• Journal of Korean Society of Forest Science
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• v.65 no.1
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• pp.1-11
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• 1984

Susceptible trees of Pinus densiflora and resistant trees of Pinus rigida following pine gall midge (Tnecodiplosis japonensis Uchida et Inouye) attack were seasonally compared to examine the variation in needle growth and photosynthetic ability, respiration rate, chlorophyll contents, carotenoid and anthocyanin contents. Also, carotenoid and anthocyanin contents of larvae both from soil and from galled tissue were compared during March and September, respectively. The plantation damaged severely by this insect consisted mostly of 10-to 15-year old P. rigida and P. densiflora. The results obtained in this study were as follows: 1) The length of the infested needles of P. densiflora decreased by 48.1 percent compared with the normal needles, while that of P. rigida did 37.4 percent. 2) All of P. densiflora and P. rigida showed higher photosynthetic ability in normal needles than in infested needles. The maximum photosynthetic ability of P. densiflora was shown in mid-August, while that of P. rigida in mid-October. In contrast to that, respiration rate of infested needles was higher than that of normal needles in both species. The respiration rate of P. rigida was higher than that of P. densiflora. 3) P. rigida had higher total chlorophyll contents than P. densiflora. The total carotenoid contents tents in infested needles were higher than those in normal needles of both species. 4) Total carotenoid contents were generally higher in P. rigida than in P. densiflora during the growing season. The total carotenoid content (0.094mg/g) in larvae from soil was similar to that (0.092mg/g) in larvae from galled tissues. 5) Infested needles of both species showed higher anthocyanin contents than normal needles. Higher anthocyanin contents in galled needles were due primarily to its active formation stimulated by larval attack. Thus, reddish-brown coloration occurred only in galled needles of P. densiflora.

• Preventive Nutrition and Food Science
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• v.8 no.1
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• pp.7-12
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• 2003

This study examined the effect of pine needle distillate (Pinus densiflora Sieb. et Zucc) on the immune system and hematological parameters. C57BL/6 male mice weighing 20 ~21 g were divided into 3 groups and intraperitonially injected with either 200 $\mu$L of saline (control), 50% diluted (P50) or 100% pine needle distillate (P100) once a day for 24 days. At the end of the experiment, the mice were anesthetized by ether and peripheral blood was collected from the femoral artery and the spleen was excised. Spleen weight decreased significantly (p<0.001) in the pine needle groups compared to the control group. The blood was used for a complete blood count and flow cytometrical analysis after immunofluorescence staining. The pine needle distillate dose-dependently decreased the CD4$^{＋}$/CD8 sup ＋/ ratio (p <0.05), and showed a tendency to increase the mean FSC (forward scatter) values of the CD8$^{＋}$T cells, while decrease the values of the CD4$^{＋}$T cells. There were no significant differences in WBC, RBC and platelet counts among the three groups, but hemoglobin and hemoglobin-related parameters and platelet volume increased and red blood cell volumes decreased with the administration of the pine needle distillate. These results suggest that the pine needle distillate may have immunosuppressive effects.

• Journal of Korean Society of Forest Science
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• v.82 no.3
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• pp.271-282
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• 1993

This study was conducted to investigate the absorbing and/or filtering capacity of woody landscape plants growing in urban and industrial regions of Seoul and Taejon. Korea. Total sulfur content and heavy metal content were analyzed in tree foliage of Pinus densiflora, Pinus koraiensis, Ginkgo biloba. Liriodendron tulipifera, Platanus occidentalis. The total sulfur content in the leaves of woody landscape plants in Seoul and Taejon were remarkably higher than those of the tree leaves in Kwangreung. The total sulfur content of deciduous trees had 2.6 times more than that of coniferous trees. the former had better absorbing capacity of $SO_2$ gas. Especially Ginkgo biloba and Platanus occidentalis were recommanded as absorption trees of $SO_2$ because sulfur content levels of them were the highest among all species and increased until tall coloration. While coniferous trees showed no changes in total sulfur content between four seasons. deciduous trees increased in sulfur during the growing season. Pb content of all species in polluted area were higher than those in unpolluted area. In polluted area, while coniferous trees had higher absorption and accumulation of Pb, deciduous trees had higher absorption of Zn. The content of Pb, Zn and Cu in needle of Pinus koraiensis were more than those of Pinus densiflora, In Pinus koraiensis and Pinus densiflora, heavy metal content in the 2nd year needle were higher than thous in the current year needle. In Pinus densiflora, Pinus koraiensis Liriodendron tulipifera and Platanus occidentalis there was a positive correlation between total sulfur and heavy metals [(Pb+Zn+Cu)/3] content.

