• 제목, 요약, 키워드: mycelia extracts

검색결과 85건 처리시간 0.05초

Radical Scavenging Activities of Undaria pinnatifida Extracts Fermented with Cordyceps militaris Mycelia

  • Kim, Yon-Suk;Kim, Eun-Kyung;Hwang, Jin-Woo;Han, Young-Ki;Kim, Seong-Eun;Jeong, Jae-Hyun;Moon, Sang-Ho;Jeon, Byong-Tae;Park, Pyo-Jam
    • Journal of Microbiology and Biotechnology
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    • v.25 no.6
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    • pp.820-827
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    • 2015
  • The present study was performed to investigate the various radical scavenging activities of fermented Undaria pinnatifida by the mycelia fermentation method. U. pinnatifida was fermented with Cordyceps militaris (C. militaris ) mycelia using solid culture and compared with unfermentated U. pinnatifida and C. militaris mycelia for antioxidant activities. The various radical scavenging activities of extracts from U. pinnatifida fermented with C. militaris mycelia (FUCM) were evaluated by electron spin resonance. The antioxidant activities of the FUCM extracts were assayed for ferric reducing antioxidant power, 2,2'-azinobis-(3-ethybenzothiazoline-6-sulfonic acid) radical scavenging activity, and oxygen radical absorption capacity. The free radical scavenging activity of FUCM extracts was higher than that of C. militaris mycelia or U. pinnatifida alone. FUCM extracts were significantly (p < 0.05) increased up to 35 times, 10 times, and 16 times that of U. pinnatifida extracts on DPPH, alkyl, and hydroxyl radical scavenging activities, respectively. These results indicate that FUCM extracts have different chemical ingredients from U. pinnatifida and could provide beneficial antioxidant activity.

상황버섯 균사체를 이용한 양갱 제조 및 품질 특성 (Quality Characteristics of Yanggaeng Supplemented with Sanghwang Mushroom (Phellinus linteus) Mycelia)

  • 홍성수;정은경;김애정
    • 대한영양사협회학술지
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    • v.19 no.3
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    • pp.253-264
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    • 2013
  • The principal objective of this study was to examine the quality characteristics of yanggaeng supplemented with powder derived from Sanghwang mushroom (Phellinus linteus) mycelia. We analyzed the potential of utilizing Phellinus linteus mycelia as a functional food material by estimating total polyphenol and flavonoid contents, electron-donating abilities, as well as antioxidative activities of the water and ethanol extracts of Sanghwang mushroom mycelia. The total phenol and flavonoid contents of ethanol extracts from Phellinus linteus mycelia were 0.69 mg/ml and 0.16 mg/ml, respectively, while the contents from the water extract of Phellinus linteus mycelia were 0.66 mg/ml and 0.22 mg/ml, respectively. The electron-donating abilities of ethanol and water extracts from Phellinus linteus mycelia were 88.64 and 90.29%, respectively. The ABTS radical scavenging activities of ethanol and water extracts from Phellinus linteus mycelia were 89.74 and 71.35%, respectively. In terms of color values, as the level of powder increased, the value of L (lightness) decreased, whereas those of a (redness) and b (yellowness) increased. In regard to the mechanical properties of the samples, we noted significant differences in hardness, springiness, chewiness, and gumminess (P<0.05). The results of the sensory evaluation showed that the score from SHM (Sanghwang mushroom mycelia) with 4% powder was significantly higher than other samples in terms of sweetness, color, taste, texture and overall quality (P<0.05). Taken together, the recommended level of Phellinus linteus mycelia powder in yanggaeng is 4% for optimal sensory characteristics.

