• Title, Summary, Keyword: monounsaturated fatty acid

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Investigation of cultivation and FAME composition isolated Phaeodactylum tricornutum from Youngheung island (영흥도에서 분리된 Phaeodactylum tricornutum의 증식 및 Monounsaturated fatty acid 관련 지방산 조성 분석)

  • Lee, SangMin;Cho, Yonghee;Shin, ong-Woo;Jeon, Hyonam;Ryu, YoungJin;Lim, Sang-Min;Lee, Choul-Gyun
    • Journal of Marine Bioscience and Biotechnology
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    • v.6 no.2
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    • pp.47-52
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    • 2014
  • Oxidation stability and cold fuid property are considered as the most important factors for determining biodiesel quality. Among the fatty acids, monounsaturated fatty acid satisfy both oxidation stability and cold flow property of biodiesel quality standards. Microalgae with high monounsaturated fatty acid contents is have some benefit for producing to produce biodiesels with satisfying quality standards. In this study, monounsaturated fatty acid contents of a isolated microalga from Youngheung island was analyzed. Phaeodactylum tricornutum was isolated by streaking, and growth rate and fatty acid composition of the algae were investigated. Total FAME contents were consisted of 26% of saturated fatty acids, 43% of monounsaturated fatty acids, and 18% of polyunsaturated fatty acids. The contents of monounsaturated fatty acid were especially high in the Phaeodactylum species. This result implies that the FAMEs from P. tricornutum may contribute to improve the oxidation stability and cold flow property of biodiesel.

Conjugated Linoleic Acid Changes fatty Acid Composition by Decreasing Monounsaturated fatty Acids in Rabbits and Hep G2 Cells

  • Nam, Kisun
    • Journal of Nutrition and Health
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    • v.30 no.4
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    • pp.442-450
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    • 1997
  • Conjugated dienoic derivatives of linoleic acid(CLA) are a mixture of positional and geometric isomers of linoleic acid(LA). We previously found that CLA changes the fatty acid profile in chicken eggs and serum by decreasing monounsaturated fatty acids. Studies were conducted to explore the effects of CLA on fatty acid composition. Rabbits were fed a semisynthetic diet with or without CLA(0.5g CLA/rabbit/day) for 22 weeks. Compared to the control, rabbits fed CLA had significantly lower monounsaturated fatty acid levels(palmitoleic acid Cl6 : 1 by 50% and oleic acid Cl8 : 1, by 20%) in plasma lipids. We found similar differences in fatty acid composition in the liver and the aorta. The inhibitory effect of CLA on $\Delta$9 desaturation was confirmed in a human hepatoma cell line, Hep G2. CLA significantly decreased $\Delta$9 desaturation in 4-5 hours as shown by an increase in the ratio of Cl6 : 0 to C 16 1, This is apparently due to a decrease in $\Delta$9 desaturase(stearoyl-CoA desaturase, SCD) activity ; it was decreased more than 50%. These results, along with our previous findings, indicate that CLA is an inhibitor of $\Delta$9 desaturase in the liver.

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The Change in Content of Constitutive Lipid and Fatty Acid of Pacific Saury during Natural Freezing Dry(Kwa Mae Kee) (꽁치 자연동결건조(과메기)중 지방함량과 지방산 조성 변화)

  • 오승희;김덕진
    • The Korean Journal of Food And Nutrition
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    • v.8 no.3
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    • pp.239-252
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    • 1995
  • The results which were examined changes In contents of constitutive lipid and fatty acid during the natural drying of Pacific Saury were as follows. During the natural drying of Pacific Saury, offal lipid and moisture contents gradually decreased. In the survey of rancidity degree extracted lipid of Pacific Saury, peroxide value was gradually Increased, TBA and acid value were radically increased thereafter 9 days of drying. Neutral lipid, phospholipid and glycolipid contents of total lipid were 95%, 4% and 2%, respectively, and in the neutral lipid, content of TG was more than 90%, and in the phospholipid, contents of PC and PE were 45∼85%, 27∼34%, respectively. Fatty acid composition of total lipid was substantially higher in C18:0 and C20:1, C22:1, C22:6 contents and fatty acid composition of neutral lipid was higher In C22:1, C22:1, contents and fatty acid composition of phospholipid was the highest in C22:6 content. In the fatty acid composition of glycolipid, monounsaturated fatty acid content has substantially higher, C16:0 content was gradually Increased In saturated fatty acid and C22:1 content was gradually decreased in monounsaturated fatty acid. It showed that fatty acid composition of triglyceride has higher in monounsaturated fatty acid content and contents of C20:1, C22:1 was gradually decreased.

