• Title, Summary, Keyword: nitrite scavenging effect

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Effect of Germinated Brown Rice Concentrate on Free Amino Acid Levels and Antioxidant and Nitrite Scavenging Activity in Kimchi

  • Woo, Seung-Mi;Jeong, Yong-Jin
    • Food Science and Biotechnology
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    • v.15 no.3
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    • pp.351-356
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    • 2006
  • In this study, we investigated the effect of adding a 1, 3, or 5% solution of germinated brown rice concentrate (GBRC) to fermented kimchi. During fermentation, the concentration of free amino acids and essential amino acids increased with increasing concentrations of GBRC. In particular, higher levels of free amino acids were associated with a sweet taste compared with controls. The ${\gamma}$-aminobutyric acid (GABA) content of kimchi containing the 5% GBRC solution was 3 times higher than that of controls. The total phenolic compound content (130 mg%) did not change significantly in the control group, but increased in 10 mg% increments as the GBRC concentration rose from 1 to 3 to 5%. 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging activity and superoxide radical scavenging activity also increased with the GBRC concentration, with maximum activity during the ripe stage with GBRC measured at 79 to 82% compared with controls (30 to 71 %). The nitrite scavenging activity was 10% higher with GBRC compared with controls and was highest when the pH was 1.2. These results showed that the addition of GBRC is effective in improving the function of kimchi.

Physiological Activity and Antioxidative Effect of Methanol Extract from Onion (Allium cepa L.) (양파 메탄올 추출물의 생리활성 및 항산화효과)

  • 김현구;곽희진;권영주;정필호;권중호
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.2
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    • pp.349-355
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    • 2000
  • Onion was extracted by methyl alcohol and some physiological activities and antioxidative effect of the extracts on soybean oil, corn oil, lard and shortening were compared with commercial antioxidants such as $\alpha$-tocopherol and BHT. The electron donating ability was about 70% at concentration above of 30mg/mL. Nitrite scavenging effects of all concentrations diminished at higher pH, while in the case of pH 1.2, it showed a nitrite scavenging effect of more than 80% af concentration above of 30mg/mL. And also, the angiotensin I-converting enzyme inhibitory effect was good above 80%. The soybean oil, corn oil, lard and shortening containing different levels of the onion extract were stored at 6$0^{\circ}C$ to evaluate the antioxidative activity of onion extracts. Peroxide value, acid value and TBA value of each oil were detected. Onion extract was very effective to retard oxidation of soybean oil, corn oil and lard, and especially, the very high antioxidative effect was observed for lard.

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Physiological Functionality and Nitrite Scavenging Ability of Fermentation Extracts from Pine Needles (솔잎발효추출물의 효소적 저해활성 및 아질산염 소거작용)

  • 홍택근;이용림;임무현;정낙현
    • Korean Journal of Food Preservation
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    • v.11 no.1
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    • pp.94-99
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    • 2004
  • Effects on the physiological functionality, such as tyrosinase, xanthine oxidase, angiotensin converting enzyme and Nitrite scavenging ability were also observed by pine needle fermentation extract(PFE) and the difference in the consistency of pine needle ethanol extracts(PE 80, PE 50) was found. In the inhibition effect on tyrosinase, PFE showed 5-38% higher than that of PE 80 and PE 50. In the inhibition on XOase, PFE, PE 80 and PE 50 showed 62.77%, 64.90%, 55.9% respectively. In the inhibition effect on ACE, PFE, PE SO and PE 50 showed 78.02%, 69.82% and 21.75% respectively. Among these, PFE showed the highest ACE inhibition effect. In the inhibition effect on nitrite scavenging ability, the pine needle extracts showed a high effect in pH 3.0. As the result of the research using HPLC for the organic acid, all the samples(PFE, PE 80, PE 50) showed higher contents of the ascorbic acid concerned with the effect of the antioxidative. PFE showed the highest contents of the ascorbic acid.