상황버섯 균사체로 발효한 다시마 추출물의 항산화 활성 (Fermented Saccharina japonica with Phellinus linteus Mycelia Enhances its Radical Scavenging Activities)

  • 김연숙;박표잠;정재현
    • 한국수산과학회지
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    • v.50 no.4
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    • pp.359-365
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    • 2017
  • To develop new functional materials, Saccharina japonica (S. japonica) was cultivated with Phellinus linteus (P. linteus) mycelia by solid culture. S. japonica was fermented with P. linteus mycelia. The various radical scavenging activities of extracts of fermented S. japonica with P. linteus mycelia (FSPM) were investigated and compared to the antioxidant capabilities of unfermented S. japonica and P. linteus mycelia. The antioxidant activities of FSPM extracts were evaluated using ferric reducing antioxidant power (FRAP) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity. FSPM showed stronger free radical scavenging abilities than P. linteus mycelia or S. japonica alone. These results indicate that fermentation of S. japonica changes its chemical nature and could provide beneficial antioxidant effects.

인삼박으로 배양된 버섯 균사체 추출물이 암세포 증식에 미치는 영향 (Effect of Mycelia Extracts of Mushroom-Cultured Ginseng By-product on Proliferation in Cancer Cell Lines)

  • 박은미;김수정;예은주;배만종;조경철
    • 한국식품영양과학회지
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    • v.34 no.3
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    • pp.323-329
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    • 2005
  • 인삼박의 가용성 추출물을 용출시킨 후 남은 잔여 묵인 인삼박에 상황버섯, 영지버섯, 운지버섯, 표고버섯 균사체를 접종ㆍ배양하여 얻어진 인삼박 균사체 추출액의 전자공여능 및 간암세포(Hep3B)와 고형암(S-180)의 증식에 미치는 영향을 조사하였다. 인삼박 균사체의 전자공여능은 500-10,000ppm 범위에서 농도가 진할수록 높게 나타났으며 운지가 4종류의 균사체 추출물 중 가장 높았다. 간암세포의 형태변화 및 증식 억 제 에 미 치 는 영 향은 에 탄을 추출물이 물 추출물보다 효과적이었으며, 60% 에탄을 추출물 5,000 ppm에서 상황, 영지,운지에 비해 표고가 99.1%로 가장 강한 세포증식억 제 효과를 나타내었다. S-180 고형 암 억제효과는 대조군에 비해 운지가 65%로 가장 컸으며 영지 61%, 표고 56%, 상황 59%의 고형암 억제효과를 나타내었다. 따라서 인삼박을 이용하여 배양된 버섯 균사체가 간암세포와 고형암의 증식 억제에 효과적인 것으로 관찰되므로써 항암물질의 자원으로서 그 활용성이 기대된다고 하겠다.

Antioxidant, anti-acetylcholinesterase and xanthine oxidase inhibitory activities of three extracts from Phellinus igniarius

  • Jin, Ga-Heon;Lee, Min Woong;Im, Kyung Hoan;Lee, Tae Soo
    • 한국버섯학회지
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    • v.12 no.1
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    • pp.1-7
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    • 2014
  • This study was initiated to investigate antioxidant, anti-acetylcholinesterase, and xanthine oxidase inhibitory activities and properties of fruiting bodies, mycelia, and fermentation culture filtrates from Phellinus igniarius. The contents of total phenols and flavonoid of fruit bodies, mycelia, and culture filtrate were 15.35-1.36 mg/g, 10.35-7.85 mg/g, and 8.25-5.36 mg/g. The 1,1-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging abilities of the extracts from the fruiting bodies, mycelia, and culture filtrates were 90.25-95.60%, 78.82-85.24%, and 76.32-82.50% at $50-400{\mu}g/mL$, respectively. The chelating ability of fruiting body extract on ferrous ions was higher than those of mycelia and culture filtrates tested. The anti-acetylcholinesterase inhibitory activity of the fruiting body extract at 400 ${mu}g/mg$ exhibited 91.10% on AChE, which is lower than that of positive control, galanthamine (94.82%). The xanthine oxidase inhibitory activities of the fruiting bodies, mycelia, and culture extract were 85.47%, 78.13%, and 72.49% at 400 ${\mu}g/mL$, respectively. Overall, the fruiting body extract has better anti-acetylcholinesterase, antioxidant and xanthine oxidase inhibitory activities than those from mycelia and culture filtrate.

