• 제목/요약/키워드: multiple column curves

검색결과 21건 처리시간 0.025초

중심압축하중을 받는 스테인리스 강관 기둥의 좌굴내력에 관한 연구 (A Study on the Buckling Strength of Centrally Compressed Stainless Steel Tubular Columns)

  • 장호주;양영성
    • 한국강구조학회 논문집
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    • 제17권2호통권75호
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    • pp.207-216
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    • 2005
  • 본 논문은 스테인리스 각형강관 및 원형강관에 대한 좌굴내력에 관한 연구로서 건축구조용 강재로서의 적용성 검토를 위해, 세장비를 주요 변수로 한 소재의 인장강도실험과 stub-column의 압축강도실험, 기둥의 중심압축실험을 실시하여 강재의 기계적 성질과 기둥의 강도 및 거동을 파악한다. 또한 이론해석과 각국 기준식(AIK-LSD, AISC-LRFD, AIJ-LSD, SIJ-ASD) 및 복수강도곡선의 적용을 통한 이론값과 실험값을 비교함으로서 건축구조용 강재로서 적용성 검토와 구조설계기준 확립을 위한 기초 자료를 구하는데 목적이 있다.

Methylisothiocyanate를 이용한 아미노산 배열결정시 N(O)-butyldimethylsilyl 유도체로서의 methylthiohydantoin 아미노산의 기체 크로마토그래피에 의한 분석 (Gas-chromatographic determination of methylthiohydantoin amino acid as N(O)-butyldimethylsilyl derivatives in amino acid sequencing with methylisothiocyanate)

  • 우강융
    • Applied Biological Chemistry
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    • 제35권2호
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    • pp.132-138
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    • 1992
  • Methylisothiocyanate에 의한 단백질의 아미노산 배열 결정시 순차적으로 분리되어 나오는 methylthiohydantion 아미노산을 기체 크로마토그라피로 효과적으로 정성 및 정량하기위하여 새로운 silylating reagent인 N-methyl-N-(tert.-butyldimethylsily)trifluoroacetamide를 사용하여 N-tert.-butyldimethylsily MTH 유도체로 silylation 한 후 HP-1 capillary column으로 분석하였다. Cystine을 제외한 21개의 단백질 구성 아미노산을 동정할 수 있었고 지금까지 packed column에서 TMS 유도체로 동정할 수 없었던 arginine도 분리 동정되었다. 2개 이상의 peak를 나타낸 것으로는 hydroxyproline, proline, isoleucine, glycine 및 tyrosine이었고 이중 hydroxyproline은 많은 수의 peak들로 분리되었다. Lysine, histidine 및 arginine은 주입량 $5.0\;nmole{\sim}15.0\;nmole$의 범위에서 나머지는 $2.5\;nmole{\sim}7.5\;nmole$의 범위에서 상관관계를 측정한 결과 고도의 직선 상관관계를 나타내었다(p<0.001). TMS 유도체에 의한 분석은 많은 불순 peak들 때문에 정량분석에 이용할 수 없었다.

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On successive machine learning process for predicting strength and displacement of rectangular reinforced concrete columns subjected to cyclic loading

  • Bu-seog Ju;Shinyoung Kwag;Sangwoo Lee
    • Computers and Concrete
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    • 제32권5호
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    • pp.513-525
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    • 2023
  • Recently, research on predicting the behavior of reinforced concrete (RC) columns using machine learning methods has been actively conducted. However, most studies have focused on predicting the ultimate strength of RC columns using a regression algorithm. Therefore, this study develops a successive machine learning process for predicting multiple nonlinear behaviors of rectangular RC columns. This process consists of three stages: single machine learning, bagging ensemble, and stacking ensemble. In the case of strength prediction, sufficient prediction accuracy is confirmed even in the first stage. In the case of displacement, although sufficient accuracy is not achieved in the first and second stages, the stacking ensemble model in the third stage performs better than the machine learning models in the first and second stages. In addition, the performance of the final prediction models is verified by comparing the backbone curves and hysteresis loops obtained from predicted outputs with actual experimental data.

