• Title, Summary, Keyword: mouse

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Enu is a Powerful Mutagen for Development Mutant Mice -Sixty-Six Mutants From Enu Mutagenesis Program in Kit/Krict-

  • Seokjoo Yoon;Cho, Kyu-Hyuk;Cho, Jae-Woo;Lee, Phil-Soo;Kim, Yang-Eon;Cha, Dal-Sun;Park, Han-Jin;Kang, Min-Sung;Nam, Yoon-Yi
    • Proceedings of the Korean Society of Toxicology Conference
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    • pp.184-184
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    • 2003
  • ENU(ethylnitrosourea) mutagenesis has been carrying out since 1999 in Korea Institute of Toxicology (KIT), Korea Research Institute Chemical of Technology (KRlCT). We have chosen BALB/c and C57BL/6 and screened for dominant and recessive mutants. Four hundred and twenty one males(GO) have been injected with ENU, 150, 200, 250 and 300 mg/kg body weight, twice, one week apart.(omitted)

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The effect of muscle activity wristbands with mouse using (컴퓨터 마우스 보조기구 사용이 근활성도에 미치는 영향)

  • Lee, Tae-Sik;Song, Min-Young;Chea, Il-Jun
    • Journal of Korean Physical Therapy Science
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    • v.19 no.1
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    • pp.47-54
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    • 2012
  • Purpose : This study is to know the effect on muscle activity when people use mouse aid or not in computer working environment. Method : 11 people of both sex in their twentieth were tested. As a independent variable, the condition without mouse aid and the condition with movable mouse aid were selected, and as a dependent variable, MVC(%) on four muscles and inconvenience degree(RPE scale) on three body part(wrist, forearm, shoulder) were measured. Result : Analyzing the research, MVC(%) of extensor muscle of finger had notably higher muscle activity than other muscles, and there wasn't notable difference in muscle activity before and after using mouse aid. In case of using mouse aid, we tested by giving RPE questionnaire which segmentalized mental fatigue degree into hand, forearm, and shoulder, and resultingly, there wasn't notable differences perspectively. However, people felt less tiredness subjectively after they use the mouse aid. Conclusion : The usage of mouse aid seems to give comfort to computer users.

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Effect of Testosterone on Free Radical Generating Enzyme and Lipid Peroxidation (지질과산화 반응과 Free Radical 생성계 효소활성에 미치는 Testosterone의 영향)

  • Huh, Keun;Shin, Uk-Seob;Park, Jong-Min
    • YAKHAK HOEJI
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    • v.38 no.2
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    • pp.166-173
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    • 1994
  • Sex hormones not only regulate external sexual characteristics but several internal biochemical processes. It is well accepted that life-span of female is longer than that of male. Life-span is closely related with aging process in which free radicals are known to be involved. We investigated the effect of testosterone on free radical generating systems and lipid peroxidation based on the sexual difference. Lipid peroxide levels of male and female mouse were increased proportionately with age, especially in male mouse. Increase in enzyme activity of aldehyde oxidase with age was observed in male mouse, while no siginificant change in enzyme activity was found in female mouse. Enzyme activity of xanthine oxidase also showed similar results. It, however, was not significant statistically. Lipid peroxide level and xanthine oxidase type conversion ratio of male and female mouse liver homogenate incubated at $37^{\circ}C$, increased remarkably in proportion to incubation time, especially in male mouse. Lipid peroxide level and aldehyde oxidase activity were measured in normal male mouse, castrated mouse and testosterone treated-castrated mouse. Castrated mouse group showed decrease in lipid peroxide level and aldehyde oxidase activity compared with normal group. Treatment of castrated mouse with testosterone, however turned the level of lipid peroxide and aldehyde oxidase activity back to normal. From the above results, it might be concluded that testosteron could increase the activities of free radical generating enzymes which would result in the formation of lipid peroxide, consequently leading to aging.

