• Title, Summary, Keyword: mycelia extracts

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Development of Functional Food Materials from Acanthopanax senticosus-Fermented Mushroom Mycelia (가시오가피 버섯균사체 발효물의 기능성 식품 소재 개발)

  • Cho, Ju-Hyun;Choi, Goo-Hee;Park, In-Jae;Baik, Soon-Ok;Kim, Hyung-Ha;Kim, Choong-Sik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.3
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    • pp.411-418
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    • 2014
  • Three mushroom mycelia, Ganoderma lucidum, Hericium erinaceum, and Phellinus linteus, were separately diluted with the natural culture media Acanthopanax senticosus. Solid-state fermentation was used to produce three different A. senticosus-fermented mushroom mycelium groups: G. lucidum mycelia, H. erinaceum mycelia, and P. linteus mycelia. The resulting mycelia were analyzed to assess their efficacies as health functional foods. Optimized fermentation conditions were determined by considering the density and growth speed of mycelia in each A. senticosus-fermented mushroom mycelium group. The cultured mushroom mycelia under the optimized conditions were extracted using water and 70% ethanol. Extraction was followed by filtration, concentration and freeze-drying to produce extract powder of A. senticosus-fermented mushroom mycelia: Water extracts (FM-5111, FM-5121, and FM-5131) and 70% ethanol extracts (FM-5112, FM-5122, and FM-5132). Analysis of extract powder of A. senticosus-fermented mushroom mycelia was performed using the maker compounds eleutheroside B and eleutheroside E. Analysis of ${\beta}$-glucan contents was performed by enzymatic procedures.

Exploration of Functional Materials from Oriental Medicine Extracts Cultured with Tricholoma Matsutake Mycelium - (2) Effect of Extracts on Blood Glucose and Liver Function in Streptozotocin-Induced Diabetic Rat -

  • Kim, Hae-Ja;Choi, Yun-Hee;Cho, Hwa-Eun;Hong, Hak-Gi;Han, Jung-Ho;Lee, Ki-Nam
    • Journal of Society of Preventive Korean Medicine
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    • v.12 no.2
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    • pp.13-25
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    • 2008
  • The purpose of this study was to investigate extract from mixed culture with Trichloloma matsutake mycelium in oriental medicine and cereal medium(OCM) to develop new material for pharmaceutical products and medicinal food for diabetes mellitus. To evaluate of hypoglycemic activity of OCM extracts, we examined the inhibitory activity of ${\alpha}$-glucosidasein OCM, blood glucose level and liver function of streptozotocin(STZ) induced diabetic rat. Experimental group was divided into 6 groups: first, it was divided into normal control group(hereafter NC group) and diabetes-induced group, and diabetes-induced group was subdivided into diabetic control group(DC group), treated by hot water extracts group(HE), ultra sonic waves, micro waves, and micro bubble extracts g roup(UE), crude polysaccharide of HE group (HEE) and crude polysaccharide of UE group(UEE) at a dose of 300mg/kg/body weight, respectively. In diabetic-induced groups, after streptozotocin was melted in 0.01M citrate buffer at 50mg/kg/body weight, when the non-fasting blood glucose levelwas 300 mg/dl or more in blood collected from the tail vein, it was regarded as diabetic induction and then such diabetic-induced experimental animals were used in this experiment. At the end of the experiment, blood glucose level increased by 4.19% in DC group but significantly decreased by 32.34%, 19.19%, 17.81% and 17.64%, respectively in UEEE, UE, HE, and HEE groups. In the cases of AST, ALT, and ALP, the experiment group treated with extracts showed significantly lowerblood glucose level than DC group. The levels of BUN and uric acid were found to be lower in the UMPM extract group(UE) than HW extract group(HE), which implies that herb medicine medium extracts in which Tricholoma matsutake mycelia were cultured are effective in reducing impaired liver function as well as high blood glucose level caused by diabetes. In addition, the administration of low temperature UMPM extracts was found to produce better results than that of high temperature hot water extracts. In this regard, it is expected that extracts from herb medicine obtained by cultivating Tricholoma matsutake mycelia will be widely used as new ingredients for foods and medicines for prevention and treatment of diabetes.

