• Title, Summary, Keyword: mouse

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Experimental Meningoencephalitis by Nuegleria fowleri in Mice (마우스에서 Naegleria fowleri에 의한 뇌수막염 발생에 관한 실험적 연구)

  • 안명희;임경일
    • The Korean Journal of Parasitology
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    • v.22 no.2
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    • pp.253-258
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    • 1984
  • Experimentally, primary amoebic meningoencephalitis (PAM) is induced by Naegleria fowleri in mouse and development of PAM may be inauenced by the strain, weight and sex of mouse, and inoculum size of N. fowleri trophozoite. In this paper, the effect of these factors on PAM development of mouse was studied. N. fowleri trophozoites, strain 0359, were introduced into mouse intranasally under secobarbital anesthesia (0.05mg/g). 1. PAM was developed more frequently in BALB/C mouse than ICR mouse. 2. The survival time of mouse with PAM was influenced by the weight, that is, it was shorter in 15 g mouse than in the heavier groups. 3. No difEerence was observed on PAM development according to sect. 4. In case of inoculated amoeba, PAM incidence of $0.5{\times}10^4$ was markedly decreased.

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A Study of Muscle Activation in Upper Extremity According Mouse Shape (마우스 형태에 따른 상지의 근활성도의 변화)

  • Kim, Ju Heon;Yu, Yeon Tae;Kim, Jin Hun;Oh, Tae Young
    • Journal of Korean Physical Therapy Science
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    • v.19 no.4
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    • pp.53-59
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    • 2012
  • Background : The purpose of the study was: to investigate muscle activation of upper arm according mouse shape. Methods : Twenty person(mean age : 23. 7) who have healthy condition was participated this study, we collected data of muscle activation using by EMG from upper trapezius(Tr), deltoid middle fiber(De), extensor digitorum(Ed), first dorsal interosseous(Di) during participants was performed click and drag according various mouse. Mouse shape was divided 4 level as follow shape 1 was very small, 2 was small, 3 was moderate, 4 was large. Data was analyzed ANOVA, independent t-test using by SPSS ver18.0. Results : There was significantly difference of muscle activation among each muscle according mouse shape in drag and click. In shape 1, 4, there was significantly difference of muscle activation of Tr, De, Ed between drag and click except Di. In shape 2, 4, there was significantly difference of muscle activation of all muscle between drag and click. Conclusion : We knew that extensor digitroum showed more higher muscle activation than other muscle in drag, first dorsal interoseous showed more higher muscle activation that other muscle in click. We suggest that mouse shape was very important factor in order to prevent skeletal muscular disorder for computer user, and mouse shape can reduce muscle fatigue during computer work.

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Overexpression of Mouse Nck Transforms Mouse Febroblast NIH3T3

  • Kim, Young H.;Han, Sun-Mi;Kim, Moon G.;Park, Dong-Eun;Park, Sang D.;Seong, Rho H.
    • Animal cells and systems
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    • v.1 no.3
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    • pp.521-526
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    • 1997
  • We isolated a mouse nck cDNA from the thymus cDNA expression library. The cDNA encodes a 377 amino acid protein and displays 97% amino acid sequence identity to human oncogenic protein nck, which is composed almost exclusivelv of three src homology 3 (SH3) domains and one SH2 domain. The sequence analysis also showed that the isolated cDNA is the mouse counterpart of the human nck and different from the mouse grb4, which has been reported to be highly similar to the human nck and, therefore considered as a mouse nck, Northern blot analysis showed that the transcript of the gene was 1.8 kb and was highly expressed in the testis, thymus, and brain but moderately in the liver and lymph node. Western blot analysis showed that the size of the protein was about 47 kDa. Overexpression of the mouse Nck transformed a mouse fibroblast cell line, NIH3T3. The results clearly indicate that normal nck gene has transforming ability and provide an argument against a suggested possibility that the transforming ability of the human nck gene is due to a mutation(s) in the gene.

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Mouse mannose-binding lectin-A and ficolin-A inhibit lipopolysaccharide-mediated pro-inflammatory responses on mast cells

  • Ma, Ying Jie;Kang, Hee Jung;Kim, Ji Yeon;Garred, Peter;Lee, Myung-Shik;Lee, Bok Luel
    • BMB Reports
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    • v.46 no.7
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    • pp.376-381
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    • 2013
  • It is unknown how soluble pattern-recognition receptors in blood, such as mannose-binding lectin (MBL) and ficolins, modulate mast cell-mediated inflammatory responses. We investigate how mouse MBL-A or ficolin-A regulate mouse bone marrow-derived mast cells (mBMMCs)-derived inflammatory response against bacterial lipopolysaccharide (LPS) stimulation. LPS-mediated pro-inflammatory cytokine productions on mBMMCs obtained from Toll-like receptor4 (TLR4)-deficient mice, TLR2-defficient mice, and their wildtype, were specifically attenuated by the addition of either mouse MBL-A or ficolin-A in a dose-dependent manner. However, the inhibitory effects by mouse MBL-A or ficolin-A were restored by the addition of mannose or N-acetylglucosamine, respectively. These results suggest that mouse MBL-A and ficolin-A bind to LPS via its carbohydrate-recognition domain and fibrinogen-like domain, respectively, whereby cytokine production by LPS-mediated TLR4 in mBMMCs appears to be down-regulated, indicating that mouse MBL and ficolin may have an inhibitory function toward mouse TLR4-mediated excessive inflammation on the mast cells.

