• Animal Bioscience
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• v.34 no.2
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• pp.285-294
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• 2021

Objective: This study was conducted to investigate the supplementary effect of a phytogenic blend (SPA: a mixture containing fermented Schisandra chinensis pomace, fermented Pinus densiflora needle extract, and Allium tuberosum powder in the ratio of 2:2:1) on egg production, egg quality, blood constituents, and visceral organs in laying hens. Methods: A total of 135 Hy-line brown laying hens (48-wk-old) were randomly allocated to three dietary treatments with 5 replicates of 9 hens. The control group (CON) was fed a basal diet (no exogenous SPA addition) and the experimental groups were fed the basal diet containing SPA at the level of 0.1% and 0.3% for 6 weeks. Results: The feed intake was significantly improved in SPA supplemented groups as compared with the control (p<0.05). However, egg production, daily egg mass, and feed conversion ratio were not different among the dietary treatments (p>0.05). For egg quality traits, only Haugh unit (HU) was significantly improved in SPA (0.3%) (p<0.05) as compared with other groups. However, HU was not affected during 4-wk of storage at 18℃ among the dietary treatments (p>0.05). Furthermore, SPA supplementation did not affect the blood biochemical constituents except for the phosphate content, which was significantly higher in SPA groups than the CON group (p<0.05). There were no significant differences in visceral organ characteristics and immune indicators (immunoglobulin A [IgA], IgG, and IgM) in SPA or CON groups. Conclusion: This study suggested that the supplementation of SPA may have beneficial effects on feed intake and egg quality in laying hens.

• Journal of Ecology and Environment
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• v.29 no.1
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• pp.23-27
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• 2006

This study was conducted to evaluate forest carbon cycling and soil $CO_2$ efflux rates in a 42-year-old pine (Pinus densiflora) stand located in Hamyang-gun, Korea. Aboveground and soil organic carbon storage, litterfall, litter decomposition, and soil $CO_2$ efflux rates were measured for one year. Estimated aboveground biomass carbon storage and increment in this stand were $3,250gC/m^2\;and\;156gC\;m^{-2}yr^{-1}$, respectively. Soil organic carbon storage at the depth of 30 cm was $10,260gC/m^2$ Mean organic carbon inputs by needle and total litterfall were $176gC\;m^{-2}yr^{-1}\;and\;235gC\;m^{-2}yr^{-1}$, respectively. Litter decomposition rates were faster in nne roots less than 2 mm diameter size ($<220\;g\;kg^{-1}yr^{-1}$) than in needle litter ($<120\;g\;kg^{-1}yr^{-1}$). Annual mean and total soil respiration rates were $0.37g\;CO_2m^{-2}h^{-1}$ and $2,732g\;CO_2m^{-2}yr^{-1}$ during the study period. A strong positive relationship existed between soil $CO_2$ efflux and soil temperature (r=0.8149), while soil $CO_2$ efflux responded negatively to soil pH (r=-0.3582).

• Journal of Korean Society of Forest Science
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• v.24 no.1
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• pp.53-61
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• 1974

Pine forests existing in Hong-do island were examined as regarding such characteristics, tree form, needle length, branch diameter, bark colors, ratio of summer wood to springwood, location and number of resin canal and other measurements. Hong-do island, located the furthest west part of Korea, suports only Pinus densiflora but very few Pinus thunbergii, in fact the present authors could not find any black pine individuals at all through investigation. Checking 14 individual trees of red pine, more than age of 20, of the genetic contamination by black pine, in a certain degree, exist. Here, a very dense foliage type and a very sparse foliage type tree of red pine growing nearby was examined and the unique fact was that each of those type has only one (average number 1.3) accessory resin canal, while other trees have three as minimum or more. The present authors thought this island had not been covered by any Pinus thunbergii but by Pinus densiflora. It was thought that Pinus thunbergii stands grown along the sea-coast of Korean Peninsula are pollen donors to this island.