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Association of -867G>C, -877Gdel, and Exon 5G>T Polymorphisms in the Stearoyl-CoA Desaturase (SCD) Gene with Fatty Acid Composition in the M. longissimus dorsi Muscle of Hanwoo (Korean Cattle)

  • Cho, Yong-Min;Lee, Seung-Hwan;Park, Eung-Woo;Kim, Nam-Kuk;Lim, Da-Jeong;Kim, Kyoung-Hoon;Park, Beom-Young;Lee, Chang-Soo;Oh, Sung-Jong;Kim, Tae-Hun;Yoon, Du-Hak
    • Food Science of Animal Resources
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    • v.30 no.4
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    • pp.655-660
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    • 2010
  • This study aimed to identify genetic polymorphisms associated with fatty acid composition in Hanwoo beef. In this study, three SNPs (-867G>C, -877Gdel and 878T>C) were detected in SCD gene by DNA sequencing and PCR-RFLP. Statistical analysis revealed that 878T>C SNP was significantly associated with total saturated (p=0.016), unsaturated (p=0.016), and monounsaturated fatty acid (p=0.026) composition. However, the other two SNPs (-867G>C and -877Gdel) that are detected in the regulatory region of the SCD gene have no association with the fatty acid composition of Hanwoo meat. The 878C (alanine type) allele was found to be associated with 2.2% higher monounsaturated fatty acid, 1.5% lower saturated fatty acid, and 1.4% higher unsaturated fatty acid content than those associated with the 878T (valine type) allele. These results indicate that the non-synonymous SNP (878T>C) in the SCD gene could be a causal mutation that contributes to the MUFA variation in Hanwoo beef.

Monounsaturated Fatty Acids와 혈중 Cholesterol

  • 지석우;지규만
    • Proceedings of the Korea Society of Poultry Science Conference
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    • pp.14-35
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    • 2000
  • There have been growing interests in olive oil, which is attributed to the beneficial health effects of the Mediterranean diet. Numerous studies suggest that oleic acid, the major fatty acid in olive oil, may contribute to less incidence of coronary heart disease(CHD) in the Mediterranean area. Although the major effects of high monounsaturated fatty acid(MUFA) consumption on lowered plasma cholesterol are generally attributed to the consequential replacement of saturated fatty acid in diets, some studies have shown a modest cholesterol lowering effect of MUFA alone when it replaces dietary carbohydrate at an equal amount of energy. Furthermore, MUFA increases the level of the protective high-density lipoprotein cholesterol more than polyunsaturated fatty acid(PUFA) does when these two classes of fatty acids replace carbohydrates in the diet. It has been generally recommended that optimal dietary fat should be rich in MUFA, such as oleic acid, low in saturated fatty acids and provide adequate amounts of PUFA. However, no conclusive observations have been made yet mainly due to a difficulty in controlling the proportion of oleic acid without adjusting the levels of the other fatty acids to be constant, which could also affect plasma cholesterol levels. Recent findings also suggest that dietary PUFA results in higher tendency toward lipid peroxidation whereas with MUFA the susceptibility of low density lipoprotein to oxidative stress is reduced. More studies are needed to clarify the effects of MUFA on reducing the incidence of CHD.

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Membrane Lipids of a Marine Ciliate Protozoan Uronema marinum