The Experimetal Effects of PalMulTang on Anti-Cancer and Immunologic Function (팔물탕(八物湯)이 항암(抗癌) 및 면역기능(免疫機能)에 미치는 실험적(實驗的) 효과(效果))

  • Park, Hae-Jun;Ko, Woo-Shin
    • The Journal of Korean Medicine
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    • v.19 no.1
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    • pp.327-338
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    • 1998
  • To investigate effect of water extract of PaIMuITang(PMT) on human cancer cell-lines and immunocytes, this research estimated proliferation of A431 cell line, KHOS-NP cell line, mouse thymocytes and mouse splenocytes, Nitric Oxide(NO) from macrophage, apoptosis and subpopulation of the mouse thymocytes. The results were obtained as follows; 1. PMT inhibited the. proliferation of A431 cell line, but it is not significant. 2. PMT inhibited the proliferation of KHOS-NP cell line, but it is not significant. 3. PMT stimulated the proliferation of mouse thymocytes, being compared Con A non-treated group. 4. PMT stimulated the proliferation of mouse splenocytes, being compared LPS treated group. 5. PMT l00g/mQ inhibited the production of NO from macrophages in vitro, being compared NPS IFN treated group. 6. PMT inhibited the production of NO from macrophages in vivo, being compared LPS|IFN treated group. 7. PMT accelerated the induction of apoptosis of the mouse thymocytes. 8. In subpopulation PMT decreased $T_H$ of the mouse thymocytes, but increased T /dT s of the mouse thymocytes.

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Mouse Bank at CARD Kumamoto University, Japan

  • Nakagata, Naomi
    • Interdisciplinary Bio Central
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    • v.2 no.4
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    • pp.16.1-16.4
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    • 2010
  • Cryopreservation of mouse embryos and spermatozoa has become the foremost technique for preserving large numbers of different strains of mice with induced mutations. In 1998, our mouse bank was established in the Center for Animal Resources and Development (CARD), Institute of Resource Development and Analysis, Kumamoto University, Japan, based on the Preservation, supply and development of genetically engineered animals report published by the Ministry of Education, Culture, Sports, Science and Technology. We cryopreserve mouse embryos and sperm, supply these resources, organize training courses to educate people and form part of a domestic and international network of both mutagenesis and resource centers. We currently have over 1,500 mouse strains, 842,000 frozen embryos and 26,000 straws containing frozen sperm. Moreover, we disclose information about 1,300 deposited strains. Furthermore, over 400 strains of frozen embryos or mice produced from frozen embryos and sperm are being supplied to the requesters both domestically and internationally. Additionally we hold training courses on the cryopreservation of mouse germplasm 2~3 times a year, both domestically and internationally. In the course, we teach basic reproductive engineering techniques to trainees on a man-to-man basis. We have already held 28 training courses on the cryopreservation of mouse germplasm at our center and at other institutes.

Improving Eye-gaze Mouse System Using Mouth Open Detection and Pop Up Menu (입 벌림 인식과 팝업 메뉴를 이용한 시선추적 마우스 시스템 성능 개선)

  • Byeon, Ju Yeong;Jung, Keechul
    • Journal of Korea Multimedia Society
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    • v.23 no.12
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    • pp.1454-1463
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    • 2020
  • An important factor in eye-tracking PC interface for general paralyzed patients is the implementation of the mouse interface, for manipulating the GUI. With a successfully implemented mouse interface, users can generate mouse events exactly at the point of their choosing. However, it is difficult to define this interaction in the eye-tracking interface. This problem has been defined as the Midas touch problem and has been a major focus of eye-tracking research. There have been many attempts to solve this problem using blink, voice input, etc. However, it was not suitable for general paralyzed patients because some of them cannot wink or speak. In this paper, we propose a mouth-pop-up, eye-tracking mouse interface that solves the Midas touch problem as well as becoming a suitable interface for general paralyzed patients using a common RGB camera. The interface presented in this paper implements a mouse interface that detects the opening and closing of the mouth to activate a pop-up menu that the user can select the mouse event. After implementation, a performance experiment was conducted. As a result, we found that the number of malfunctions and the time to perform tasks were reduced compared to the existing method.