Study of a self-centering beam-column joint with installed tapered steel plate links

  • Liusheng He;Yangchao Ru;Haifeng Bu;Ming Li
    • Structural Engineering and Mechanics
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    • 제87권4호
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    • pp.391-403
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    • 2023
  • In this study, a new type of self-centering beam-column joint with tapered steel plate links is proposed. Firstly, mechanical property of the basic joint (with the prestressed steel strands only, to provide the self-centering ability) and the combined joint (with both the prestressed steel strands and tapered steel plate links, to provide self-centering and energy dissipation simultaneously) is theoretically analyzed. Then, three joints with different dimensions and combinations of tapered plate links are designed and tested through a series of quasi-static cyclic loading tests. Test results show that a nearly bilinear elastic moment-rotation relationship for the basic joint is obtained. With the addition of tapered steel plate links, typical flag-shape hysteretic curves are obtained, which indicates good self-centering and energy dissipating ability of the combined joint. By installing multiple tapered plate links, stiffness and bearing capacity of the beam-column joint can be enhanced. The theoretical moment-rotation relationships agree well with the test results. A simplified macro model of the proposed joint is developed using OpenSees, which simulates reasonably well its hysteretic behavior.

Rapid Determination of Ginkgolic Acids in Ginkgo biloba Leaf Using Online Column Switching High-Performance Liquid Chromatography-Diode Array Detection and Confirmation by Liquid Chromatography-tandem Mass Spectrometry

  • Lee, Hyounyoung;Lim, Heungyoul;Yang, Juhong;Hong, Jongki
    • Bulletin of the Korean Chemical Society
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    • 제34권12호
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    • pp.3629-3634
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    • 2013
  • In this study, an improved method for the quantitative analysis of ginkgolic acids (GAs) in Ginkgo biloba leaf extract was developed. The samples were extracted with a mixture of chloroform and 50 % ethanol, after which the chloroform extract was dried and reconstituted in methanol. GAs with 13:0, 15:1, and 17:1 in the extract were successfully separated within 40 min and determined with high throughput performance using an online column-switching HPLC method using an SP column C8 SG80 ($4.6{\times}150mm$, $5{\mu}m$) and a Cadenza 5CD C18 column ($4.6{\times}150mm$, $3{\mu}m$). The developed HPLC method was validated for Ginkgo biloba leaf extract. The validation parameters were specificity, linearity, precision, accuracy, and limits of detection and quantitation (LODs and LOQs, respectively). It was found that all of the calibration curves showed good linearity ($r^2$ > 0.9993) within the tested ranges. The LODs and LOQs were all lower than $0.04{\mu}g/mL$. The established method was found to be simple, rapid, and high throughput for the quantitative analysis of GAs in ten commercial Ginkgo biloba leaf extract and dietary supplements. The samples were also analyzed in LC-electrospray ionization (ESI) tandem mass spectrometry (MS/MS) - multiple-ion reaction monitoring (MRM) mode to confirm the identification results that were obtained by the column switching HPLC-DAD method. The developed method is considered to be suitable for the routine quality control and safety assurance of Ginkgo biloba leaf extract.

Data Base에 의한 CFT 기둥의 내력에 관한 연구 (A Study on the Strength of Concrete Filled Tubular Columns according to Data-Base)

