• 한국수산과학회지
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• 제30권2호
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• pp.244-251
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• 1997

생리활성을 지닌 신경펩타이드의 구조와 활성간의 관계를 규명하기 위하여 고상법으로 합성한 세 종류의 neuropeptide $\gamma$(mammalian-, trout- 그리고 goldfish-neu-ropeptide $\gamma$)를 사용하여 연구하였다. Circular dichroism spectra에 의하면 mammalian-, trout-와 goldfish-neurope-ptide $\gamma$는 완충액 조건하에서 모두 random한 구조를 나타내었다. 중성 및 산성 지질 존재 하에서, mammalian과 trout-neuropeptide $\gamma$는 여전히 random한 구조를 취했다. 그러나, goldfish-neuropeptide $\gamma$는 중성 및 산성지질하에서 부분적으로 $\alpha-helix$ 구조를 나타내었다. 장관 수축활성 에 있어서는 carp 장관, guinea-pig 회장 그리고 rat 십이지장에 대하여 비교하였다. Carp에 대해서는 goldfish-neuropeptide $\gamma\;\simeq$ trout-neuropeptide $\gamma\;>$ mammalian- neuropeptide $\gamma$ 순으로 활성이 나타났다. 그러나, guinea-pig 회장과 rat 십이지장에 대해서 mammalian-neuropeptide $\gamma$는 어류 유래성 neuropeptide g들 보다 높은 수축활성을 나타내었다. 이러한 결과들은 neuropeptide $\gamma$들이 종-특이적인 활성을 나타낸다는 것을 제시한다.

• Restorative Dentistry and Endodontics
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• 제21권1호
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• pp.366-374
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• 1996

The purpose of this study was to investigate whether D-myo-inositol-l,2,6-trisphosphate (PP56) can effectively antagonize vasoconstriction caused by neuropeptide Y in the dental pulp, and to understand involvement of neuropeptide Y in the regulation of microcirculation in the dental pulp with the aim of elucidating neurogenic inflammation. Experiments were performed on 7 cats anesthetised with sodium pentobarbital, and neuropeptide Y and a neuropeptide Y antagonist PP56 were injected close intra-arterially into the dental pulp. The probe of laser Doppler flowmeter was placed on the buccal surface of ipsilateral canine teeth to the drug administration and pulpal blood flow was measured. Intra-arterial injection of neuropeptide Y (1.3-$2.0\;{\mu}g$/kg) resulted in pulpal blood flow decrease of $37.73{\pm}5.73%$(mean${\pm}$SEM) (n=9). Intra-arterial injection of PP56(0.3 mg/kg) alone changed pulpal blood flow little by 1.03 % reduction. The effect of neuropeptide Y in the presence of PP56 resulted in significantly less decreases in pulpal blood flow ranging from $27.17{\pm}5.37$ to $16.63{\pm}3.48%$ from control as compared with neuropeptide Y alone(n = 13). In effect, PP56 attenuated pulpal blood flow caused by neuropeptide Y. Results of the present study have provided evidences that a non-peptide PP56 is capable of antagonizing vasoconstriction caused by neuropeptide Y in the feline dental pulp. In addition, they show functional evidences that neuropeptide Y plays an active role in modulating the microcirculation of the dental pulp.

• 한국수산과학회지
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• 제32권2호
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• pp.232-237
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• 1999