• Korean Journal of Environment and Ecology
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• v.6 no.1
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• pp.1-8
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• 1992

Artificial acid rain (pH 3.0, 4.0 and 5.0) was treated on the seeded pots of 4 species to examine its effects on germination, leaf injury and seedling growth. Artificial acid rain was prepared by diluting sulfuric acid with ground water and ground water (pH 6.5) was used as control. Artificial acid rain was sprayed to seeded pots three times per week. About 5mm of artificial acid rain was treated each time from early April to early October, 1991. Germination, seedling establishment, leaf injury, soil acidity and seedling growth were measured and compared among the treatments. The results were summarized as follows; 1. Germination and seedling establishment rate of Pinus thunbergiana was highest on the pH 4.0 pot whereas that of Picea abies was highest on the pH 3.0 pot. Those of Pinus densiflora and Abies holophylla were not significantly differed among the treatments. 2. The differences in seedling height of all species were not significantly differed among the treatments. 3. Seedling dry weight of Pinus densiflora differed significantly among the treatments. but that of Pinus thunbergiana did not differ. 4. With decrease of pH value of artificial acid rain. the number of injured needle and injured individual tended to be increased. 5. The differences of soil acidity were significant among the treatments for all species.

• Journal of Life Science
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• v.8 no.1
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• pp.91-96
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• 1998

Pine(Pinus densiflora Sieb et Zucc.) is one of rhe popular plant drugs which has been used as a medicine in Asia. To investigate the effect of pine needle extract (PNE) on oxygen radicals and their scavenger enzymes in brain membranes of Sprague- Dawley (SD), make SD rats were fed basic diets(control group), and experimental diets (PNE group) with 0.5 and 1.0% of PNE 6 weeks. Mitochondrial hydroxyl radical levels in brain of 0.5%-PNE and 1.0%-PNE groups were significantly inhibited to 30% and 25%, respectively, and microsomal hydrogen peroxide levels in brain of 0.5%-PNE and 1.0%-PNE groups were significantly inhibited to 15% compared with control group. Cytosolic superoxide rdical levels in 1.0%-PNE group were significantly inhibited to 20% compared with control group. Lipid peroxide(LPO) levels in brain mitochondria of 0.5%-PNE and 1.0%-PNE groups were significantly lower(25% and 35%) than that in control group. Mn-superoxide disumtase (SOD) activities in brain of 0.5%-PNE and 1.0%-PNE groups were significantly higher(18% and 12%) than those in control groups, but Cu,Zn-SOD activities in brain of 0.5%-PNE were significantly activated to 15% compared with control group. Glutathione peroxidase(GSHPx) activities in brain of 1.5%-PNE and 1.0% PNE groups were significantly higher(14% and 12%) than those in control group. These results suggest that more beneficial effects such as inhibition of oxygen radicals and lipid peroxide(LPO). and oncreases of scavenger enzymes in brain membranes of SD rats may be effectively modulated by administration of pine needle extract (PNE)

• The Korean Journal of Ecology
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• v.15 no.2
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• pp.147-155
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• 1992

Litterfall, decomposition, and dynamics of n and p in decomposing litter were investigated for 2 years in red pine ( pinus densiflora) and chinese thuja( thuja orientalis)stands in the limestone area. Average litterfall in red pine and chinese thuja stands were 4, 535kgDM ha-1 yr-1 and 5, 010 kgDM ha-1 yr-1, respectively. Seasonal litterfall in red pine and chinese thuja stands showed peaks in November. concentrations of N and P in the needle litter were lowest in the winter when the greast litterfall occurred, and highest in the summer when the least litterfall occurred. However, those in chinese thuja scale leaf litter showed litter seasonal variation.amount of N and P returned to the forest floor through litterfall were 29.02kgN ha-1 yr-1 , 2.81 kg P ha-1 yr-1 for red pine stand, and 31.06 kg N ha-1 yr-1 , 2.86kgP ha-1 yr-1 for chinese thuja stand, respectively. After 21 mounts elapsed, needle and chinese thuja scale leaf litterbags lost 34.8% and 32.5% of the initial weight, respectively. N concentrations in the docomposing needle and chines thuja scale leaf litter decreased by 19% and 30%, respectively, after 1 month elapsed, and then gradually increased to exceed the initial concentration after 9 months elapsed and then gradually increased to exceed the initial concentration after 9 months elapsed in both of them, P in needle and chines thuja scale leaf litter decreased by 54% and 57% of the initial concentration, respectively, after 1 month elapsed. unlike N, P concentration in the decomposing litter did not exceed that of initial ones. Neyt immobilization period of N and P in decomposing litter did not occur over the study period.