  • Seo Jung Soo;Kim Ki Hong;Lee Hyung Ho;Chung Joon Ki
    • Fisheries and aquatic sciences
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    • v.6 no.3
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    • pp.155-159
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    • 2003
  • Lipid composition and fatty acid composition were characterized in the membrane of a marine ciliate protozoan (Uronema marinum). Phospholipids accounted for 70% of total lipid, and the remainder was neutral lipids. Total phospholipids were separated as phosphatidylcholine $(24.26\%)$, phosphatidylethanolamine $(22.21\%)$, phosphatidylinositol $(6.14\%)$, phosphatidyl­serne $(5.11\%)$, cardiolipin $(3.07\%)$ and unidentified phospholipids $(28.72\%)$ through high performance liquid chromatography (HPLC). Fatty acid composition of neutral lipids and phospholipids was determined by gas chromatography (GC), based solely on comparision of retention times. In neutral lipids, the most abundant fatty acid group was monounsaturated fatty acid $(48.3\% of total fatty acids)$ with oleic acid (18:1) and nervonic acid (24:1). Saturated fatty acids comprised $29.6\%$ of total fatty acids, with palmitic acid (16:0), stearic acid (18:0) ane myristic acid (14:0), and polyunsaturated fatty acid accounted for $33.0\%$ with $Di-homo-\gamma-linolenic$ acid (20:3) and linoleic acid (18:2). Wherease phospholipids predominantly contained the fatty acid group in the following order: polyunsaturated fatty acids $(52.7\%\;of\;total\;fatty\;acids)$ with linoleic acid (18:2) and $\gamma-linolenic$ acid (18:3) > monounsaturated fatty acids $(28.5\%\;of\;total\;fatty\;acids)$ with oleic acid (18:1) and palmitoleic acid (16:1) > saturated fatty acids $(25.5\%\;of\;total\;fatty\;acids)$ with palmitic acid (16:0), stearic acid (18:0) and myristic acid (14:0).

Fatty Acid Compositions of Lipids Extracted from Bullfrogs (황소개구리에서 추출한 지방의 지방산 조성)

  • 황금택;홍진선;강성국;정순택
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.2
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    • pp.351-354
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    • 2002
  • The objective of this study was to analyze fatty acid composition in lipids extracted from bullfrogs (Rana catesbeiana Shaw) inhabiting in Korea. Lipid contents in bullfrog legs and bodies were less than 1% (w/w, wet basis) and seasonal variation of the lipid contents was not observed. Lipids in bullfrog legs consisted of 26~31% (w/w) saturated fatty acids, 16~24% monounsaturated fatty acids, and 30~40% polyunsaturated fatty acids. Lipids in bullfrog bodies consisted of 23~28% saturated fatty acids, 29~44% monounsaturated fatty acids, and 16~30% polyunsaturated fatty acids. The major fatty acids in lipids extracted from bullfrogs were palmitic acid, oleic acid, and linoleic acid. Lipids in leg muscles contained 3~8% eicosapentaenoic acid (EPA) and 6~10% docosahexaenoic acid (DHA). Lipids in bodies had 1~3% EPA and 1~3% DHA.

Morphological, Molecular, and Biochemical Characterization of Monounsaturated Fatty Acids-Rich Chlamydomonas sp. KIOST-1 Isolated from Korea

  • Jeon, Seon-Mi;Kim, Ji Hyung;Kim, Taeho;Park, Areumi;Ko, Ah-Ra;Ju, Se-Jong;Heo, Soo-Jin;Oh, Chulhong;Affan, Md. Abu;Shim, Won-Bo;Kang, Do-Hyung
    • Journal of Microbiology and Biotechnology
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    • v.25 no.5
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    • pp.723-731
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    • 2015
  • Microalgae hold promise as producers of sustainable biomass for the production of biofuels and other biomaterials. However, the selection of strains with efficient and robust production of desirable resources remains challenging. In this study, we isolated a green microalga from Korea and analyzed its morphological, molecular, and biochemical characteristics. Microscopic and phylogenetic analyses demonstrated that the isolate could be classified into the genus Chlamydomonas, and we designated the isolate Chlamydomonas sp. KIOST -1. Compositions of protein, lipid, and carbohydrate in the microalgal cells were estimated to be 58.8 ± 0.2%, 22.7 ± 1.2%, and 18.5 ± 1.0%, respectively. Similar to other microalgae belonging to Chlorophyceae, the dominant amino acid and monosaccharide in Chlamydomonas sp. KIOST-1 were glutamic acid and glucose. On the other hand, the proportions of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids clearly differed from other species in the genus Chlamydomonas, and monounsaturated fatty acids accounted for a large portion (41.3%) of the total fatty acids in the isolate. Based on these results, Chlamydomonas sp. KIOST-1 has advantageous characteristics for biomass production.