• Journal of Korean Society of Forest Science
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• v.7 no.1
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• pp.19-27
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• 1968

As a preliminary investigation to obtain useful auto- and allopolyploids a ten year old tetraploid of Pinus densiflora induced from the colchicine treated seed was observed on the cytological, morphological and physioa logical characters in the present study and the results can be summarized as follows. 1. The number of chromosome at the base of needle was 48 so that it was recognized as a tetraploid. 2. The needles were thicker and the number of them on an individual was less than the 2n plant. The needle combined to a single was appeared 2%. 3. No difference was found in the number of stomata rows on the central part of needle between the tetraploid and 2n plant, however, guard cells of tetraploid increased 36% in the long diameter compared with 2n plant. 4. Microsporangiate storbile developed normally showing the same size with 2n plant while the size of pollen grain increased about 20% larger than 2n plant. 5. Germination percentage in vitro increased until 17 hours but decreased after 26 hours compared with 2n plant. The longest length of pollen tube during the germination period appeared in the tetraploid pollen.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.4
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• pp.423-429
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• 2009

This study was conducted to investigate the effects of pine (Pinus densiflora) needle extracts on hepatic antioxidative system in rats fed high-cholesterol diet. Eight-week old Sprague Dawley rats were divided into four groups; normal diet group (CON), 0.5% cholesterol diet group (0.5% CHOL), 0.5% cholesterol diet and pine needle extracts prepared by using hot water group (HOT water), and 0.5% cholesterol diet and pine needle extracts prepared by using sub-supercritical $CO_2$ group (Sub-supercritical). The serum ALP, AST, and ALT activities were increased by 0.5% cholesterol diet supplementation, but treatment with pine needle extracts showed decrease compared with 0.5% CHOL group. The SOD activity in liver at 0.5% cholesterol diet group was more decreased than control group while Sub-supercritical group had significantly higher SOD activity than 0.5% CHOL group. Moreover, groups with pine needle extracts supplementation had higher level of GPx in liver than 0.5% cholesterol diet group. These results suggest that pine needle extracts increased antioxidative activities.

• Journal of Korean Society of Forest Science
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• v.85 no.3
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• pp.372-380
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• 1996

This study was carried out to study the resistant substances to pine needle gall midge and seasonal variation of phenolic compounds in pine needles among susceptible and resistant species to pine needle gall midge. Free and water soluble phenolic compounds contained in pine needles showed seasonal variations among pine species. But, catechol, vanillic acid and syringic acid including phenolic compounds showed no variation between pine species, even the amount of those component varied by the seasons. Salicylic acid in pine needles showed 140ppm in Pinus virginiana, 35ppm in Pinus thunbergii and 72ppm in hybrid pine, Pinus thunbergii${\times}$P. virginiana, but traces in Pinus densiflora on May and June, hatching season in larvae of pine needle gall midge. There was high level of salicylic acid in resistant species to pine needle gall midge but traces in susceptible species. As our previous researches, the necrosis rate of larvae of pine needle gall midge showed high level on the solution of phenolic compounds extracted from needles of P. virginiana and salicylic acid, and there was no gall formation in P. virginiana, so that salicylic acid in pine needles seems to have relation with those results. Therefore, it is estimated that salicylic acid is resistant substance to pine needle gall midge.