Inhibitory Effect on Tyrosinase, ACE and Xanthine Oxidase, and Nitrite Scavenging Activities of Jubak (Alcohol filter cake) Extracts (주박 추출물의 아질산염 소거와 Tyrosinase, Xanthine Oxidase, ACE 저해 효과)

  • Kwon, Sang-Chul;Jeon, Tae-Woog;Park, Jeong-Seob;Kwak, Joon-Soo;Kim, Tae-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.9
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    • pp.1191-1196
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    • 2012
  • The purpose of this study was to investigate the tyrosinase, xanthine oxidase, angiotensin converting enzyme (ACE) inhibitory effects, and nitrite scavenging abilities of Jubak (alcohol filter cake, AFC) hot water and ethanol extracts. Tyrosinase inhibitory effects of Jubak hot water and ethanol extracts were above 85%. Nitrite scavenging ability was higher at pH 1.2 than at pH 4.2 and 6.0. Nitrite scavenging abilities of water and ethanol extracts were above 90% at pH 1.2. Xanthine oxidase inhibitory effect of Jubak hot water and ethanol extracts showed a lower, and the effect did not different by hot water and ethanol. ACE inhibitory effects of Jubak hot water and ethanol extracts were approximately 43~53% and 36~47%, respectively. Overall, our results indicate that Jubak hot water and ethanol extracts may have tyrosinase and ACE inhibitory effects and nitrite scavenging ability, but no xanthine oxidase inhibitory effect. Therefore, Jubak has potential as a cosmetic raw material. Additionally, Jubak could be used as a food additive.

Functional Characteristics from the Barley Leaves and its antioxidant mixture - Study on the Nitrite Scavenging Effect - (맥엽 및 항산화 조성물의 생리 기능적 특성에 관한 연구 - 아질산염 소거능을 중심으로 -)

  • Hong, I-Jin;Park, Hyeung-Geun;Jew, Sang-Sup;Kim, Kyung-Tack;Lee, Sung-Hee
    • Applied Biological Chemistry
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    • v.46 no.4
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    • pp.333-337
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    • 2003
  • This study was conducted to estimate the biofunctional characteristics, namely nitrite scavenging effect of barley leaves (BL) and their mixture (AM) with other antioxidants. Aqueous or methanol extracts were obtained from BL and AM. Aqueous and methanol extracts from barley leaves had relatively higher nitrite scavenging effects and its activities and contents increased in a concentration-dependent manner. The activities and contents of methanol extracts obtained from BL and AM were higher than those of aqueous extracts. Especially, AM containing BL and other antioxidant mixture had the highest activities and contents increased in a concentration-dependent manner. BL or AM were added to macrophage cells of RAW 264.7. Survival rates of the cells treated with BL and AM were measured to be different. $IC_{50}$ value decreased in a concentration-dependent manner by the addition of aqueous or methanol extracts from BL and AM. Especially, methanol extracts of AM had the highest nitrite scavenging effects. Thus, BL and AM may have protective effect against carcinogen and immune ability against reactive molecules through NO (nitric oxide) signal pathway. From the above results, barley leaves appear to contain natural anticancer and immune-related agents and may have potentiality to be used as functional food ingredients.

Effect of the N-nitrosodimethylamine Formation in Ascorbate and Phenolic Portions from Citrus Juice (감귤류의 Ascorbate 및 Phenolic 획분이 N-nitrosodimethylamine의 생성에 미치는 영향)

  • 성낙주;송미향;이수정;신정혜;최선영
    • The Korean Journal of Food And Nutrition
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    • v.15 no.2
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    • pp.97-103
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    • 2002
  • Five citrus juices were separated into a ascorbate and phenolic portion using rep-pak C$\_$18/ cartridge, respectively, in order to elucidate the nitrite scavenging effect and N-nitrosodimethylamine(NDMA) formation in model system. The nitrite scavenging effect of ascorbate portion from citrus juices, in the different pH, when added with 5ml were 79.9 ∼98.6% under the condition of pH 2.5. 48.5∼86.3% at pH 4.2 and lower than 35.2% at pH 6.0. The nitrite scavenging effect was excellent phenolic portion rather than ascorbate portion. Particularly, the effect was more 2 times than ascorbate portion under the reaction condition of pH 6.0. When added the phenolic portion in the reaction mixture, NDMA formation was inhibited 92.8% or more in kum quat, mandarin orange and sweet orange juices. But the ascorbate portion was a negative response of the inhibition of NDMA formation. The inhibition on NDMA formation in citrus juice may be due to phenolic compounds were reacted.

Nitrite-scavenging Effects of Roasted-barley Extracts according to Processing Conditions (가공조건에 따른 볶음보리 추출물의 아질산염 소거작용)

  • Kim, Seon-Bong;Do, Jeong-Ryong;Lee, Yong-Woo;Gu, Yeun-Sug;Kim, Chang-Nam;Park, Yeung-Ho
    • Korean Journal of Food Science and Technology
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    • v.22 no.7
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    • pp.748-752
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    • 1990
  • This paper was investigated to elucidate the nitrite-scavenging ability of roasted-barley extracts. Roasted-barley extracts exhibited nitrite-scavenging ability. The nitrite-scavenging ability of roasted-barley extracts showed pH-dependent, and did the highest at pH 1.2 and the lowest at pH 6.0. The roasting temperature and time of barley examined influenced the nitrite-scavenging ability of roasted-barley extracts. The water soluble fraction obtained from barley roasted at $230^{\circ}C$ for 90 min showed higher the nitrite-scavenging effect than the others. Ethanol soluble fraction which is further fractionated from water soluble fraction was most effective in nitrite-scavenging.