Studies on Fatty Acids Composition of Different Portions in Various Meat (식육의 종류 및 부위에 따른 지방산 조성에 관한 연구)

  • 신기간;박형일;이성기;김천제
    • Food Science of Animal Resources
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    • v.18 no.3
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    • pp.261-268
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    • 1998
  • In beef, the composition of saturated and unsaturated fatty acids(UFA) were 41.1% and 56.7%, respectively. The rates of saturated fatty acid (SFA) was 36.3, 40.9, 39.2 and 48.1% in loin, picnic shoulder, ham and rib, respectively. However, UFA showed 62.3, 58.4, 56.8, 50.9% in loin, picnic shoulder, ham and rib, respectively. The rates of monounsaturated fatty acid (MUFA) were similar to those of unsaturated fatty acid in four portions. The content of SFA, UFA and polyunsaturated fatty acid(PUFA) were 40, 59 and 9.4%, respectively, in pork. In portional comparison, composition of unsaturated fatty acids in picnic shoulder, ham rib and loin were 64.2, 60.4, 54.6 and 55.3%, respectively. The UFA contents of rib and loin were lower than picnic shoulder and ham, but SFA levels were higher. Total UFA content of chicken was 62.9% showing higher level than other species, but low when compared to that of duck meat. SFA content resulted in lower level than other portions. In the skin of chicken, MUFA content was especially high showing no significant changes in UFA contents. Duck meat showed lower SFA content, but higher content in UFA compared to those of other species. Skin and thigh contained 31.8 and 31.5%, respectively, in SFA, which are lower level than those of breast and thigh meat. The UFA contents of skin and thigh were 67.4 and 67.7%, respectively, which are relatively high compared to those of other portions. Meanwhile, the ratio of UFA was not high in canis meat compared to other meat. PUFA was, however, 24.2% showed significantly different from other meats. The contents of SFA were 35.4% and 35.9%, respectively, in picnic shoulder and ham. They showed lower SFA but higher UFA content than those of rib and loin. In contrast, the ratio of picnic shoulder and ham were 61.6 and 61.4%, respectively. Interestingly, picnic shoulder showed higher UFA content, especially in arachidonic acid level than other portions.

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Comparisons of Feeding Ecology of Euphausia pacifica from Korean Waters Using Lipid Composition (한국 근해의 난바다곤쟁이 Euphausia pacifica의 지방 조성에 의한 섭식 생태 비교)

  • Kim, Hye-Seon;Ju, Se-Jong;Ko, Ah-Ra
    • Ocean and Polar Research
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    • v.32 no.2
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    • pp.165-175
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    • 2010
  • Dietary lipid biomarkers (fatty acids, fatty alcohols, and sterols) in adult specimens were analyzed to compare and understand the feeding ecology of the euphausiid, Euphausia pacifica, from three geographically and environmentally diverse Korean waters (Yellow Sea, East China Sea, and East Sea). Total lipid content of E. pacifica from Korean waters was about 10% dry weight (DW) with a dominance of phospholipids (>46.9% of total lipid content), which are known as membrane components. A saturated fatty acid, C16:0, a monounsaturated fatty acid, C18:1(n-9), and two polyunsaturated fatty acids, C20:5(n-3) and 22:6(n-3), were most abundant (>60% of total fatty acids) in the fatty acid composition. Some of the fatty acids showed slight differences among regions although no significant compositional changes of fatty acids were detected between these regions. Phytol, originating from the side chain of chlorophyll and indicative of active feeding on phytoplankton, was detected all samples. Trace amounts of various fatty alcohols were also detected in E. pacifica. Specifically, krill from the Yellow Sea showed relatively high amounts of longchain monounsaturated fatty alcohols (i.e. 20:1 and 22:1), generally found in herbivorous copepods. Three different kinds of sterols were detected in E. pacifica. The most dominant of these sterols was cholest-5-en-$3{\beta}$-ol (cholesterol). The lipid compositions and ratios of fatty acid trophic markers are indicative of herbivory in E. pacifica from the Yellow Sea and East Sea (mainly feeding on dinoflagellates and diatoms, respectively). The lipid compositions and ratios of fatty acid trophic markers are indicative of carnivory or omnivory in E. pacifica from the East China Sea, mainly feeding on microzooplankton such as protozoa. In conclusion, lipid biomarkers provide useful information about krill feeding type. However, further analyses and experiments (i.e. gut content analysis, in situ grazing experiment, etc.) are needed to better understand the feeding ecology of E. pacifica in various marine environments.