• 제목, 요약, 키워드: nitrite scavenging effect

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해조류에 의한 아질산염 소거활성 분석-연구노트- (Determination of Nitrite-scavenging Activity of Seaweed)

  • 박영범
    • 한국식품영양과학회지
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    • v.34 no.8
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    • pp.1293-1296
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    • 2005
  • 일상 식생활에서 널리 섭취하고 있는 식용 및 미식용인 16종의 해조류를 사용하여 니트로사민의 직접적인 생성인자인 아질산염 소거작용에 관하여 검토하였다. 그 결과, 갈조류의 경우가 녹조류 및 홍조류에 비해 우수한 분해효과를 나타내었으며 갈조류 중에서도 다시마과의 감태, 곰피, 구멍쇠 미역이 뛰어난 아질산염 분해효과를 나타내었다. 또한 각 시료 모두 pH의존성이 크게 나타나 pH 4.2 및 6.0에서 보다 pH 1.2에서 아질산염 분해효과가 뛰어났으며 아질산염 분해효과가 우수할수록 환원력이 높아 아질산염 분해능과 환원력은 밀접한 관련성을 나타내었다.

일부 페놀성 화합물의 항산화효과 및 아질산염 소거능 (Antioxidative Activities and Nitrite-scavenging Abilities of Some Phenolic Compounds)

  • 안선일;복진흥;손종연
    • 한국식품조리과학회지
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    • v.23 no.1
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    • pp.19-24
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    • 2007
  • This study investigated the antioxidant and synergistic effects and nitrite scavenging ability of some phenolic compounds(catechin, rutin, quercetin and naringin), The electron donating abilities of naringin, quercetin, rutin and catechin were 6.7%, 92.8%, 87.6% and 92.21%, respectively, The antioxidant activities in O/W emulsion substrates were in order of rutin > quercetin > catechin > naringin. The antioxidant effect of rutin was stranger than that of BHT or ${\alpha}$-tocopherol. ${\alpha}$-tocopherol showed synergistic effect with catechin and quercetin, but ascorbic acid not showed effect. The nitrite scavenging abilities of catechin, quercetin, rutin and naringin were 99.9%, 98.6%, 25.5% and 0.2%, respectively. The nitrite scavenging abilities of quercetin and actechin were very potent as compared with those of BHT and ascorbic acid.

The Screening of Nitrite Scavenging Effect of Marine, Algae and Active Principles of Ecklonia Stolonifera

  • CHOI Jae Sue;LEE Ji Hyeon;JUNG Jee Hyung
    • 한국수산과학회지
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    • v.30 no.6
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    • pp.909-915
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    • 1997
  • The nitrite scavenging effect of methanol extracts of marine algae were evaluated to discover new natural nitrite scavengers. Among the tested seaweeds, Ecklonia stolonifera, an edible brown algae, showed the strongest scavenging effect. The MeOH extract was then sequentially partitioned into $CH_2Cl_2,\;CH_2Cl_2$ insoluble interface, EtOAc, n-BuOH, and $H_2O$ layers. The EtOAc and n-BuOH fraction demonstrated high levels of nitrite-scavenging activity while the $CH_2Cl_2,\;CH_2Cl_2$ insoluble interface, and $H_2O$ fractions were inactive. A column chromatography of the EtOAc fraction through silica gel and Sephadex LH-20 yielded phloroglucinol and a new compound tentatively named phlorotannin A. The nitrite scavenging activity of phloroglucinol $(IC_{50}=3.9{\mu}g/ml)$ was more potent than that of L-ascorbic acid $(IC_{50}=65.0{\mu}g/ml)$. However, phlorotannin A $(IC_{50}=193.2{\mu}g/ml)$ showed only low levels of activity. From the above results, it is possible to suggest that both the MeOH extract and their fractions and isolated phloroglucinol and phlorotannin A obtained from E. stolonifera may be applicable as scavengers of nitrite, which is a precursor for the formation of carcinogenic N-nitroso compounds.

