• Title, Summary, Keyword: mycelia extracts

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Optimization of Anticoagulant Production from Phellinus linteus Mycelia (상황버섯(Phellinus linteus) 균사체로부터 항혈전 생산의 최적화)

  • Seo, Ho-Chan
    • The Korean Journal of Mycology
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    • v.39 no.2
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    • pp.117-121
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    • 2011
  • To produce the functional food materials, 50 kinds of the mycelial extracts from edible mushroom were examined for anticoagulant activity and Phellinus linteus showed the highest activity through the activated partial thromboplastin test (aPTT). The maximum production of anticoagulant activity and the mycelial growth was observed in culture medium containing soluble starch 3.0%, peptone 0.1%, $MgSO_4{\cdot}7H_2O^{\circ}C$ 0.1%, $K_2HPO_4$ 0.1% and in the culture conditions controlled at initial pH 7.0, $30^{\circ}C$ and 150 rpm by the rotary shaker. In addition, the maximum production of mycelial dry weight was 7.5 mg/mL after 10 days under the optimal conditions, and anticoagulant activity was reached to 390 sec in 5 L-jar fermentor.

Physicochemical components of Astragalus membranaceus fermented with mushroom mycelia (담자균 균사체가 배양된 황기의 이화학적 성분분석)

  • Jang, Yeon-Jeong;Lee, Yun-Hye;Lee, Chan-Jung;Kim, Jae-Hyeon;Kim, Eun-Ju;Ji, Soo-Jeong;Park, Shin-Young
    • Korean Journal of Food Preservation
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    • v.23 no.1
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    • pp.49-56
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    • 2016
  • This study analyzed the physicochemical characteristics of Astragalus membranaceus (AM) fermented with seven different mushroom mycelia. Physicochemical characteristics, such as contents of moisture, pH, total reducing sugars, free sugar, and isoflavonoid, were investigated. The moisture content was increased in most of the samples. The pH values of AM fermented with Phellinus linteus and Flammulina velutipes were increased, while the pH of other samples were similar to that of non-fermented AM. The reducing sugar content was in the range of 211.69~391.74 mg/100 g. The extraction yield using water was higher than that when extracted with 80% ethanol. The free sugar content was increased through fermentation with mushroom mycelia. However, the glucose contents of the 80% ethanol and water extracts were decreased. Finally, the calycosin and formononetin contents in 80% ethanol and water extracts of AM fermented with Phellinus linteus were 2,549.24 mg/g, and 827.66 mg/g for calycosin, and 1,366.69 mg/g and 221.28 mg/g for formononetin, respectively. These results suggest that fermentation with mushroom mycelia could be used to increase the bioactivity of AM. The mycelium-fermented AM might be a valuable source of functional material and edible resource for industry.

Transfer of Isolated Nuclei from Agrocybe aegerita Mycelia into Pleurotus florida Protoplasts (사철느타리버섯 원형질체내(原形質體內) 버들볏짚버섯 핵(核)의 전이(轉移))

  • Yoo, Young-Bok;Shin, Pyung-Gyun;Kim, Han-Kyoung;Byun, Myung-Ok;You, Chang-Hyun;Cha, Dong-Yeul;Chang, Kwon-Yawl
    • The Korean Journal of Mycology
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    • v.17 no.3
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    • pp.114-118
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    • 1989
  • The transfer of isolated nuclei from Agrocybe aegerita mycelia of wild type into Pleurotus florida protoplasts of auxotroph was induced with polyethylene glycol. The type 1 of nuclear transfer products was spontaneous segregants of both parental morphology of colony. Hyphae of A. aegerita type had clamp connections while that of P. florida type lacked. Type 2 was main products of nuclear transfer which formed true clamp connections. Type 3 was clampless products. They all produced primordia and developed basidiocarps similar to Agrocybe aegerita. A comparison of nuclei transfer products was made using isozyme analyses of alcohol dehydrogenase, esterase, lactate dehydrogenase and peroxidase. Isozyme band patterns of some type 2 strains produced a new band. Band patterns from mycelial extracts of the other strains could be characterized by parental bands.

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Effect of Mycelia Extracts from Lentinus edodes Mushroom-Cultured Lonicera japonica Thunberg on Anticancer and Antiallergy Activities (인동초로 배양한 표고버섯 균사체 추출물의 항암 및 알레르기 억제효과 검증)