Tachykinin peptide의 구조와 생리활성간의 상관관계를 조사하기 위해 고상법으로 합성한 어류 유래의 neuropeptide $\gamma$(Mammalian-, Bowfin- 그리고 Shark-neuropeptide $\gamma$)를 사용하였다. 이들의 이차 구조를 알아보기 위해 circular dichroism (CD)을 이용하였다. CD 연구 결과에 따르면, mammalian-neuropeptide $\gamma$, bowfin-neuropeptide $\gamma$와 shark-neuropeptide $\gamma$는 완충액과 인공지질막 조건하에서 random구조를 나타내었다. 또한 이들 neuropeptide $\gamma$의 장관에 대한 수축 활성을 조사하기 위해 guinea-pig의 회장, rat의 십이지장과 carp intestine을 사용하였다. Carp intestine에 서 bowfin-neuropeptide $\gamma$>shark- neuropeptide $\gamma$>mammalian-neuropeptide $\gamma$순으로 활성 이 나타났다. 그러나, guinea-pig 회장과 rat 십이지장에 대해서 mammalian-NP$\gamma$의 활성이 어류 유래의 neuropeptide $\gamma$들보다 높게 나타났다. 이러한 결과들은 neuropeptide $\gamma$가 종-특이적인 활성을 나타냄을 제시한다.

• Bulletin of the Korean Chemical Society
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• 제35권1호
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• pp.158-164
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• 2014

Substance P and neuropeptide Y were discovered as early diagnostic biomarkers of acute myocardial infarction in Korean patients and confirmed using enzyme-linked immunosorbent assay (ELISA). We screened 12 peptides from the sera of Korean acute myocardial infarction (AMI) patients and detected 3 peptides (neuropeptide Y, substance P, and N-terminal pro-B-type natriuretic peptide) to be elevated from patients' sera by liquid chromatography mass/mass spectrometry. The elevated concentration of 3 peptides was confirmed by ELISA. The screening results revealed the substance P, neuropeptide Y, and pro-B-type natriuretic peptide (47-76) concentrations were higher in patients' sera than in healthy controls. The sensitivity and specificity of substance P for AMI diagnostic marker were 80% and 83%, respectively, and those of neuropeptide Y were 87% and 90%, respectively compared to healthy controls. These results suggest that substance P and neuropeptide Y could be used as early diagnostic biomarkers in patients with AMI.

• 통합자연과학논문집
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• 제5권2호
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• pp.65-71
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• 2012

Neuropeptide Y (NPY), a 36-amino acid polypeptide, is a member of the pancreatic polypeptide family, which consists of NPY, peptide YY (PYY) and pancreatic polypeptide (PP). The neuropeptide Y (NPY) receptors called Y receptors belongs to G-protein coupled that are involved in a variety of physiological functions such as appetite regulation, circadian rhythm and anxiety. Five receptor subtypes have been cloned in mammals (Y1, Y2, Y4, Y5, and Y6) of which four are functional. In this short review, information about the functional NYP receptors was analyzed. Sequence analyses were done between these receptors to identify the relationships between them. Phylogram was generated between these receptors to identify the close homologue between these receptors. Our sequence analyses found that Y1 and Y4 receptors are close than the other receptors. Further structure based analysis could be useful to identify subtype selective antagonists and dual antagonists targeting Y1 and Y4 receptors.

• Journal of Nutrition and Health
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• 제34권7호
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• pp.727-733
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• 2001

The present study was conducted to identify the mechanism about the regulation of appetite by examining the expression patterns of neuropeptide Y in the hypothalamus of mice either fasting mouse for 24 hours or with anorexia mutant mouse. In order to investigate the patterns of expression of neurpeptide Y, immunohistochemistry was employed for measurements at the tissue level, along with the molecular biological techniques of reverse transcription polymerase chain reaction(RT-PCR) and dot blotting. The results of this study are as follows. The level of expression of neruopeptide Y, a neuropeptide known to enhance appetite, was shown to be lowered in the arcuate nucleus(ARC), paraventricular nucleus(PVN), lateral hypothalamic area(LHA), and dorsomedial hypothalamic nucleus(DMN) in both the fasting and anorexia mutant groups when measured via immunohistochemistry, a tissue-level method. RT-PCR and dot blotting, the molecular biological methods employed in this study, revealed that the level of neuropeptide Y mRNA in the entire hypothalamus was similar in the control and fasting groups and lower in the anorexia mutant group. The results of the present study showed that while the levels of expression of the neuropeptide Y in the various hypothalamic regions studied did not exhibit regular increases or decreases when measured immunohistochemically. But the entire hypothalamus via molecular biological methods showed that the changes in these levels were more definite in the anorexia mutant group than in the fasting group.