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Isolation, Purification and Characterization of Polysaccharides that induce in vitro Immuno-Stimulation of Macrophases derived from Liquid Culture of Cordyceps militaris

  • Kwon, Jeong-Seok;An, Hyo-Sil;Hong, Eock-Kee
    • 한국생물공학회:학술대회논문집
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    • pp.287-292
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    • 2005
  • The crude polysaccharides(C-CPF, C-CPM, C-CPB) derived from fruiting body, mycelia and mycelia free broth of cordyceps militaris were obtained by ethanol precipitation of hot water extracts. After a batch fermentation of C. militaris was carried out in a 5 L jar vessel, endo-polysaccharide and exo-polysaccharide were obtained. They were demonstrated as the hetero polysaccharides which were composed of glucsose, galactose and mannose by performed with HPAEC(high pH anion exchange chromatography) and conformation of random coil by its complex forming ability with congo red reagent. They were purified by ion exchange (DEAE-cellulose) and gel filtration chromatography. They were monitered by phenol-sulfuric acid method and Bradford method. The NO induction activities of crude polysaccharides and purified polysaccharides derived from mycelia free broth were enhanced rather than LPS(lipo polysaccharide) which was used as a general NO inducer. These effects presumably contibute to the antitumor activities. The homogenieties and molecular weights of polysaccharides were determined by using Sepharose CL-6B. The yield, molecular weights and NO induction activities of C-CPFN Fr.III, C-CPMN Fr.III, C-CPBN Fr.II were 0.387, 0.408 and 0.153, 127 K 210 K and 36 K, 40.79%, 88.72%, and 104.17%, respectively.

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Purification and Characterization of Anti-complementary Polysaccharide from Phellinus linteus Mycelia (상황버섯(Phellinus linteus) 균사체로부터 항보체 활성 다당류의 정제 및 특성)

  • Seo, Ho-Chan
    • The Korean Journal of Mycology
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    • v.40 no.2
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    • pp.109-113
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    • 2012
  • We have isolated an anti-complementary polysaccharide from the hot water extracts of Phellinus linteus mycelia. Anti-complementary polysaccharide, PL-5-IIIa, was purified by ultrafiltration, gel permeation chromatography using Sepharose CL-4B. GPC (Sepharose CL-4B) and its homogenicity was demonstrated by HPLC. Using gel permeation chromatography with standard dextrans, its molecular weight was determined as about 800,000 dalton. The purified PL-5-IIIa was identified as a protein bound polysaccharide comprising of 29.6% protein and 64.2% carbohydrate which was composed of fucose(15.8%), galactose(43.1%) and mannose(40.6%).

Anti-Inflammatory Activities of Extracts from Fermented Taraxacum platycarpum D. Leaves Using Hericium erinaceum Mycelia (노루궁뎅이버섯 균사체로 발효한 민들레잎 추출물의 항염증 활성)

  • Kim, Yon-Suk;Joung, Mi-Yeun;Ryu, Beom-Seok;Park, Pyo-Jam;Jeong, Jae-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.1
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    • pp.20-26
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    • 2016
  • This study investigated the fermentation effect of Taraxacum platycarpum Dahlst. leaf extracts using Hericium ernaceum mycelia to test antioxidant and anti-inflammatory activities in vitro. The antioxidant activities of fermented or non-fermented extracts of T. platycarpum leaves were determined by 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, ferric reducing antioxidant power, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity. The leaf extract of T. platycarpum showed higher antioxidant activity than extract of fermented leaves. However, ethanolic extract of fermented T. platycarpum leaves decreased levels of nitric oxide production and pro-inflammatory cytokines such as interleukin-6 and tumor necrosis factor-${\alpha}$ in lipopolysaccharide-stimulated RAW 264.7 cells. Moreover, fermented leaf extract suppressed protein expression of inducible nitric oxide synthase in RAW 264.7 cell culture. Therefore, the enhanced anti-inflammatory activity of ethanolic extracts of fermented T. platycarpum leaves might be attributed to the molecular conversion of leaf ingredients during fermentation and the active ingredients might have specific affinity with ethanol during extraction.

Optimization for Production of Phellinus linteus Mycelia with Anti-Complement Activity (항보체 활성을 갖는 Phellinus linteus 균사체 생산의 최적화)

  • Seo, Ho-Chan
    • Journal of Applied Biological Chemistry
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    • v.53 no.3
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    • pp.179-183
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    • 2010
  • To produce the functional food materials from edible mushrooms, hot-water extracts from 70 kinds of mushroom mycelia were examined for anti-complementary activity and Phellinus linteus showed the highest activity through the complement fIxation test. The maximum production of Phellinus linteus mycelia with anti-complementary activity was observed in culture medium containing soluble starch 3.0%, peptone 0.3%, yeast extract 0.4%, $MgSO_4{\cdot}7H_2O$ 0.1%, $K_2HPO_4$ 0.2% and in the culture conditions controlled at initial pH 7.0, $30^{\circ}C$ and 150 rpm by the rotary shaker. In addition, the maximum production of mycelial dry weight was 15 mg/mL after 18 days under the optimal conditions, and anti-complementary activity was reached to 88% in 5 L-jar fermenter.