  • Bae, Man-Jong;Ye, Eun-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.4
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    • pp.424-430
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    • 2007
  • This study was conducted to investigate the effect of mycelia of Lentinus edoes mushroom-cultured Lonicera japonica Thunberg (LLJ) on proliferation of the cancer cell lines (Hep3B, MCF-7 and HeLa), sarcoma 180 (S-180) and antiallergy. In an anti-cancer test using Hep3B (hepatic cancer cell), MCF-7 (breast cancer cell) and HeLa (uterine cancer cell), LLJ extract showed higher antiproliferating effect than that of LJ (Lonicera japonica Thunberg) extract. In an anti-cancer testing using Hep3B cells, LLJ extract showed growth-inhibitory effect of $85.60{\pm}4.66%$ at 3mg/rnL. In an anti-cancer testing using MCF-7 cells, LLJ and LJ extracts showed high antiproliferating effect. LLJ showed the tumor suppressive effect in mice injected with S-180 cells. The growth-inhibitory rates against tumor cells were 61% for LLJ, 37% for LJ. LLJ inhibited histamine release from rat peritoneal mast cells activated by compound 48/80. These results suggest that Lentinus edodes mushroom-cultured herb has an antiproliferating effect against cancer cell lines (Hep3B, MCF-7 and HeLa) and S-180 tumor, and will be beneficial in the treatment of allergic reaction.

Comparison of anti-inflammatory, antioxidant and anti-allergic effects of Ganoderma species mycelial extracts (영지버섯 균주별 균사체 추출물의 항염, 항산화 및 항알러지 효능 비교 분석)

  • Park, Young-Jin;Nam, Jae-Young;Yoon, Dae-Eun;Kwon, O-Chul;Kim, Hong-Il;Yoo, Young-Bok;Kong, Won-Sik;Lee, Chang-Soo
    • Journal of Mushroom
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    • v.11 no.2
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    • pp.111-115
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    • 2013
  • This study wascarried out to compare the medicinal effects of various Ganoderma species mycelial extracts. Among 6 Ganoderma species mycelial extracts by using 100% MeOH, G. species ASI-7150 showed the highest antioxidant effect. In nitric oxide (NO) production and ${\beta}$-hexosaminidase release inhibition assay, the treatment of G. lucidum ATCC64251 (Taiwan) mycelia extracts most effectively inhibited NO production in LPS-stimulated RAW264.7 cells and ${\beta}$-hexosaminidase release. In addition, the treatment of all 6 Ganoderma species mycelial extracts were not affect on RAW264.7 cell viability. Although this preliminary research has thrown up many questions in need of further investigation, it will serve as a base for further studies of medicinal effects of various Ganoderma species.

Screening of mushroom having anti-angiogenic activity for development of new cancer metastasis Inhibitor (버섯으로부터 새로운 암전이 억제물질 개발을 위한 혈관신생 억제물질의 탐색)

  • Jeong, Seung-Chan;Kim, Jae-Ho;Park, Jeong-Sik;Lee, Jong-Soo
    • Journal of Mushroom
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    • v.1 no.1
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    • pp.44-47
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    • 2003
  • This study was carried out to obtain new cancer metastasis inhibitor from mushrooms. Extracts from 52 isolates belong to 7 species of mushrooms were prepared by water, ethanol and methanol extractions and its anti-angiogenic activity were investigated by choriollantoic membrane(CAM) assay. Water extracts of Fomitella fraxinea ASI 17003 and ASI 17009 fruiting bodies, ethanol extract of Pholiota sp. ASI 24008 and Grifola frondosa ASI 9017 fruiting bodies and methanol extract of Inonotus obliquus ASI 74012 mycelia had the potential anti-angiogenic activity of 62.5%~68.8%. Finally, Pholiota sp. ASI 24008 was selected as a producer of cancer metastasis inhibitor on the basis of their solid yield and anti-angiogenic activity, etc.

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Optimum Extraction Conditions and Anticancer Effect of Functional Polysaccharide from Mycelia of Grifola frondosa (잎새버섯(Grifola frondosa) 균사체의 기능성 다당류 최적 추출방법 및 항암효과)

  • Park, Chan-Ho;Lee, Gyeong-Min;Nam, Eun-Jeong;Yu, Yeon-Hee;Kim, Yong-Hyun;Kwon, Hyun-Jung;Yoon, Ok-Hyun;Han, Man-Deuk
    • The Korean Journal of Food And Nutrition
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    • v.25 no.1
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    • pp.181-187
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    • 2012
  • Grifola frondosa has been used as an herbal medicine for the treatment of cancer, diabetes mellitus and high blood pressure. In this study, functional polysaccharide was obtained from Grifola frondosa using four different extraction methods: hot water(HwFP), homogenize(HgFP), acid(AcFP), and alkali(AlFP) extraction methods. The effects of these extracts on KB and HepG2 cell lines were then examined for any anti-cancer activity. Alkaline extraction produced a yield of 0.175% and the total sugar content of the extract was 54.97%. We were able to confirm that the polysaccharide extracts from the mushroom produce an anti-cancer effect. The cytotoxicity of AlFP and AcFP against HepG2 cells were 22.86% and 28.88%, respectively, and the cytotoxicity of AlFP against the KB cell lines was 47.76% at a concentration of 1,000 ${\mu}g/m{\ell}$. Therefore, these results suggest that the optimum method for extracting functional polysaccharides from G. frondosa is the alkali extraction method.