• 대한수의학회지
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• 제36권4호
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• pp.821-828
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• 1996

To elucidate the neuromodulation of neuropeptide Y and $\alpha,\;\beta$-methylene ATP on the neurogenic contraction of electrical perivascular nerve stimulation and the contractile response of noradrenaline from polygraph in the isolated renal artery of rabbit. 1. The neurogenic contraction induced by perivascular nerve stimulation was the voltage-dependent manner(10-100V) in the isolated renal artery of rabbit. 2. Neuropeptide Y(0.1uM) and $\alpha,\;\beta$-methylene ATP(1uM) increased the contractile responses of noradrenaline in the isolated renal artery of rabbit. 3. Neuropeptide Y(0.1uM) and $\alpha,\;\beta$-methylene ATP(1uM) increased the neurogenic contraction of electrical perivascular nerve stimulation in the isolated renal artery of rabbit. These results suggest that neuropeptide Y and $\alpha,\;\beta$-methylene ATP neuromodulated on the neurogenic contraction of electrical perivascular nerve stimulation on the isolated renal artery of rabbit.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 1999년도 학술발표회 진행표 및 논문초록
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• pp.31-31
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• 1999

Neuropeptide ${\gamma}$ is a recently identified tachykinin family peptide which has conserved ammo acid sequence of -Phe-X-Gly-Leu-Met-NH2 in the C-terminal region, where X represents aromatic or hydrophobic residues. In this study, three-dimensional structure of neuropeptide ${\gamma}$ from goldfish Carassius auratus (G-NP${\gamma}$) was determined by NMR spectroscopy.(omitted)

• Toxicological Research
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• 제31권4호
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• pp.415-420
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• 2015

The purpose of the present study is to investigate the correlations between food intake behavior and job stress level and neuropeptide hormone concentrations. Job strain and food intake behavior were first identified using a self-reported questionnaire, concentrations of neuropeptide hormones (adiponectin, brain derived neurotrophic factor [BDNF], leptin, and ghrelin) were determined, and the correlations were analyzed. In the results, job strain showed significant correlations with adiponectin (odds ratio [OR], 1.220; 95% confidence interval [CI], 1.001~1.498; p < 0.05) and BDNF (OR, 0.793; 95% CI, 0.646~0.974; p < 0.05), and ghrelin exhibited a significant correlation with food intake score (OR, 0.911; 95% CI, 0.842~0.985, p < 0.05). These results suggest that job stress affects food intake regulation by altering the physiological concentrations of neuropeptide hormones as well as emotional status.

• BMB Reports
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• 제48권12호
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• pp.645-646
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• 2015

The sympathetic nervous system (SNS) or neurotransmitters in the bone marrow microenvironment has been known to regulate hematopoietic stem cell (HSC) functions such as self-renewal, proliferation and differentiation. However, the specific role of neuropeptide Y (NPY) in this process remains relatively unexplored. In this study, we demonstrated that NPY deficient mice have significantly reduced HSC numbers and impaired bone marrow regeneration due to apoptotic destruction of SNS fibers and/or endothelial cells. Moreover, NPY treatment prevented bone marrow impairments in a mouse model of chemotherapy-induced SNS injury, while conditional knockout mice lacking the Y1 receptor in macrophages did not restore bone marrow dysfunction in spite of NPY injection. Transforming growth factor-beta (TGF-β) secreted by NPY-mediated Y1 receptor stimulation in macrophages plays a key role in neuroprotection and HSC survival in the bone marrow. Therefore, this study reveals a new role of NPY in bone marrow HSC microenvironment, and provides an insight into the therapeutic application of this neuropeptide.