Characterization of Brain Tumor Cell using Vasopressin-SV40 T Ag Transgenic Mouse

  • Kim, Sung-Hyun;Lee, Eun-Ju;Kim, Myoung-Ok;Park, Jun-Hong;Kyoungin-Cho;Jung, Boo-Kyung;Kim, Hee-Chul;Hwang, Sol-Ha;Lee, Hoon-Taek
    • Proceedings of the KSAR Conference
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    • pp.44-44
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    • 2003
  • In previous reports, pVPSV.IGR2.1 transgenic mouse were described that brain tumor and lymphoma by reason of Vasopressin-SV40 T antigen. In this study, we produced pVPSV.IGR3.6 transgenic mouse that used pVPSV.IGR3.6 vector. Expression of transgene was vary different in transgenic mouse. We obtained 6 transgenic mouse line, moreover they had died at the age of 2~6 weeks without transmitting the transgene to their offspring, and had tumorigenesis on same location with pVPSV.IGR2.1 transgenic mouse. Only a founder mouse was investigated for expression of fusion gene. Here we extended this transgenic approach to the study of tumor progression. From the mouse, we confirmed brain tumor cell, after then cultured for investigate characterization. In this report, we demonstrate that reduction of survival rate in transgenic mouse fused vasopressin gene length, acquisition of brain tumor cell, composition with astrocyte cells and neuronal cells. Finally, cells had no change with increase of passage.

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Effect of Epidermal Growth Factor(EGF) on Early Embryonic Development in Mouse (Epidermal Growth Factor(EGF)가 생쥐 초기배아의 발생에 미치는 영향)

  • Byun, Hye-Kyung;Lee, Ho-Joon;Kim, Sung-Rye;Kim, Hae-Kwon;Kim, Moon-Kyoo
    • Clinical and Experimental Reproductive Medicine
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    • v.22 no.2
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    • pp.163-170
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    • 1995
  • Growth factors (GFs) produced by the embryo or by the maternal reproductive tract have been reported to regulate the embryonic development and differentiation. Among GFs, EGF as a mitogen plays a role in mitosis and functional differentiation of trophectoderm cells in mouse. The present study was carried out to investigate the effect of EGF on development of mouse embryos and to localize EGF in the mouse oocytes and embryos, which has been reported to be detected in the reproductive tract in mammals. To investigate the effect of EGF on the development of the embryo, mouse 2-cell embryos were cultured to blastocysts stage in Ham's F10 medium, treated with EGF(10-50 ng/ml) for 72 hrs. Immunocytochemistry was performed from oocyte to blastocyst stage with anti-EGF and anti-Mouse IgG, in order to determine the stage which EGF would be expressed in mouse. Exogenous EGF (more than 10 ng/ml) in the culture medium improved the developmental and hatching rates in the mouse embryos. As a result of immunocytochemistry, the embryonic EGF was expressed after the late 4-cell stage. EGF is thought to enhance preimplantation embryonic development and hatching. Exogenous EGF in the culture medium is thought to activate EGF receptor in the late 4-cell embryos and to enhance blastulation and hatching in mouse embryos. It is concluded that EGF enhances the developmental and hatching rates in the mouse embryos.

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Effect of Mature Human Follicular Fluid on the Development of Mouse Embryos in vitro (성숙난포액을 이용한 생쥐배아의 발달에 관한 연구)

  • Park, S.Y.;Lee, J.J.;Kim, S.H.;Ku, P.S.
    • Clinical and Experimental Reproductive Medicine
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    • v.19 no.2
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    • pp.125-131
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    • 1992
  • The possible effect of human follicular fluid(hFF) on the growth and development of fertilized oocytes and embryos is important because the fallopian tubes are exposed to FF after follicular rupture and the processes of fertilization and embryo cleavage occur inside the fallopian tubes. Previously, it was suggested that human FF might adversely affect on the development of early mouse embryos. In order to investigate the effect of hFF on the development of embryos, early mouse embryos were cultured in media containing various protein sources as bovine serum albumin(BSA), fetal cord serum(FCS) and FF. And we evaluated the development of early mouse embryos in terms of the morphology, cleavage rate, and cell count of blastcysts. There were no significant differences in the morula and blstocyst formation rates of 2-cell mouse embryos cultured in the media containg three different protein sources and three different concentrations of FF. The blastocyst formation rate of 1-cell mouse embryo cultured in FF group was significantly higher than that cultured in BSA group(P<0.05). The morula and blastocyst formation rates of 2-cell mouse embryos of the group cultured in the media containing FF were comparable with those of other two groups, in addition, the cell count of blastocysts of FF group in the 2-cell embryo culture was higher than those of BSA group and HCS group(P<0.01), and this finding was also noted in 1-cell embryo culture. There was no difference in the morula and blastocyst formation rates of the 2-cell mouse embryos cultured in the media containing different concentrations of FF. These results suggest that mature human follicular fluid has no inhibitory activity on the development of early mouse embryos even in high concentration and may be a good protein source which is positively associated with the development of mouse embryos in vitro especially in 1 cell embryo culture.

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