• Title/Summary/Keyword: morphological observation

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Morphological and Anatomical Evaluation of Grafted Pinus merkusii

  • Susilowati, Arida;Iswanto, Apri Heri;Wahyudi, Imam;Supriyanto, Supriyanto;Siregar, Iskandar Z
    • Journal of the Korean Wood Science and Technology
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    • v.44 no.6
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    • pp.903-912
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    • 2016
  • Morphological and anatomical evaluation of grafted P. merkusii have been undertaken to obtain the information about compatible and incompatible symptoms of 18 years old grafts based on morphological observation and microscopic analysis. Samples of compatible and incompatible grafts were obtained from previous research conducted by the Silviculture Departement Team in 1994. Result showed that compatible grafts have normal stem form and secondary growth (diameter growth), but some abnormality symptoms like undulated pattern of annual growth rings, phloem thickening and abnormality resin ducts in inner and middle parts of the union area occurred. Incompatible ones showed abnormality of the stem form, cortex-bark necrosis and swelling in the union area. Microscopic observation showed abnormality of all parts of the union, undulated pattern of annual growth rings, phloem thickening, abnormal resin ducts, low numbers and discontinuity of vascular elements in the union area.

Pharmacognostical Studies on Alisma Plants (택사의 생약학적 연구)

  • Toh, Chung-Ae
    • Korean Journal of Pharmacognosy
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    • v.26 no.4
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    • pp.411-418
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    • 1995
  • In order to clarify the originality of several Alisma Rhizomes, we investigated the differences of their morphological, anatomical and physiochemical characteristics. Morphological observation shows the variability of Alisma shape and quality. The diversities in morphological shapes and qualities of tuber were observed among different origins. Chinese product looked like corm shape, but korean's irregular shape. The microscopic observation of korean Alisma revealed the presence of irregular vascular bundle which was scattered transversely and longitudinaly, which was different from that of the rhizome. This facts suggest that the portion of crude drug in Alisma should be the corm rather than the rhizome. In the physicochemical differences, chinese Alisma contains larger amount of mineral elements such as K, Na and Ca than korean Alisma. On the contrary, protein contents in lyophilized powder of aqueous extracts in korean Alisma rhizome was significantly higher than chinese Alisma rhizome. The leaves and stems contained larger amount of Ca and Na than tuber, and may be used as the osmotic diuretics for chinese traditional medicine.

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Use of Unopette for the Observation of Sperm Morphology and Sperm Concentration (정자형태 및 정자농도의 검사를 위한 Unopette의 사용)

  • Kim Myung-Cheol
    • Journal of Veterinary Clinics
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    • v.7 no.2
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    • pp.497-500
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    • 1990
  • This study was carried out determine whether Unopette can be used for the observation of sperm morphology and sperm Concentration. Rabbit sperm and frozen-thawed bovine sperm were observed with phase contrast microscope after dilution with Unopette acooriding to duration of preservation at 3~5$^{\circ}C$. Sperm using Unopette showed high normal sperm(%) than sperm using hematoxylin-eosin until 48 hours. Sperm using Unopette revealed no difference in sperm concentration until 24 hours, as compared with control sperm. As a result, Unopette was assessed as appropriate solution for preservation in terms of morphological observation and sperm concentration.

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A Study on a Morphological Identification of Adenophora triphylla var. japonica, Codonopsis lanceolata, Adenophora remotiflora and Codonopsis pilosula (사삼(沙參), 양유근(羊乳根), 제니 및 당삼(黨參)의 형태(形態) 감별에 관한 연구)

  • Kim, Joo-Young;Lee, Young-Jong
    • The Korea Journal of Herbology
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    • v.22 no.2
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    • pp.121-126
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    • 2007
  • Objectives : A morphological classification among Adenophora triphylla var. japonica, Codonopsis lanceolata, Adenophora remotiflora and Codonopsis pilosula was made through microscopic observation. Method : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Result : 1. Codonopsis pilosula is like a long cone, with amole-like projected parts at the beginning of the roots. 2. Adenophora triphylla var. japonica, Codonopsis lanceolata, Adenophora remotiflora, and Codonopsis pilosula are all similar as following: 1) Cork layers are composed of a few to over 10 subsidiary cells, with individual or a group of stone cells among them. 2) Phloem is wide and has a milk tube. Conclusion : Adenophora triphylla var. japonica, Codonopsis lanceolata, Adenophora remotiflora, and Codonopsis pilosula are all similar and difficult to be distinguished through microscopic observation, so that chemical experiments should be conducted for their identification.