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Different of Total Phenolics and Flavonoids, Radical Scavenging Activities and Nitrite Scavenging Effects of Momordica charantia L. According to Cultivars (여주 품종별 폴리페놀, 플라보노이드 함량과 라디칼 소거활성 및 아질산염 소거능)

  • Boo, Hee-Ock;Lee, Hyun-Hwa;Lee, Jang-Won;Hwang, Sung-Jin;Park, Sang-Un
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.1
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    • pp.15-20
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    • 2009
  • This study was carried out in order to determine the biological activities such as antioxidant activities in nine cultivars of Momordica charantia L. (Korea, China, Japan and Philippine native cultivars). Their antioxidant activities were measured using DPPH free radical scavenging, ABTS cation radical scavenging, and Nitrite scavenging ability. The highest total polyphenol content (16.82 ${\mu}g/m{\ell}$) measured in the native Korea cultivar, and this value was 4.0 ${\mu}g/m{\ell}$ higher than that of the 'Verde Beuhas' cultivar (12.82 ${\mu}g/m{\ell}$). The 'Peacock' cultivar had the highest total flavonoid amount which was 4.38 ${\mu}g/m{\ell}$. The free radical scavenging activity using DPPH method was the highest in the native China cultivar ($RC_{50}$ = 102.6 ${\mu}g/m{\ell}$). ABTS cation radical scavenging activity according to cultivars was significantly higher in 'Peacock'. Nitrite scavenging ability showed the most remarkable effect at the pH 1.2, exhibited to 81.5${\sim}$86.9% by addition of ethanol extract 1${\mu}g/m{\ell}$ from Momordica charantia L. These results suggest that Momordica charantia L. had the potent biological activities, and that their activities exhibited differently depending on cultivars.

Functional Activities of Microwave-Assisted Extracts from Lyophyllum ulmarium (마이크로웨이브 추출공정에 의한 만가닥버섯의 기능적 특성)

  • 김현구;최윤정;정승원;김공환
    • Korean Journal of Food Preservation
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    • v.9 no.4
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    • pp.385-390
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    • 2002
  • Functional activities of Lyophyllum ulmarium microwave-assisted extracts under different conditions including electron donating ability, tyrosinase inhibition activity and nitrite scavenging effect were examined. Total polyphenol content increased as increasing microwave power up to 90 W in the water extracts. Electron donating ability increased with microwave power up to 90 W in 50% ethanol extract and 99% ethanol extract. Tyrosinase inhibition activity and nitrite scavenging effect in the extract increased as microwave power increased during extraction. Total polyphenol content increased as extending extraction time up to 5 min in the water extract. But the highest electron donating ability and tyrosinase inhibition activity was obtained after 10 min extraction. Significantly higher total polyphenol content and electron donating ability were found in the water extract whereas greater tyrosinase inhibition activity and nitrite scavenging effect were observed in 99% ethanol extract. The maxium nitrite scavenging effect was found at pH 1.2 and decreased as pH increased.

Functional Activities of Microwave-Assisted Extracts from Flammulina velutipes (마이크로웨이브 추출공정에 의한 팽이버섯 추출물의 기능적 특성)

  • Kim, Hyun-Ku;Choi, Yoon-Jung;Kim, Kong-Hwan
    • Korean Journal of Food Science and Technology
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    • v.34 no.6
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    • pp.1013-1017
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    • 2002
  • Functional activities of Flammulina velutipes extract including electron donating ability, nitrite-scavenging effect, and tyrosinase inhibition activity was examined. Extraction were carried out by microwave-assisted extraction (MAE) under different conditions including solvent and microwave power. Tyrosinase inhibition activity and nitrite-scavenging effect increased as microwave power increased during extraction. Total phenol content and electron-donating ability reached maximum at the microwave power of 90 W. Total polyphenol content and electron-donating ability increased as extraction time extended up to 15 min, with the highest tyrosinase inhibition obtained after 5 min extraction. Significantly higher tyrosinase inhibition activity was found in 99% ethanol extract, whereas greater nitrite-scavenging effect was observed in the water extract. The maximum nitrite-scavenging effect was found at pH 1.2 and decreased as pH increased.