  • 서정환;양영성
    • 한국강구조학회 논문집
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    • 제13권1호
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    • pp.71-79
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    • 2001
  • 본 연구는 콘크리트 충전강관을 고층 건축구조물의 기둥부재로의 이용을 위한 연구의 일환으로 필자의 실험결과와 그동안 국내에서 발표된 콘크리트충전 강관 기둥에 관한 연구논문(1991.1~2000.6)의 실험결과 값을 데이터 베이스를 통해 콘크리트 충전으로 인한 내력 상승효과와 세장비에 따른 내력 등을 파악하여 다음과 같은 결론을 얻었다. 콘크리트 충전 강관 단주의 콘크리트 충전으로 인한 내력 상승효과는 단면형태에 따라 달리 나타나며, 누가강도에 비해 충전각형 강관은 1.102배 충전원형강관은 1.279배의 내력상승효과를 나타냈다. 압축력과 휨을 받는 콘크리트 충전강관의 폭-두께비는 국내의 비충전 강관의 폭-두께비에 비해 충전각형관은 1.3배 충전원형강관은 1.6배 이상 완화할 수 있음을 알 수있었다. 충전 효과를 고려한 중심축 하중을 받는 콘크리트 충전 강관 기둥의 내력식을 제안하였다. 실험데이트를 대표적인 복수강도 곡선인 SSRC 곡선 및 ECCS 곡선과 비교하였다.

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LC-ESI-MS/MS를 이용한 계지탕 중 주요 성분 분석 (Quantitative Determination of the Bioactive Marker Components in Gyeji-tang Using LC-ESI-MS/MS)

  • 서창섭;하혜경
    • 생약학회지
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    • 제49권1호
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    • pp.76-83
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    • 2018
  • A traditional herbal formula, Gyeji-tang has been used to treat the early colds, headache, chills, and fever in Asian countries. In this study, we were performed simultaneous determination of the 14 bioactive marker compounds, gallic acid, spinosin, paeoniflorin, albiflorin, liquiritin apioside, liquiritin, 6'''-feruloylspinosin, liquilitigenin, coumarin, cinnmamic acid, benzoylpaeoniflorin, cinnamaldehyde, glycyrrhizin, and 6-gingerol in Gyeji-tang using an ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS/MS). Analytical column was used a Waters Acquity UPLC BEH $C_{18}$ analytical column ($2.1{\times}100mm$, $1.7{\mu}m$) and maintained at $45^{\circ}C$ with a flow rate of 0.3 mL/min. The mobile phase consists of 0.1% (v/v) formic acid in water and acetonitrile with gradient elution. The MS analysis was conducted using multiple reaction monitoring in the positive and negative modes by a Waters ACQUITY TQD LC-MS/MS system. The calibration curves of 14 bioactive marker compounds showed linearity with correlation coefficients ${\geq}0.9798$. The limits of detection and quantification values were in the range of 0.11-6.66 ng/mL and 0.34-19.99 ng/mL, respectively. As a result of the analysis using the established LC-MS/MS method, the amounts of tested 14 compounds in the lyophilized Gyeji-tang sample were detected up to $85.7{\mu}g/g$. These results may be useful for quality assessment of a traditional herbal formulas.

Development and validation of LC-MS/MS for bioanalysis of hydroxychloroquine in human whole blood

  • Park, Jung Youl;Song, Hyun Ho;Kwon, Young Ee;Kim, Seo Jin;Jang, Sukil;Joo, Seong Soo
    • Journal of Biomedical and Translational Research
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    • 제19권4호
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    • pp.130-139
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    • 2018
  • This study aimed to analyze a high-performance liquid chromatography (HPLC) separation using a pentafluorophenyl column of parent drug hydroxychloroquine (HCQ) and its active metabolite, desethylhydroxchloroquine (DHCQ) applying to determine bioequivalence of two different formulations administered to patients. A rapid, simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for bioanalysis of HCQ and its metabolite DHCQ in human whole blood using deuterium derivative $hydroxychloroquine-D_4$ as an internal standard (IS). A triple-quadrupole mass spectrometer was operated using electrospray ionization in multiple reaction monitoring (MRM) mode. Sample preparation involves a two-step precipitation of protein techniques. The removed protein blood samples were chromatographed on a pentafluorophenyl (PFP) column ($50mm{\times}4.6mm$, $2.6{\mu}m$) with a mobile phase (ammonium formate solution containing dilute formic acid) in an isocratic mode at a flow rate of 0.45 mL/min. The standard curves were found to be linear in the range of 2 - 500 ng/mL for HCQ; 2 - 2,000 ng/mL for DHCQ in spite of lacking a highly sensitive MS spectrometry system. Results of intra- and inter-day precision and accuracy were within acceptable limits. A run time of 2.2 min for HCQ and 2.03 min for DHCQ in blood sample facilitated the analysis of more than 300 human whole blood samples per day. Taken together, we concluded that the assay developed herein represents a highly qualified technology for the quantification of HCQ in human whole blood for a parallel design bioequivalence study in a healthy male.