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Disinfection by Ozone Microbubbles Can Cause Morphological Change of Fusarium oxysporum f. sp. melonis Spores

  • Tamaki, Masahiko;Kobayashi, Fumiyuki;Ikeura, Hiromi;Sato, Michio
    • The Plant Pathology Journal
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    • v.34 no.4
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    • pp.335-340
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    • 2018
  • To investigate the difference in the disinfectant efficiency of ozone microbubbles ($O_3MB$) and ozone millibubbles ($O_3MMB$), the morphological change of the treated Fusarium oxysporum f. sp. melonis spores was observed with scanning and transmission electron microscopies (SEM and TEM). The disinfectant efficiency of $O_3MB$ on F. oxysporum f. sp. melonis spores was greater than that of $O_3MMB$. On observation with SEM, it was revealed that morphological change of F. oxysporum f. sp. melonis spores was caused by $O_3MB$ and $O_3MMB$, and damage to the spore surfaces by $O_3MB$ occurred sooner than that by $O_3MMB$. On observation with TEM, it was furthermore confirmed that F. oxysporum f. sp. melonis spores treated with $O_3MB$ induced wavy deformation of cell membrane and the intracellular change different from that with $O_3MMB$. Therefore, the greater disinfection efficiency of $O_3MB$ was suggested to be caused due to the function of the MB in addition to the oxidative power of $O_3$.

Morphological Observation and Silicate Content of Short Culm Mutants in Rice (수도두간변이계통의 형태 및 규산함량에 관한 연구)

  • 한창열
    • Journal of Plant Biology
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    • v.14 no.3
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    • pp.29-35
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    • 1971
  • Various types of short culm rice mutants were obtained by means of gamma-ray irradiation. In the present paper morphological observation and analysis of silicate content of the mutants are reported. 1. Short culm type had more useful characters than bushy and dwarf types. 2. In short culm and bushy types the number of nodes both above and under ground was similar to the mother varieties, while in dwarf type it decreased. 3. In short culm types the variation of length of above-ground culm and internodes tended to vary relattive to the mother varieties. 4. Positive correlation was found between culm length and the first and fourth internode length in short culm type. 5. Silicate content increased in short culm type-the increment being variety specific.

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Effect of AL072, a Novel Anti-Legionella Antibiotic, on Growth and Cell Morphology of Legionella pneumophila

  • Kang, Byeong-Cheol;Park, Jae-Hak;Lee, Yong-Soon;Suh, Jung-Woo;Chang, Jun-Hwan;Lee, Chul-Hoon
    • Journal of Microbiology and Biotechnology
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    • v.9 no.3
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    • pp.371-375
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    • 1999
  • AL072 is a potent anti-Legionella antibiotic produced by Streptomyces strain AL91. The minimum inhibitory concentration (MIC) of AL072 against Legionella pneumophila was 0.2$\mu$g/ml. Bacterial growth was rapidly inhibited at the dose range between the MIC and 20 times of the MIC when the antibiotic was added at the mid-exponential phase. Ultrastructural changes in L. pneumophila were observed upon treatment with AL072. Under electron microscopical observation, the organisms treated with AL072 exhibited characteristic morphological changes in the cellular outer coat. Also irregular morphological changes, such as the formation of filamentous materials in the cytoplasm, an increase in the size and number of cytoplasmic vacuoles, the extruding of cytoplasmic contents, the formation of spheroplast and ghost cells, and blebbings in the cell wall were observed. Furthermore, immunoelectron microscopical observation of the group treated with the MIC showed that the immune complex attached mainly to the cell wall. The results of these experiments indicate that AL072, like the inhibitors of cell wall synthesis, act selectively on the cell wall of L. pneumophila.

