• 제목/요약/키워드: mouse

검색결과 8,668건 처리시간 0.031초

Characterization of Bovine NANOG5'-flanking Region during Differentiation of Mouse Embryonic Stem Cells

  • Jang, Hye-Jeong;Park, Hwan Hee;Tran, Thi Thuy Linh;Lee, Hak-Kyo;Song, Ki-Duk;Lee, Woon Kyu
    • Asian-Australasian Journal of Animal Sciences
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    • 제28권12호
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    • pp.1721-1728
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    • 2015
  • Embryonic stem cells (ESCs) have been used as a powerful tool for research including gene manipulated animal models and the study of developmental gene regulation. Among the critical regulatory factors that maintain the pluripotency and self-renewal of undifferentiated ESCs, NANOG plays a very important role. Nevertheless, because pluripotency maintaining factors and specific markers for livestock ESCs have not yet been probed, few studies of the NANOG gene from domestic animals including bovine have been reported. Therefore, we chose mouse ESCs in order to understand and compare NANOG expression between bovine, human, and mouse during ESCs differentiation. We cloned a 600 bp (-420/+181) bovine NANOG 5'-flanking region, and tagged it with humanized recombinant green fluorescent protein (hrGFP) as a tracing reporter. Very high GFP expression for bovine NANOG promoter was observed in the mouse ESC line. GFP expression was monitored upon ESC differentiation and was gradually reduced along with differentiation toward neurons and adipocyte cells. Activity of bovine NANOG (-420/+181) promoter was compared with already known mouse and human NANOG promoters in mouse ESC and they were likely to show a similar pattern of regulation. In conclusion, bovine NANOG 5-flanking region functions in mouse ES cells and has characteristics similar to those of mouse and human. These results suggest that bovine gene function studied in mouse ES cells should be evaluated and extrapolated for application to characterization of bovine ES cells.

MOUSE 모형을 이용한 관거 내 고형물 퇴적량 산정 (Estimation of Solid Deposition in Sewer Systems using MOUSE Model)

  • 이재수
    • 한국수자원학회논문집
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    • 제40권5호
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    • pp.397-407
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    • 2007
  • 건기 때의 합류식 관거 내 고형물의 퇴적으로 인해 통수능이 감소하여 여름철 우기시 국지적인 침수가 발생하며 이로 인해 관거 내 퇴적을 더욱 초래할 수가 있다. 이와 같은 문제를 해결하고 관거 시스템을 효율적으로 관리하기 위해서는 관거 내 고형물 퇴적량을 산정하기 위한 식을 개발할 필요가 있다. 그러나 이러한 산정식을 개발하기 전에 컴퓨터 모형 등을 이용한 고형물 퇴적량을 산정하여야 한다. 따라서 본 연구에서는 국내 군자매수유역에 대해 MOUSE 모형을 적용하여 관거 내 고형물 퇴적량을 산정하였으며, 이를 미환경보호청(EPA)에서 개발한 산정식을 이용하여 산정한 퇴적량과 검토하였다. MOUSE 모형을 적용하여 산정한 값은 EPA에서 1977년에 개발한 초기의 산정식에 의한 결과보다는 작지만 1984년에 개발된 산정식에 의한 결과보다는 다소 크게 나타나고 있다. 퇴적고형물의 관측자료가 구비되어 있지 않아 모형에 의한 산정치를 비교하기는 곤란하지만 모형에 의해 산정결과가 신뢰성이 있다고 판단되며, 추후 군자배수유역에 대한 산정식을 유도하는데 이용할 수 있다고 본다.

Anti-Angiogenic Activity of Mouse N-/C-terminal deleted Endostatin

  • Cho, Hee-Yeong;Kim, Woo-Jean;Lee, Sae-Won;Kim, Young-Mi;Choi, Eu-Yul;Park, Yong-Suk;Kwon, Young-Guen;Kim, Kyu-Won
    • BMB Reports
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    • 제34권3호
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    • pp.206-211
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    • 2001
  • Endostatin, a proteolytic fragment of collagen XVIII, is a potent inhibitor of angiogenesis and the growth of several primary tumors. However, the opinions on the activity of endostatin derivatives deleted N- or C- terminal are still controversial. In this regard, we produced mouse endostatin and its derivatives in the prokaryotic system, and studied their anti-tumor activity. The [$^3H$]-thymidine incorporation assay demonstrated that N-terminal deleted mouse endostatin, and a C- and N-terminal deleted mutant, effectively inhibited the proliferation of human umbilical vein endothelial cells (HUVECs). The biological activity of endostatin was also shown by its in vivo anti-angiogenic ability on the chorioallantoic membrane (CAM) of a chick embryo. Treatment of $200\;{\mu}g$ of mouse endostatin, or N-terminal deleted mouse endostatin, inhibited capillary formation of CAM 45 to 71%, which is comparative to a 80% effect of positive control, $1\;{\mu}g$ of retinoic acid. An in vivo mouse tumor growth assay showed that N-terminal deleted mouse endostatin, and the N-/C-terminal deleted mutant, significantly repressed the growth of B16F10 melanoma cells in mice as did the full-length mouse endostatin. According to these results, N-and N-/C-terminal deleted mouse endostatins are the potent inhibitors of tumor growth and angiogenesis.