Anti-oxidization Effect of Extracts from Oriental Medicine and Cereal Medium Where Tricholoma matsutake Mycelia were Cultured

  • Kim, Hae-Ja;Lee, Ki-Nam
    • 동의생리병리학회지
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    • v.22 no.3
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    • pp.672-677
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    • 2008
  • In order to explore the anti-oxidization effect of oriental medicine and cereal medium(OCM) where Tricholoma matsutake mycelia were cultured, measurement of hot water extract and UMPM(extraction method using ultra sonic waves, micro waves, micro bubble) extract, the total polyphenol content of crude polysaccharide from each extract, SOD-like activity, electron donating ability(EDA), xanthine oxidase inhibitory activity, and tyrosinase inhibitory activity was conducted. The total polyphenol content of each extract was found to be 16.36% for hot water extract(HE) group and 15.73% for UMPM extract(UE) group and the amount of crude polysaccharide precipitated into ethanol of extracts was found to be 8.79% for UMPM ethanol extract(UEE) group and 6.48% for hot water ethanol extract(HEE) group. As a result of measurement of SOD-like activity by concentration of each extract, it was found to be 96.17% for UE group, 91.23% for HE group, 91.33% for UEE group, and 87.11% for HEE group at 20 mg/mL. In the case of EDA, it was found to be 47.55% for UE group, 44.93% for HE group, 25.38% for UEE group, and 18.36% for HEE group. And in the cases of the rates of xanthine oxidase inhibitory activity and tyrosinase inhibitory activity, as the concentration of each extract increased, the inhibition rate increased accordingly. As a result of comparison between hot water extract method and UMPM extract method using extracts obtained from oriental medicine compound medium where Tricholoma matsutake mycelia were cultured, all of the extracts were judged to have a high anti-oxidization effect. In particular, UMPM extracts were found to have higher polyphenol content, SOD-like activity, EDA, xanthine oxidase inhibitory activity and tyrosinase inhibitory activity compared to hot water extract method. In this regard, extracts obtained from OCM where Tricholoma matsutake mycelia were cultured are considered to have high availability as functional material when and if they are prepared using UMPM extract method.

먹물버섯 자실체 및 균사체 추출물의 돌연변이 억제효과 (Antimutagenic Effect of the fruiting Body and the Mycelia Extracts of Coprinus comatus)

  • 이갑랑;김현정;이병훈;김옥미;배준태;박선희;박동철
    • 한국식품영양과학회지
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    • v.28 no.2
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    • pp.452-457
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    • 1999
  • The inhibitory effect of Coprinus comatus on the mutagenicity in Salmonella assay system and SOS chromotest were studied. In Ames test, the ethanol and water extracts and the cultured mycelia fractions of Coprinus comatus did not show any mutagenicity, but the Coprinus comatus ethanol extracts showed inhibitory effects of 8 0∼90% on the mutagenicity induced by indirect mutagen of benzo(a)pyrene(B(a)P) and aflatoxin B1(AFB1) in Salmonella typhimurium TA98 and TA100. The antimutagenic effect increased with increasing concentration of the ethanol extract toward N methyl N' nitro N nitrosoguanidine(MNNG). However, the water extracts inhibited about 40∼50% against direct and indirect mutagen. The cultured mycelial filtrate of Coprinus comatus, the fractionⅡ, showed antimutagenic effect of 90% on MNNG and 25∼50% on B(a)P and AFB1. In SOS chromotest, the ethanol extracts of Coprinus comatus showed antimutagenic effect of 65∼81% on SOS function induced by 4 NQO, and the cultured mycelia fractionⅡ showed low inhibitory effect of 20∼50%.