Studies on Submerged Culture and Mycelial Components of Naematoloma sublateritium Mycelia (개암버섯균의 액체배양과 균사체의 성분에 관한 연구)

  • Kang, An-Seok;Kang, Tae-Su;Cho, Soo-Muk;Yu, Seung-Hun
    • The Korean Journal of Mycology
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    • v.29 no.1
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    • pp.22-27
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    • 2001
  • This study was carried out to get the basic information for the submerged culture and analyze the biochemical components of Naematoloma sublateritium mycelia. The optimal temperature, pH, agitation speed and cultural time for the mycelial growth of Naematoloma sublateritium were $25^{\circ}C$, 5.5, 150rpm and 20 days, respectively. The proximate composition of mycelia was as follows; carbohydrate 55.8% (total sugar 48.7%), crude protein 22.4%, fat 4.1 % and ash 4.7% respectively. Among the free amino acid contents, phenylalanine, alanine and lysine were predominant component. The linoleic acid and palmitic acid were found to be the highest among the free fatty acids. The biopolymer extracts of mycelia was identified to be protein-bounded polysaccharide by color reaction and sepharose CL-4B gel chromatography.

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Hypoglycemic Effect of Extracts of Soybean Paste Containing Mycelia of Mushrooms in Streptozotocin- Induced Diabetic Rats (버섯된장 추출물의 당뇨 유발 흰쥐에 대한 혈당강하 효과)

  • Yang, Byung-Keun;Jeong, Sang-Chul;Hur, Nam-Jung;Ha, Sang-O;Kim, Ki-Young;Kym, Kyo-Hyeok;Yun, Jong-Won;Song, Chi-Hyun
    • The Korean Journal of Mycology
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    • v.28 no.2
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    • pp.126-129
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    • 2000
  • The hypoglycemic effects of the hot water extract of soybean paste containing mycelia of Phellinus linteus, Cordyceps militaris, Ganoderma lucidum were evaluated in streptozotocin-induced diabetic rats. All the diabetic groups were not significantly affected in food intake and food efficiency ratio. Significant decrease in blood glucose was observed in all the soybean paste containing mushroom mycelia (SPM) groups. Especially, administration of soybean paste containing mycelia of Ganoderma lucidum, blood glucose level was significantly lowered up to 45% compared to that of diabetic control group. The GPT and GOT activities indicated lower values in all the SPM groups than in the diabetic control group.

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Screening the Antibacterial Activities of Streptomyces Extracts against Phytopathogens Xanthomonas oryzae pathovar oryzae, Xanthomonas campestris pathovar vesicatoria, and Pectobacterium carotovorum pathovar carotovorum

  • Kim, Seung-Hwan;Cheng, Jinhua;Yang, Seung Hwan;Suh, Joo-Won;Song, Eun-Sung;Kang, Lin-Woo;Kim, Jeong-Gu
    • Journal of Applied Biological Chemistry
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    • v.58 no.3
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    • pp.253-258
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    • 2015
  • Xanthomonas oryzae pv. oryzae (Xoo), X. campestris pv. vesicatoria (Xcv), and Pectobacterium carotovorum pv. carotovorum (Pcc) are the causative agents of bacterial blight in rice, bacterial spot in pepper, and bacterial soft rot in carrot and cabbage, respectively. To isolate novel microbial extracts with antimicrobial activities against these bacteria, approximately 5,300 different Streptomyces extracts were prepared and tested. Microbial cultures from various Streptomyces strains isolated from the Jeju Island, Baekam, Mankyoung river, Jiri mountain etc. in Korea were extracted into three different factions -secreted hydrophobic, secreted hydrophilic, and mycelia- using ethyl acetate, water, and methanol. Initially, 34, 29, and 10 extracts were selected as having antibacterial activities against Xoo, Xcv, and Pcc, respectively. Extracts 1169G4, 1172E9, and 1172E10 had the highest growth inhibition activities against both Xoo and Xcv, and extracts 1151H7 and 1152H7 showed the highest growth inhibition activities against Pcc.