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Inhibition of DMBA-Induced Mouse Epidermal Carcinogenesis by Astaxanthin-Containing Egg Yolks (DMBA로 유발한 Mouse 피부암에 대한 Astaxanthin이 함유된 난황의 항암효과)

  • Lee, Sang-Ho;Park, Cheol-U;Lee, Yeong-Chun;Choe, Ui-Seong;Kim, Mu-Nam;Ha, Yeong-Rae
    • Environmental Mutagens and Carcinogens
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    • v.18 no.1
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    • pp.22-25
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    • 1998
  • Anticarcinogenic activity of astaxanthin-containing egg yolks (designate AEY) was investigated for 7,12-dimethylbenz[a]anthracene (DMBA)-induced two stage mouse epidermal carcinogenesis. Female ICR mouse (6-7 weeks of age) were house in a humidity-and-temperature-controlled facility and subjected to feed and water ad libitum. AEY (10 mg/0.2 ml acetone) was painted on the back of mice 7 days, 3 days and 5 min before DMBA treatment (50 nmole/0.2 ml acetone). One week later after DMBA treatment, 6 ${\mu}g$ tetradecanoyl 12-phorbol 13-O-acetate (TPA) dissolved in 0.2 ml acetone was applied on the mouse twice weekly over a period of 22 weeks. No sample was given to control mice. Control egg yolk (CEY) and astaxanthin-containing oil (designate AO) from Phaffia rhodozyma were used as positive controls. Mouse treated with AEY exhibited 10 tumors per mouse whereas control mouse exhibited 15 tumors per mouse, the fact that 33% reduction of tumor per mouse by AEY treatment. Tumor incidence was also reduced to 15% by AEY treatment when compared to that of control group. Such effects were also seen in CEY and AO treatment groups, but leaser extent. AO gave reduction of food intake and body weights relative to those of AEY and CEY, indicating toxicity of AO. These results suggest that AEY exhibits anticarcinogenic activity for DMBA-induced mouse epidermal carcinogenesis.

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A Study on the Splitting Methods in Mouse and Bovine Embryos (생쥐 및 소 수정란의 분할방법에 관한 연구)

  • 조남기
    • Korean Journal of Animal Reproduction
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    • v.11 no.2
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    • pp.127-131
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    • 1987
  • This study was carried out to obtain the basic information on splitting and culture of mouse and bovine embryos. Two-, four-, eight-, cell and morula mouse embryos were digested with pronase, splitted in vitro by micro-glass needel with hand, and bovine embryos were splitted by micromanipulator. The splitted embryos were cultured under 5% of CO2 gas in air at 37$^{\circ}C$ for 48-72 hours. The results obtained in this study were summarized as follows: 1. The mouse and cattle were superovulated by 5IU of PMS and HCG, and 2500IU of PMS and 25mg of PGF2$\alpha$, respectively. The average number of embryos after superovulation were 32.5$\pm$8.2 and 7.5$\pm$3:1, respectively. 2. Out of total 122 embryos splitted, the successful splitting rate was 75.0%, 66.7%, 68.4% and 71.4% in 2-, 4-, 8- and morula embryos in mouse, respectively. There was no different splitting rate between mouse(71.4%) and bovine embryos(66.7%) in morula. 3. The successful culture rate of splitted embryos was 68.0% and 67.9% in mouse and bovine embryos, respectively.

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Effects of Oriental medicine on Osteoporosis in Ovariectomized Rats

  • Kim, Young-Ock;An , Deuk-Kyun;Lee, Hyun-Sun;Lee, Young-Ah;Kim, Yang-On;Song, Chang-Woo;Kim, Yang-Eon;Song, Chang-Woo
    • Proceedings of the PSK Conference
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    • pp.233.2-233
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    • 2003
  • This experiment study was carried out to prove the efficacy of Cheongpajeon(CPJ) extract in ovariectomized rats. 40 rats were divided into 4 groups, administrated saline after sham operation group(sham-op), administered saline after ovariectomy group(control), administered CPJ 1g/kg after ovariectomy group and administered Livial 0.042mg/kg after ovariectomy group(positive control). We examined the water extract of CPJ that is capable of affecting osteoblast proliferation using MG-63 and HOS-TE85. (omitted)

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Optimal Display-Control Gain of the Foot-Controlled Isotonic Mouse on a Target Acquisition Task (목표점 선택작업에서 등력성 발 마우스의 최적 반응 - 조종 이득)

