine a policy for effectively supporting metabolomics research. These investigations clarified the state of metabolomics study both in Korea and internationally, from which we attempted to find the potentiality and fields where a metabolomics approach would be applicable, such as in medical science. We also discuss strategies for developing metabolomics research. This study revealed that promoting metabolomics in Korea requires cooperation with metabolomics researchers, acquisition of advanced technology, capital investment in metabolomics approach, establishment of metabolome database, and education of metabolome analysis experts. This would reduce the gap between the national and international levels of metabolomics research, with the resulting developments in metabolomics having the potential to greatly contribute to promoting biotechnology in Korea.

• Toxicological Research
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• v.25 no.2
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• pp.59-69
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• 2009

Metabolomics which deals with the biological metabolite profile produced in the body and its relation to disease state is a relatively recent research area for drug discovery and biological sciences including toxicology and pharmacology. Metabolomics, based on analytical method and multivariate analysis, has been considered a promising technology because of its advantage over other toxicogenomic and toxicoproteomic approaches. The application of metabolomics includes the development of biomarkers associated with the pathogenesis of various diseases, alternative toxicity tests, high-throughput screening (HTS), and risk assessment, allowing the simultaneous acquisition of multiple biochemical parameters in biological samples. The metabolic profile of urine, in particular, often shows changes in response to exposure to xenobiotics or disease-induced stress, because of the biological system's attempt to maintain homeostasis. In this review, we focus on the most recent advances and applications of metabolomics in toxicological research.

• Animal Bioscience
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• v.36 no.2_spc
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• pp.374-384
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• 2023

Skeletal muscle metabolism regulates homeostatic balance in animals. The metabolic impact persists even after farm animal skeletal muscle is converted to edible meat through postmortem rigor mortis and aging. Muscle metabolites resulting from animal growth and postmortem storage have a significant impact on meat quality, including flavor and color. Metabolomics studies of postmortem muscle aging have identified metabolisms that contain signatures inherent to muscle properties and the altered metabolites by physiological adaptation, with glycolysis as the pivotal metabolism in postmortem aging. Metabolomics has also played a role in mining relevant postmortem metabolisms and pathways, such as the citrate cycle and mitochondrial metabolism. This leads to a deeper understanding of the mechanisms underlying the generation of key compounds that are associated with meat quality. Genetic background, feeding strategy, and muscle type primarily determine skeletal muscle properties in live animals and affect post-mortem muscle metabolism. With comprehensive metabolite detection, metabolomics is also beneficial for exploring biomarker candidates that could be useful to monitor meat production and predict the quality traits. The present review focuses on advances in farm animal muscle metabolomics, especially postmortem muscle metabolism associated with genetic factors and muscle type.

• Journal of the Korean Magnetic Resonance Society
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• v.22 no.1
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• pp.10-17
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• 2018

Metabolite identification and quantification are one of the foremost important issues in metabolomics. In NMR based metabolomics, mainly one-dimensional proton NMR spectra of biofluids, such as urine and serum are measured. However, it is not always easy to identify and quantify metabolites in one-dimensional proton NMR spectra. This article introduces useful public metabolite databases, metabolic profiling software, and articles.

• Molecules and Cells
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• v.38 no.7
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• pp.587-596
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• 2015

Obesity and diabetes arise from an intricate interplay between both genetic and environmental factors. It is well recognized that obesity plays an important role in the development of insulin resistance and diabetes. Yet, the exact mechanism of the connection between obesity and diabetes is still not completely understood. Metabolomics is an analytical approach that aims to detect and quantify small metabolites. Recently, there has been an increased interest in the application of metabolomics to the identification of disease biomarkers, with a number of well-known biomarkers identified. Metabolomics is a potent approach to unravel the intricate relationships between metabolism, obesity and progression to diabetes and, at the same time, has potential as a clinical tool for risk evaluation and monitoring of disease. Moreover, metabolomics applications have revealed alterations in the levels of metabolites related to obesity-associated diabetes. This review focuses on the part that metabolomics has played in elucidating the roles of metabolites in the regulation of systemic metabolism relevant to obesity and diabetes. It also explains the possible metabolic relation and association between the two diseases. The metabolites with altered profiles in individual disorders and those that are specifically and similarly altered in both disorders are classified, categorized and summarized.

• Journal of Plant Biotechnology
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• v.37 no.1
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• pp.12-24
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• 2010

Plant metabolomics is a research field for identifying all of the metabolites found in a certain plant cell, tissue, organ, or whole plant in a given time and conditions and for studying changes in metabolic profiling as time goes or conditions change. Metabolomics is one of the most recently developed omics for holistic approach to biology and is a kind of systems biology. Metabolomics or metabolite fingerprinting techniques usually involves collecting spectra of crude solvent extracts without purification and separation of pure compounds or not in standardized conditions. Therefore, that requires a high degree of reproducibility, which can be achieved by using a standardized method for sample preparation and data acquisition and analysis. In plant biology, metabolomics is applied for various research fields including rapid discrimination between plant species, cultivar and GM plants, metabolic evaluation of commercial food stocks and medicinal herbs, understanding various physiological, stress responses, and determination of gene functions. Recently, plant metabolomics is applied for characterization of gene function often in combination with transcriptomics by analyzing tagged mutants of the model plants of Arabidopsis and rice. The use of plant metabolomics combined by transcriptomics in functional genomics will be the challenge for the coming year. This review paper attempted to introduce current status and prospects of plant metabolomics research.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.2
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• pp.179-186
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• 2014

Metabolomics is the study of changes in the metabolic status of an organism as a consequence of drug treatment, environmental influences, nutrition, lifestyle, genetic variations, toxic exposure, disease, stress, etc, through global or comprehensive identification and quantification of every single metabolite in a biological system. Since most chronic diseases have been demonstrated to be linked to nutrition, nutritional metabolomics has great potential for improving our understanding of the relationship between disease and nutritional status, nutrient, or diet intake by exploring the metabolic effects of a specific food challenge in a more global manner, and improving individual health. In particular, metabolite profiling of biofluids, such as blood, urine, or feces, together with multivariate statistical analysis provides an effective strategy for monitoring human metabolic responses to dietary interventions and lifestyle habits. Therefore, studies of nutritional metabolomics have recently been performed to investigate nutrition-related metabolic pathways and biomarkers, along with their interactions with several diseases, based on animal-, individual-, and population-based criteria with the goal of achieving personalized health care in the future. This article introduces analytical technologies and their application to determination of nutritional phenotypes and nutrition-related diseases in nutritional metabolomics.