• 한국가축번식학회지
• /
• 제11권2호
• /
• pp.127-131
• /
• 1987

This study was carried out to obtain the basic information on splitting and culture of mouse and bovine embryos. Two-, four-, eight-, cell and morula mouse embryos were digested with pronase, splitted in vitro by micro-glass needel with hand, and bovine embryos were splitted by micromanipulator. The splitted embryos were cultured under 5% of CO2 gas in air at 37$^{\circ}C$ for 48-72 hours. The results obtained in this study were summarized as follows: 1. The mouse and cattle were superovulated by 5IU of PMS and HCG, and 2500IU of PMS and 25mg of PGF2$\alpha$, respectively. The average number of embryos after superovulation were 32.5$\pm$8.2 and 7.5$\pm$3:1, respectively. 2. Out of total 122 embryos splitted, the successful splitting rate was 75.0%, 66.7%, 68.4% and 71.4% in 2-, 4-, 8- and morula embryos in mouse, respectively. There was no different splitting rate between mouse(71.4%) and bovine embryos(66.7%) in morula. 3. The successful culture rate of splitted embryos was 68.0% and 67.9% in mouse and bovine embryos, respectively.

• 대한의생명과학회지
• /
• 제19권4호
• /
• pp.303-309
• /
• 2013

Oncolytic vaccinia virus is an engineered vaccinia virus that selectively destroys cancer cells and induces tumor immune response. Oncolytic vaccinia expressing mouse GM-CSF showed cytotoxic activity against various kinds of cancer cells when oncolytic vaccinia virus expressing human GM-CSF and mouse GM-CSF is intravenously administered in the mouse CT26 colon tumor model. Cancer cells treated with isolated immunoglobulin G from the serum with complement showed these cytotoxic activity and complement observed dose-dependent cytotoxic effect. These results suggest that oncolytic vaccinia virus expressing mouse GM-CSF can increase oncolytic vaccinia virus by inducing anticancer antibody in a mouse tumor model. Further studies are needed on antitumor immunity of GM-CSF.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
• /
• pp.233.2-233.2
• /
• 2003

This experiment study was carried out to prove the efficacy of Cheongpajeon(CPJ) extract in ovariectomized rats. 40 rats were divided into 4 groups, administrated saline after sham operation group(sham-op), administered saline after ovariectomy group(control), administered CPJ 1g/kg after ovariectomy group and administered Livial 0.042mg/kg after ovariectomy group(positive control). We examined the water extract of CPJ that is capable of affecting osteoblast proliferation using MG-63 and HOS-TE85. (omitted)

• 한국임상수의학회지
• /
• 제8권1호
• /
• pp.103-108
• /
• 1991

It was carried out to investigate the effect of the kind of medium, the concentration of serum added and the temperature of storage on the survival of mouse and bovine embryos preserved In vitro. The survival of frozen-thawed mouse embryos after cooling at 4$^{\circ}C$ was also investigated. It was possible to preserve the embryos of mouse until 4 days and of cattle in a day without significant decrease of the survival rates. The survival rates of frozen-thawed mouse embryos after cooling at 4$^{\circ}C$ over 3 days were below 20%.

• BMB Reports
• /
• 제55권7호
• /
• pp.360-360
• /
• 2022

• 한국산림과학회지
• /
• 제94권2호통권159호
• /
• pp.108-111
• /
• 2005

This study was conducted to identify the road effect on small rodent populations within fragmented forest areas around the road from June to September 2002, in 9 study sites of Baekdugdaegan mountains, Korea. Two species of small rodents, Korean field mouse Apodemus peninsulae and striped field mouse Apodemus agrarius, were captured in this study. Korean field mouse preferred forest area, and striped field mouse generally has been found edge area around road. Mean body weight of Korean field mouse was significantly different, but that of striped field mouse was not between both distance from road. Korean field mouse is forest-dwelling species and their distribution is limited in forest area. In contrast, striped field mouse has wide distributional range around road. The effects of road is different in each small rodent species and their habitat preferences.