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Morphological Variation of Lingulodinium polyedrum (Dinophyceae) in Culture Specimens and Reinterpretation of the Thecal Formula

  • Kim, Keun-Yong;Yoshida, Makoto;Kim, Chang-Hoon
    • ALGAE
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    • v.20 no.4
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    • pp.299-304
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    • 2005
  • Morphological observation of a unialgal culture of Lingulodinium polyedrum was conducted under light- and scanning electron microscopes. Lingulodinium polyedrum was redefined as having the thecal formula of APC, Q, 5', 6", 6c, 6s, 5'", 2"" using the reinterpreted tabulation system. In our tabulation system, the Q, which is equivalent to the 3' in the strict Kofoidean system, was treated as an auxiliary platelet because of its considerable morphological variations in culture specimens. Subsequently, apical plates were redefined as plates directly connected to the APC and/or the Q. The 1"' and the 1p in the conventional Kofoidean system were treated as a component of the sulcus (the Ssa) and as an antapical plate (the 1""), respectively. Our reinterpreted tabulation system clearly differentiated extant gonyaulacalean taxa.

Morphological research on radio loud AGN 4C39.25 using KaVA observation

  • Yoo, Hyemin;Sohn, Bong Won;Yi, Sukyong K.
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.2
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    • pp.36.3-37
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    • 2015
  • 4C39.25 (0923+392) is a distant radio loud AGN placed at redshift 0.695. The motivation of our work is peculiar properties 4C39.25. Firstly, it has a conspicuous distinction of jet direction between kilo-parsec scale observation made by VLA (Kollgaard et al. 1990) and the parsec scale observation by VLBA (Kellermann et al. 1998). This might indicate episodic-jet activity which recently turned on. This object currently shows two stationary compact parsec-scale components which are bright jet component on east and less luminous core on west. Also, it is known that there have been superluminal jet components which are flowing from the core toward east, and then merging with the bright jet component (Marscher et al. 1991, Alberdi et al. 2000, Lister et al. 2013). Although 4C39.25 seems to be a blazar-like source having broad emission lines (SDSS) and superluminal motion, its property that jet component is brighter than the core is different from ordinary blazars. Furthermore, it has young radio galaxy-like properties such as non-variation in total flux (Alberdi et al. 1997, 2000, MOJAVE database) and high frequency peak at spectral energy distribution (Orienti et al 2007). Such complex properties led us to make recent observations to reveal precise properties and new changes of the source. We used Korean VLBI Network (KVN) and VLBI Exploration of Radio Astronomy (VERA) Array (KaVA) which provide high-frequency (23GHz and 43GHz) and high spatial resolution (1.2mas and 0.6mas). Therefore, this system is suitable for morphological and physical research on parsec scale structure. We present results for several epochs observed during 2013 to 2014, mainly focusing on morphological changes of 4C39.25 using KaVA images.

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A Study on the Image Evaluation of Buildings in the University Campus (대학캠퍼스 건축물에 대한 이미지평가에 관한 연구)

  • Kim, Cheon-Il;Kim, Ick-Hwan
    • Journal of the Korean Institute of Educational Facilities
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    • v.19 no.3
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    • pp.13-20
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    • 2012
  • In this study, we performed the psychological analysis on campus facilities for university students with the observation that the evaluation on the physical environment of the campus is related to the image evaluation of the campus users. Specifically, we investigated the cognitive structure and the image evaluation structure of users, i.e., students, on the architectural and morphological elements of campus buildings. The investigation results are summarized as follows. 1) The cognitive structure of students on the architectural and morphological elements was different from building to building. In the case of architectural elements, the campus buildings were rated high, if they were designed with emphasis on symbolic elements, while the campus buildings with simple design were rated low. However, the morphological elements exhibited the opposite results. 2) We extracted six factors by performing the factor analysis for image evaluation. From the analysis results, we found that students showed a different structure of image evaluation for each building. In addition, the image evaluation structures were found to be related with the cognitive structures on architectural and morphological elements. 3) We also performed the correlation analysis between image evaluation structure and cognitive structure on architectural and morphological aspects. The analysis results indicated that each building showed different correlation patterns. In addition, the overall image became better, as they appraised "clarity" and "uniformity" more highly.