• Journal of Environmental Science International
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• v.17 no.10
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• pp.1139-1146
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• 2008

To investigate the functional properties and the antioxidant effect of pine needle(Pinus densiflora), the pine needle extract was obtained with methanol and its functionality was measured by spectrophotometric method, and the antioxidant experiment on soybean oil was carried out by the active oxygen method. The extraction yield of pine needle with 99% methanol was about 19%, the total flavonoid content of the pine needle-methanol extract was 11.32 mg/g on dry basis and the superoxide dismutase-like activity was 94.3%. The nitrite scavenging ability of the extract was pH dependent with the values of 77.44% at pH 1.2, 48.45% at pH 3.0 and 11.04% at pH 6.0, respectively. The peroxide value was 92.6 meq/kg at 5% dosage, 138.4 meq/kg at 2% dosage of the extract on 8 oxidation days. The period of the peroxide value to be 100 mg/kg was 4.9, 6.3 and 8.5 days at control, 2% and 5% dosage of extract, respectively. And the relative antioxidant effectiveness of the extract was 27.9% and 72.3% increase at 2% and 5% dosage, respectively, compared to control. The thiobarbituric acid value showed few differences within 4 oxidation days, but with the dosage of the extract it fairly decreased with considerable antioxidant effect to control above 4 days.

• Applied Chemistry for Engineering
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• v.21 no.6
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• pp.664-669
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• 2010

The forest area of domestic is 6370304 ha, which covers 70% of the whole country, and especially Gangwon-do is remarkably larger than other Province. A thick forest of the country has the most basic component among other natural environments as well as it has invaluable worth to human being such as scientific research and educational value. However due to the breakout of forest fire since 1990s, the loss of trees, destruction of natural environment and ecology, economic damage have been occurring and its scale also has become larger. The causes of becoming larger in scale are resulted from forest components which mainly consist of needle leaf trees, wide leaf trees, fallen leaves, herbaceous plants so that it has been a direct cause for forest fire. However, few research on combustion and pyrolysis characteristics has been done in domestic and abroad. The study on the combustion and pyrolysis for Pinus densiflora which are typical needle leaf trees has been tried using TGA. Pinus desiflora started to being ignited at around $162^{\circ}C$ and pyrolysis was done at around $197^{\circ}C$. Differential method was applied to calculate activation energy and frequency factor according to the variation of conversion. Activation energy in pyrolysis was increased from 79 kJ/mol to 487 kJ/mol with increasing conversion and average activation energy was 195 kJ/mol. The activation energy in combustion was decreased from 148 kJ/mol to 133 kJ/mol.

• Journal of Forest and Environmental Science
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• v.17 no.1
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• pp.37-46
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• 2001

The objective of this work is to examine anatomical and physical characteristics of wood damaged by pine needle gall midge(PNGM, Thecodiplosis japonensis Uchida et Inouye) in Pinus densiflora. In anatomical characteristics, it was noted that the number of axial and radial resin canals in the damaged wood was higher than that in sound wood. In damaged wood, green moisture content of sapwood and swelling and shrinkage was higher than sound wood. Both longitudinal compressive strength and bending strength of the damaged wood were lower than those of sound wood. On the other hand, shearing strength of damaged wood was similar to that of sound wood.

• Journal of Life Science
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• v.9 no.4
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• pp.473-480
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• 1999

This study was designed to investigate the effects of pine (Pinus densiflora Sieb et Zucc) needle extract (PNE) on membrane fluidity and oxidative stress in liver membranes of Sprague-Dawley (SD) rats as a study on investigation of anti-aging effect and determination of chemical structures of PNE through the animal experiments. Male SD rats were fed basic diets (control group) and experimental diets (0.5% and 1.0%-PNE group) for 6 weeks. Administrations of 0.5% and 1.0%-PNE resulted in a marked decreases (15∼25% and 23∼26%, respectively) in cholesterol accumulations of liver mitochondria and microsomes compared with control group. Membrane fluidities were significantly increased (15∼25%) in liver microsomes of 0.5% and 1.0%-PNE groups compared with control group. Formations of basal and induced oxygen radicals (BOR and IOR) in liver mitochondria were significantly inhibited (11∼12% and 10∼15%, respectively) by administrations of 0.5% and 1.0%-PNE compared with control group. Lipid peroxide (LPO) levels were remarkbly decreased about 20% in liver mitochondria and microsomes of 0.5% and 1.0%-PNE groups compared with control group. Oxidized protein levels calculated with carbonyl group were significantly decreased about 15% in liver mitochondria of 1.0%-PNE group compared with control group. These results suggest that PNE may play a effective role in a attenuating a oxidative stress and increasing a membrane fluidity.