• Animal cells and systems
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• v.1 no.2
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• pp.363-370
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• 1997

RNase MRP is a ribonucleoprotein complex with a site-specific endonuclease activity. Its original substrate for cleavage is the small mitochondrial RNA near the mitochondrial DNA replication origin, thus it was proposed to generate the primer for mtDNA replication. Recently, it has been shown to have another substrate in the nucleus, such as pre-S.8S ribosomal RNA in nucleolus. The gene for the RNA component of RNase MRP (MRP RNA) was found to be encoded by the nucleus genome, suggesting an interesting intracellular trafficking of MRP RNA to both mitochondria and nucleolus after transcription in nucleus. In this study, genomic DNA encoding MRP RNA was microinjected into the nucleus of Xenopus oocytes, to analyze promoter regions involved in the transcription. It showed that the proximal sequence element and TATA box are important for basal level transcription; octamer motif and Sp1 binding sites are for elevated level transcription. Most of Xenopus MRP RNA was exported out to the cytoplasm following transcription in the nucleus. Utilizing various hybrid constructs, export of MRP RNA was found to be regulated by the promoter and the 5' half of the coding region of the gene. Interestingly, the transcription in nucleus seems to be coupled to the export of MRP RNA to cytoplasm. Intracellular transport of injected MRP RNA can be easily visualized by whole-mount in situ hybridization following microinjection; it also shows possible intra-nuclear sites for transcription and export of MRP RNA.

• Korean Management Science Review
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• v.5 no.2
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• pp.16-24
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• 1988

본 연구에서는 MRP시스템을 도입하여 현재 사용하고 있는 우리나라 제조업체들을 대상으로 MRP시스템의 도입 및 적용과정과 관련된 여러 상황을 조사하였다. 다시 말하면 MRP사용업체들의 현주소를 파악하는 것이 본 연구의 주된 목적이다. 시간의 제약 때문에 MRP사용업체들의 파악이 순조롭게 진행되지 않아서 대상기업들의 수효가 많지 않고, 시스템의 도입 역사가 일천한 관계로 본 연구는 MRP시스템의 적용과정에 대한 예비조사로 규정한다. 그리고 본 연구의 경험을 기반으로 차후에 보다 광범위하고 세밀한 연구가 이루어질 것으로 기대한다.

• Journal of Korean Society for Quality Management
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• v.13 no.1
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• pp.65-76
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• 1985

The purpose of this thesis is to develop a micro-computer application for GT/MRP (group technology/material requirement planning) integrated system for efficient management of production scheduling. GT and MRP system have been found to have several drawbacks in practice. By GT and MRP system, however, it should be able to construct a GT/MRP integrated system that possesses the advantages of both concepts while alleviating their individual limitations.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.2
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• pp.268-274
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• 2015

Makgeolli residue protein (MRP) was extracted from byproduct of makgeolli processing, and MRP films containing various plasticizers were prepared. Among the plasticizers used in this study, MRP film containing glycerol-sorbitol (1:2) showed the most desirable mechanical properties. In addition, MRP films containing wasabi extract (WE) were prepared by incorporating different amounts (0, 0.8, 1.0, and 1.2%) of WE into film-forming solution. Tensile strength, elongation at break, and moisture content of MRP films decreased with addition of WE as compared with the control. However, MRP films containing WE showed antimicrobial activities against Escherichia coli O157:H7 and Listeria monocytogenes. Application of MRP film containing 1.0% WE to beef packaging decreased populations of E. coli O157:H7 and L. monocytogenes after storage at $4^{\circ}C$ for 8 days by 1.1 and 0.41 log CFU/g, respectively, compared with those of the control. In addition, the peroxide value and 2-thiobarbituric acid reactive substance value decreased by 53 and 56%, respectively, compared to the control. Therefore, these results suggest that MRP film containing WE can be used to improve the quality of beef during storage.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.38 no.3
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• pp.243-248
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• 2010

This study addresses spacecraft moment of inertial estimation approach using Modified Rodrigues Parameters(MRP). The MRP offer advantage by avoiding singularity in Kalman Filter design for attitude determination caused by the norm constraint of quaternion parameters. Meanwhile, MRP may suffer singularity for large angular displacement, so that we designed appropriate reference attitude motion for accurate estimation. The proposed approach is expected to provide stable error covariance update with accurate spacecraft mass property estimation results.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.22 no.51
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• pp.117-127
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• 1999