• Journal of Korean Society of Forest Science
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• v.90 no.5
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• pp.632-642
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• 2001

Fauna, especially Carabidae (Coleoptera), on the floor of Pinus densiflora forests was investigated at five plantations and was compared with that in a mixed natural forest of pine and deciduous trees. There was little difference in the ground beetle fauna among the five P. densiflora plantations, and the bio-diversity of soil layer fauna was poor in all the pine plantations. Individual numbers of the beetles belonging to the genus Synuchus with increasing the infestation rate of the pine needle gall-midge (Thecodiplosis japonensis). These beetles seem to be important predators of the gall-midge. Comparing to the mixed forest, the number of higher taxa and the numbers of genera and species of Carabidae were all small in the pine forests. And especially, most flightless species were found only in the mixed forest. Thus, it is concluded that many species of Carabidae which had been lost from the lower mountainous areas of Korea have not recovered yet in the pine forests.

• Korean Journal of Plant Resources
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• v.20 no.4
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• pp.336-341
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• 2007

This study was carried out to investigate geographic variation in needle characteristics of Pinus densiflora, one of the most economically important tree species in Korea. Needles were collected from 35 natural populations and their morphological and anatomical characteristics were studied. ANOVA showed statistically significant differences among populations as well as among individuals within populations in needle length, needle width, number of stomata row, density of stomata in $0.25mm^{2}$, number of serrations in 0.5mm, and number of resin ducts. In all these traits, variance components among individuals within populations were larger than those among populations. The density of stomata in $0.25mm^{2}$ increased as the latitudes and elevations of the populations increased, while the number of serrations in 0.5mm decreased as the decrease of latitudes and elevations. The number of resin ducts was higher in inland populations than in coastal populations. The resin duct index appeared to be higher in western populations than in eastern populations. Cluster analysis based on morphological characteristics divided populations into three groups. The third group was composed of populations selected from the Taebaek mountains. The number of serrations in 0.5mm of this group was lower than that of other two groups, while the density of stomata in $0.25mm^{2}$ was higher.

• Journal of Korean Society of Forest Science
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• v.62 no.1
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• pp.87-95
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• 1983

The effects of the pine needle gall midge, Thecodiplosis japonensis, on the growth of the Korean red pine, Pinus densiflora, were examined for the natural forest stands at nine locations: Seocheon-gun, Boryong-gun, and Hongseong-gun in Chooncheongnam-do, where consequence of the infestation had been different. To estimate the economic threshold level of the pine needle gall midge, the growth impacts due to the insect were examined with terminal shoot growth, and cambial growth by analyzing the growth horizontally and vertically. The results obtained are as follows: 1) In general, the growth impacts of the red pine trees were significant for the five consecutive years since damage had occurred, and its maximum retardation was shown in the second and the third years. 2) Simultaneous retardation occurred in both terminal and cambial growth of the tree, probably due to cumulative characteristics of the feeding behaviour. 3) Strong correlation was found between terminal and cambial growth of Korean red pine and this was true for the trees infested by pine needle gall midge. Thus retardation in cambial growth due to the insect may be estimated with that in terminal growth. 4) Infestation was greater on slope facing north than south even though the history of the infestation had been similar. 5) The stem volume of 13-year-old, infested Korean red pine was reduced by more than 75-85%, compared to same aged, uninfected pine in yield table. Thus, much longer period is required to recover the normal growth.

• Journal of Korean Society of Forest Science
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• v.50 no.1
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• pp.49-55
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• 1980

Monoterpenes, phenolic substances, growth substances and total nitrogen from normal needles and infested needles attacked by Thecodiplosis japonensis were compared for Pinus densiflora, Pinus thunbergii and Pinus rigida. The results obtained in this study were as follows: 1. Major monoterpene components in P. densiflora needles were ${\beta}$-phellandrene, ${\alpha}$-pinene and those in P. rigida needles were ${\beta}$-pinene and ${\alpha}$-pinene. In P. rigida resistant to this insect, infested needles showed higher ${\alpha}$-pinene, ${\beta}$-pinene and myrcene but lower camphene, limonene and ${\beta}$-phellandrene than normal needles. 2. Orcinol, catechol ferulic acid, salicylic acid and five unknowns were detected in P. rigida needles whereas orcinol, catechol, ferulic acid and four unknowns in P. densiflora needles. 3. Tryptophan, a precursor of IAA, was detected in larvae and also in gall tissues of both P. densiflora and P. thunbergii needles. This fact shows that growth substances may involve in gall formation by Thecodiplosis japonensis. 4. Total nitrogen contents per unit needle weight were neither significantly different between P. densiflora and P. rigida, nor between normal and infested needles.