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큰느타리버섯 균사체로 제조한 발효두부 추출물의 면역 활성 (Immuno-Activities of Extracts of Tofu Fermented with Pleurotus eryngii Mycelia)

  • 이상원;강종우;김재용;박경욱;박석규;주옥수;이성태;서권일
    • 한국식품영양과학회지
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    • v.39 no.1
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    • pp.25-30
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    • 2010
  • 두부의 기능성 및 저장성을 향상시킬 목적으로 큰느타리버섯 균사체를 이용한 발효두부를 제조하여 물과 메탄올로 추출하여 면역세포 활성에 미치는 효과를 조사하였다. 큰느타리버섯 균사체를 배양하기 위한 최적 배지는 PD broth 배지인 것을 확인하였으며, 큰타리버섯 균사체를 이용한 두부의 최적 발효기간은 7일 정도가 적당하였다. 큰느타리버섯 균사체를 이용하여 발효한 두부의 물 및 메탄올추출물은 $0.01 {\mu}g/mL$ 농도 이상에서 비장세포의 증식을 유도하였으며, 이들 추출물은 IL-6, IFN-$\gamma$ 분비를 유도하는 것으로 나타났다. 발효두부 물 추출물은 대조군에 비해 대식세포의 일산화질소 생산을 $1 {\mu}g/mL$ 농도 이상에서 유의적으로 증가시키는 것을 알 수 있었으며, 메탄올 추출물은 $10 {\mu}g/mL$ 농도 이상에서 그 생산을 증가시켰다. 발효두부 추출물들은 대식세포가 분비하는 IL-6, TNF-$\alpha$, IL-1$\beta$ 및 GM-CSF 분비량을 유의하게 증가시켰다. 따라서 큰느타리버섯 균사체로 발효한 두부는 기능성 두부로 개발이 가능하리라 생각된다.

3종의 버섯균사체로 발효한 천년초 에탄올 추출물의 생리활성 (Biological Activity of Ethanol Extracts from Fermented Opuntia humifusa with 3 Different Mushroom Mycelia)

  • 김미현
    • 한국식품영양학회지
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    • v.28 no.4
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    • pp.620-627
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    • 2015
  • The present study was carried out to investigate the biological activity of ethanol extracts from fermented Opuntia humifusa with 3 different mushroom mycelia: Phellinus linteus, Lentinula edodes, and Pleurotus ostreatus. Fermented Opuntia humifusa by Phellinus linteus (FOP) and Lentinula edodes (FOL) showed more DPPH and ABTS radical scavenging activities than non-fermented Opuntia humifusa (NFO) and fermented Opuntia humifusa by Pleurotus ostreatus (FOPO). At a concentration of 250 ppm, the ABTS radical scavenging activities of the FOP and FOL were similar to that of BHA, a synthetic antioxidant. The total polyphenol content had a similar tendency to that of the radical scavenging activity. However, the flavonoid content was increased in the order of NFO, FOL, FOPO, and FOP. At all concentrations, the tyrosinase inhibitory activity of FOP and FOPO were significantly higher than that of kojic acid. During adipocyte differentiation, NFO and FOL showed no significant difference in lipid accumulation in 3T3-L1 cells. FOP and FOPO showed a higher fat accumulation inhibitory effect than NFO and FOL. These results provide baseline data for Opuntia humifusa as a novel functional food.

동충하초(冬蟲夏草)의 항돌연변이(抗突然變異) 활성(活性)에 관한 연구(硏究) (A Study on Antimutagenic Activity of the Extracts from Paecilomyces japonica)

  • 박성호;정지천;서운교
    • 대한한방내과학회지
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    • v.21 no.2
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    • pp.309-318
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    • 2000
  • The fruiting bodies of Paecilomyces japonica been used for an anticancer and immuno-stimulating agent as an oriental medicine, Antimutagenecity test with SOS chromotest and antioxidant test with NBT method were carried out using the concentrated culture broth, the extract of mycelia, and that of fruiting bodies. Among the sample extracts tested, the extract of fruiting bodies was most effective to antimutagenecity against the mutagens tested such as MNNG, ethidium bromide(EtBr), 2-aminofluorene(AF) and nitrofluorene(NF). Antimutagenic activity of all the extracts were very effective against mutagen, MNNG. When the extracts were added to certain concentration, antimutagenic activity was enhanced against mutagen, MNNG and NF. Antioxidant activity of the extract from fruiting bodies was highest. However, its activity was very low, compared to ascorbic acid.

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