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녹즙추출물의 아질산염 소거능에 대한 연구 (Nitrite Scavenging Effect of Methaol Fraction Obtained from Green Yellow Vegetable Juices)

  • 정소영;김낙경;윤선
    • 한국식품영양과학회지
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    • v.28 no.2
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    • pp.342-347
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    • 1999
  • The objectives of this study were to investigate biological activities such as nitrite scavenging effect of water and methanol fractions obtained from each green juice(Angelica keiskei, kale, carrot) under different pH conditions, and antioxidant vitamin contents in green juice made of green yellow vegetables. The result shows that nitrite scavenging effect of each green juice was 14.0~91.2% in model system. The methanol fraction provided higher effect than the water fraction. The nitrite degradation was the highest at pH 1.2. Nitrite scavenging effect of methanol fraction obtained from kale juice was 91.2% at pH 1.2 which was the highest among tested samples in this experiments. Consequently, extracts of kale juice were shown to provide the greatest functional properties among the vegetables tested. Contents of vitamine E and C in the extracts of kale were higher than the others.

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미나리과 산채의 아질산염 소거능 (Nitrite Scavenging Effect of Umbelliferaeceae)

  • 노광석;양미옥;조은자
    • 한국식품조리과학회지
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    • v.18 no.1
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    • pp.8-12
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    • 2002
  • 미나리과 산채인 참나물, 신선초, 방풍의 조단백은 22.99~31.00%, 조지방은 3.51~5.08%였으며, $\beta$-carotene은 5.40~19.81 mg%, ascorbic acid는 미량 함유하였고, Selenium은 모든 시료에서 검출되지 않았다. 총 페놀 함량은 617.87~875.77 mg%를 나타내었으며, 이 중 방풍이 가장 높은 함량을 보였다. 참나물, 신선초, 방풍의 지방산 조성은 전 지방산 중 linoleic acid와 linolenic acid가 66.46~77.44%를 차지하였다. 아질산염 소거능은 참나물, 신선초, 방풍 모두에서 메탄을 가용성 획분이 수용성 획분에 비하여 높은 소거능을 보였고, pH 1.2〉 4.2〉6.0 순으로 소거능이 높았다. 방풍의 메탄을 가용성 획분의 아질산염 소거능이 pH 1.2에서 95.3%로 가장 높았으며, 다음이 참나물, 신선초 순이었다. 총 페놀과 아질산염 소거능은 높은 상관관계를 나타내었다.

Nitrite Scavenging Effect by Flavonoids and Its Structure-Effect Relationship

  • Choi, Jae-Sue;Park, Si-Hyang;Choi, Jin-Ho
    • Archives of Pharmacal Research
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    • v.12 no.1
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    • pp.26-33
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    • 1989
  • Nineteen flavonoids, five phenolics, two coumarins, maltol and L-ascorbic acid were tested as scavenger of nitrite which is believed to participate in the formation of N-nitroso compounds. Many were found to be potent scavenger and the five most potent ones were (+)-catechin, (-)-epicatechin, phloroglucinol, caffeic acid and L-ascorbic acid. The nitrite scavenging effect was higher at pH 1.2 than pH 3.0 and increased when the incubation time was longer. The possible relationship of structures to scavenging effect of the flavonoids tested was discussed.

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대두, 메주 및 된장의 항산화 효과와 아질산염 소거 효과 (Antioxidant and Nitrite Scavenging Effect of Soybean, Meju and Doenjang)

  • 최계선;임선영;최재수
    • 생명과학회지
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    • v.8 no.5
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    • pp.473-478
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    • 1998
  • 본 연구에서는 된장의 기능성 중에서 대두, 메주, 된장의 발효과정을 거치는 동안 항산화 효과와 아질산염 소거 효과의 변화 추이를 고찰하기 위해 DPPH radical 소거능과 아질산염 소거능을 통한 된장의 활성을 시험하였다. 대두, 메주 및 된장은 methanol extract를 계통 추출법에 의거하여 여러 가지 용매를 사용하여 분획하였으며, 각 분획에 대한 활성실험을 행하였다. MeOH ext.로 본 항산화 효과는 된장〉메주〉대두의 순으로 나타났고, 메주와 대두 간의 효과의 차는 거의 없었다. 아질산염 소거효과는 된장〉대두〉메주의 순을 보였고, 래디칼 소거능보다 아질산염 소거능이 더 효과적으로 나타났다. Ethyl acetate 분획에 있어 항산화 효과가 켰으며, 특히 아질산염의 소거 효과는 L-ascorbic acid의 효과를 상회하였다.이에 된장의 ethyl acetate 분획을 반복 정제하여 genis-tin, daidzein, genistein을 분리하였으며, 이는 된장의 항산화 및 아질산염 소거 효과를 나타내는 활성 물질들은 대두중의 페놀성물질에서 크게 벗어나지 않음을 알 수 있었다.