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부풀어지는 마우스: 기압센서를 이용한 입력과 햅틱 피드백을 갖는 부피가 변하는 마우스 (Inflatable Mouse: Volume-adjustable Mouse with Air-pressure-sensitive Input and Haptic Feedback)

  • 김석태;이보람;김현정;남택진;이우훈
    • 한국HCI학회:학술대회논문집
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    • 한국HCI학회 2008년도 학술대회 2부
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    • pp.323-328
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    • 2008
  • "부풀어지는 마우스"는 부피가 변하는 유저 인터페이스 기기이다. 이 마우스는 우리가 흔히 사용하는 마우스의 부피만큼 부풀어지는 것뿐만 아니라 사용하지 않을 때에는 납작하게 줄어들어 노트북 컴퓨터의 PC 카드 슬롯에 보관될 수 있다. 이 기기는 일반 마우스와 동일하게 사용 가능할 뿐만 아니라 마우스 풍선 안의 기압을 측정함으로써 새로운 인터랙션을 제공해 주고 있다. 또한 이 기기는 기존에 압력 센싱 인터랙션이 갖고 있는 한계점인 양방향 제어와 효율적인 피드백의 부재를 해결하고 있다. 나아가 제어 수단뿐만 아니라 표현의 수단으로도 활용 가능하다. 본 논문에서는 부풀어지는 마우스의 프로토타입 디자인에 대해서 설명하고 압력제어를 통해 줌인과 같은 응용 가능한 시나리오를 소개하고자 한다. 또한, 감성 및 커뮤니케이션 도구로의 활용 가능성도 기대 할 수 있다.

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An Arachidonic Acid Metabolizing Enzyme, 8S-Lipoxygenase, in Mouse Skin Carcinogenesis

  • Kim Eun-Jung
    • Nutritional Sciences
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    • 제9권3호
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    • pp.212-226
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    • 2006
  • The involvement of arachidonic acid (AA) metabolizing enzyme, lipoxygenase (LOX), in the development of particular tumors in humans has gradually been acknowledged and LOX has emerged as a novel target to prevent or treat human cancers. In the mouse skin carcinogenesis model, which provides an excellent model to study multistage nature of human cancer development, many studies have shown that some of the LOXs are constitutively upregulated in their expression. Moreover, application of LOX inhibitors effectively reduced tumor burdens, which implicates the involvement of LOX in mouse skin tumor development as well. 8S-LOX is a recently cloned LOX, which is specifically expressed in mouse skin after 12-O-tetradecanoyl-phorbol-13-acetate (TPA) treatment but not in normal skin. Unlike other members of the LOX 'family' expressed in mouse skin, this TPA-induced expression of 8S-LOX is prominent only in the skin of the TPA tumor promotion-sensitive strains of mice (SENCAR, CD-1, and NMRI) but not in the promotion-resistant C57BL/6J mice. This is a very unique phenomenon among strains of mice. Constitutive upregulation of 8S-LOX was also found in early stage papillomas and the expression was gradually reduced as the tumors became malignant. Based on these observations, it has been thought that 8S-LOX is involved in TPA-induced tumor promotion as well as in tumor conversion from papillomas to carcinomas. In accordance with this hypothesis, several studies have suggested possible roles of 8S-hydroxyeicosatetraenoic acid (HETE), an AA metabolite of 8S-LOX, in mouse skin tumor development. A clastogenic activity of 8S-HETE was demonstrated in primary keratinocytes and a close correlation between the levels of etheno-DNA adducts and 8S-HETE during skin carcinogenesis was also reported. On the other hand, it has been reported that 8S-LOX protein expression is restricted to a differentiated keratinocyte compartment Moreover, reported findings on the ability of 8S-HETE to cause keratinocyte differentiation appear to be contrary to the procarcinogenic features of the 8S-LOX expression, presenting a question as to the role of 8S-LOX during mouse skin carcinogenesis. In this review, molecular and biological features of 8S-LOX as well as current views on the functional role of 8S-LOX/8S-HETE during mouse skin carcinogenesis are presented.