Effect of Ponciri Fructus Extracts Fermented with Ganoderma lucidum on the Collagen Synthesis and Expression of Matrix Metalloproteinase-1 (콜라겐 합성과 MMP-1 발현에 대한 생물전환 지실 추출물의 효과)

  • Lee, Gye Won;Park, Sung Min;Yoo, Yung Choon;Cho, Young Ho
    • KSBB Journal
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    • v.28 no.2
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    • pp.106-114
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    • 2013
  • Ponciri fructus, the unripe fruits of Poncirus trifoliata, are widely used in oriental traditional medicine as a remedy for inflammation, gastritis, emesis, digestive ulcers, allergy, and dysentery. To study the anti-wrinkle effects of Ponciri fructus extract (PFE) containing flavanone glycosides, PFE was fermented with Ganoderma lucidum mycelia and its biological activities were investigated. In Ponciri fructus extracts fermented with G. lucidum (G-PFE), polyphenol content was $1,021.00{\pm}0.50{\mu}g/mL$ and flavonoid content was $589.41{\pm}0.21{\mu}g/mL$. G-PFE was found to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and superoxide anion radical by a dose dependent manner, respectively. G-PFE showed higher antioxidant activity than that of PFE. In addition, the photoprotective properties of G-PFE was tested in human dermal fibroblasts (HDF) exposed to UVA radiation. G-PFE inhibited the activity of matrix metalloproteinase-1 (MMP-1) and showed a dose dependent decrease in the expression level of MMP-1. G-PFE also increased collagen biosynthesis in HDF. These results demonstrate that G-PFE could be useful as a potential cosmetic ingredient for anti-wrinkle.

Anti-Pigmentation Effects of Eight Phellinus linteus-Fermented Traditional Crude Herbal Extracts on Brown Guinea Pigs of Ultraviolet B-Induced Hyperpigmentation

  • Ahn, Hee-Young;Choo, Young-Moo;Cho, Young-Su
    • Journal of Microbiology and Biotechnology
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    • v.28 no.3
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    • pp.375-380
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    • 2018
  • We have previously found that mycelia culture broth of eight kinds of traditional herbal extracts fermented with Phellinus linteus (previously named as 8-HsPLCB) not only inhibited melanin and tyrosinase activity, but also reduced the contents of melanogenesis-related proteins, including tyrosinase and microphthalmia-associated transcription factor, in 3-isobutyl-1-methylxanthine-stimulated B16F0 melanoma cells. For a further study, the effect of 8-HsPLCB against skin pigmentation in brown guinea pigs with ultraviolet B (UVB)-induced hyperpigmentation was investigated. 8-HsPLCB (3%) and arbutin (2%) as positive controls were applied topically twice daily for 4 weeks to the hyperpigmented areas. 8-HsPLCB showed skin-lightening effect as effective as arbutin, one of the most widely used in whitening cosmetics. Melanin index values as the degree of pigmentation showed a significant reduction week by week post 8-HsPLCB treatment and then substantially reduced by 4 weeks. The degree of depigmentation after 4 weeks of topical application with 8-HsPLCB was 32.2% as compared with before treatment (0 week). Moreover, using Fontana-Masson staining and hematoxylin-eosin staining, 8-HsPLCB reduced melanin pigmentation in the basal layer of the epidermis and epidermal thickness changes exposed to the UV-B irradiation as compared with non-treatment and vehicle treatment. The intensity of the skin-lightening effect of 8-HsPLCB was similar to arbutin. These results suggest that the skin-lightening effect of 8-HsPLCB might be resulted from inhibition of melanin synthesis by tyrosinase in melanocytes. To conclude, 8-HsPLCB treatment showed reduction of the melanin pigment and histological changes induced by UV irradiation in brown guinea pigs.

Studies on Development of New Basidiomycetes by Protoplast Fusion and Nuclear Transfer I - The Antitumor Components of the Protoplast fusants - (원형질체 융합 및 핵전이에 의한 새로운 담자균류의 개발에 관한 연구(I) - 융합균사체의 항암성분 -)

  • Moon, Chul;Yoon, Jong-Myung;Kim, Chae-Kyun;Kim, Ha-Won;Choi, Eung-Chil;Kim, Byong-Kak
    • Korean Journal of Pharmacognosy
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    • v.27 no.3
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    • pp.224-230
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    • 1996
  • To find pharmacologically active hybrids among the inter-order protoplast fusants of Lentinula edodes and Ginoderma lucidam the antitumor test was performed and the fusant P22 was selected among them. The hot water and alkaline extracts from the cultured mycelia of P22 were purified and separated into four fractions by DEAE-cellulose anion exchange chromatography. When a dose of 20 mg/kg/day of each fractions was injected into ICR mice by i.p., the tumor inhibition ratio of Fr. IV against solid sarcoma 180 was the higher than any other fraction. Fr. IV was a protein-bound polysaccharide which was composed of 69. 12% polysacchafide and 9.76% protein and the molecular weight of Fr. IV was $6.7{\times}10^4$ dalton.

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