Modern production control systems based on MRPⅡ (Manufacturing Resource planning) didn't solved about the capacity and lead time problems. In the past, It solved that problems under deterministic. Therefore, the problem about determination of correct lead time and finite capacity are studied continuously by researchers. Now, we need to a integrated method that is from low level to high level instead of top-down in MRP. In this research uses the MRP-C(Capacitated Material Requirement Planning) concept that is more efficient then MRP system for capacity and flexible lead time , and shows the MRP-C algorithm and the prototype.

• Journal of the Korea Safety Management & Science
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• v.3 no.3
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• pp.87-95
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• 2001

In Production system, there are two ; One is the JIT system, which was developed by the Toyota corporation : the other is the MRP system, which was developed in the United States. The JIT system has been proved its effectiveness for production management system by many industries, however the effectiveness of its application in non-repetitive productions is yet uncertain unlike the MRP system. There has been many efforts in comparing and analyzing the usefulness and the limitation of those production systems. Accordingly, this study has focused its simulation model using MRP and JIT.

• Korean Management Science Review
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• v.3
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• pp.62-70
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• 1986

본 고의 제2장에서는 생산시스템의 기본기능연관과 유형 및 관리 측면에서의 분석방법에 대해 설명하고, 제3장에서는 Honeywell에서 개발된 MRP(Manufacturing Resource Planning)응용 종합 생산관리 패키지인 HDMS(Honeywell Distributed Manufacturing System)를 중심으로 최신 폐쇄경로 MRP(Closed Loop MRP)개념 및 서브시스템에 대해서 설명하기로 한다.

• Elastomers and Composites
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• v.45 no.2
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• pp.106-111
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• 2010

Magneto-rheological elastomers (MREs) were manufactured by incorporation of magnetic responsible powder (MRP) into natural rubber and silicone rubber. The optimum loading amounts of MRP was 30 vol.% and the natural rubber based MRE (NR-MRE) showed better mechanical property than that of silicone rubber based MRE (S-MRE). However, the modulus shift ratio caused by S-MRE, measured by Self-modified Electromagnet Applied Fast Fourier Transform Analyser (SEFFTA), was higher than that of NR-MRE. The modulus shift ratio caused by NR-MRE was 10%, while the modulus shift ratio caused by S-MRE was 35.7%. The modulus shift ratio could be improved by orienting the magnetic direction of MRP before crosslinking the MRE. The degree of orientation of MRP was analyzed using SEM.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.24
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• pp.10597-10601
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• 2015

Up-regulation of multidrug resistance-associated protein 1 (MRP1) is regarded as one of the main causes for multidrug resistance (MDR) of tumor cells, leading to failure of chemotherapy-based treatment for a multitude of cancers. However, whether silencing the overexpressed MRP1 is sufficient to reverse MDR has yet to be validated. This study demonstrated that RNAi-based knockdown of MRP1 reversed the increased efflux ability and MDR efficiently. Two different short haipin RNAs (shRNAs) targeting MRP1 were designed and inserted into pSilence-2.1-neo. The shRNA recombinant plasmids were transfected into cis-dichlorodiamineplatinum-resistant A549 lung (A549/DDP) cells, and then shRNA expressing cell clones were collected and maintained. Real time PCR and immunofluorescence staining for MRP1 revealed a high silent efficiency of these two shRNAs. Functionally, shRNA-expressing cells showed increased rhodamine 123 retention in A549/DDP cells, indicating reduced efflux ability of tumor cells in the absence of MRP1. Consistently, MRP1-silent cells exhibited decreased resistance to 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and DDP, suggesting reversal of MDR in these tumor cells. Specifically, MRP1 knockdown increased the DDP-induced apoptosis of A549/DDP cells by increased trapping of their cell cycling in the G2 stage. Taken together, this study demonstrated that RNAi-based silencing of MRP1 is sufficient to reverse MDR in tumor cells, shedding light on possible novel clinical treatment of cancers.