초유 유청 분획의 Mouse Splenocyte 증식 효과 (Effect of Bovine Colostrum Factions on the Proliferation of Mouse Splenocytes)

  • 하월규;원도희;양희진;황경아;이수원
    • 한국축산식품학회지
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    • 제25권2호
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    • pp.250-256
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    • 2005
  • To investigate the effect of bovine colostral whey fractions on in vitro proliferation of mouse splenocytes, polypeptide fractions were separated from acid whey into 3 fractions depending on molecular weight by ultrafiltration: Fraction I, which contains the polypeptide larger than 10,000 Da, Fraction n, which contains the polypeptide ranging from 1,000 Da to 10,000 Da and Fraction III, which contains the polypeptide smaller than 1,000 Da. Fraction II showed the highest proliferative effect of mouse splenocytes among the colostral whey fractions and this proliferative activity increased in dose dependent manner. Unheated Fraction II and Fraction III showed significantly (p<0.01) higher proliferative effects than others but heated Fraction II showed the highest enhancing effect of mouse splenocyte among heated whey fractions (p<0.01). The supplementation of Fraction II and Fraction m showed greater proliferative effect of mouse splenocytes stimulated by concanavalin A (Con A) than that of whole whey or Fraction L Proliferative effect of mouse splenocytes stimulated by phytohemagglutinin (PHA) was the highest when Fraction II was supplemented Proliferative effect of the colostral whey fractions on mouse splenocytes by stimulation of lipopolysaccharide (LPS) was markedly enhanced by supplementation of Fraction II and Fraction m compared with whole whey and Fraction L It was estimated that colostral whey fraction containing IGF-I positively affected proliferation of mouse splenocyte.

다양한 유리화 동결 방법이 각 시간대별 생쥐 전핵기 배아의 발달에 미치는 영향 (The Effect of Various Vitrification Methods on Developmental Rate of Mouse Pronuclear Embryos at Different Recovery Times)

  • 김지철;서병부;박성백;김재명
    • 한국수정란이식학회지
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    • 제27권1호
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    • pp.63-69
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    • 2012
  • The objective of this study was to investigate the effectiveness of cryopreservation methods for the effect of various vitrification containers, such as EM-grid, OPS, or cryo-loop on the survival and developmental rate of vitrified mouse pronuclear embryos, and mouse cleavage embryo, at 21, 24, 27 and 30 hr after hCG injection. Post-thaw cleavage was similar among treatments, while the developmental rates of mouse blastocyst and hatched blastocyst were higher ($p$ <0.05) in 27 hr and 30 hr than 21 hr. The developmental rate of hatched blastocyst at vitrified cleavage mouse embryos in cryo-loop was significantly higher than vitrified pronuclear embryos of control group as well as EM-grid and OPS ($p$ <0.05). The developmental rate using cryo-loop was higher than EM-grid, but in case of OPS at vitrified cleavage and mouse pronuclear embryos, no significant difference was noticed. These results of our study show that the developmental rates of mouse embryos were unaffected by various vitrification containers, but in case of mouse embryos and hatched blastocysts at late vitrified pronuclear embryos the developmental rates were higher than early vitrified pronuclear embryos. Moreover, the developmental rate of hatched blastocyst at vitrified cleavage mouse embryos was significantly higher than vitrified pronuclear embryos. For better execution of this study, it will be mandatory to include improvement of vitrification containers, cryopreservation methods and conditions, higher survival rate, safe preservation, contamination and embryo loss.

양혈제습탕(凉血除濕湯)이 아토피 피부염 유발 NC/Nga mouse의 비장 및 DLN내 면역 관련 인자에 미치는 영향 (Effect of Yanghyeuljeseuptang on immunological factors in spleen and draining lymph node(DLN) of atopic dermatitis induced NC/Nga mouse by dinitrochlorobenzene(DNCB))