  • Lee, Kyung-Tae;Jang, Phil-Sik;Lee, Dong-Hyun
    • IE interfaces
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    • v.17 no.1
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    • pp.113-120
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    • 2004
  • The increased use of computers has introduced a variety kind of human-computer interfaces. Mouse is one of the useful interface tools to place the cursor on the desired position on the monitor. This paper suggested a foot controlled isotonic mouse which was similar to the ordinary hand-controlled mouse except that positioning was controlled by the right foot and the clicking was performed by the left foot. Experimental results showed that both the index of difficulty(IOD) and the display-control gain(DC gain) varied the total movement time in a target acquisition task on the monitor. The present authors also drew the optimal display-control gain of the foot-controlled isotonic mouse over the index of difficulty of 1.0 to 3.0. The optimal display-control gain, i. e., 0.256, could be used when designing a foot-controlled isotonic mouse.

Increased Antitumor Immunity of Mouse GM-CSF in Mouse Colon Tumor (CT-26) Model

  • Kim, Mi Kyung;Lee, Yu Kyoung;Lee, Yeon Sook;Hwang, Tae Ho
    • Biomedical Science Letters
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    • v.19 no.4
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    • pp.303-309
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    • 2013
  • Oncolytic vaccinia virus is an engineered vaccinia virus that selectively destroys cancer cells and induces tumor immune response. Oncolytic vaccinia expressing mouse GM-CSF showed cytotoxic activity against various kinds of cancer cells when oncolytic vaccinia virus expressing human GM-CSF and mouse GM-CSF is intravenously administered in the mouse CT26 colon tumor model. Cancer cells treated with isolated immunoglobulin G from the serum with complement showed these cytotoxic activity and complement observed dose-dependent cytotoxic effect. These results suggest that oncolytic vaccinia virus expressing mouse GM-CSF can increase oncolytic vaccinia virus by inducing anticancer antibody in a mouse tumor model. Further studies are needed on antitumor immunity of GM-CSF.

Studies on In vitro Preservation of the Mouse and Bovine Embryos (생쥐 및 소초기배의 체외보존에 관한 연구)

  • Kweon Oh-Kyeong
    • Journal of Veterinary Clinics
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    • v.8 no.1
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    • pp.103-108
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    • 1991
  • It was carried out to investigate the effect of the kind of medium, the concentration of serum added and the temperature of storage on the survival of mouse and bovine embryos preserved In vitro. The survival of frozen-thawed mouse embryos after cooling at 4$^{\circ}C$ was also investigated. It was possible to preserve the embryos of mouse until 4 days and of cattle in a day without significant decrease of the survival rates. The survival rates of frozen-thawed mouse embryos after cooling at 4$^{\circ}C$ over 3 days were below 20%.

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Gene expression profile of hypothalamus isolated in different nutrient feeding mouse (고지방식이를 섭취한 mouse의 hypothalamus에서의 유전자군의 발현양상 변화)

  • Cha, Min-Ho;Kim, Kyung-Seon;Kang, Bong-Joo;Sim, Woong-Seop;Yoon, Yoo-Sik
    • Korean Journal of Oriental Medicine
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    • v.9 no.1
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    • pp.145-156
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    • 2003
  • Obesity is caused by imbalance of energy intake and expense. If energy intake is more than its expenditure, body does fat accumulation and affects body weight. It can be fetal disease although obesity is not disease in itself. Central regulatary system is affected by many neurotransmitters regulating .food intake in brain. Hypothalamus was known as one of food intake regulation in CNS. In order to investigate gene expression difference in hypothalamus by different nutrient, we used C57/BL6 control mouse and db-/db- mouse. They divided each of two group with mouse, and fed control diet and high-fat diet for 4 weeks. Each of control and high-fat diet contained 11.7% and 59.7% fat, respectively. Then we performed microarray assay with them. We compared among changed genes in hypothalamus region. In the results, we observed that increased genes were more than decreased genes. Although hypothalamus size of db-/db- mouse is smaller than that of C57/BL6, more genes were affected in db-/db- mouse. In this study, many genes are affected by nutrient in hypothalamus region.

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