LC-ESI-MS에 의한 사군자탕의 지표성분 분석 (Analysis of the Marker Compounds in Sagunja-tang by LC-ESI-MS)

  • 서창섭;신현규
    • 생약학회지
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    • 제50권1호
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    • pp.65-71
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    • 2019
  • One of the oriental medicine prescriptions, Sagunja-tang consists of four herbal medicines (Ginseng Radix, Poria Sclerotium, Atractylodis Rhizoma Alba, and Glycyrrhiziae Radix et Rhizoma) and has been used as a medicine to enhance tonify the function of spleen and stomach in Korea. In this study, we conducted simultaneous analysis of the 9 marker components, liquiritin apioside, liquiritin, ginsenoside Rg1, liquiritigenin, ginsenoside Rb1, glycyrrhizin, atractylenolide III, atractylenolide II, and atractylenolide I in Sagunja-tang using a liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS). Marker compounds were separated on a Waters Acquity UPLC BEH $C_{18}$ analytical column ($2.1{\times}100mm$, 1.7 mm) and the column was maintained at $45^{\circ}C$. The mobile phase consists of 0.1% (v/v) aqueous formic acid and acetonitrile with gradient condition. The LC-MS analysis was performed using a Waters ACQUITY TQD LC-MS/MS system with multiple reaction monitoring (MRM) method in the positive and negative modes. The calibration curves of the nine marker components showed good linearity with coefficient of determination ${\geq}0.9984$ within tested range. The limits of detection and limits of quantification values were 0.27-2.42 ng/mL and 0.81-7.27 ng/mL, respectively. The concentrations of tested 9 analytes in the lyophilized Sagunja-tang sample using the established LC-ESI-MS/MS MRM method were detected up to 16.593 mg/g. These results can be useful as a basic data for the quality control of an oriental medicine prescriptions.

Development and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of ε-Acetamidocaproic Acid in Rat Plasma

  • Kim, Tae Hyun;Choi, Yong Seok;Choi, Young Hee;Kim, Yoon Gyoon
    • Toxicological Research
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    • 제29권3호
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    • pp.203-209
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    • 2013
  • A simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of ${\varepsilon}$-acetamidocaproic acid (AACA), the primary metabolite of zinc acexamate (ZAC), in rat plasma by using normetanephrine as an internal standard. Sample preparation involved protein precipitation using methanol. Separation was achieved on a Gemini-NX $C_{18}$ column ($150mm{\times}2.0mm$, i.d., 3 ${\mu}m$ particle size) using a mixture of 0.1% formic acid-water : acetonitrile (80 : 20, v/v) as the mobile phase at a flow rate of 200 ${\mu}l/min$. Quantification was performed on a triple quadrupole mass spectrometer employing electrospray ionization and operating in multiple reaction monitoring (MRM) and positive ion mode. The total chromatographic run time was 4.0 min, and the calibration curves of AACA were linear over the concentration range of 20~5000 ng/ml in rat plasma. The coefficient of variation and relative error at four QC levels were ranged from 1.0% to 5.8% and from -8.4% to 6.6%, respectively. The present method was successfully applied for estimating the pharmacokinetic parameters of AACA following intravenous or oral administration of ZAC to rats.