  • 박두병;한재경;김윤희
    • 혜화의학회지
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    • 제16권2호
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    • pp.251-265
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    • 2007
  • Yanhyeoljeseuptang(YHJST) is a traditional herbal medicine used for the treatment of dermatitis. The aim of this study was to confirm whether or not YHJST has a preventive effect on development of atopic dermatitis in dinitrochlorobenzene(DNCB)-applied Nc/Nga mouse. This study was undertaken to develop a reliable mouse model demonstrating similar immunologic phenomena as human atopic dermatitis characterized with predominance of type-2 immune response. NC/Nga mouse were sensitized with $200\;{\mu\ell}$ of 1% 2,4-dinitrochlorobenzene(DNCB) (acetone : olive oil = 3 : 1 mixture) and challenged twice or three times with $150\;{\mu\ell}$ of 0.2% DNCB in a week for the following 4 weeks. YHJST was administered orally to Nc/Nga mouse for 8 weeks, which led to the remarkable suppression on the development of dermatitis, as determined by various immune factors related to pathogenesis of atopic dermatitis in splenocytes and DLN cells. In this study, YHJST selectively suppressed T ce11 (CD4+, CD3+/CD69+, CD4+/CD25+) activation, which may be essential for ratio of IL-4 versus INF-$\gamma$ produced in the splenic T cell culture supernatants was approximately 3-fold higher in the mouse treated with DNCB than their control mouse respectively. Immunologic studies showed down-regulated that the capacity of spleen T cells to produce IL-4, but IFN-$\gamma$ was up-regulated by means of oral intake of these YHJST. These results strongly suggest that YHJST is a promising candidate for treatment of human atopic dermatitis.

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구풍제습탕(驅風除濕湯)이 아토피 피부염 유발 NC/Nga mouse의 비장 및 DLN내 면역 관련 인자에 미치는 영향 (Effect of Gupoongjeseuptang on immunological factors in spleen and draining lymph node(DLN) of atopic dermatitis induced NC/Nga mouse by dinitrochlorobenzene(DNCB))

  • 윤재은;한재경;김윤희
    • 혜화의학회지
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    • 제16권2호
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    • pp.267-280
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    • 2007
  • Gupoongjeseuptang(GPJST) is a traditional herbal medicine used for the treatment of dermatitis. The aim of this study was to confirm whether or not GPJST has a preventive effect on development of atopic dermatitis in dinitrochlorobenzene(DNCB)-applied Nc/Nga mouse. This study was undertaken to develop a reliable mouse model demonstrating similar immunologic phenomena as human atopic dermatitis characterized with predominance of type-2 immune response. NC/Nga mouse were sensitized with $200\;{\mu\ell}$ of 1% 2,4-dinitrochlorobenzene(DNCB) (acetone : olive oil = 3 : 1 mixture) and challenged twice or three times with $150\;{\mu\ell}$ of 0.2% DNCB in a week for the following 4 weeks. GPJST was administered orally to Nc/Nga mouse for 6 weeks, which led to the remarkable suppression on the development of dermatitis, as determined by various immune factors related to pathogenesis of atopic dermatitis in splenocytes and DLN cells. In this study, GPJST selectively suppressed T ce11 (CD4+, CD3+CD69+, CD4+CD25+) activation, which may be essential for ratio of IL-4 versus INF-$\gamma$ produced in the splenic T cell culture supernatants was approximately 3-fold higher in the mouse treated with DNCB than their control mouse respectively. Immunologic studies showed down-regulated that the capacity of spleen T cells to produce IL-4, but IFN-$\gamma$ was up-regulated by means of oral intake of these GPJST. These results strongly suggest that GPJST is a promising candidate for treatment of human atopic dermatitis.

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광섬유 소산파를 이용한 면역 센서 제조 및 그 특성 (Fabrication of fiber-optic evanescent wave immunosensor and its measuring characteristics)

  • 최기봉;윤희주;차승희;최정도
    • 센서학회지
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    • 제6권5호
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    • pp.356-361
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    • 1997
  • 광섬유 소산파 센서(fiber-optic evanescent wave sensor)를 제작하였다. 클래드층을 제거한 광섬유 코아 표면에 anti-mouse immunoglobulin G(IgG)를 결합시키고, 형광이 표지된 mouse IgG와의 반응을 직접적인 방법과 경쟁적인 방법을 통하여 측정하였다. 직접적인 방법과 경쟁적인 방법 모두 $1{\mu}g/m{\ell}$이하의 mouse IgG를 측정할 수 있는 감도를 얻을 수 있었다. Anti-mouse IgG는 단순 흡착 방법에 의하여 광섬유 코아 표면의 93.9%에 고정되었고 비특이적 결합반응을 제거하기 위하여 실시한 소혈청 알부민(bovine serum albumin : BSA)을 이용한 표면 코팅 효과는 없었다. Mouse IgG에 결합된 fluorescein의 비율이 높을수록 형광 발생량이 많았으나 관계는 직선적이지 않았다. 본 연구에서 제작된 광섬유 소산파 센서는 $1{\mu}g/m{\ell}$ 이하의 항원 항체 반응을 소산파 여기에 의한 형광량으로 측정할 수 있어 면역센서로의 응용이 가능할 것으로 판단된다.

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