• Parasites, Hosts and Diseases
• /
• 제22권2호
• /
• pp.253-258
• /
• 1984

Naegleria fowleri를 마우스 비강을 통하여 감염시킨 뒤 P.A.M. 발생율 및 발병후 생존기간을 마우스 주별 체중별, 성별, 접종아메 바수에 따라 비교하여 다음과 같은 성적을 얻었다. 1. N. fewleri를 감염시킨후 P.A.M. 발생은 ICR마우스보다 BALB/C마우스에서 많았으며 감염후 평균생존기간은 차이가 없었다. 2. 마우스 성별에 따른 N. fowleri감염후 P.A.M.발생율 및 발병후 평균생존기간은 차이가 없었다. 3. 체중이 다른 여러 실험군에서 P.A.M. 발생율 및 사망율은 차이가 없었으나 발병후 마우스의 평균생존 기간은 체중이 가벼운 실험군에서 짧았다. 4. 감염시킨 아메 바수에 따른 P.A.M. 발생율은 영양형 Ix104이상 감염 군에서는 차이가 없었으나 0.5x104인 경우 P.A.M 발생이 현저히 감소하였다.

• Interdisciplinary Bio Central
• /
• 제2권4호
• /
• pp.16.1-16.4
• /
• 2010

Cryopreservation of mouse embryos and spermatozoa has become the foremost technique for preserving large numbers of different strains of mice with induced mutations. In 1998, our mouse bank was established in the Center for Animal Resources and Development (CARD), Institute of Resource Development and Analysis, Kumamoto University, Japan, based on the Preservation, supply and development of genetically engineered animals report published by the Ministry of Education, Culture, Sports, Science and Technology. We cryopreserve mouse embryos and sperm, supply these resources, organize training courses to educate people and form part of a domestic and international network of both mutagenesis and resource centers. We currently have over 1,500 mouse strains, 842,000 frozen embryos and 26,000 straws containing frozen sperm. Moreover, we disclose information about 1,300 deposited strains. Furthermore, over 400 strains of frozen embryos or mice produced from frozen embryos and sperm are being supplied to the requesters both domestically and internationally. Additionally we hold training courses on the cryopreservation of mouse germplasm 2~3 times a year, both domestically and internationally. In the course, we teach basic reproductive engineering techniques to trainees on a man-to-man basis. We have already held 28 training courses on the cryopreservation of mouse germplasm at our center and at other institutes.

• 한국한의학연구원논문집
• /
• 제9권1호
• /
• pp.145-156
• /
• 2003

Obesity is caused by imbalance of energy intake and expense. If energy intake is more than its expenditure, body does fat accumulation and affects body weight. It can be fetal disease although obesity is not disease in itself. Central regulatary system is affected by many neurotransmitters regulating .food intake in brain. Hypothalamus was known as one of food intake regulation in CNS. In order to investigate gene expression difference in hypothalamus by different nutrient, we used C57/BL6 control mouse and db-/db- mouse. They divided each of two group with mouse, and fed control diet and high-fat diet for 4 weeks. Each of control and high-fat diet contained 11.7% and 59.7% fat, respectively. Then we performed microarray assay with them. We compared among changed genes in hypothalamus region. In the results, we observed that increased genes were more than decreased genes. Although hypothalamus size of db-/db- mouse is smaller than that of C57/BL6, more genes were affected in db-/db- mouse. In this study, many genes are affected by nutrient in hypothalamus region.

• 대한한의학회지
• /
• 제19권1호
• /
• pp.327-338
• /
• 1998

To investigate effect of water extract of PaIMuITang(PMT) on human cancer cell-lines and immunocytes, this research estimated proliferation of A431 cell line, KHOS-NP cell line, mouse thymocytes and mouse splenocytes, Nitric Oxide(NO) from macrophage, apoptosis and subpopulation of the mouse thymocytes. The results were obtained as follows; 1. PMT inhibited the. proliferation of A431 cell line, but it is not significant. 2. PMT inhibited the proliferation of KHOS-NP cell line, but it is not significant. 3. PMT stimulated the proliferation of mouse thymocytes, being compared Con A non-treated group. 4. PMT stimulated the proliferation of mouse splenocytes, being compared LPS treated group. 5. PMT l00g/mQ inhibited the production of NO from macrophages in vitro, being compared NPS IFN treated group. 6. PMT inhibited the production of NO from macrophages in vivo, being compared LPS|IFN treated group. 7. PMT accelerated the induction of apoptosis of the mouse thymocytes. 8. In subpopulation PMT decreased $T_H$ of the mouse thymocytes, but increased T /dT s of the mouse thymocytes.