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국내산 키위의 색상별 항산화능 및 아질산염 소거능 비교 (Comparison of Antioxidant and Nitrite Scavenging Activities of Different Colored Kiwis Cultivated in Korea)

  • 정해정;김천제;최윤상
    • 한국식생활문화학회지
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    • v.30 no.2
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    • pp.220-226
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    • 2015
  • This study was to conducted to investigate the antioxidative activity of 70% ethanol extracts of kiwi of three different colors (gold, green and red) by measuring DPPH, ABTS radical scavenging activity, tyrosinase inhibitory effect, metal chelating effect, reducing power, and nitrite scavenging activity. Total polyphenol contents were: gold kiwi, 3.09 mg GAE/g, green kiwi 2.71 mg GAE/g, and red kiwi 4.59 mg GAE/g, respectively. Red kiwi showed higher antioxdidant activity than gold and green kiwi. DPPH and ABTS radical scavenging activity, and nitrite scavenging activity of red kiwi exhibited 94.83, 99.57, and 97.88%, respectively, at a concentration of 20 mg/mL, which were equal to those of ascorbic acid (positive control). Metal chelating effect of red kiwi was superior to that of ascorbic acid. Therefore, the availability of red kiwi will be increased in the field of functional material for food additives and value added products.

석이버섯 용매 추출물의 항산화 및 아질산염 소거작용 (Antioxidative and Nitrite-scavenging Effects of Solvent Extracts from Gyrophora esculenta)

  • 정은재
    • 한국식품영양학회지
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    • v.11 no.4
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    • pp.426-430
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    • 1998
  • This study was conducted to investigate electron donating ability, antioxdative activity and nitrite-scavenging effect of solvent extracts from Gyrophora esculenta. The phenolic compounds of diethylether, butanol, petroleum ether and hexane extracts were 194.20$\mu\textrm{g}$/ml, 98.80$\mu\textrm{g}$/ml, 1.60$\mu\textrm{g}$/ml and 0.20$\mu\textrm{g}$/ml, respectively. Electron donating abilities of diethyleter and butanol extracts from Gyrophora esculaenta were 80.79% and 72.81%, respectively. Absorption of DPPH by diethyleter and butanol extracts sharply increaed at initial stage. Antioxidative activities of fiethylether and butanol extracts by peroxide values are rather high as compared to those of control, but low s compared to those of BHA. Nitrite-scavenging abilities of diethylether, butanol and petroleum ether from Gyrophora esculenta were 96.07%, 77.39% and 15.07%, respectively.

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감마선 조사한 오미자 추출물의 아질산염 소거능, Tyrosinase와 Xanthine Oxidase 저해 효과 (Inhibitory Effect on Tyrosinase and Xanthine Oxidase, and Nitrite Scavenging Activities of Schizandrae Fructus Extract by Gamma Irradiation)

  • 전태욱;조철훈;김기혁;변명우
    • 한국식품저장유통학회지
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    • v.9 no.4
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    • pp.369-374
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    • 2002
  • 오미자 추출물에 감마선을 조사하여 tyrosinase 저해, xanthine oxidase 저해 및 아질산염 소거능에 대하여 검토하였다. 오미자 추출물은 열수, ethanol, methanol 그리고 acetone등을 이용하여 추출하였으며, 감마선은 10, 20과 30 kGy로 조사하였다. 오미자 추출물은 모두 tyrosinase 저해 효과를 가지고 있는 것으로 나타났다. 감마선 조사 후 오미자 추출물에 대한 tyrosinase는 열수 추출물보다 용매 추출물이 저해 효과가 높게 나타내었다. Xanthine oxidase 저해 효과실험 결과, 오미자 추출물은 열수 및 용매 추출물 모두 저해력을 나타내었으며, 감마선 조사는 xanthine oxidase 저해력에 영향을 미치지 않았다. 아질산염 소거능은 pH 1.2, 3.0 그리고 6.0으로 측정하였다. 오미자 추출물은 pH 1.2와 3.0 에서 높은 소거력을 나타내었고, 열수 추출물은 methanol, ethanol 그리고 acetone 추출물보다 아질산염 소거능이 훨씬 높게 나타내었다. 감마선을 30 kGy까지 조사하였을 때, 오미자 추출물의 생리 활성에는 크게 영향을 미치지 않았다.